RESUMO.-[Causas de morte e eutanásia em felinos domésticos durante os primeiros anos da pandemia ... more RESUMO.-[Causas de morte e eutanásia em felinos domésticos durante os primeiros anos da pandemia de SARS-CoV-2 (2020 e 2021): um estudo multi-institucional de necropsias entre Rio de Janeiro e Mato Grosso.] Estudos retrospectivos que abordam doenças em felinos domésticos são escassos. Apresentamos aqui a causa da morte ou razões para eutanásia de gatos domésticos entre janeiro de 2020 e dezembro de 2021, durante o primeiro e segundo ano da pandemia de SARS-CoV-2. Os dados foram obtidos em necropsias realizadas pela Universidade Federal Rural do Rio de Janeiro e Universidade Federal de Mato Grosso. Foram realizadas 96 necropsias de felinos. Em 87 casos (90,6%) foi estabelecido a causa da morte e em nove casos (9,4%) os diagnósticos foram inconclusivos. Estabelecemos os grupos diagnósticos: infecciosos e parasitários (37,5%), neoplasias (14,5%), malformações (7,3%), doenças do trato urinário inferior (7,3%), degenerativas (6,2%), traumas (6,2%), outras causas (8,4%) e iatrogênicas (3,1%). As doenças mais frequentes em gatos do Mato Grosso e Rio de Janeiro foram as infecciosas. As lesões inflamatórias mais frequentes foram as pneumonias bacterianas e virais. Os principais agentes detectados foram Alphaherpesvirus (FeHV), Mycoplasma sp. e Pseudomonas sp. TERMOS DE INDEXAÇÃO: Doenças infecciosas, doenças de felinos, felinos, pneumonia, imuno-histoquímica, RT-qPCR.
A 10‐year‐old black howler monkey presented with a 36‐day subacute clinicopathological picture of... more A 10‐year‐old black howler monkey presented with a 36‐day subacute clinicopathological picture of fever, prostration, inappetence, intestinal hypomotility, and emaciation. Therapy was trimethoprim‐sulfamethoxazole with streptomycin. The liver, lungs, lymph nodes, and spleen presented lesions. Toxoplasma gondii isolation and PCR determined the diagnosis, and indirect fluorescent antibody tests confirmed an increase in antibody titers.
The epidemiology, clinical signs, and pathology of 42 outbreaks of enzootic calcinosis (EC) in ca... more The epidemiology, clinical signs, and pathology of 42 outbreaks of enzootic calcinosis (EC) in cattle, sheep and goats in Mato Grosso (19), Distrito Federal (19), Goiás, (6), Minas Gerais (2) and Tocantins (1), Brazil, [date not given] are presented. Additionally, Ageratum conyzoides, Chamaecrista desvauxii [Cassia desvauxii], Cissus erosa, Elephantopus mollis, Eragrostis sp., Eupatorium odoratum [Chromolaena odorata], Hymenaea courbaril, Jatropha sp., Ludwigia octovalvis, Mimosa hirsutissima, Murdania nudiflora, Rhynchanthera novemnervia, Scoparia dulcis, Sida santaremensis, Tibouchina sp., Vernonia brasiliana, Waltheria sp., Arachis sp., Cnidoscolus cnicadendron, Eleusine tristachya, Ludwigia leptocarpa, Ludwigia sp., Mimosa pellita, Sida cerradoensis, Sida ciliaris, Sida spinosa and Vernonia ferruginea as the possible causes for the outbreaks were experimentally administered to rabbits and sheep. It was shown that none of the 33 rabbits and 14 sheep that ingested the selected weeds to detect calcinogenic effect of the plants developed clinical changes, and mineralization of soft tissues was not observed during necropsies or histologic examination. Serum Ca and P concentrations were within normal values.
International journal for parasitology. Parasites and wildlife, Dec 1, 2022
Toxoplasma gondii was isolated in mice from different tissues of a captive black-and-gold howler ... more Toxoplasma gondii was isolated in mice from different tissues of a captive black-and-gold howler monkey (Alouatta caraya) kept in a colony at the Primatology Center of Rio de Janeiro State, Brazil, and it was genotypically characterized based on using PCR-RFLP and Microsatellite Analysis (MS), later on. T. gondii was successfully isolated from inocula deriving from heart, liver and tissue pool (heart, liver, lungs, axillary lymph nodes and cerebellum) samples. The isolate was named TgBgHmBrRJ1. The high virulence of the aforementioned strain was observed in infected mice. Non-archetypal genotype (ToxoDB PCR-RFLP #206) was obtained through PCR-RFLP. This genotype had been previously described in 12 isolates from different hosts, also in Southeastern Brazil, a fact that indicates likely high circulation of this genotype in this region. The isolate was also classified as non-archetypal, based on MS genotyping, as well as presented genotypic identity close to that of strains isolated from free-range non-symptomatic chickens (TgCkBr244,245,278,279) in Espírito Santo State. It is worth emphasizing that despite the large number of reports about clinical toxoplasmosis in neotropical primates in Brazil, this is just the second isolate of this parasite ever reported in this group of animals.
Primary female reproductive neoplasms in Platyrrhines species are few reported. We present the gr... more Primary female reproductive neoplasms in Platyrrhines species are few reported. We present the gross, histological, and immunohistochemical findings of metastatic endometrioid carcinoma in the uterus, urinary bladder, jejunum, and rectum of a Leontopithecus sp. The neoplastic endometrial cells expressed strong cytoplasmic immunolabeling of cytokeratin 7.
Colic outbreaks in horses have been associated with the grazing of several Megathyrsus maximus (S... more Colic outbreaks in horses have been associated with the grazing of several Megathyrsus maximus (Sin. Panicum maximum) cultivars in the North and Central-West regions of Brazil. In this paper, we report a horse colic outbreak in the Southeast region of Brazil caused by ingestion of the "Colonião" cultivar of M. maximus, which has not previously been considered as toxic. The five affected horses belonged to the Veterinary Platoon based at the Central Ammunition Deposit of the Brazilian Army in the city of Paracambi, Rio de Janeiro state, Brazil. The horses had access to treated water and commercial concentrate, and were located in a field of M. maximus at the time of the outbreak. All horses exhibited clinical signs of colic and bloat, and three of them died. The extend of the clinical course ranged from four to five days in the three animals that died; in the two animals that recovered from the colic episodes, the extend of the clinical courses were 10 and 15 days. Necropsy findings revealed intestinal and gastric bloating and hemorrhages involving the intestinal wall. Light microscopy showed moderate diffuse lymphoplasmacytic and eosinophilic enteritis with multifocal erosions, in addition to submucosal edema associated with multifocal vasculitis. The pathogenesis of colic caused by M. maximus ingestion in horses has not yet been elucidated. Some authors have suggested that higher starch concentrations in M. maximus during the rainy season may be responsible for the toxicity of this plant. However, the findings of this study do not support this hypothesis. As a prophylactic measure, it is suggested that horses do not graze exclusively M. maximus at the beginning of rainy periods, in which regrowth of this grass occurs. In Brazil, outbreaks of horse colic associated with ingestion of varieties of Megathyrsus can also occur outside the North and Midwest regions, under specific climate conditions.
Introducao: A ruminite enϐisematosa foi descrita por Ohfuji (2015) em 20 bovinos abatidos em frig... more Introducao: A ruminite enϐisematosa foi descrita por Ohfuji (2015) em 20 bovinos abatidos em frigoriϐicos. A lesao caracterizou-se como uma linfangite na submucosa com inϐiltrado granulomatoso, eosinoϐilos e sem presenca de microrganismos. Lesoes semelhantes no intestino de suinos sao caracterizadas pela presenca de multiplos vasos linfaticos dilatados por conteudo gasoso nas camadas subserosa e submucosa da parede intestinal, tecido mesenterico e linfonodos (Salles et al. 2008). O objetivo deste trabalho e relatar o diagnostico histopatologico de um caso de ruminite enϐisematosa em um bovino Nelore confinado.
Arquivo Brasileiro De Medicina Veterinaria E Zootecnia, Dec 1, 2022
Clinical parameters and calf liveweight of Nellore breeders supplemented or not with phosphorus, ... more Clinical parameters and calf liveweight of Nellore breeders supplemented or not with phosphorus, during pregnancy and lactationa case study [Parâmetros clínicos e peso de bezerros Nelore cujas matrizes foram suplementadas ou não com fósforo, durante a gestação e a lactação-estudo de caso]
The Amazon biome has a great diversity of bat species. In the state of Acre, Brazil, there is an ... more The Amazon biome has a great diversity of bat species. In the state of Acre, Brazil, there is an estimated occurrence of 64 bat species with the species of the genus Artibeus as one of the most abundant. Despite their abundance and widespread distribution within the biome, the helminth fauna from Amazonian bats is still poorly known. In this way, the objective of this study is to describe a new species of cestode from the genus Vampirolepis found in A. lituratus, collected at the Parque Estadual do Chandless, a natural preserved area, located in the Purus River Basin, Southwest Amazon, the state of Acre. The new species of Vampirolepis is distinguished from the others by the number and size of hooks, testes disposition, size of the cirrus sac, ovaries and internal and external seminal vesicles. Additionally, molecular study showed that this forms a paraphyletic clade with Vampirolepis elongatus.
Este estudo avaliou a composição e os requisitos mínimos de identidade e qualidade da carne bovin... more Este estudo avaliou a composição e os requisitos mínimos de identidade e qualidade da carne bovina previamente moída exposta à venda em estabelecimentos varejistas no Estado do Rio de Janeiro. As amostras foram coletadas nos estabelecimentos no período entre março e abril de 2019. Os produtos foram analisados quanto a composição, a apresentação, a temperatura, o pH, teor de lipídios, morfologia e histoquímica, e a identificação da espécie de animal que originou a matéria prima do produto. De 11 amostras coletadas, 72,7% foram moídas e armazenadas no próprio estabelecimento varejista e 27,3% foram previamente moídas e embaladas nos estabelecimentos industriais. Em 18,2% das amostras foi identificado teor de gordura 34% acima da quantidade máxima aceitável, apresentando alterações nas características sensoriais, como aspecto pegajoso, odor não característico e pH > 6,4. Foi observada perda de estriação nas fibras musculares esqueléticas e dos detalhes celulares em 54,5% das amostra...
RESUMO.-[Causas de morte e eutanásia em felinos domésticos durante os primeiros anos da pandemia ... more RESUMO.-[Causas de morte e eutanásia em felinos domésticos durante os primeiros anos da pandemia de SARS-CoV-2 (2020 e 2021): um estudo multi-institucional de necropsias entre Rio de Janeiro e Mato Grosso.] Estudos retrospectivos que abordam doenças em felinos domésticos são escassos. Apresentamos aqui a causa da morte ou razões para eutanásia de gatos domésticos entre janeiro de 2020 e dezembro de 2021, durante o primeiro e segundo ano da pandemia de SARS-CoV-2. Os dados foram obtidos em necropsias realizadas pela Universidade Federal Rural do Rio de Janeiro e Universidade Federal de Mato Grosso. Foram realizadas 96 necropsias de felinos. Em 87 casos (90,6%) foi estabelecido a causa da morte e em nove casos (9,4%) os diagnósticos foram inconclusivos. Estabelecemos os grupos diagnósticos: infecciosos e parasitários (37,5%), neoplasias (14,5%), malformações (7,3%), doenças do trato urinário inferior (7,3%), degenerativas (6,2%), traumas (6,2%), outras causas (8,4%) e iatrogênicas (3,1%). As doenças mais frequentes em gatos do Mato Grosso e Rio de Janeiro foram as infecciosas. As lesões inflamatórias mais frequentes foram as pneumonias bacterianas e virais. Os principais agentes detectados foram Alphaherpesvirus (FeHV), Mycoplasma sp. e Pseudomonas sp. TERMOS DE INDEXAÇÃO: Doenças infecciosas, doenças de felinos, felinos, pneumonia, imuno-histoquímica, RT-qPCR.
A 10‐year‐old black howler monkey presented with a 36‐day subacute clinicopathological picture of... more A 10‐year‐old black howler monkey presented with a 36‐day subacute clinicopathological picture of fever, prostration, inappetence, intestinal hypomotility, and emaciation. Therapy was trimethoprim‐sulfamethoxazole with streptomycin. The liver, lungs, lymph nodes, and spleen presented lesions. Toxoplasma gondii isolation and PCR determined the diagnosis, and indirect fluorescent antibody tests confirmed an increase in antibody titers.
The epidemiology, clinical signs, and pathology of 42 outbreaks of enzootic calcinosis (EC) in ca... more The epidemiology, clinical signs, and pathology of 42 outbreaks of enzootic calcinosis (EC) in cattle, sheep and goats in Mato Grosso (19), Distrito Federal (19), Goiás, (6), Minas Gerais (2) and Tocantins (1), Brazil, [date not given] are presented. Additionally, Ageratum conyzoides, Chamaecrista desvauxii [Cassia desvauxii], Cissus erosa, Elephantopus mollis, Eragrostis sp., Eupatorium odoratum [Chromolaena odorata], Hymenaea courbaril, Jatropha sp., Ludwigia octovalvis, Mimosa hirsutissima, Murdania nudiflora, Rhynchanthera novemnervia, Scoparia dulcis, Sida santaremensis, Tibouchina sp., Vernonia brasiliana, Waltheria sp., Arachis sp., Cnidoscolus cnicadendron, Eleusine tristachya, Ludwigia leptocarpa, Ludwigia sp., Mimosa pellita, Sida cerradoensis, Sida ciliaris, Sida spinosa and Vernonia ferruginea as the possible causes for the outbreaks were experimentally administered to rabbits and sheep. It was shown that none of the 33 rabbits and 14 sheep that ingested the selected weeds to detect calcinogenic effect of the plants developed clinical changes, and mineralization of soft tissues was not observed during necropsies or histologic examination. Serum Ca and P concentrations were within normal values.
International journal for parasitology. Parasites and wildlife, Dec 1, 2022
Toxoplasma gondii was isolated in mice from different tissues of a captive black-and-gold howler ... more Toxoplasma gondii was isolated in mice from different tissues of a captive black-and-gold howler monkey (Alouatta caraya) kept in a colony at the Primatology Center of Rio de Janeiro State, Brazil, and it was genotypically characterized based on using PCR-RFLP and Microsatellite Analysis (MS), later on. T. gondii was successfully isolated from inocula deriving from heart, liver and tissue pool (heart, liver, lungs, axillary lymph nodes and cerebellum) samples. The isolate was named TgBgHmBrRJ1. The high virulence of the aforementioned strain was observed in infected mice. Non-archetypal genotype (ToxoDB PCR-RFLP #206) was obtained through PCR-RFLP. This genotype had been previously described in 12 isolates from different hosts, also in Southeastern Brazil, a fact that indicates likely high circulation of this genotype in this region. The isolate was also classified as non-archetypal, based on MS genotyping, as well as presented genotypic identity close to that of strains isolated from free-range non-symptomatic chickens (TgCkBr244,245,278,279) in Espírito Santo State. It is worth emphasizing that despite the large number of reports about clinical toxoplasmosis in neotropical primates in Brazil, this is just the second isolate of this parasite ever reported in this group of animals.
Primary female reproductive neoplasms in Platyrrhines species are few reported. We present the gr... more Primary female reproductive neoplasms in Platyrrhines species are few reported. We present the gross, histological, and immunohistochemical findings of metastatic endometrioid carcinoma in the uterus, urinary bladder, jejunum, and rectum of a Leontopithecus sp. The neoplastic endometrial cells expressed strong cytoplasmic immunolabeling of cytokeratin 7.
Colic outbreaks in horses have been associated with the grazing of several Megathyrsus maximus (S... more Colic outbreaks in horses have been associated with the grazing of several Megathyrsus maximus (Sin. Panicum maximum) cultivars in the North and Central-West regions of Brazil. In this paper, we report a horse colic outbreak in the Southeast region of Brazil caused by ingestion of the "Colonião" cultivar of M. maximus, which has not previously been considered as toxic. The five affected horses belonged to the Veterinary Platoon based at the Central Ammunition Deposit of the Brazilian Army in the city of Paracambi, Rio de Janeiro state, Brazil. The horses had access to treated water and commercial concentrate, and were located in a field of M. maximus at the time of the outbreak. All horses exhibited clinical signs of colic and bloat, and three of them died. The extend of the clinical course ranged from four to five days in the three animals that died; in the two animals that recovered from the colic episodes, the extend of the clinical courses were 10 and 15 days. Necropsy findings revealed intestinal and gastric bloating and hemorrhages involving the intestinal wall. Light microscopy showed moderate diffuse lymphoplasmacytic and eosinophilic enteritis with multifocal erosions, in addition to submucosal edema associated with multifocal vasculitis. The pathogenesis of colic caused by M. maximus ingestion in horses has not yet been elucidated. Some authors have suggested that higher starch concentrations in M. maximus during the rainy season may be responsible for the toxicity of this plant. However, the findings of this study do not support this hypothesis. As a prophylactic measure, it is suggested that horses do not graze exclusively M. maximus at the beginning of rainy periods, in which regrowth of this grass occurs. In Brazil, outbreaks of horse colic associated with ingestion of varieties of Megathyrsus can also occur outside the North and Midwest regions, under specific climate conditions.
Introducao: A ruminite enϐisematosa foi descrita por Ohfuji (2015) em 20 bovinos abatidos em frig... more Introducao: A ruminite enϐisematosa foi descrita por Ohfuji (2015) em 20 bovinos abatidos em frigoriϐicos. A lesao caracterizou-se como uma linfangite na submucosa com inϐiltrado granulomatoso, eosinoϐilos e sem presenca de microrganismos. Lesoes semelhantes no intestino de suinos sao caracterizadas pela presenca de multiplos vasos linfaticos dilatados por conteudo gasoso nas camadas subserosa e submucosa da parede intestinal, tecido mesenterico e linfonodos (Salles et al. 2008). O objetivo deste trabalho e relatar o diagnostico histopatologico de um caso de ruminite enϐisematosa em um bovino Nelore confinado.
Arquivo Brasileiro De Medicina Veterinaria E Zootecnia, Dec 1, 2022
Clinical parameters and calf liveweight of Nellore breeders supplemented or not with phosphorus, ... more Clinical parameters and calf liveweight of Nellore breeders supplemented or not with phosphorus, during pregnancy and lactationa case study [Parâmetros clínicos e peso de bezerros Nelore cujas matrizes foram suplementadas ou não com fósforo, durante a gestação e a lactação-estudo de caso]
The Amazon biome has a great diversity of bat species. In the state of Acre, Brazil, there is an ... more The Amazon biome has a great diversity of bat species. In the state of Acre, Brazil, there is an estimated occurrence of 64 bat species with the species of the genus Artibeus as one of the most abundant. Despite their abundance and widespread distribution within the biome, the helminth fauna from Amazonian bats is still poorly known. In this way, the objective of this study is to describe a new species of cestode from the genus Vampirolepis found in A. lituratus, collected at the Parque Estadual do Chandless, a natural preserved area, located in the Purus River Basin, Southwest Amazon, the state of Acre. The new species of Vampirolepis is distinguished from the others by the number and size of hooks, testes disposition, size of the cirrus sac, ovaries and internal and external seminal vesicles. Additionally, molecular study showed that this forms a paraphyletic clade with Vampirolepis elongatus.
Este estudo avaliou a composição e os requisitos mínimos de identidade e qualidade da carne bovin... more Este estudo avaliou a composição e os requisitos mínimos de identidade e qualidade da carne bovina previamente moída exposta à venda em estabelecimentos varejistas no Estado do Rio de Janeiro. As amostras foram coletadas nos estabelecimentos no período entre março e abril de 2019. Os produtos foram analisados quanto a composição, a apresentação, a temperatura, o pH, teor de lipídios, morfologia e histoquímica, e a identificação da espécie de animal que originou a matéria prima do produto. De 11 amostras coletadas, 72,7% foram moídas e armazenadas no próprio estabelecimento varejista e 27,3% foram previamente moídas e embaladas nos estabelecimentos industriais. Em 18,2% das amostras foi identificado teor de gordura 34% acima da quantidade máxima aceitável, apresentando alterações nas características sensoriais, como aspecto pegajoso, odor não característico e pH > 6,4. Foi observada perda de estriação nas fibras musculares esqueléticas e dos detalhes celulares em 54,5% das amostra...
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