BackgroundMultisystem inflammatory syndrome in children (MIS-C) associated with being infected wi... more BackgroundMultisystem inflammatory syndrome in children (MIS-C) associated with being infected with coronavirus-19 (COVID-19) is a life-threatening condition resulting from cytokine storm, increased synthesis of reactive oxygen species (ROSs), and hyperinflammation occurring in genetically predisposed children following an infection with SARS-CoV-2.AimThe primary aims of our study were to identify changes in the activity of antioxidant enzymes in erythrocytes and total oxidative status in plasma after being treated with methylprednisolone (MP).MethodsA prospective cohort study of 67 children (56.7% male) under 18 with MIS-C being treated with MP was conducted at the Mother and Child Health Institute from January 2021 to April 2022. The impact of the therapy was assessed on the basis of the clinical condition, haematological and biochemical blood parameters, and echocardiographic findings.Results59.7% of patients presented cardiovascular (CV) manifestations, while myocardial dysfunct...
Hemodialysis (HD) removes nitrogenous waste products from patients? blood through a semipermeable... more Hemodialysis (HD) removes nitrogenous waste products from patients? blood through a semipermeable membrane along a concentration gradient. Near-infrared spectroscopy (NIRS) is an underexplored method of monitoring the concentrations of several molecules that reflect the efficacy of the HD process in dialysate samples. In this study, we aimed to evaluate NIRS as a technique for the non-invasive detection of uremic solutes by assessing the correlations between the spectrum of the spent dialysate and the serum levels of urea, creatinine, and uric acid. Blood and dialysate samples were taken from 35 patients on maintenance HD. The absorption spectrum of each dialysate sample was measured three times in the wavelength range of 700-1700 nm, resulting in a dataset with 315 spectra. The artificial neural network (ANN) learning technique was used to assess the correlations between the recorded NIR-absorbance spectra of the spent dialysate and serum levels of selected uremic toxins. Very good...
The study aimed to assess the oncologic and reproductive outcome of fertility-sparing treatment o... more The study aimed to assess the oncologic and reproductive outcome of fertility-sparing treatment of stage I borderline ovarian tumors (BOTs). A retrospective study of patients aged 18-40 years with stage I BOTs surgically treated during a 10-year period was conducted. In total, 52 patients (average age 32.7+/-5.9) were followed for 16 to 137 months after BOT diagnosis (mean 73.4 months). The overall survival rate was 100%. Recurrence was registered in 4 patients (7.7%). All patients underwent a unilateral salpingo-oophorectomy and all histologic findings corresponded with primary BOTs (3 serous; 1 endometrioid). Higher parity increased while the histological type and stage did not impact recurrence. Average recurrence-free survival was 36.2+/-23.6 months regardless of histological type and stage. Recurrence occurred during the first 3 postoperative years in 75% of cases. Out of 45 women treated with fertility-sparing surgery, 64.4% attempted pregnancy and the pregnancy success rate w...
Decellularized corneas offer a promising and sustainable source of replacement grafts, mimicking ... more Decellularized corneas offer a promising and sustainable source of replacement grafts, mimicking native tissue and reducing the risk of immune rejection post-transplantation. Despite great success in achieving acellular scaffolds, little consensus exists regarding the quality of the decellularized extracellular matrix (ECM). Metrics used to evaluate ECM performance are study-specific, subjective, and semi-quantitative. Thus, this work focused on developing a computational method to examine the effectiveness of corneal decellularization. We combined conventional semi-quantitative histological assessments and automated scaffold evaluations based on textual image analyses to assess decellularization efficiency. Our study highlights that it is possible to develop contemporary artificial intelligence (AI) models based on random forests and support vector machine algorithms, which can identify regions of interest in acellularized corneal stromal tissue with relatively high accuracy. These...
Background: In multisystem inflammatory syndrome in children (MIS-C) temporarily associated with ... more Background: In multisystem inflammatory syndrome in children (MIS-C) temporarily associated with coronavirus disease-19 (COVID-19), myocardial damage has been reported. Materials and Methods: A retrospective observational cohort study included children under 18 who had a myocardial injury related to COVID-19 treated in mother and child health institute from April 2020 to August 2020. Myocardial injury related to COVID-19 was manifested by elevated serum cardiac troponin and NT-proBNP with LV dysfunction, arrhythmias, and coronary arteries (CAs) dilatation or aneurysms. During the short-term follow-up, cardiac testing (electrocardiography, laboratory analysis, echocardiography, 24-h Holter monitoring, exercise stress test, and cardiac magnetic resonance) was performed. Results: Six male adolescents (14.7 ± 2.4 years) were included in the analysis (2/6 had MIS-C shock syndrome). All patients had elevated acute-phase reactants and NT-proBNP, whereas troponins were elevated in 5/6 patients. Echocardiography revealed left ventricular (LV) systolic dysfunction (EF 45.2 ± 6.9%); 2/6 had dilated CAs. IVIG was prescribed to all patients with MIS-C. Four patients required inotropic drug support. During hospitalization, a significant reduction of CRP, LDH, NT-proBNP, and D-dimer (P < 0.05) was registered. LV systolic function recovery was registered 3 days after applied therapy (P < 0.001). None of the patients developed dilated cardiomyopathy or CA aneurysms. Conclusions: With early recognition and adequate MIS-C therapy, children recovered entirely, maintained in the short-term follow-up period.
It has already been proved that magnesium has protective effects on neuronal and vascular system ... more It has already been proved that magnesium has protective effects on neuronal and vascular system as well. Therefore, a huge number of experiments on different animal models have been shown that magnesium may decrease the incidence of ischemic brain stroke. So, it is shown that significant neuroprotective effect of magnesium on different experimental models of focal cerebral ischemia in many laboratories, reduce infarct volume ratio between 25-61%. However, maximal neuroprotective effect of magnesium directly correlate with plasma concentration of magnesium in the brain. According to the results from different experimental studies and various clinical trials we could conclude that magnesium decrease the relative risk of cerebrovascular ischemic stroke. Magnesium could also have a major role in prevention of neurological diseases, predominantly ischemic stroke.
Background: Cardiovascular complications with myocarditis in multisystem inflammatory syndrome in... more Background: Cardiovascular complications with myocarditis in multisystem inflammatory syndrome in children (MIS-C) associated with severe acute respiratory syndrome coronavirus 2 infection have been reported, but the optimal therapeutic strategy remains unknown. Methods: A retrospective cohort study included 19 patients with acute left ventricular systolic dysfunction associated with MIS-C, average years of age 13.2 ± 3.8, treated from April 2020 to April 2021. Results: Treatment failure (TF) was observed in 8 patients (in the intravenous immunoglobulin [IVIG] group 7/10; in the corticosteroid [CS] group 1/9). The independent risk factor for TF was IVIG treatment (odds ratio [OR] 18.6, 95% confidence interval [CI] 1.6–222.93, P = 0.02). Patients initially treated with CS became afebrile during in-hospital day 1 (1.5, interquartile range [IQR] 1–2), while IVIG-treated patients became afebrile on in-hospital day 4 (IQR 2–4.25), after CS was added. The C-reactive protein (CRP) signific...
Objectives: Physical activity level decreases during young adulthood. As social media are nowaday... more Objectives: Physical activity level decreases during young adulthood. As social media are nowadays widely used and are included into many people's daily routines, the interventions on these websites have the possibilities to be integrated into those routines without becoming a burden. The aim of this study was to assess physical activity level among first-and fifth-year medical students and social media intervention with the aim to improve physical activity among them.
Ghrelin, an endogenous ligand for the growth hormone (GH) secretagogue receptor, was originally p... more Ghrelin, an endogenous ligand for the growth hormone (GH) secretagogue receptor, was originally purified from rat stomach; subsequently, ghrelin neurons were found in the arcuate nuclei of rats. Central effects of the peptide on GH release, however, remain to be clarified. The aim of the present study was to determine the morphologic features of GH-producing pituicytes and serum GH concentration after central administration of ghrelin. Five injections of rat ghrelin or phosphate-buffered saline (PBS; n = 10 rats/group) were given every 24 hrs (1 μg of ghrelin in 5 μl of PBS) into the lateral cerebral ventricle of male rats. Significant (P < 0.05) increases in absolute and relative pituitary weights occurred in ghrelin-treated rats versus controls (58% and 41%, respectively). Morphometric parameters (i.e., the volume of GH cells, volume of their nuclei, and volume density) all significantly (P < 0.05) increased by 17%, 18%, and 19%, respectively, in the ghrelin-treated group ve...
Nephrology nursing journal : journal of the American Nephrology Nurses' Association
Nurses who work in hemodialysis (HD) are considered highly susceptible to burnout due to their cl... more Nurses who work in hemodialysis (HD) are considered highly susceptible to burnout due to their close relationship with incurable patients and handling sophisticated machinery. A total of 210 nurses from 12 state-owned HD centers in the Republic of Serbia anonymously completed a background information questionnaire providingfactual data on nurses' sociodemographic characteristics and working conditions using the Maslach Burnout Inventory--Health Services Survey. Almost half of the nurses (42.9%) were experiencing burnout High emotional exhaustion, high depersonalization, and low level of personal accomplishment were present in 40.9%, 8.6%, and 31.3% of nurses, respectively. The number of children, involuntary choice of current position, and unwillingness to choose the same type of job again were significant predictors of burnout. Our population of nurses working in HD was more affected by emotional exhaustion than their colleagues in other countries, but maintained high level of ...
Journal of physiology and pharmacology : an official journal of the Polish Physiological Society, 2008
Ghrelin is 28-amino acid peptide, which is produced mainly in the stomach. Since plasma ghrelin a... more Ghrelin is 28-amino acid peptide, which is produced mainly in the stomach. Since plasma ghrelin are strictly dependent on food intake, this hormone has significant effects on appetite and energy balance. The aim of this investigation was to examine the effects of centrally applied ghrelin on feeding dynamism by measuring the approaches to food container and the amount of food and water intake within 2 hours immediately after ghrelin or PBS injections. Body weight was obtained daily, while ending retroperitoneal (RP) and epididymal (EPI) white adipose tissue (WAT) contents as well as blood levels of leptin and insulin were measured. Five injections of rat ghrelin or PBS (n = 8 per group) were administered once per day (1 microg = 0.15 nmol of ghrelin in 5 microL of PBS), into lateral cerebral ventricle (ICV) of free feeding adult male rats. Results showed that in the first and the second 30-min intervals number of approaches to food container were significantly increased already afte...
Recent studies have suggested that silver nanoparticles (AgNPs) may affect cell DNA structure in ... more Recent studies have suggested that silver nanoparticles (AgNPs) may affect cell DNA structure in in vitro conditions. In this paper, we present the results indicating that AgNPs change nuclear complexity properties in isolated human epithelial buccal cells in a time-dependent manner. Epithelial buccal cells were plated in special tissue culture chamber / slides and were kept at 37°C in an RPMI 1640 cell culture medium supplemented with L-glutamine. The cells were treated with colloidal silver nanoparticles suspended in RPMI 1640 medium at the concentration 15 mg L⁻¹. Digital micrographs of the cell nuclei in a sample of 30 cells were created at five different time steps: before the treatment (controls), immediately after the treatment, as well as 15 , 30 and 60 min after the treatment with AgNPs. For each nuclear structure, values of fractal dimension, lacunarity, circularity, as well as parameters of grey level co-occurrence matrix (GLCM) texture, were determined. The results indicate time-dependent reduction of structural complexity in the cell nuclei after the contact with AgNPs. These findings further suggest that AgNPs, at concentrations present in today&#39;s over-the-counter drug products, might have significant effects on the cell genetic material.
Ghrelin is an endogenous peptide potentially useful in therapy of anorexia and other age-related ... more Ghrelin is an endogenous peptide potentially useful in therapy of anorexia and other age-related metabolic disturbances. We evaluated the influence of age on the orexigenic and lipid metabolism-altering effects of ghrelin. Peripubertal, young, adult and middle-aged rats (1, 2, 7 and 12 months old, respectively) were treated with 5 daily intracerebroventricular injections of ghrelin (0.15 nmol) or saline (control). The food intake was measured daily before treatment, while white adipose tissue and serum/plasma samples for detection of lipid metabolites/hormones were collected at the end of the experiment. The values of cumulative food intake and body weight gain declined, while the white adipose tissue deposits and blood concentrations of triglycerides, cholesterol and free fatty acids all increased with age. Ghrelin significantly increased all parameters, but the stimulatory effects on body weight gain and food intake were more pronounced in peripubertal/young rats, while the increase in white adipose mass, triglyceride and low-density lipoprotein cholesterol levels was more noticeable in adult/middle-aged animals. The decrease in sensitivity to ghrelin-mediated stimulation of food intake in older animals could not be explained by alterations in ghrelin&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;#39;s ability to reduce anorexigenic hormones insulin and leptin. However, the higher responsiveness of aged rats to ghrelin-mediated increase in lipid metabolites was accompanied by an increase in adrenocorticotropic hormone and corticosterone levels. These results indicate that aging, while reducing sensitivity to ghrelin-mediated increase in body weight gain and food intake, might enhance the responsiveness to the stimulatory effects of ghrelin on lipid metabolites and hypothalamic-pituitary-adrenal axis activity.
Cervix cancer occupies the third place after breast cancer and colon cancer white malignant tumor... more Cervix cancer occupies the third place after breast cancer and colon cancer white malignant tumors of the body uterus are in the fourth place. It is the most frequent tumor of female sexual organs. Aim of this study is to show the percentage of mortality of women caused ...
Ghrelin is a growth hormone secretagogue that plays an important role in appetite and weight regu... more Ghrelin is a growth hormone secretagogue that plays an important role in appetite and weight regulation. We have previously demonstrated that central ghrelin stimulated pituitary ACTH synthesis and secretion. In this study we further examined the effects of intracerebroventricular ...
... Milosevic 2 , Dejan Nesic 1 , Dragan Micic 3 , Mirjana Sumarac-Dumanovic 3 , Vera Popovic 3 &... more ... Milosevic 2 , Dejan Nesic 1 , Dragan Micic 3 , Mirjana Sumarac-Dumanovic 3 , Vera Popovic 3 &amp; Vesna Starcevic 1. 1 Institute of Medical Physiology, School of Medicine, University of Belgrade, Belgrade, Serbia; 2 Institute for Biological Research Sinisa Stankovic, University of ...
... 6, br. 1-2, str. 35-40. jezik rada: srpski saoptenje. Faktori koji određuju seksualno ponaa... more ... 6, br. 1-2, str. 35-40. jezik rada: srpski saoptenje. Faktori koji određuju seksualno ponaanje mladih. Neić Dejan a , Jović Stevan b , Nikolić Gordana c , Mrvaljević Milutin a , Lačković Maja a. ... VBS. Marić, J. (1998) Ljubav, seks i depresija - mali priručnik za srećniji ivot. ...
Medicinski glasnik Specijalne bolnice za bolesti štitaste žlezde i bolesti metabolizma, 2021
Continuous physical activity leads to a series of adaptive changes to athletes' cardiovascula... more Continuous physical activity leads to a series of adaptive changes to athletes' cardiovascular system, collectively called Athlete's heart. Recognizing such physiological ECG changes and distinguishing them from the pathological myocardium-related findings, play an important role in sudden cardiac death prevention among athletes. The aim of our study was to examine the effect of the type of sport on the frequency of electrocardiographic changes between two groups of subjects, football and volleyball players. The study included 47 subjects, students of the Faculty of Medicine, University of Belgrade, divided into two groups depending on type of sport they play. The first group consisted of 29 volleyball players, while the second group consisted of 18 football players. Anthropometric parameters: body height (BH), body weight (BW), body mass index (BMI), body fat percentage (%BF) were measured first. Resting electrocardiographic test was performed in supine position, using twel...
BackgroundMultisystem inflammatory syndrome in children (MIS-C) associated with being infected wi... more BackgroundMultisystem inflammatory syndrome in children (MIS-C) associated with being infected with coronavirus-19 (COVID-19) is a life-threatening condition resulting from cytokine storm, increased synthesis of reactive oxygen species (ROSs), and hyperinflammation occurring in genetically predisposed children following an infection with SARS-CoV-2.AimThe primary aims of our study were to identify changes in the activity of antioxidant enzymes in erythrocytes and total oxidative status in plasma after being treated with methylprednisolone (MP).MethodsA prospective cohort study of 67 children (56.7% male) under 18 with MIS-C being treated with MP was conducted at the Mother and Child Health Institute from January 2021 to April 2022. The impact of the therapy was assessed on the basis of the clinical condition, haematological and biochemical blood parameters, and echocardiographic findings.Results59.7% of patients presented cardiovascular (CV) manifestations, while myocardial dysfunct...
Hemodialysis (HD) removes nitrogenous waste products from patients? blood through a semipermeable... more Hemodialysis (HD) removes nitrogenous waste products from patients? blood through a semipermeable membrane along a concentration gradient. Near-infrared spectroscopy (NIRS) is an underexplored method of monitoring the concentrations of several molecules that reflect the efficacy of the HD process in dialysate samples. In this study, we aimed to evaluate NIRS as a technique for the non-invasive detection of uremic solutes by assessing the correlations between the spectrum of the spent dialysate and the serum levels of urea, creatinine, and uric acid. Blood and dialysate samples were taken from 35 patients on maintenance HD. The absorption spectrum of each dialysate sample was measured three times in the wavelength range of 700-1700 nm, resulting in a dataset with 315 spectra. The artificial neural network (ANN) learning technique was used to assess the correlations between the recorded NIR-absorbance spectra of the spent dialysate and serum levels of selected uremic toxins. Very good...
The study aimed to assess the oncologic and reproductive outcome of fertility-sparing treatment o... more The study aimed to assess the oncologic and reproductive outcome of fertility-sparing treatment of stage I borderline ovarian tumors (BOTs). A retrospective study of patients aged 18-40 years with stage I BOTs surgically treated during a 10-year period was conducted. In total, 52 patients (average age 32.7+/-5.9) were followed for 16 to 137 months after BOT diagnosis (mean 73.4 months). The overall survival rate was 100%. Recurrence was registered in 4 patients (7.7%). All patients underwent a unilateral salpingo-oophorectomy and all histologic findings corresponded with primary BOTs (3 serous; 1 endometrioid). Higher parity increased while the histological type and stage did not impact recurrence. Average recurrence-free survival was 36.2+/-23.6 months regardless of histological type and stage. Recurrence occurred during the first 3 postoperative years in 75% of cases. Out of 45 women treated with fertility-sparing surgery, 64.4% attempted pregnancy and the pregnancy success rate w...
Decellularized corneas offer a promising and sustainable source of replacement grafts, mimicking ... more Decellularized corneas offer a promising and sustainable source of replacement grafts, mimicking native tissue and reducing the risk of immune rejection post-transplantation. Despite great success in achieving acellular scaffolds, little consensus exists regarding the quality of the decellularized extracellular matrix (ECM). Metrics used to evaluate ECM performance are study-specific, subjective, and semi-quantitative. Thus, this work focused on developing a computational method to examine the effectiveness of corneal decellularization. We combined conventional semi-quantitative histological assessments and automated scaffold evaluations based on textual image analyses to assess decellularization efficiency. Our study highlights that it is possible to develop contemporary artificial intelligence (AI) models based on random forests and support vector machine algorithms, which can identify regions of interest in acellularized corneal stromal tissue with relatively high accuracy. These...
Background: In multisystem inflammatory syndrome in children (MIS-C) temporarily associated with ... more Background: In multisystem inflammatory syndrome in children (MIS-C) temporarily associated with coronavirus disease-19 (COVID-19), myocardial damage has been reported. Materials and Methods: A retrospective observational cohort study included children under 18 who had a myocardial injury related to COVID-19 treated in mother and child health institute from April 2020 to August 2020. Myocardial injury related to COVID-19 was manifested by elevated serum cardiac troponin and NT-proBNP with LV dysfunction, arrhythmias, and coronary arteries (CAs) dilatation or aneurysms. During the short-term follow-up, cardiac testing (electrocardiography, laboratory analysis, echocardiography, 24-h Holter monitoring, exercise stress test, and cardiac magnetic resonance) was performed. Results: Six male adolescents (14.7 ± 2.4 years) were included in the analysis (2/6 had MIS-C shock syndrome). All patients had elevated acute-phase reactants and NT-proBNP, whereas troponins were elevated in 5/6 patients. Echocardiography revealed left ventricular (LV) systolic dysfunction (EF 45.2 ± 6.9%); 2/6 had dilated CAs. IVIG was prescribed to all patients with MIS-C. Four patients required inotropic drug support. During hospitalization, a significant reduction of CRP, LDH, NT-proBNP, and D-dimer (P < 0.05) was registered. LV systolic function recovery was registered 3 days after applied therapy (P < 0.001). None of the patients developed dilated cardiomyopathy or CA aneurysms. Conclusions: With early recognition and adequate MIS-C therapy, children recovered entirely, maintained in the short-term follow-up period.
It has already been proved that magnesium has protective effects on neuronal and vascular system ... more It has already been proved that magnesium has protective effects on neuronal and vascular system as well. Therefore, a huge number of experiments on different animal models have been shown that magnesium may decrease the incidence of ischemic brain stroke. So, it is shown that significant neuroprotective effect of magnesium on different experimental models of focal cerebral ischemia in many laboratories, reduce infarct volume ratio between 25-61%. However, maximal neuroprotective effect of magnesium directly correlate with plasma concentration of magnesium in the brain. According to the results from different experimental studies and various clinical trials we could conclude that magnesium decrease the relative risk of cerebrovascular ischemic stroke. Magnesium could also have a major role in prevention of neurological diseases, predominantly ischemic stroke.
Background: Cardiovascular complications with myocarditis in multisystem inflammatory syndrome in... more Background: Cardiovascular complications with myocarditis in multisystem inflammatory syndrome in children (MIS-C) associated with severe acute respiratory syndrome coronavirus 2 infection have been reported, but the optimal therapeutic strategy remains unknown. Methods: A retrospective cohort study included 19 patients with acute left ventricular systolic dysfunction associated with MIS-C, average years of age 13.2 ± 3.8, treated from April 2020 to April 2021. Results: Treatment failure (TF) was observed in 8 patients (in the intravenous immunoglobulin [IVIG] group 7/10; in the corticosteroid [CS] group 1/9). The independent risk factor for TF was IVIG treatment (odds ratio [OR] 18.6, 95% confidence interval [CI] 1.6–222.93, P = 0.02). Patients initially treated with CS became afebrile during in-hospital day 1 (1.5, interquartile range [IQR] 1–2), while IVIG-treated patients became afebrile on in-hospital day 4 (IQR 2–4.25), after CS was added. The C-reactive protein (CRP) signific...
Objectives: Physical activity level decreases during young adulthood. As social media are nowaday... more Objectives: Physical activity level decreases during young adulthood. As social media are nowadays widely used and are included into many people's daily routines, the interventions on these websites have the possibilities to be integrated into those routines without becoming a burden. The aim of this study was to assess physical activity level among first-and fifth-year medical students and social media intervention with the aim to improve physical activity among them.
Ghrelin, an endogenous ligand for the growth hormone (GH) secretagogue receptor, was originally p... more Ghrelin, an endogenous ligand for the growth hormone (GH) secretagogue receptor, was originally purified from rat stomach; subsequently, ghrelin neurons were found in the arcuate nuclei of rats. Central effects of the peptide on GH release, however, remain to be clarified. The aim of the present study was to determine the morphologic features of GH-producing pituicytes and serum GH concentration after central administration of ghrelin. Five injections of rat ghrelin or phosphate-buffered saline (PBS; n = 10 rats/group) were given every 24 hrs (1 μg of ghrelin in 5 μl of PBS) into the lateral cerebral ventricle of male rats. Significant (P < 0.05) increases in absolute and relative pituitary weights occurred in ghrelin-treated rats versus controls (58% and 41%, respectively). Morphometric parameters (i.e., the volume of GH cells, volume of their nuclei, and volume density) all significantly (P < 0.05) increased by 17%, 18%, and 19%, respectively, in the ghrelin-treated group ve...
Nephrology nursing journal : journal of the American Nephrology Nurses' Association
Nurses who work in hemodialysis (HD) are considered highly susceptible to burnout due to their cl... more Nurses who work in hemodialysis (HD) are considered highly susceptible to burnout due to their close relationship with incurable patients and handling sophisticated machinery. A total of 210 nurses from 12 state-owned HD centers in the Republic of Serbia anonymously completed a background information questionnaire providingfactual data on nurses' sociodemographic characteristics and working conditions using the Maslach Burnout Inventory--Health Services Survey. Almost half of the nurses (42.9%) were experiencing burnout High emotional exhaustion, high depersonalization, and low level of personal accomplishment were present in 40.9%, 8.6%, and 31.3% of nurses, respectively. The number of children, involuntary choice of current position, and unwillingness to choose the same type of job again were significant predictors of burnout. Our population of nurses working in HD was more affected by emotional exhaustion than their colleagues in other countries, but maintained high level of ...
Journal of physiology and pharmacology : an official journal of the Polish Physiological Society, 2008
Ghrelin is 28-amino acid peptide, which is produced mainly in the stomach. Since plasma ghrelin a... more Ghrelin is 28-amino acid peptide, which is produced mainly in the stomach. Since plasma ghrelin are strictly dependent on food intake, this hormone has significant effects on appetite and energy balance. The aim of this investigation was to examine the effects of centrally applied ghrelin on feeding dynamism by measuring the approaches to food container and the amount of food and water intake within 2 hours immediately after ghrelin or PBS injections. Body weight was obtained daily, while ending retroperitoneal (RP) and epididymal (EPI) white adipose tissue (WAT) contents as well as blood levels of leptin and insulin were measured. Five injections of rat ghrelin or PBS (n = 8 per group) were administered once per day (1 microg = 0.15 nmol of ghrelin in 5 microL of PBS), into lateral cerebral ventricle (ICV) of free feeding adult male rats. Results showed that in the first and the second 30-min intervals number of approaches to food container were significantly increased already afte...
Recent studies have suggested that silver nanoparticles (AgNPs) may affect cell DNA structure in ... more Recent studies have suggested that silver nanoparticles (AgNPs) may affect cell DNA structure in in vitro conditions. In this paper, we present the results indicating that AgNPs change nuclear complexity properties in isolated human epithelial buccal cells in a time-dependent manner. Epithelial buccal cells were plated in special tissue culture chamber / slides and were kept at 37°C in an RPMI 1640 cell culture medium supplemented with L-glutamine. The cells were treated with colloidal silver nanoparticles suspended in RPMI 1640 medium at the concentration 15 mg L⁻¹. Digital micrographs of the cell nuclei in a sample of 30 cells were created at five different time steps: before the treatment (controls), immediately after the treatment, as well as 15 , 30 and 60 min after the treatment with AgNPs. For each nuclear structure, values of fractal dimension, lacunarity, circularity, as well as parameters of grey level co-occurrence matrix (GLCM) texture, were determined. The results indicate time-dependent reduction of structural complexity in the cell nuclei after the contact with AgNPs. These findings further suggest that AgNPs, at concentrations present in today&#39;s over-the-counter drug products, might have significant effects on the cell genetic material.
Ghrelin is an endogenous peptide potentially useful in therapy of anorexia and other age-related ... more Ghrelin is an endogenous peptide potentially useful in therapy of anorexia and other age-related metabolic disturbances. We evaluated the influence of age on the orexigenic and lipid metabolism-altering effects of ghrelin. Peripubertal, young, adult and middle-aged rats (1, 2, 7 and 12 months old, respectively) were treated with 5 daily intracerebroventricular injections of ghrelin (0.15 nmol) or saline (control). The food intake was measured daily before treatment, while white adipose tissue and serum/plasma samples for detection of lipid metabolites/hormones were collected at the end of the experiment. The values of cumulative food intake and body weight gain declined, while the white adipose tissue deposits and blood concentrations of triglycerides, cholesterol and free fatty acids all increased with age. Ghrelin significantly increased all parameters, but the stimulatory effects on body weight gain and food intake were more pronounced in peripubertal/young rats, while the increase in white adipose mass, triglyceride and low-density lipoprotein cholesterol levels was more noticeable in adult/middle-aged animals. The decrease in sensitivity to ghrelin-mediated stimulation of food intake in older animals could not be explained by alterations in ghrelin&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;#39;s ability to reduce anorexigenic hormones insulin and leptin. However, the higher responsiveness of aged rats to ghrelin-mediated increase in lipid metabolites was accompanied by an increase in adrenocorticotropic hormone and corticosterone levels. These results indicate that aging, while reducing sensitivity to ghrelin-mediated increase in body weight gain and food intake, might enhance the responsiveness to the stimulatory effects of ghrelin on lipid metabolites and hypothalamic-pituitary-adrenal axis activity.
Cervix cancer occupies the third place after breast cancer and colon cancer white malignant tumor... more Cervix cancer occupies the third place after breast cancer and colon cancer white malignant tumors of the body uterus are in the fourth place. It is the most frequent tumor of female sexual organs. Aim of this study is to show the percentage of mortality of women caused ...
Ghrelin is a growth hormone secretagogue that plays an important role in appetite and weight regu... more Ghrelin is a growth hormone secretagogue that plays an important role in appetite and weight regulation. We have previously demonstrated that central ghrelin stimulated pituitary ACTH synthesis and secretion. In this study we further examined the effects of intracerebroventricular ...
... Milosevic 2 , Dejan Nesic 1 , Dragan Micic 3 , Mirjana Sumarac-Dumanovic 3 , Vera Popovic 3 &... more ... Milosevic 2 , Dejan Nesic 1 , Dragan Micic 3 , Mirjana Sumarac-Dumanovic 3 , Vera Popovic 3 &amp; Vesna Starcevic 1. 1 Institute of Medical Physiology, School of Medicine, University of Belgrade, Belgrade, Serbia; 2 Institute for Biological Research Sinisa Stankovic, University of ...
... 6, br. 1-2, str. 35-40. jezik rada: srpski saoptenje. Faktori koji određuju seksualno ponaa... more ... 6, br. 1-2, str. 35-40. jezik rada: srpski saoptenje. Faktori koji određuju seksualno ponaanje mladih. Neić Dejan a , Jović Stevan b , Nikolić Gordana c , Mrvaljević Milutin a , Lačković Maja a. ... VBS. Marić, J. (1998) Ljubav, seks i depresija - mali priručnik za srećniji ivot. ...
Medicinski glasnik Specijalne bolnice za bolesti štitaste žlezde i bolesti metabolizma, 2021
Continuous physical activity leads to a series of adaptive changes to athletes' cardiovascula... more Continuous physical activity leads to a series of adaptive changes to athletes' cardiovascular system, collectively called Athlete's heart. Recognizing such physiological ECG changes and distinguishing them from the pathological myocardium-related findings, play an important role in sudden cardiac death prevention among athletes. The aim of our study was to examine the effect of the type of sport on the frequency of electrocardiographic changes between two groups of subjects, football and volleyball players. The study included 47 subjects, students of the Faculty of Medicine, University of Belgrade, divided into two groups depending on type of sport they play. The first group consisted of 29 volleyball players, while the second group consisted of 18 football players. Anthropometric parameters: body height (BH), body weight (BW), body mass index (BMI), body fat percentage (%BF) were measured first. Resting electrocardiographic test was performed in supine position, using twel...
Uploads
Papers by Dejan Nesic