FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 2015
β1 integrins (β1) transduce mechanical signals in many cells, including cardiac myocytes (CM). Gi... more β1 integrins (β1) transduce mechanical signals in many cells, including cardiac myocytes (CM). Given their close localization, as well as their role in mechanotransduction and signaling, we hypothesized that caveolin (Cav) proteins might regulate integrins in the CM. β1 localization, complex formation, activation state, and signaling were analyzed using wild-type, Cav3 knockout, and Cav3 CM-specific transgenic heart and myocyte samples. Studies were performed under basal and mechanically loaded conditions. We found that: 1) β1 and Cav3 colocalize in CM and coimmunoprecipitate from CM protein lysates; 2) β1 is detected in a subset of caveolae; 3) loss of Cav3 caused reduction of β1D integrin isoform and active β1 integrin from the buoyant domains in the heart; 4) increased expression of myocyte Cav3 correlates with increased active β1 integrin in adult CM; 5) in vivo pressure overload of the wild-type heart results in increased activated integrin in buoyant membrane domains along wit...
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 2015
β1 integrins (β1) transduce mechanical signals in many cells, including cardiac myocytes (CM). Gi... more β1 integrins (β1) transduce mechanical signals in many cells, including cardiac myocytes (CM). Given their close localization, as well as their role in mechanotransduction and signaling, we hypothesized that caveolin (Cav) proteins might regulate integrins in the CM. β1 localization, complex formation, activation state, and signaling were analyzed using wild-type, Cav3 knockout, and Cav3 CM-specific transgenic heart and myocyte samples. Studies were performed under basal and mechanically loaded conditions. We found that: 1) β1 and Cav3 colocalize in CM and coimmunoprecipitate from CM protein lysates; 2) β1 is detected in a subset of caveolae; 3) loss of Cav3 caused reduction of β1D integrin isoform and active β1 integrin from the buoyant domains in the heart; 4) increased expression of myocyte Cav3 correlates with increased active β1 integrin in adult CM; 5) in vivo pressure overload of the wild-type heart results in increased activated integrin in buoyant membrane domains along wit...
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 2015
β1 integrins (β1) transduce mechanical signals in many cells, including cardiac myocytes (CM). Gi... more β1 integrins (β1) transduce mechanical signals in many cells, including cardiac myocytes (CM). Given their close localization, as well as their role in mechanotransduction and signaling, we hypothesized that caveolin (Cav) proteins might regulate integrins in the CM. β1 localization, complex formation, activation state, and signaling were analyzed using wild-type, Cav3 knockout, and Cav3 CM-specific transgenic heart and myocyte samples. Studies were performed under basal and mechanically loaded conditions. We found that: 1) β1 and Cav3 colocalize in CM and coimmunoprecipitate from CM protein lysates; 2) β1 is detected in a subset of caveolae; 3) loss of Cav3 caused reduction of β1D integrin isoform and active β1 integrin from the buoyant domains in the heart; 4) increased expression of myocyte Cav3 correlates with increased active β1 integrin in adult CM; 5) in vivo pressure overload of the wild-type heart results in increased activated integrin in buoyant membrane domains along wit...
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 2015
β1 integrins (β1) transduce mechanical signals in many cells, including cardiac myocytes (CM). Gi... more β1 integrins (β1) transduce mechanical signals in many cells, including cardiac myocytes (CM). Given their close localization, as well as their role in mechanotransduction and signaling, we hypothesized that caveolin (Cav) proteins might regulate integrins in the CM. β1 localization, complex formation, activation state, and signaling were analyzed using wild-type, Cav3 knockout, and Cav3 CM-specific transgenic heart and myocyte samples. Studies were performed under basal and mechanically loaded conditions. We found that: 1) β1 and Cav3 colocalize in CM and coimmunoprecipitate from CM protein lysates; 2) β1 is detected in a subset of caveolae; 3) loss of Cav3 caused reduction of β1D integrin isoform and active β1 integrin from the buoyant domains in the heart; 4) increased expression of myocyte Cav3 correlates with increased active β1 integrin in adult CM; 5) in vivo pressure overload of the wild-type heart results in increased activated integrin in buoyant membrane domains along wit...
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Papers by Daniel Deussen