Skip to main content

Desh Deepak

Follower

1

Following

12

Public Views

Phone: 96279 83508
Address: Villege : Behedeki Saidabaad, Post: Iqbalpur, Distt: Haridwar, Roorkee, 247668

less

Marcelo Negri Solha

Paolo Fraticelli

Alessandra Cancellieri

University of Louisville, KY

Baghiyatollah Medical University

Interests

Uploads

Papers by Desh Deepak

Recognition of Small Airways Obstruction in Asthma and COPD - The Road Less Travelled

JOURNAL OF CLINICAL AND DIAGNOSTIC RESEARCH, 2017

Conventional spirometry: It is a tool used to diagnose airway obstruction and Maximum Mid-Expirat... more Conventional spirometry: It is a tool used to diagnose airway obstruction and Maximum Mid-Expiratory Flow (MMEF) or FEF25-75 is considered to be an indicator of small airways. However, it is highly dependent of Forced Vital Capacity (FVC) and may give paradoxical results after bronchodilation. Therefore, it needs to be obtained at the same FVC. Plethysmography: It measures residual volume and airway resistance as well as RV/TLC. It is simple to perform, sensitive and easily available however, it has low specificity for small airway changes and procedure is time consuming [9]. Basic HRCT chest protocol includes a slice thickness of approximately 0.625-1.25 mm, kV= 120, mAs =100-200, collimation= 1.5-3 mm, scan time= 0.1 sec, high spatial resolution reconstruction algorithm and a matrix size of 768 x 768 or largest possible with a field of view approximately 35 cm. Scanning is usually performed in supine position except in cases of suspected early interstitial changes and to differentiate between early interstitial changes and

Medical management of Dipylidium caninum infected female cat: A case report

International Journal of Chemical Studies, 2019

A female cat aged 2 year was presented to the Referral Veterinary Polyclinic, IVRI Izatnagar with... more A female cat aged 2 year was presented to the Referral Veterinary Polyclinic, IVRI Izatnagar with complaint of continuous crying, weakness, inappetence, discomfort and anal rubbing. Physical examination of cat revealed flea infestation and faecal examination revealed the presence of multiple egg packets containing gravid segments. The systematic investigation showed reduced haemoglobin concentration and total erythrocyte count, but no significant changes in normal biochemical parameters. The institution of treatment to the cat with praziquantel along with the supportive therapy brought successful recovery in 7 days.

Typical Carcinoid: Cytological Diagnosis of a Case and Mimickers in Cytological Sampling Techniques with Brief Review of Literature

Annals of Pathology and Laboratory Medicine, 2019

Typical carcinoids are the most well differentiated and least aggressive of pulmonary neuroendocr... more Typical carcinoids are the most well differentiated and least aggressive of pulmonary neuroendocrine tumors. Cytologic diagnosis on trans bronchial needle aspirate (TBNA) smears is quite diagnostic; however, may be challenging on other cytologic specimens like bronchial brushings and bronchial washings. We discuss such a case of typical carcinoid in a 30 years old female.

Therapeutic Management of Ivermectin Toxicity in a Calf: A Case Report

International Journal of Current Microbiology and Applied Sciences, 2018

Localized laryngotracheobronchial amyloidosis: Management issues

Lung India, 2019

Pulmonary amyloidosis can have four different manifestations: diffuse parenchymal amyloidosis, no... more Pulmonary amyloidosis can have four different manifestations: diffuse parenchymal amyloidosis, nodular parenchymal amyloidosis, infiltrative tracheobronchial amylo idos i s , and nodu la r t r acheobronch ia l amyloidosis.[1] Our patient had localized nodular tracheobronchial amyloidosis associated with bilateral laryngeal nodular amyloid deposits on false vocal cords. Diffuse nodularity and thickening in the tracheobronchial tree along with areas of bronchial narrowing may result in complications such as atelectasis and postobstructive pneumonia.

Pulmonary Hamartoma Mimicking Malignancy: A Cytopathological Diagnosis

JOURNAL OF CLINICAL AND DIAGNOSTIC RESEARCH, 2016

A 53-year-old male presented to medicine outpatient department with complaints of cough with sput... more A 53-year-old male presented to medicine outpatient department with complaints of cough with sputum for last four months. The patient gave a history of chronic smoking and change in voice. However, there was no history of fever, dyspnea, haemoptysis, dysphagia, loss of appetite and weight loss. On general examination, there was absence of pallor, cyanosis, clubbing, oedema and any peripheral lymphadenopathy. On examination of the respiratory system mild left sided ronchi was noted on auscultation, however, bilateral air entry was normal. Rest of the systems examined did not reveal any abnormality.

Extracorporeal treatment with high-volume continuous venovenous hemodiafiltration and charcoal-based sorbent hemoperfusion for severe metformin-associated lactic acidosis

Indian Journal of Critical Care Medicine, 2016

We present a case of a 49-year-old female with an alleged history of ingestion of approximately 1... more We present a case of a 49-year-old female with an alleged history of ingestion of approximately 100 tablets of metformin (850 mg each). Investigations revealed severe lactic acidosis with lactate levels of 13.5 mmol/L and pH of 7.17. This indicates severe toxicity and is associated with a high mortality. Charcoal-based sorbent hemoperfusion was done as a desperate effort, as patient continued to deteriorate despite supportive care and high-volume continuous venovenous hemodiafiltration. The patient survived despite metformin-associated lactic acidosis related to severe metformin toxicity.

Spontaneous Expulsion of a Hydatid Cyst During Flexible Bronchoscopy

Journal of Bronchology, 2006

Wolters Kluwer Health may email you for journal alerts and information, but is committed to maint... more

ChemInform Abstract: Absolute Configuration of Calogenin (I) and Its 20-Keto Derivative (II)

ChemInform, 2009

The absolute configuration of calogenin (isolated from Periploca calophylla) and its 20-keto deri... more The absolute configuration of calogenin (isolated from Periploca calophylla) and its 20-keto derivative (isolated from Hemedesmus indicus) has been established as pregn-5-ene-3β,14β,20R triol containing a C-17 hydroxy ethyl chain in αconfiguration and pregn-5-ene-20-keto-3β,14β-diol containing a C-17 methyl keto chain in β-configuration, respectively.

C21 steroidal glycosides from Hemidesmus indicus

Phytochemistry, 2000

Two novel pregnane glycosides, denicunine (1) and heminine (4), have been isolated from the dried... more Two novel pregnane glycosides, denicunine (1) and heminine (4), have been isolated from the dried stem of Hemidesmus indicus R.Br. (family: Asclepiadaceae). Chemical transformations and spectroscopic evidence viz: 1H and 13C NMR spectroscopy and FABMS are consistent with the structures calogenin 3-O-3-O-methyl-beta-D-fucopyranosyl-(1--&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;gt;4)-O-beta-D-oleandropyranosi de and calogenin 3-O-beta-D-cymaropyranosyl-(1--&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;gt;4)-O-beta-D-digitoxopyranoside+ ++, respectively.

Two pregnane glycosides from Hemidesmus indicus

Phytochemistry, 1991

... 9422/91 $300+000 >( 1990 Pergamon Press pic TWO PREGNANE GLYCOSIDES FROM HEMIDESMUS INDICU... more

Indicusin-A Pregnane Diester Triglycoside from Hemidesmus indicus R. Br

Natural Product Letters, 1995

A novel pregnane oligoglycoside viz. Indicusin (1) was isolated from CHCl3-EtOH (3:2) fraction of... more A novel pregnane oligoglycoside viz. Indicusin (1) was isolated from CHCl3-EtOH (3:2) fraction of Hemidesmus indicus R Br. (family Asclepiadaceae). With the help of FABMS, H NMR, C NMR spectroscopy and chemical transformations, the structure of 1 was defined as 11α, 12β-di-O-acetyl-orgogenin-3-O-β-D-cymaropyranosyl (1→4)-O-β-D-cymaropyranosyl (1→4)-O-β-D-cymaropyranoside.

Clinical profile, intensive care unit course, and outcome of patients admitted in intensive care unit with dengue

Journal of Critical Care, 2011

The purpose of the study was to assess the clinical profile and course of dengue patients admitte... more The purpose of the study was to assess the clinical profile and course of dengue patients admitted to the intensive care unit (ICU) and to identify factors related to poor outcome. All patients with dengue admitted to ICU over 2.5 years were included prospectively. Severity of illness was assessed by the Acute Physiology and Chronic Health Evaluation (APACHE) II score, and organ failure was determined by the Sequential Organ Failure Assessment score. Primary outcome measure was 28-day mortality. Logistic regression analysis was performed to identify factors predicting mortality. Data from 198 patients were analyzed. Mean age was 39.56 ± 17.1 years, and 61.1% were male. The commonest complaints were fever (96%) and rash (37.9%). Mean admission APACHE II and Sequential Organ Failure Assessment scores were 7.52 ± 7.8 and 4.52 ± 3.4, respectively. The commonest organ failure was coagulation (43.4%) followed by respiratory failure (13.1%). Vasopressors were required by 11.6%; and dialysis and mechanical ventilation were required by 7.6% and 9.1%, respectively. Mortality was 12 (6.1%); and on multivariate analysis, APACHE II score (odds ratio, 1.781; 95% confidence interval, 0.967-3.281; P = .048) could independently predict mortality. Patients with dengue fever may require ICU admission for organ failure. Outcome is good if appropriate aggressive care and organ support are instituted. Admission APACHE II score may predict patients at higher risk of death.

Correlation of oxidative status with BMI and lung function in COPD

Clinical Biochemistry, 2007

Objectives: The imbalance in oxidative status together with nutrition depletion and low body weig... more Objectives: The imbalance in oxidative status together with nutrition depletion and low body weight play a vital role in the pathogenesis and severity of chronic obstructive pulmonary disease (COPD). The study was undertaken to ascertain if a relationship existed between oxidative status and BMI in COPD. In addition, association of oxidative status and BMI with lung function of the disease was also examined. Materials and methods: In 202 COPD patients and 136 healthy controls plasma lipid peroxidation (LPO), reduced glutathione (GSH), glutathione peroxidase (GPx), catalase (CAT) activities, BMI and FEV 1 % predicted were looked for interactions. Results: The patients had increased LPO (p = 0.006) and decreased antioxidants (GSH, p = 0.005; GPx, p = 0.035 and CAT, p = 0.008, respectively). Of note are the correlations of oxidative stress markers with BMI and FEV 1 % predicted in the patients. LPO inversely and GSH, GPx, and CAT positively correlated with both BMI (p = 0.007, p b 0.001, p = 0.045 and p = 0.009, respectively), and FEV 1 % of predicted (LPO, p = 0.001; GSH, p b 0.001; GPx, p = 0.043 and CAT, p b 0.001) in the patients. Further, a positive correlation existed between BMI and FEV 1 % predicted (p = 0.016) in COPD. Conclusion: The intimate relationship of oxidative status with BMI and lung function, and the direct correlation between BMI and FEV 1 may potentiate severity of the disease.

CYP1A1, CYP1A2 and CYBA gene polymorphisms associated with oxidative stress in COPD

Clinica Chimica Acta, 2010

Background: The genetic susceptibility to chronic obstructive pulmonary disease (COPD) depends on... more Background: The genetic susceptibility to chronic obstructive pulmonary disease (COPD) depends on detoxification and antioxidant enzymes, which detoxify cigarette smoke reactive components that, otherwise, generate oxidative stress. Methods: In a case-control study of 346 subjects with and without COPD, we examined the polymorphisms 462Ile/Val, 3801T/C of CYP1A1, −3860G/A of CYP1A2 and −930A/G, 242C/T of CYBA individually or in combination and their contribution to oxidative stress markers by measuring malondialdehyde (MDA), catalase (CAT), glutathione (GSH) and glutathione peroxidase (GPx). Results: COPD patients had significantly increased MDA concentration (p < 0.001) and decreased CAT activity, GSH concentration, GPx activity (p ≤ 0.01). The patients were over-represented by the alleles 462Val, 3801C of CYP1A1 and −930G, 242C of CYBA (p < 0.001, p=0.003, p =0.030 and p = 0.031, respectively) and consequently the haplotypes of same alleles i.e. 462Val:3801C, 462Val:3801T and −930G:242C (p = 0.048, p = 0.016 and p =0.039, respectively). Similarly, CYP1A1 and CYP1A2 haplotypes, 462Val:3860G and 462Val:3801T:3860G were significantly over-represented (p = 0.001 and p =0.003), respectively in patients. The same alleles-associated genotype-combinations between genes were more prevalent in patients. Of note, the genotypes, 462Ile/Val+Val/ Val, 3801TC+CC of CYP1A1 and −930AG+GG of CYBA associated with increased MDA concentration (p = 0.018, p = 0.045 and p =0.017, respectively), decreased CAT activity (p < 0.0001, p =0.080 and p < 0.0001, respectively) and GSH concentration (p < 0.0001, p = 0.0002 and p =0.011, respectively) in patients. Conclusion: The identified alleles, its haplotypes and the genotype-combination along with increased oxidative stress, signify the importance in susceptibility to COPD.

Genetic polymorphisms of GSTP1 and mEPHX correlate with oxidative stress markers and lung function in COPD

Biochemical and Biophysical Research Communications, 2007

The genetic susceptibility to COPD might depend on variations in detoxification enzymes that acti... more The genetic susceptibility to COPD might depend on variations in detoxification enzymes that activate and detoxify cigarette smoke products, which otherwise generate oxidative stress causing pathogenesis. In a case-control study of 202 COPD patients and 136 normals, we examined the association of polymorphisms I105V, A114V of GSTP1 and Y113H, H139R of mEPHX individually or in combination with disease and their contribution to oxidative stress markers such as MDA, GSH, GPx and airflow obstruction. Patients were over-represented by the alleles 105V, 114V of GSTP1 and 113H, 139H of mEPHX (v 2 = 10.63, p = 0.001, v 2 = 13.92, p < 0.001, v 2 = 13.02, p < 0.001 and v 2 = 4.48, p = 0.034, respectively) and the haplotypes of same alleles i.e. 105V-114V and 113H-139H (v 2 = 14.58, p < 0.001 and v 2 = 23.14, p < 0.001). Moreover, there was marked over-representation of combination of genotypes, I105I+A114A of GSTP1 (53% vs. 36%) in controls; whereas, the combinations with 105V/114V alleles (64% vs. 47%) of GSTP1 (OR = 1.99; 95% CI = 1.28-3.09; p = 0.002) and the homozygotes H113H+H139H (27% vs.10%) of mEPHX (OR = 3.26; 95% CI = 1.73-6.15; p = 0.0001) in patients. Patients had significantly elevated MDA level (p < 0.001) and decreased GSH level (p < 0.001) and GPx activity (p = 0.035), respectively. Of note, the genotypes, I105V/V105V, A114V/V114V of GSTP1 and Y113H/ H113H of mEPHX associated with increased MDA level (p = 0.04, p = 0.03 and p = 0.003), decreased GSH level (p = 0.019, p = 0.007 and p = 0.0006) and lower FEV1 (p = 0.23, p = 0.037 and p = 0.029), respectively, in patients; so was the correlation of these biomarkers and lung function with the combinations of the genotypes. In conclusion, 105V/114V alleles of GSTP1 and 113H/139H alleles of mEPHX and the combination of genotypes with same alleles associated with imbalanced oxidative stress and lung function in patients, signifying the importance in the disease.

$Research paper thumbnail of A novel pentasaccharide from immunostimulant oligosaccharide fraction of buffalo milk$

A novel pentasaccharide from immunostimulant oligosaccharide fraction of buffalo milk

Biochimica et Biophysica Acta (BBA) - General Subjects, 1999

A processed oligosaccharide mixture of buffalo milk induced significant stimulation of antibody, ... more A processed oligosaccharide mixture of buffalo milk induced significant stimulation of antibody, delayed-type hypersensitivity response to sheep red blood cells in BALB/c mice. This also stimulated non-specific immune response of the animals measured in terms of macrophage migration index. A novel pentasaccharide has been isolated from the oligosaccharide containing fraction having immunostimulant activity of buffalo milk. This compound was isolated by a combination of gel filtration chromatography, silica gel column chromatography of derivatised oligosaccharides while the homogeneity was confirmed by high performance liquid chromatography. The results of structural analyses, i.e. proton nuclear magnetic resonance, fast atom bombardment mass spectrometry, chemical transformations and degradations are consistent with the following structure : GlcNAcL1 3 3GalL1 3 4GlcNAcL1 3 3GalL1 3 4Glc

Reply by Authors to P. J. Paul

AIAA Journal, 1977

Heterogeneous Combustion of Aluminized Propellants

AIAA Journal, 1976

Hybrid combustion of an aluminized polystyrene/oxygen system has been investigated. The mass cons... more Hybrid combustion of an aluminized polystyrene/oxygen system has been investigated. The mass consumption rate of the fuel is found to be proportional to the square root of the mass consumption rate of the oxidizer. The regression rate depends on time, as was found to be the case for an unmetallized propellant. The role of radiative heat transfer to the combustion products and unreacted oxidizer is confirmed when the data are analyzed in the context of the theory of Marxman et al. Analysis of the data takes into account the heat taken away by metal particles during combustion. This is found to be strongly dependent on the oxidizer flow rate.

Endothermic fuels for supersonic ramjet

Zenodo (CERN European Organization for Nuclear Research), 2003

Greater ease of use and higher density make hydrocarbon fuels an attractive option for hypersonic... more Greater ease of use and higher density make hydrocarbon fuels an attractive option for hypersonic space transport vehicle. In addition to use of fuel for propulsive purpose, it is used for regenerative cooling of system structures. In order to achieve maximum heat sink, endothermic reactions of the hydrocarbon fuels must be employed to augment fuel enthalpy. This should provide cooling sufficient to allow flight in the range of Mach 4-10 at optimum altitude. For a hydrocarbon fuel blend to be suitable for the hypersonic vehicle it should selectively pursue the most beneficial endothermic reaction for optimum cooling and the reaction products should have high combustion intensity under ramjet and scramjet engine conditions. The problem of formation of deposits/coke by hydrocarbons at higher temperature needs to be resolved. The issues for the development of hydrocarbon fuel for hypersonic vehicle are highlighted in this paper.

Recognition of Small Airways Obstruction in Asthma and COPD - The Road Less Travelled

JOURNAL OF CLINICAL AND DIAGNOSTIC RESEARCH, 2017

Conventional spirometry: It is a tool used to diagnose airway obstruction and Maximum Mid-Expirat... more Conventional spirometry: It is a tool used to diagnose airway obstruction and Maximum Mid-Expiratory Flow (MMEF) or FEF25-75 is considered to be an indicator of small airways. However, it is highly dependent of Forced Vital Capacity (FVC) and may give paradoxical results after bronchodilation. Therefore, it needs to be obtained at the same FVC. Plethysmography: It measures residual volume and airway resistance as well as RV/TLC. It is simple to perform, sensitive and easily available however, it has low specificity for small airway changes and procedure is time consuming [9]. Basic HRCT chest protocol includes a slice thickness of approximately 0.625-1.25 mm, kV= 120, mAs =100-200, collimation= 1.5-3 mm, scan time= 0.1 sec, high spatial resolution reconstruction algorithm and a matrix size of 768 x 768 or largest possible with a field of view approximately 35 cm. Scanning is usually performed in supine position except in cases of suspected early interstitial changes and to differentiate between early interstitial changes and

Medical management of Dipylidium caninum infected female cat: A case report

International Journal of Chemical Studies, 2019

A female cat aged 2 year was presented to the Referral Veterinary Polyclinic, IVRI Izatnagar with... more A female cat aged 2 year was presented to the Referral Veterinary Polyclinic, IVRI Izatnagar with complaint of continuous crying, weakness, inappetence, discomfort and anal rubbing. Physical examination of cat revealed flea infestation and faecal examination revealed the presence of multiple egg packets containing gravid segments. The systematic investigation showed reduced haemoglobin concentration and total erythrocyte count, but no significant changes in normal biochemical parameters. The institution of treatment to the cat with praziquantel along with the supportive therapy brought successful recovery in 7 days.

Typical Carcinoid: Cytological Diagnosis of a Case and Mimickers in Cytological Sampling Techniques with Brief Review of Literature

Annals of Pathology and Laboratory Medicine, 2019

Typical carcinoids are the most well differentiated and least aggressive of pulmonary neuroendocr... more Typical carcinoids are the most well differentiated and least aggressive of pulmonary neuroendocrine tumors. Cytologic diagnosis on trans bronchial needle aspirate (TBNA) smears is quite diagnostic; however, may be challenging on other cytologic specimens like bronchial brushings and bronchial washings. We discuss such a case of typical carcinoid in a 30 years old female.

Therapeutic Management of Ivermectin Toxicity in a Calf: A Case Report

International Journal of Current Microbiology and Applied Sciences, 2018

Localized laryngotracheobronchial amyloidosis: Management issues

Lung India, 2019

Pulmonary amyloidosis can have four different manifestations: diffuse parenchymal amyloidosis, no... more Pulmonary amyloidosis can have four different manifestations: diffuse parenchymal amyloidosis, nodular parenchymal amyloidosis, infiltrative tracheobronchial amylo idos i s , and nodu la r t r acheobronch ia l amyloidosis.[1] Our patient had localized nodular tracheobronchial amyloidosis associated with bilateral laryngeal nodular amyloid deposits on false vocal cords. Diffuse nodularity and thickening in the tracheobronchial tree along with areas of bronchial narrowing may result in complications such as atelectasis and postobstructive pneumonia.

Pulmonary Hamartoma Mimicking Malignancy: A Cytopathological Diagnosis

JOURNAL OF CLINICAL AND DIAGNOSTIC RESEARCH, 2016

A 53-year-old male presented to medicine outpatient department with complaints of cough with sput... more A 53-year-old male presented to medicine outpatient department with complaints of cough with sputum for last four months. The patient gave a history of chronic smoking and change in voice. However, there was no history of fever, dyspnea, haemoptysis, dysphagia, loss of appetite and weight loss. On general examination, there was absence of pallor, cyanosis, clubbing, oedema and any peripheral lymphadenopathy. On examination of the respiratory system mild left sided ronchi was noted on auscultation, however, bilateral air entry was normal. Rest of the systems examined did not reveal any abnormality.

Extracorporeal treatment with high-volume continuous venovenous hemodiafiltration and charcoal-based sorbent hemoperfusion for severe metformin-associated lactic acidosis

Indian Journal of Critical Care Medicine, 2016

We present a case of a 49-year-old female with an alleged history of ingestion of approximately 1... more We present a case of a 49-year-old female with an alleged history of ingestion of approximately 100 tablets of metformin (850 mg each). Investigations revealed severe lactic acidosis with lactate levels of 13.5 mmol/L and pH of 7.17. This indicates severe toxicity and is associated with a high mortality. Charcoal-based sorbent hemoperfusion was done as a desperate effort, as patient continued to deteriorate despite supportive care and high-volume continuous venovenous hemodiafiltration. The patient survived despite metformin-associated lactic acidosis related to severe metformin toxicity.

Spontaneous Expulsion of a Hydatid Cyst During Flexible Bronchoscopy

Journal of Bronchology, 2006

Wolters Kluwer Health may email you for journal alerts and information, but is committed to maint... more

ChemInform Abstract: Absolute Configuration of Calogenin (I) and Its 20-Keto Derivative (II)

ChemInform, 2009

The absolute configuration of calogenin (isolated from Periploca calophylla) and its 20-keto deri... more The absolute configuration of calogenin (isolated from Periploca calophylla) and its 20-keto derivative (isolated from Hemedesmus indicus) has been established as pregn-5-ene-3β,14β,20R triol containing a C-17 hydroxy ethyl chain in αconfiguration and pregn-5-ene-20-keto-3β,14β-diol containing a C-17 methyl keto chain in β-configuration, respectively.

C21 steroidal glycosides from Hemidesmus indicus

Phytochemistry, 2000

Two novel pregnane glycosides, denicunine (1) and heminine (4), have been isolated from the dried... more Two novel pregnane glycosides, denicunine (1) and heminine (4), have been isolated from the dried stem of Hemidesmus indicus R.Br. (family: Asclepiadaceae). Chemical transformations and spectroscopic evidence viz: 1H and 13C NMR spectroscopy and FABMS are consistent with the structures calogenin 3-O-3-O-methyl-beta-D-fucopyranosyl-(1--&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;gt;4)-O-beta-D-oleandropyranosi de and calogenin 3-O-beta-D-cymaropyranosyl-(1--&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;gt;4)-O-beta-D-digitoxopyranoside+ ++, respectively.

Two pregnane glycosides from Hemidesmus indicus

Phytochemistry, 1991

... 9422/91 $300+000 >( 1990 Pergamon Press pic TWO PREGNANE GLYCOSIDES FROM HEMIDESMUS INDICU... more

Indicusin-A Pregnane Diester Triglycoside from Hemidesmus indicus R. Br

Natural Product Letters, 1995

A novel pregnane oligoglycoside viz. Indicusin (1) was isolated from CHCl3-EtOH (3:2) fraction of... more A novel pregnane oligoglycoside viz. Indicusin (1) was isolated from CHCl3-EtOH (3:2) fraction of Hemidesmus indicus R Br. (family Asclepiadaceae). With the help of FABMS, H NMR, C NMR spectroscopy and chemical transformations, the structure of 1 was defined as 11α, 12β-di-O-acetyl-orgogenin-3-O-β-D-cymaropyranosyl (1→4)-O-β-D-cymaropyranosyl (1→4)-O-β-D-cymaropyranoside.

Clinical profile, intensive care unit course, and outcome of patients admitted in intensive care unit with dengue

Journal of Critical Care, 2011

The purpose of the study was to assess the clinical profile and course of dengue patients admitte... more The purpose of the study was to assess the clinical profile and course of dengue patients admitted to the intensive care unit (ICU) and to identify factors related to poor outcome. All patients with dengue admitted to ICU over 2.5 years were included prospectively. Severity of illness was assessed by the Acute Physiology and Chronic Health Evaluation (APACHE) II score, and organ failure was determined by the Sequential Organ Failure Assessment score. Primary outcome measure was 28-day mortality. Logistic regression analysis was performed to identify factors predicting mortality. Data from 198 patients were analyzed. Mean age was 39.56 ± 17.1 years, and 61.1% were male. The commonest complaints were fever (96%) and rash (37.9%). Mean admission APACHE II and Sequential Organ Failure Assessment scores were 7.52 ± 7.8 and 4.52 ± 3.4, respectively. The commonest organ failure was coagulation (43.4%) followed by respiratory failure (13.1%). Vasopressors were required by 11.6%; and dialysis and mechanical ventilation were required by 7.6% and 9.1%, respectively. Mortality was 12 (6.1%); and on multivariate analysis, APACHE II score (odds ratio, 1.781; 95% confidence interval, 0.967-3.281; P = .048) could independently predict mortality. Patients with dengue fever may require ICU admission for organ failure. Outcome is good if appropriate aggressive care and organ support are instituted. Admission APACHE II score may predict patients at higher risk of death.

Correlation of oxidative status with BMI and lung function in COPD

Clinical Biochemistry, 2007

Objectives: The imbalance in oxidative status together with nutrition depletion and low body weig... more Objectives: The imbalance in oxidative status together with nutrition depletion and low body weight play a vital role in the pathogenesis and severity of chronic obstructive pulmonary disease (COPD). The study was undertaken to ascertain if a relationship existed between oxidative status and BMI in COPD. In addition, association of oxidative status and BMI with lung function of the disease was also examined. Materials and methods: In 202 COPD patients and 136 healthy controls plasma lipid peroxidation (LPO), reduced glutathione (GSH), glutathione peroxidase (GPx), catalase (CAT) activities, BMI and FEV 1 % predicted were looked for interactions. Results: The patients had increased LPO (p = 0.006) and decreased antioxidants (GSH, p = 0.005; GPx, p = 0.035 and CAT, p = 0.008, respectively). Of note are the correlations of oxidative stress markers with BMI and FEV 1 % predicted in the patients. LPO inversely and GSH, GPx, and CAT positively correlated with both BMI (p = 0.007, p b 0.001, p = 0.045 and p = 0.009, respectively), and FEV 1 % of predicted (LPO, p = 0.001; GSH, p b 0.001; GPx, p = 0.043 and CAT, p b 0.001) in the patients. Further, a positive correlation existed between BMI and FEV 1 % predicted (p = 0.016) in COPD. Conclusion: The intimate relationship of oxidative status with BMI and lung function, and the direct correlation between BMI and FEV 1 may potentiate severity of the disease.

CYP1A1, CYP1A2 and CYBA gene polymorphisms associated with oxidative stress in COPD

Clinica Chimica Acta, 2010

Background: The genetic susceptibility to chronic obstructive pulmonary disease (COPD) depends on... more Background: The genetic susceptibility to chronic obstructive pulmonary disease (COPD) depends on detoxification and antioxidant enzymes, which detoxify cigarette smoke reactive components that, otherwise, generate oxidative stress. Methods: In a case-control study of 346 subjects with and without COPD, we examined the polymorphisms 462Ile/Val, 3801T/C of CYP1A1, −3860G/A of CYP1A2 and −930A/G, 242C/T of CYBA individually or in combination and their contribution to oxidative stress markers by measuring malondialdehyde (MDA), catalase (CAT), glutathione (GSH) and glutathione peroxidase (GPx). Results: COPD patients had significantly increased MDA concentration (p < 0.001) and decreased CAT activity, GSH concentration, GPx activity (p ≤ 0.01). The patients were over-represented by the alleles 462Val, 3801C of CYP1A1 and −930G, 242C of CYBA (p < 0.001, p=0.003, p =0.030 and p = 0.031, respectively) and consequently the haplotypes of same alleles i.e. 462Val:3801C, 462Val:3801T and −930G:242C (p = 0.048, p = 0.016 and p =0.039, respectively). Similarly, CYP1A1 and CYP1A2 haplotypes, 462Val:3860G and 462Val:3801T:3860G were significantly over-represented (p = 0.001 and p =0.003), respectively in patients. The same alleles-associated genotype-combinations between genes were more prevalent in patients. Of note, the genotypes, 462Ile/Val+Val/ Val, 3801TC+CC of CYP1A1 and −930AG+GG of CYBA associated with increased MDA concentration (p = 0.018, p = 0.045 and p =0.017, respectively), decreased CAT activity (p < 0.0001, p =0.080 and p < 0.0001, respectively) and GSH concentration (p < 0.0001, p = 0.0002 and p =0.011, respectively) in patients. Conclusion: The identified alleles, its haplotypes and the genotype-combination along with increased oxidative stress, signify the importance in susceptibility to COPD.

Genetic polymorphisms of GSTP1 and mEPHX correlate with oxidative stress markers and lung function in COPD

Biochemical and Biophysical Research Communications, 2007

The genetic susceptibility to COPD might depend on variations in detoxification enzymes that acti... more The genetic susceptibility to COPD might depend on variations in detoxification enzymes that activate and detoxify cigarette smoke products, which otherwise generate oxidative stress causing pathogenesis. In a case-control study of 202 COPD patients and 136 normals, we examined the association of polymorphisms I105V, A114V of GSTP1 and Y113H, H139R of mEPHX individually or in combination with disease and their contribution to oxidative stress markers such as MDA, GSH, GPx and airflow obstruction. Patients were over-represented by the alleles 105V, 114V of GSTP1 and 113H, 139H of mEPHX (v 2 = 10.63, p = 0.001, v 2 = 13.92, p < 0.001, v 2 = 13.02, p < 0.001 and v 2 = 4.48, p = 0.034, respectively) and the haplotypes of same alleles i.e. 105V-114V and 113H-139H (v 2 = 14.58, p < 0.001 and v 2 = 23.14, p < 0.001). Moreover, there was marked over-representation of combination of genotypes, I105I+A114A of GSTP1 (53% vs. 36%) in controls; whereas, the combinations with 105V/114V alleles (64% vs. 47%) of GSTP1 (OR = 1.99; 95% CI = 1.28-3.09; p = 0.002) and the homozygotes H113H+H139H (27% vs.10%) of mEPHX (OR = 3.26; 95% CI = 1.73-6.15; p = 0.0001) in patients. Patients had significantly elevated MDA level (p < 0.001) and decreased GSH level (p < 0.001) and GPx activity (p = 0.035), respectively. Of note, the genotypes, I105V/V105V, A114V/V114V of GSTP1 and Y113H/ H113H of mEPHX associated with increased MDA level (p = 0.04, p = 0.03 and p = 0.003), decreased GSH level (p = 0.019, p = 0.007 and p = 0.0006) and lower FEV1 (p = 0.23, p = 0.037 and p = 0.029), respectively, in patients; so was the correlation of these biomarkers and lung function with the combinations of the genotypes. In conclusion, 105V/114V alleles of GSTP1 and 113H/139H alleles of mEPHX and the combination of genotypes with same alleles associated with imbalanced oxidative stress and lung function in patients, signifying the importance in the disease.

$Research paper thumbnail of A novel pentasaccharide from immunostimulant oligosaccharide fraction of buffalo milk$

A novel pentasaccharide from immunostimulant oligosaccharide fraction of buffalo milk

Biochimica et Biophysica Acta (BBA) - General Subjects, 1999

A processed oligosaccharide mixture of buffalo milk induced significant stimulation of antibody, ... more A processed oligosaccharide mixture of buffalo milk induced significant stimulation of antibody, delayed-type hypersensitivity response to sheep red blood cells in BALB/c mice. This also stimulated non-specific immune response of the animals measured in terms of macrophage migration index. A novel pentasaccharide has been isolated from the oligosaccharide containing fraction having immunostimulant activity of buffalo milk. This compound was isolated by a combination of gel filtration chromatography, silica gel column chromatography of derivatised oligosaccharides while the homogeneity was confirmed by high performance liquid chromatography. The results of structural analyses, i.e. proton nuclear magnetic resonance, fast atom bombardment mass spectrometry, chemical transformations and degradations are consistent with the following structure : GlcNAcL1 3 3GalL1 3 4GlcNAcL1 3 3GalL1 3 4Glc

Reply by Authors to P. J. Paul

AIAA Journal, 1977

Heterogeneous Combustion of Aluminized Propellants

AIAA Journal, 1976

Hybrid combustion of an aluminized polystyrene/oxygen system has been investigated. The mass cons... more Hybrid combustion of an aluminized polystyrene/oxygen system has been investigated. The mass consumption rate of the fuel is found to be proportional to the square root of the mass consumption rate of the oxidizer. The regression rate depends on time, as was found to be the case for an unmetallized propellant. The role of radiative heat transfer to the combustion products and unreacted oxidizer is confirmed when the data are analyzed in the context of the theory of Marxman et al. Analysis of the data takes into account the heat taken away by metal particles during combustion. This is found to be strongly dependent on the oxidizer flow rate.

Endothermic fuels for supersonic ramjet

Zenodo (CERN European Organization for Nuclear Research), 2003

Greater ease of use and higher density make hydrocarbon fuels an attractive option for hypersonic... more Greater ease of use and higher density make hydrocarbon fuels an attractive option for hypersonic space transport vehicle. In addition to use of fuel for propulsive purpose, it is used for regenerative cooling of system structures. In order to achieve maximum heat sink, endothermic reactions of the hydrocarbon fuels must be employed to augment fuel enthalpy. This should provide cooling sufficient to allow flight in the range of Mach 4-10 at optimum altitude. For a hydrocarbon fuel blend to be suitable for the hypersonic vehicle it should selectively pursue the most beneficial endothermic reaction for optimum cooling and the reaction products should have high combustion intensity under ramjet and scramjet engine conditions. The problem of formation of deposits/coke by hydrocarbons at higher temperature needs to be resolved. The issues for the development of hydrocarbon fuel for hypersonic vehicle are highlighted in this paper.