Papers by Delia Cruz-vega
Review Article Liver Fibrosis and Protection Mechanisms Action of Medicinal Plants Targeting Apoptosis of Hepatocytes and
which permits unrestricted use, distribution, and reproduction in any medium, provided the origin... more which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Following chronic liver injury, hepatocytes undergo apoptosis leading to activation of hepatic stellate cells (HSC). Consequently, activated HSC proliferate and produce excessive extracellular matrix, responsible for the scar formation. The pandemic trend of obesity, combined with the high incidence of alcohol intake and viral hepatitis infections, highlights the urgent need to find accessible antifibrotic therapies. Treatment strategies should take into account the versatility of its pathogenesis and act on all the cell lines involved to reduce liver fibrosis. Medicinal plants are achieving popularity as antifibrotic agents, supported by their safety, cost-effectiveness, and versatility.This review will describe the role of hepatocytes and HSC in the pathogenesis of liver fibrosis and detail the mechanisms of modulation of apoptosis of both cell lines by twelv...
Heliotropium amplexicaule es una planta perteneciente a la familia Boraginacea con antecedentes d... more Heliotropium amplexicaule es una planta perteneciente a la familia Boraginacea con antecedentes de contenido de compuestos con interes farmacologico. En este estudio se investigo la actividad biologica de los extractos metanolicos de raiz, tallo, flor y hojas de H. amplexicaule sobre diferentes microorganismos. Los resultados mostraron un efecto amebicida significativo de los extractos de raiz, tallo, flor y hojas sobre Entamoeba histolytica con CI50 de 2, 8, 36 y 114.2 μg/mL respectivamente; no mostraron actividad sobre las bacterias y dermatofitos probados. La citotoxicidad obtenida para raiz, tallo, hoja y flor sobre la linea celular Vero fue CI50 de: 250, 200, 175 y 150 μg/mL respectivamente; y la toxicidad para todos los extractos sobre A. salina fue de LD50 > 1,000 μg/mL
Regenerative Medicine, 2017
The occurrence of chronic diseases such as neurological, metabolic and cardiovascular degenerativ... more The occurrence of chronic diseases such as neurological, metabolic and cardiovascular degenerative disorders increases with age. Cell therapy is an emerging approach to the treatment of these conditions. Of particular interest is the application of autologous stem cells because it eliminates post-transplantation immune rejection and there are less ethical concerns associated with their use. The regenerative capacity of stem cells harvested from elderly people is however controversial. In this review, we analyze if self-renewal potential, differentiation capability and expression of stemness genes in stem cells collected from elderly patients validate their application in clinical trials and examine the results.
International journal of stem cells, Jan 31, 2016
Diabetes constitutes a worldwide epidemic that affects all ethnic groups. Cell therapy is one of ... more Diabetes constitutes a worldwide epidemic that affects all ethnic groups. Cell therapy is one of the best alternatives of treatment, by providing an effective way to regenerate insulin-producing cells lost during the course of the disease, but many issues remain to be solved. Several groups have been working in the development of a protocol capable of differentiating Mesenchymal Stem Cells (MSCs) into physiologically sound Insulin Producing Cells (IPCs). In order to obtain a simple, fast and direct method, we propose in this manuscript the induction of MSCs to express NESTIN in a short time period (2 h), proceeded by incubation in a low glucose induced medium (24 h) and lastly by incubation in a high glucose medium. Samples from cell cultures incubated in high glucose medium from 12 to 168 h were obtained to detect the expression of INSULIN-1, INSULIN -2, PDX-1 and GLUT-2 genes. Induced cells were exposed to a glucose challenge, in order to assess the production of insulin. This met...
International Journal of Inflammation, 2015
Inflammation is a central feature of liver fibrosis as suggested by its role in the activation of... more Inflammation is a central feature of liver fibrosis as suggested by its role in the activation of hepatic stellate cells leading to extracellular matrix deposition. During liver injury, inflammatory cells are recruited in the injurious site through chemokines attraction. Thus, inflammation could be a target to reduce liver fibrosis. The pandemic trend of obesity, combined with the high incidence of alcohol intake and viral hepatitis infections, highlights the urgent need to find accessible antifibrotic therapies. Medicinal plants are achieving popularity as antifibrotic agents, supported by their safety, cost-effectiveness, and versatility. The aim of this review is to describe the role of inflammation and the immune response in the pathogenesis of liver fibrosis and detail the mechanisms of inhibition of both events by medicinal plants in order to reduce liver fibrosis.
Chinese Medicine, 2014
During chronic liver injury, hepatic stellate cells (HSC) are activated and proliferate, which ca... more During chronic liver injury, hepatic stellate cells (HSC) are activated and proliferate, which causes excessive extracellular matrix (ECM) deposition, leading to scar formation and fibrosis. Medicinal plants are gaining popularity as antifibrotic agents, and are often safe, cost-effective, and versatile. This review aims to describe the protective role and mechanisms of medicinal plants in the inhibition of HSC activation and ECM deposition during the pathogenesis of liver fibrosis. A systematic literature review on the anti-fibrotic mechanisms of hepatoprotective plants was performed in PubMed, which yielded articles about twelve relevant plants. Many of these plants act via disruption of the transforming growth factor beta 1 signaling pathway, possibly through reduction in oxidative stress. This reduction could explain the inhibition of HSC activation and reduction in ECM deposition. Medicinal plants could be a source of anti-liver fibrosis compounds.
Stem Cells International, 2013
Adipose-derived stem cells (ADSCs) are a heterogeneous cell population that may be enriched by po... more Adipose-derived stem cells (ADSCs) are a heterogeneous cell population that may be enriched by positive selection with antibodies against the low-affinity nerve growth factor receptor (LNGFR or CD271), yielding a selective cell universe with higher proliferation and differentiation potential. This paper addresses the need for determining the quantity of ADSCs positive for the CD271 receptor and its correlation with donor's age. Mononuclear cells were harvested from the lower backs of 35 female donors and purified using magnetic beads. Multipotency capacity was tested by the expression of stemness genes and through differentiation into preosteoblasts and adipocytes. A significant statistical difference was found in CD271+concentrations between defined age intervals. The highest yield was found within women on the 30–40-year-old age range. CD271+ADSCs from all age groups showed differentiation capabilities as well as expression of typical multipotent stem cell genes. Our data sugg...
Culture media formulation has a direct influence on growth, morphology and physiology of cells. I... more Culture media formulation has a direct influence on growth, morphology and physiology of cells. In this work, a mammalian cell culture medium was formulated based on peptone and yeast extract with an industrial reagent level. Three different mammalian cell lines, OK, CHANG y LLCPK-1 were successfully grown in a 25:75 mix of MEM:MD (MEM = control medium; MD = house
Antimycobacterial activity of Juglans regia, Juglans mollis, Carya illinoensis and Bocconia frutescens
Phytotherapy Research, 2008
Mycobacterium tuberculosis is a serious worldwide health threat, killing almost 2 million people ... more Mycobacterium tuberculosis is a serious worldwide health threat, killing almost 2 million people per year. Alternative antimycobacterial drugs are urgently needed; studies have shown that medicinal plants traditionally used to treat respiratory diseases are a potential source of compounds to treat tuberculosis. This paper studied the antimycobacterial activity of 28 extracts from four different plant species that have been used in traditional Mexican medicine to treat tuberculosis. Bark and leaf crude extracts of Juglans regia L., Juglans mollis Engelm., Carya illinoensis (Wangenh) K. Koch and Bocconia frutescens showed in vitro anti-M. tuberculosis activity. Hexane bark extracts from C. illinoensis, J. mollis and J. regia were the most active with a minimal inhibitory concentration (MIC) of 31, 50 and 100 microg/mL, respectively. Ethanol bark extracts from C. illinoensis and J. mollis showed activity at 100 and 125 microg/mL, respectively. Leaf extracts had the lowest activity. Methanol and hexane leaves extracts from B. frutescens had a MIC of 125 microg/mL. None of the aqueous extracts showed antimycobacterial activity.
Journal of Medical Microbiology, 2007
Several techniques have been used to quantify the cytotoxicity produced by Mycobacterium tubercul... more Several techniques have been used to quantify the cytotoxicity produced by Mycobacterium tuberculosis bacilli on cell monolayers; however, they are semi-quantitative or time consuming. Herein, a method based on crystal violet (CV) uptake by THP-1 cell monolayers is described. This colorimetric method quantifies the cytotoxic effect as a function of the number of remaining cells after the infection with M. tuberculosis. Since this micro-organism is not stained by the dye, it does not produce a background that affects absorbance readings. As determined by CV assay (CVA), M. tuberculosis strain H37Rv destroyed 10.5 % of THP-1 cell monolayers at 24 h and 50.52 % at 72 h, while M. tuberculosis strains lacking the complete phospholipase C locus produced a reduced cytotoxic effect. The damage estimated by microscopy corresponded to the effect quantified by CVA. The results show that the use of CVA is a rapid, sensitive and reliable quantitative assay to measure the cytotoxicity of differen...
Micropropagation of Lepidium virginicum (Brassicaceae), a plant with antiprotozoal activity
In Vitro Cellular & Developmental Biology - Plant, 2006
... LIDIA OSUNA1,2*, MARÍA ESTHER TAPIA-PÉ REZ1,3, ODETTE FIGUEROA1, ENRIQUE JIMÉ NEZ-FERRER1, MA... more ... LIDIA OSUNA1,2*, MARÍA ESTHER TAPIA-PÉ REZ1,3, ODETTE FIGUEROA1, ENRIQUE JIMÉ NEZ-FERRER1, MARÍA LUISA GARDUN O ... Ibanez, MR; Amo-Marco, JB Promotion by phloroglucinol of micropropagation of Minuartia valentine, an endangered and endemic ...
Experimental Parasitology, 2000
suggest that the seriousness of the amoebic infection depends on the host's susceptibility and on... more suggest that the seriousness of the amoebic infection depends on the host's susceptibility and on virulence factors of the parasite. Some host Diminution of erythrophagocytosis, phospholipase A 2 , and hemolytic activities is related to virulence impairment in long-term axenic cul-properties that favor invasive amoebiasis are intestinal microflora, certain nutritional habits, deficient immune response (Ackers 1996), tures. Experimental Parasitology 96, 116-119.
Advances in Pharmacological Sciences, 2014
Following chronic liver injury, hepatocytes undergo apoptosis leading to activation of hepatic st... more Following chronic liver injury, hepatocytes undergo apoptosis leading to activation of hepatic stellate cells (HSC). Consequently, activated HSC proliferate and produce excessive extracellular matrix, responsible for the scar formation. The pandemic trend of obesity, combined with the high incidence of alcohol intake and viral hepatitis infections, highlights the urgent need to find accessible antifibrotic therapies. Treatment strategies should take into account the versatility of its pathogenesis and act on all the cell lines involved to reduce liver fibrosis. Medicinal plants are achieving popularity as antifibrotic agents, supported by their safety, cost-effectiveness, and versatility. This review will describe the role of hepatocytes and HSC in the pathogenesis of liver fibrosis and detail the mechanisms of modulation of apoptosis of both cell lines by twelve known hepatoprotective plants in order to reduce liver fibrosis.
Comparing stemness gene expression between stem cell subpopulations from peripheral blood and adipose tissue
American journal of stem cells, 2018
Cell therapy presents a promising alternative for the treatment of degenerative diseases. The mai... more Cell therapy presents a promising alternative for the treatment of degenerative diseases. The main sources of adult stem cells are bone marrow, adipose tissue and peripheral blood. Within those tissues, there are cell subpopulations that share pluripotential characteristics. Nevertheless, there is insufficient data to determine which of these stem cell subtypes would have a better possibility to differentiate to a specific tissue. The objective of this research was to analyze and compare the stemness genes expression from peripheral blood and adipose tissue of plastic adherent cells, and those immune-selected by the CD133 and CD271 membrane markers. On all cell subpopulation groups, self-renew capacity, the membranes markers CD73, CD90 and CD105, as well as the stemness genes and, expression were analyzed. Results showed that all samples presented the minimal criteria to define them as human stem cells. All cell subpopulation were capable of self-renewal. Nevertheless, the subpopula...
Disease markers, 2018
Amyotrophic lateral sclerosis (ALS) is an aggressive neurodegenerative disorder that selectively ... more Amyotrophic lateral sclerosis (ALS) is an aggressive neurodegenerative disorder that selectively attacks motor neurons in the brain and spinal cord. Despite important advances in the knowledge of the etiology and progression of the disease, there are still no solid grounds in which a clinician could make an early objective and reliable diagnosis from which patients could benefit. Diagnosis is difficult and basically made by clinical rating scales (ALSRs and El Escorial). The possible finding of biomarkers to aid in the early diagnosis and rate of disease progression could serve for future innovative therapeutic approaches. Recently, it has been suggested that ALS has an important immune component that could represent either the cause or the consequence of the disease. In this report, we analyzed 19 different cytokines and growth factors in the cerebrospinal fluid of 77 ALS patients and 13 controls by decision tree and PanelomiX program. Results showed an increase of Adipsin, MIP-1b,...
Antiamebic Activity of Aqueous Extracts from Five Medicinal Plants from Northeastern Mexico
Archives of Medical Research, 2000
Archives of Medical Research, Volume 31, Issue 4, Pages S17-S18, July 2000, Authors:Delia E Cruz-... more Archives of Medical Research, Volume 31, Issue 4, Pages S17-S18, July 2000, Authors:Delia E Cruz-Vega; Abigail Aguilar; Jorge Castro-Garza; Ma.Teresa González-Garza.
Translational Research in Cancer [Working Title]
Chronic inflammation can trigger events that would induce the malignant transformation of cells a... more Chronic inflammation can trigger events that would induce the malignant transformation of cells and carcinogenesis. Cytokines play a crucial role and can control the development and multiplication of cancerous cells. However, clinical data present controversy about the participation of these proteins in the establishment and development of cancer. Interleukin 10 (IL-10), a potent anti-inflammatory cytokine, has been the subject of multiple studies. Several studies have reported that IL-10 has pro-and antitumor effects. Elevated levels of IL-10 are associated with increased tumor growth with poor prognosis and drug resistance. However, this cytokine has both tumor-promoting and tumor-inhibiting properties. In vitro and in vivo studies report mechanisms by which IL-10 expression downregulates class I, which results in the control of the metastatic disease. IL-10 also inhibits tumorigenesis via downregulation of other cytokines. The variation observed could be the result of concentration ranges of this protein, genetic polymorphism, or both. The value obtained may serve as a biomarker indicative of tumor development and its prognosis.
Análisis fitoquímico y caracterización parcial de compuestos con actividad estimuladora sobre macrófagos y/o actividad antimicrobiana en extractos de raíz, tallo y hoja de Carlowrightia cordifolia A. Gray
Detection of Autoantibodies to Vascular Endothelial Growth Factor Receptor-3 in Bile Duct Ligated Rats and Correlations with a Panel of Traditional Markers of Liver Diseases
Disease Markers, 2016
Through chromatographic techniques, the presence of taxol was detected in the bark, needles, and ... more Through chromatographic techniques, the presence of taxol was detected in the bark, needles, and twigs of the Mexican Yew, Taxus globosa Sch. at levels comparable to that of other species (e.g. T. brevifolia, T. baccatta). The presence of a microtubule-stabilizing activity in the fractions obtained from the bark and foliage was also observed.
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Papers by Delia Cruz-vega