Papers by Douglas Evangelista
Ponto Urbe, 2015
Shopping-chão: identidade e circulação de pessoas e objetos em uma feira de "...
In the century XXI, the environmental issue is spread through the national and international scen... more In the century XXI, the environmental issue is spread through the national and international scenery. There are many elements that legitimate this affirmation and one of them is the existence of international political arrangements that focus on environmental thematic. In this contemporaneous context the “International Regime on Biodiversity” (IRB) emerges. This regime has the objective on international politics of “preservation”, “conservation” and “sustainable” organization of the biodiversity in planetary level. In this way, the thematic of this paper are the Institutions and International regimes. However, the international political organization has many ways to active that stimulate its creation. That happens because, this paper focus on State conduct, in special, Brazil, on activing the fundamental objectives of IRB. One way to get what the IRB orient is by the twenty “Aichi Targets”, which began in 2011 and will finish in 2020. So, is necessary to ask: which points, among th...
Resumo: O Regime Internacional de Mudanças Climáticas (RIMC) é um dos principais fóruns de debate... more Resumo: O Regime Internacional de Mudanças Climáticas (RIMC) é um dos principais fóruns de debate sobre o meio ambiente na contemporaneidade, posto que este arranjo da política internacional oriente o comportamento de seus membros. Isto posto, o objetivo central desde trabalho é estudar a influência do RIMC na Lei 3.135 de Mudanças Climáticas do Amazonas. Para tal é proposto a seguinte pergunta: como as diretrizes do RIMC influenciaram a criação de políticas públicas do Estado do Amazonas nas questões ambientais, no período de 2007 a 2009?A resposta provisória desta indagação é: há um constrangimento progressivo, pois os princípios e normas do RIMC são da década de 90 e suas políticas só tomam corpo a partir de 2007 na região do Amazonas. Desta maneira, o trabalho será dividido em duas partes. Na primeira parte será feita uma revisão de literatura e, por consequente, na próxima etapa será feita uma análise propriamente dita. Palavras-chaves: Mudanças Climáticas; Lei 3.135 do Estado ...
Biotransformation of Colchicinoids into Their Corresponding 3-O-Glucosyl Derivatives by Selected Strains of Bacillus megaterium
Molecular Biotechnology, 2014
Natural colchicinoids and their semisynthetic derivatives are important active ingredients for ph... more Natural colchicinoids and their semisynthetic derivatives are important active ingredients for pharmaceutical applications. Thiocolchicoside (3-demethoxy-3-glucosyloxythiocolchicine) is used in several countries as standard therapy for the treatment of diseases of the muscle-skeletal system, due to its potent antiinflammatory and myorelaxant properties. Manufacturing of thiocolchicoside requires a key step, the regioselective demethylation and glucosylation of chemically derivative thiocolchicine. High selectivity and efficiency of this transformation cannot be achieved in a satisfactory way with a chemical approach. In particular, the chemical demethylation, a part from requiring toxic and aggressive reagents, generates a complex mixture of products with no industrial usefulness. We report herein an efficient, direct and green biotransformation of thiocolchicine into thiocolchicoside, performed by a specific strain of Bacillus megaterium. The same process, with minor modifications, can be used to convert the by-product 3-O-demethyl-thiocolchicine into thiocolchicoside. In addition, we describe the B. megaterium strain selection process and the best conditions for this effective double biotransformation. The final product has a pharmaceutical quality, and the process has been industrialised.
Biotransformation of Colchicinoids into Their Corresponding 3-O-Glucosyl Derivatives by Selected Strains of Bacillus megaterium
Molecular Biotechnology, 2014
Natural colchicinoids and their semisynthetic derivatives are important active ingredients for ph... more Natural colchicinoids and their semisynthetic derivatives are important active ingredients for pharmaceutical applications. Thiocolchicoside (3-demethoxy-3-glucosyloxythiocolchicine) is used in several countries as standard therapy for the treatment of diseases of the muscle-skeletal system, due to its potent antiinflammatory and myorelaxant properties. Manufacturing of thiocolchicoside requires a key step, the regioselective demethylation and glucosylation of chemically derivative thiocolchicine. High selectivity and efficiency of this transformation cannot be achieved in a satisfactory way with a chemical approach. In particular, the chemical demethylation, a part from requiring toxic and aggressive reagents, generates a complex mixture of products with no industrial usefulness. We report herein an efficient, direct and green biotransformation of thiocolchicine into thiocolchicoside, performed by a specific strain of Bacillus megaterium. The same process, with minor modifications, can be used to convert the by-product 3-O-demethyl-thiocolchicine into thiocolchicoside. In addition, we describe the B. megaterium strain selection process and the best conditions for this effective double biotransformation. The final product has a pharmaceutical quality, and the process has been industrialised.
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Papers by Douglas Evangelista