Papers by Deepmala Sharma
Pseudomonas aeruginosa quorum sensing molecule N-3-oxo-dodecanoyl-l-homoserine lactone activates human platelets through intracellular calcium-mediated ROS generation
International Journal of Medical Microbiology, Oct 1, 2018
Pseudomonas aeruginosa, an opportunistic pathogen release N-3-oxo-dodecanoyl-l-homoserine lactone... more Pseudomonas aeruginosa, an opportunistic pathogen release N-3-oxo-dodecanoyl-l-homoserine lactone (3-oxo-C12HSL) and N-butyryl-l-homoserine lactone (C4-HSL) quorum sensing (QS) molecules to regulate various virulence factors responsible for infection in the host. 3-oxo-C12 HSL not only regulates the bacterial gene expression but also modulates the host cell system. Thus, it is pertinent to evaluate the effect of these QS molecules on blood platelets which is responsible for the maintenance of hemostasis and thrombus formation. Here, in the present study, we showed that 3-oxo-C12 HSL activates platelets in a dose-dependent manner and induces intracellular calcium-mediated reactive oxygen species (ROS) release, whereas no such effect was observed with C4-HSL. 3-oxo-C12 HSL stimulated ROS release was mediated by NADPH oxidase. Results confirmed the involvement of phospholipase C (PLC) and IP3 receptor behind intracellular calcium-mediated ROS generation. The impact of 3-oxo-C12 HSL on platelet activation suggests that it could interfere and alter the normal function of platelet in individuals infected with P. aeruginosa.
Advances in data security through elliptical curve cryptography
Nucleation and Atmospheric Aerosols, 2023
Walsh-Hadamard spectrum is widely used in the field of science and technology like classification... more Walsh-Hadamard spectrum is widely used in the field of science and technology like classification of cancer cells, image processing, speech processing, signal and image compression etc. In this paper, a genomic analysis using Walsh-Hadamard spectrum and cross-correlation has been done. Transformation of genetic code using Walsh-Hadamard spectrum has been given. We redefine the Walsh-Hadamard spectrum in genomics and analyse the origin of mRNA features by using this spectra. Finally, using Walsh-Hadamard spectrum the overall energy of the mRNA sequence has been evaluated.
Journal of applied biology and biotechnology, Nov 10, 2021
Staphylococcus epidermidis and Pseudomonas aeruginosa are the two most commonly detected bacteria... more Staphylococcus epidermidis and Pseudomonas aeruginosa are the two most commonly detected bacteria. Pseudomonas aeruginosa exerts significant impacts on pathogenesis through quorum-sensing regulation, while S. epidermidis role has always remained a matter of interest due to its frequent presence in most of the hospitalized samples and even inside the body during operation procedures. In this study, the effect of S. epidermidis cells on P. aeruginosa pathogenesis was analyzed. The effect of S. epidermidis cells on P. aeruginosa growth was analyzed by using microtiter plate assay, Colony forming unit (CFU), and microscopy. The effect of virulence factors including protease, rhamnolipid, and swarming motility was also investigated. Escherichia coli pJN105LpSC11, a bioreporter strain, was used to analyze the effect of S. epidermidis on P. aeruginosa quorum-sensing. The growth of P. aeruginosa did not affect S. epidermidis cells of 5.5x10 9 , 6x10 9 , 7x10 9 , 8.5x10 9 , and 1.5x10 10 CFU/ml, and microscopy results are consistent with the findings. P. aeruginosaassociated virulence factors show that with increasing S. epidermidis counts, P. aeruginosa-associated virulence factors were reduced. No effect of S. epidermidis on P. aeruginosa quorum-sensing was observed. The outcomes suggest that S. epidermidis can be used as an alternative to reduce Pseudomonas aeruginosaassociated virulence factors and its pathogenesis.
On higher order nonlinearities of Boolean functions
Cryptography and Communications, May 30, 2023
Notes on Number Theory and Discrete Mathematics, Dec 1, 2018
In this article, we characterize generalized negabent functions on Z n 2 with values in Z 8 and Z... more In this article, we characterize generalized negabent functions on Z n 2 with values in Z 8 and Z 16. Furthermore, we propose several constructions of generalized negabent functions.
Senna alexandriana mill as a potential inhibitor for quorum sensing-controlled virulence factors and biofilm formation in Pseudomonas aeruginosa PAO1
Pharmacognosy Magazine, 2020
Background: Senna alexandriana Mill is a medicinally important plant used in laxatives and also p... more Background: Senna alexandriana Mill is a medicinally important plant used in laxatives and also possesses potent antimicrobial compounds, but its effect on the Pseudomonas aeruginosa was never analyzed. Aim: This study was carried out with the objective to attenuate quorum sensing (QS)-controlled virulence factors in P. aeruginosa and its biofilm. Methods: In the present study, acetone and hexane extracts of S. alexandriana mill were tested at sub-minimum inhibitory concentration concentrations for their anti-QS activity using bioreporter strain the Chromobacterium violaceum CV026. The effect of S. alexandriana Mill on different virulence phenotypes such as pyocyanin and protease production was investigated. Finally, the effect of the extract on P. aeruginosa, biofilm formation was also determined using the spectrophotometry-based method and microscopic analysis. Results: The extracts were tested for their activities against QS-controlled virulence factors of P. aeruginosa at 600 μg/ml and 800 μg/ml acetone and hexane extract concentration, respectively. Results showed that both the extracts have an inhibitory effect on different virulence factors of P. aeruginosa, including pyocyanin, protease production, and biofilm formation, without affecting the growth. The P. aeruginosa biofilm was found to be decreased by 75% and 62% in acetone and hexane extract presence. Conclusion: The results revealed that both acetone and hexane extracts showed anti-biofilm and anti-QS activity. It depicted the potential of S. alexandriana Mill against P. aeruginosa infection, its biofilm, and QS.
Pseudomonas aeruginosa quorum-sensing molecule N-(3-oxo-dodecanoyl)-l-homoserine lactone triggers mitochondrial dysfunction and apoptosis in neutrophils through calcium signaling
Medical Microbiology and Immunology, Aug 3, 2019
Pseudomonas aeruginosa is an opportunistic pathogen that utilizes the quorum-sensing (QS) process... more Pseudomonas aeruginosa is an opportunistic pathogen that utilizes the quorum-sensing (QS) process to regulate the production of different virulence factors and biofilm. N-3-oxo-dodecanoyl-L-homoserine lactone (C12) is a key QS molecule of P. aeruginosa which interacts with the mammalian immune cells and modulates their function. Here, we investigated the molecular mechanism of C12-induced apoptosis in neutrophils. Our data show that C12 causes apoptosis in neutrophils through an elevation in cytosolic and mitochondrial Ca2+ levels. Besides, C12 induces phosphatidylserine (PS) exposure, mitochondrial membrane potential (MMP) depolarization, mitochondrial permeability transition pore (MPTP) formation and mitochondrial reactive oxygen species (mROS) generation. C12-induced rise in intracellular Ca2+ level is majorly contributed by endoplasmic reticulum store through the activation of inositol 1, 4, 5-triphosphate receptor. Intracellular calcium chelation inhibited C12-induced mitochondrial dysfunction and apoptosis. Further, inhibition of mitochondrial Ca2+ uniporter by ruthenium red or Ru360 abrogated C12-induced mitochondrial Ca2+ uptake, MMP loss, MPTP opening, mROS production, and PS exposure. These mechanistic insights are expected to provide a better understanding of the role of C12 in P. aeruginosa pathogenesis.
Screening of anti-microbial, anti-biofilm activity, and cytotoxicity analysis of a designed polyherbal formulation against shigellosis
Journal of Ayurveda and Integrative Medicine, Oct 1, 2021
Background Shigellosis is an infectious intestinal disease common in rural communities. In develo... more Background Shigellosis is an infectious intestinal disease common in rural communities. In developing countries, shigellosis is caused predominantly by Shigella flexneri and has been determined as a major cause of morbidity and mortality. Objective The study investigates the anti-biofilm, anti-microbial, and anti-shigellosis activity of a designed formulation. Materials and methods The potential of the formulation against S. flexneri (MTCC 1457) was investigated using a well-diffusion assay. Further, the effect of the designed formulation on bacterial growth and biofilm formation was analyzed by the spectrophotometry method. Anti-quorum sensing activity was analyzed using Chromobacterium violaceum CV026, a bacterial strain. Finally, the cytotoxicity of the formulation was examined by using cell line and brine shrimp lethality assay. Results The MIC value of the aqueous extract of the formulation was 2.4 mg ml−1 and an inhibitory zone of 23 mm was observed. On the other side, the formulation significantly inhibited the bacterial growth, biofilm formation (23.78%), violacein inhibition (27.68%) at 0.6 mg ml−1 concentration (did not significantly affect the growth curves) and was found non-toxic in cell assay and brine shrimp lethality assay. Conclusion According to the result obtained, the designed formulation was found effective and non-toxic, so it can be used to treat shigellosis and Shigella-related infections.
Structure based virtual screening for identification of potential quorum sensing inhibitors against LasR master regulator in Pseudomonas aeruginosa
Microbial Pathogenesis, Jun 1, 2017
Inter and intracellular communication in bacteria, which is known as quorum sensing (QS), is medi... more Inter and intracellular communication in bacteria, which is known as quorum sensing (QS), is mediated by small diffusible signaling molecules known as autoinducers. QS regulates various virulence factors responsible for pathogenesis. Increasing resistance of microorganisms against traditional antibiotics has turned the focus towards the QS as it exerts less selective pressure preventing development of resistance among microorganisms. LasR, a transcription factor that controls QS in Pseudomonas aeruginosa, is an attractive therapeutic target for inhibitors. This study aimed to screen natural compounds as potential inhibitors of LasR. About 2603 compounds from ZINC database were virtually screened against the structure of LasR. Then after qualifying compounds were filtered on the parameters of Lipinski's rule and ADME. Six novel potential QS inhibiting compounds were selected on the basis of binding energy. Structures of LasR-ligand complexes were analysed to have insight of binding between inhibitors and target. It is pertinent to mention here that all the molecules are structurally different from 3-oxo-C12HSL,a native autoinducer of LasR, that play key role in formation of LasR dimer which is an active form of the protein to facilitate QS.
Data Regarding Anti-Quorum Sensing and Antimicrobial Activity of Melaleuca Alternifolia and Salvia Sclarea Essential Oil Against Pseudomonas Aeruginosa
SSRN Electronic Journal
Mathematics and Statistics
Transparency order is considered to be a cryptographically significant property that characterize... more Transparency order is considered to be a cryptographically significant property that characterizes the resistance of S-boxes in opposition to differential power analysis attacks. The S-box having low transparency order is more resistant to these attacks. Until now, little attempts have been noticed to examine theoretically the transparency order and its relationship with other cryptographic properties. All constructions associated with transparency order are relying on search algorithms. In this paper, we discuss the new interpretation of bent functions in terms of their transparency order. Using the concept of vector concatenation and correlation characteristics, we find the transparency order of Boolean functions. The notion of complementary transparency order is given. For a pair of Boolean functions, we interpret complementary transparency order by their Walsh-Hadamard transform. We establish a relationship of transparency order with cross-correlation for a pair of Boolean functions. We find a relationship of transparency order with (n − 2)−variable decomposition bent functions. We generalize the bounds on sum-of-squares of autocorrelation in terms of transparency order of Boolean functions using Walsh-Hadamard spectra. Further the transparency order of a function fulfilling the propagation criterion about a linear subspace is evaluated.
Journal of Applied Biomedicine, 2022
Bacillary dysentery (shigellosis) continues to cause havoc worldwide, with a high infectivity rat... more Bacillary dysentery (shigellosis) continues to cause havoc worldwide, with a high infectivity rate. It causes bloody diarrhea, and around 99% of bacillary dysentery cases occur in developing countries. The objective of this study is to develop a polyherbal formulation with the scientific rationale in treating infectious bacillary dysentery disease. The anti-bacterial activity, the minimum inhibitory concentration of the formulation against bacillary dysentery, causing microbes like Shigella flexneri (MTCC 1457), Escherichia coli (MTCC 1687), and Salmonella enterica (MTCC 98), was analysed by well-diffusion method and broth dilution method, respectively. The biofilm inhibition activity was determined on 96 well polystyrene plates and anti-quorum sensing activity by Chromobacterium violaceum CV026. The cytotoxicity was examined by acute oral toxicity. Excreta and organ bacterial load were analyzed by serial dilution method. The formulation efficacy was determined by analyzing the blood sample of rats. The antimicrobial efficacy of the developed formulation was calculated by measuring the zone of inhibition which was found to be 24 mm, 25 mm, and 25 mm, and the MIC values of 1.5 mg/ml, 1.5 mg/ml, and 2.0 mg/ml against S. flexneri, S. enterica, E. coli, respectively. The results show that the polyherbal formulation significantly reduced biofilm formation and has anti-quorum sensing activity. The formulation also effectively decreases the bacterial load and increases the K + , Na + , and Ca ++ ions in animals treated with the formulation. The developed formulation was found to be non-toxic and effective against bacillary dysentery; thus, it can be used for treating bacillary dysentery and related complications.
Journal of Experimental Biology and Agricultural Sciences, 2021
Staphylococcus epidermidis and Pseudomonas aeruginosa, are clinically relevant pathogens that oft... more Staphylococcus epidermidis and Pseudomonas aeruginosa, are clinically relevant pathogens that often produce biofilms. To investigate the co-survivability of S. epidermidis and P. aeruginosa in mixed cultures biofilm and planktonic form, it is important to understand more about the interspecies interaction of both species. The interspecies interaction was analyzed using streak and drop agar plate assay, cell viability assay (CFU), spectrophotometry-based method, and microscopic analysis. The findings suggest that both cells and supernatant of P. aeruginosa inhibit the planktonic growth of S. epidermidis. The cell viability result shows that PAO1 biofilm cells were decreased by 88%, and SE biofilm cells were increased by 75% concerning their control. Opposite to the P. aeruginosa, the S. epidermidis biofilm and EPS matrix were found to increase in mixed culture biofilm, which was further confirmed by microscopic analysis. In contrast, differential agar media result shows that the redu...
RESEARCH ARTICLE Effect of Cinnamon Oil on Quorum Sensing- Controlled Virulence Factors and Biofilm Formation in Pseudomonas aeruginosa
Quorum sensing (QS) is a system of stimuli and responses in bacterial cells governed by their pop... more Quorum sensing (QS) is a system of stimuli and responses in bacterial cells governed by their population density, through which they regulate genes that control virulence factors and biofilm formation. Despite considerable research on QS and the discovery of new anti-biotics, QS-controlled biofilm formation by microorganisms in clinical settings has remained a problem because of nascent drug resistance, which requires screening of diverse com-pounds for anti-QS activities. Cinnamon is a dietary phytochemical that is traditionally used to remedy digestive problems and assorted contagions, which suggests that cinnamon might contain chemicals that can hinder the QS process. To test this hypothesis, the anti-QS activity of cinnamon oil against P. aeruginosa was tested, measured by the inhibition of biofilm formation and other QS-associated phenomena, including virulence factors such as pyocyanin, rhamnolipid, protease, alginate production, and swarming activity. To this end, multiple mi...
Journal of Applied Biology & Biotechnology, 2021
Staphylococcus epidermidis and Pseudomonas aeruginosa are the two most commonly detected bacteria... more Staphylococcus epidermidis and Pseudomonas aeruginosa are the two most commonly detected bacteria. Pseudomonas aeruginosa exerts significant impacts on pathogenesis through quorum-sensing regulation, while S. epidermidis role has always remained a matter of interest due to its frequent presence in most of the hospitalized samples and even inside the body during operation procedures. In this study, the effect of S. epidermidis cells on P. aeruginosa pathogenesis was analyzed. The effect of S. epidermidis cells on P. aeruginosa growth was analyzed by using microtiter plate assay, Colony forming unit (CFU), and microscopy. The effect of virulence factors including protease, rhamnolipid, and swarming motility was also investigated. Escherichia coli pJN105LpSC11, a bioreporter strain, was used to analyze the effect of S. epidermidis on P. aeruginosa quorum-sensing. The growth of P. aeruginosa did not affect S. epidermidis cells of 5.5x10 9 , 6x10 9 , 7x10 9 , 8.5x10 9 , and 1.5x10 10 CFU/ml, and microscopy results are consistent with the findings. P. aeruginosaassociated virulence factors show that with increasing S. epidermidis counts, P. aeruginosa-associated virulence factors were reduced. No effect of S. epidermidis on P. aeruginosa quorum-sensing was observed. The outcomes suggest that S. epidermidis can be used as an alternative to reduce Pseudomonas aeruginosaassociated virulence factors and its pathogenesis.
Mathematics and Statistics
From the last few years, generalized bent functions gain a lot of attention in research as they h... more From the last few years, generalized bent functions gain a lot of attention in research as they have many applications in various fields such as combinatorial design, sequence design theory, cryptography, CDMA communication, etc. A deep and broad study of generalized bent functions with their properties is done in literature. Kumar et al.[11] first gave the concept of generalized bent function. Many researchers studied the properties and characterizations of generalized bent functions. In [2] authors introduced the concept of generalized (q-ary) negabent functions and studied some properties of generalized (q-ary) negabent functions. In this paper, we study the generalized (q-ary) bent functions f : Z n q → Z q , where Z q is the ring of integers with mod q, Z n q is the vector space of dimension n over Z q and q ≥ 2 is any positive integer. We discuss several properties of generalized (q-ary) bent functions with respect to their nega-Hadamard transform. We also study the relation between generalized nega-Hadamard transforms and generalized nega-autocorrelations. Furthermore, we prove the necessary and sufficient conditions for the bentness and negabentness of generalized (q-ary) bent function generated by the secondary construction for Z n q , where q = 2 s .
Senna alexandriana mill as a potential inhibitor for quorum sensing-controlled virulence factors and biofilm formation in Pseudomonas aeruginosa PAO1
Pharmacognosy Magazine
Background: Senna alexandriana Mill is a medicinally important plant used in laxatives and also p... more Background: Senna alexandriana Mill is a medicinally important plant used in laxatives and also possesses potent antimicrobial compounds, but its effect on the Pseudomonas aeruginosa was never analyzed. Aim: This study was carried out with the objective to attenuate quorum sensing (QS)-controlled virulence factors in P. aeruginosa and its biofilm. Methods: In the present study, acetone and hexane extracts of S. alexandriana mill were tested at sub-minimum inhibitory concentration concentrations for their anti-QS activity using bioreporter strain the Chromobacterium violaceum CV026. The effect of S. alexandriana Mill on different virulence phenotypes such as pyocyanin and protease production was investigated. Finally, the effect of the extract on P. aeruginosa, biofilm formation was also determined using the spectrophotometry-based method and microscopic analysis. Results: The extracts were tested for their activities against QS-controlled virulence factors of P. aeruginosa at 600 μg/ml and 800 μg/ml acetone and hexane extract concentration, respectively. Results showed that both the extracts have an inhibitory effect on different virulence factors of P. aeruginosa, including pyocyanin, protease production, and biofilm formation, without affecting the growth. The P. aeruginosa biofilm was found to be decreased by 75% and 62% in acetone and hexane extract presence. Conclusion: The results revealed that both acetone and hexane extracts showed anti-biofilm and anti-QS activity. It depicted the potential of S. alexandriana Mill against P. aeruginosa infection, its biofilm, and QS.
Pseudomonas aeruginosa quorum-sensing molecule N-(3-oxo-dodecanoyl)-l-homoserine lactone triggers mitochondrial dysfunction and apoptosis in neutrophils through calcium signaling
Medical Microbiology and Immmunology, Aug 3, 2019
Pseudomonas aeruginosa is an opportunistic pathogen that utilizes the quorum-sensing (QS) process... more Pseudomonas aeruginosa is an opportunistic pathogen that utilizes the quorum-sensing (QS) process to regulate the production of different virulence factors and biofilm. N-3-oxo-dodecanoyl-L-homoserine lactone (C12) is a key QS molecule of P. aeruginosa which interacts with the mammalian immune cells and modulates their function. Here, we investigated the molecular mechanism of C12-induced apoptosis in neutrophils. Our data show that C12 causes apoptosis in neutrophils through an elevation in cytosolic and mitochondrial Ca2+ levels. Besides, C12 induces phosphatidylserine (PS) exposure, mitochondrial membrane potential (MMP) depolarization, mitochondrial permeability transition pore (MPTP) formation and mitochondrial reactive oxygen species (mROS) generation. C12-induced rise in intracellular Ca2+ level is majorly contributed by endoplasmic reticulum store through the activation of inositol 1, 4, 5-triphosphate receptor. Intracellular calcium chelation inhibited C12-induced mitochond...
IACR Cryptology ePrint Archive, 2008
It is proved that no non-quadratic Kasami bent is affine equivalent to Maiorana-MacFarland type b... more It is proved that no non-quadratic Kasami bent is affine equivalent to Maiorana-MacFarland type bent functions.
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Papers by Deepmala Sharma