Papers by Danica Ljubanović
Croatian Medical Journal, 2017
To determine the role of immunoglobulin M (IgM) deposits in clinical manifestations, disease outc... more To determine the role of immunoglobulin M (IgM) deposits in clinical manifestations, disease outcome, and treatment response of idiopathic and secondary focal segmental glomerulosclerosis (FSGS). Methods Kidney biopsy specimens of 171 patients diagnosed with FSGS (primary and secondary) and 50 control patients were retrospectively included in the study. For each patient, clinical and outcome data were obtained and compared to morphological parameters, including immunofluorescence analysis of mesangial IgM and complement 3 (C3) deposits analyzed on kidney biopsy samples. There were significant positive correlations between IgM and C3 deposition in secondary FSGS (P < 0.001) and between IgM and mesangial deposits detected by electron microscopy in secondary FSGS (P = 0.015), which indicated that higher IgM deposition correlated with higher C3 deposition and mesangial deposits only in secondary FSGS. Patients with secondary FSGS and the deposition of IgM showed inferior renal outcomes at earlier time points in comparison with patients with negative IgM expression (P = 0.022). We detected a positive correlation between IgM and C3 in secondary FSGS. The association between IgM deposition and worse renal outcome in secondary FSGS indicates that IgM may play a role in the progression of this disease.
Kidney international, Jan 28, 2015
The Oxford Classification of IgA nephropathy (IgAN) includes four histologic components: mesangia... more The Oxford Classification of IgA nephropathy (IgAN) includes four histologic components: mesangial (M) and endocapillary (E) hypercellularity, segmental sclerosis (S) and interstitial fibrosis/tubular atrophy (T). These combine to form the MEST score and are independently associated with renal outcome. Current prediction and risk stratification in IgAN requires clinical data over 2 years of follow-up. Using modern prediction tools, we examined whether combining MEST with cross-sectional clinical data at biopsy provides earlier risk prediction in IgAN than current best methods that use 2 years of follow-up data. We used a cohort of 901 adults with IgAN from the Oxford derivation and North American validation studies and the VALIGA study followed for a median of 5.6 years to analyze the primary outcome (50% decrease in eGFR or ESRD) using Cox regression models. Covariates of clinical data at biopsy (eGFR, proteinuria, MAP) with or without MEST, and then 2-year clinical data alone (2-y...
Izolirana sarkoidoza bubrega: prikaz bolesnice
5. Hrvatski kongres …, 2008
Liječnički vjesnik, 2022
Autori su u radu prikazali život i rad Marcela Kornfelda (1886. – 1937.), patologa i sveučilišnog... more Autori su u radu prikazali život i rad Marcela Kornfelda (1886. – 1937.), patologa i sveučilišnog nastavnika. U radu je kronološki prikazan Kornfeldov život, od njegovih početaka u Donjoj Tuzli do njegove smrti u Zagrebu. Istaknuti su najvažniji detalji iz njegova života i bogate karijere koju je prekinula smrt u 51. godini života. Uz literaturu, pri nastanku rada veliku važnost su imali arhivski izvori pohranjeni na Medicinskom fakultetu Sveučilišta u Zagrebu i u Državnom arhivu u Zagrebu.
European Urology Supplements, 2005
investigated the raised transmembrane oxalate transport in human red blood cells in idiopathic ca... more investigated the raised transmembrane oxalate transport in human red blood cells in idiopathic calcium oxalate nephrolithiasis. Gambaro et al. (J Am Soc Nephrot, 7(4):608, 1996) described the predictive value of this erythrocyte anomaly for recurrent calcium oxalate nephrolithiasis. We examined the diagnostic validity of pH-value depended changes of the erythrocyte oxalate transport in calciurn oxalate stone formers vs. normal controls. MATERIAL & METHODS: We studied 2 groups of 10 normal controls (CG) and 10 idiopathic CaOx stone formers (CaOxG). The mean age in the normal control was 58.6 (+14.8) years and in the stone formers 58.4 (±14.1) years. Blood was drawn into a 10 ml ammoniac heparinat monovette, the red cells were isolated and resuspended to pH-value 7.70; pH-value 7.74 and pH-value 8.4 buffered solution. 0.1 mmol/1 oxalate was added to each solution. The supernatant immediately and one hour after incubation was mixed with 15% HCI solution for oxalate measurement by chemiluminescence as described by Albrecht et. al. (J. Biolumin Chemilumin 8:21, 1993). The mean oxalate concentrations immediately after addition of oxalate and one hour after incubation were significantly different in the CaOx group 1.57 ~:0.7 rag/1 vs. 1.5 4-0.7 mg/1 (p< 0.02) and the normal control group and 2.09 +0.58 mg/1 vs. 2.14 4-0.63 mg/1 (p< 0.05). The mean intraerythrocyte oxalate flux was 19.27 ±7.3 ng/l*s for CaOXG and -13.73 4-16.9 ng/l*s for NG (io < 0.01). The mean intraerythrocyte oxalate flux was significantly reduced by higher pHvalues 19.27 ±7.3 ng/l*s vs. 2.39 4-24.7 ng]l*s (pi<0.05) and increased by lower pH-values 19.27 4-7.3 ng/l*s vs. 20.08 ±16.9 ng/l*s (n.s.) in the CaOX group. The mean intraerythrocyte oxalate flux in normal controls -13.73 ~:16.9 ng/l*s was increased both by lower pH-values -1.94 4-19.1 ng/l*s (pl<0.05) and higher pH-values -2.07 4-23.4 ng/l*s (n.s.) CONCLUSIONS: The human erythrocyte oxalate transport of idiopathic CaOx stone formers and normal controls had significantly inverse effects. The intraerythrocyte oxalate flux in idiopathic CaOx stone formers can be reduced by higher pH-values.
Syndrome of Inappropriate Secretion of Antidiuretic Hormone due to Malignant Thymoma
Nephron, 2002
Wiener klinische Wochenschrift, 2008
Zusammenfassung. Die xanthogranulomatöse Epididymitis (XE) ist ein seltener nicht-neoplastischer ... more Zusammenfassung. Die xanthogranulomatöse Epididymitis (XE) ist ein seltener nicht-neoplastischer Prozess mit Zerstörung von Gewebe, welches durch eine eindrucksvolle zelluläre Infiltration mit schaumigen Makrophagen, dichten Lymphozyten und Plasmazellen ersetzt wird. Wir berichten über einen 72-jährigen Mann mit der klinischen Anamnese einer inadäquat behandelten arteriellen Hypertonie, der bei uns mit einer seit 10 Tagen bestehenden schmerzhaften Tumormasse rechts im Bereich des Skrotums vorstellig wurde. Bei der physikalischen Untersuchung wurde ein Eiter sezernierendes, hyperämisiertes und ödematöses Skrotum bei normaler Körpertemperatur festgestellt. Die Tumormarker für maligne Hodenerkrankungen waren negativ. Die Ultraschalluntersuchung (US) des rechten Hodens zeigte ein ödematös geschichtetes Skrotum sowie ein heterogenes Areal mit schlecht definierter Abgrenzung im Hoden und Nebenhoden. Es bestand eine minimale Hydrocele, der rechte Funikulus hatte einen normalen Durchmesser ohne Ödem oder andere pathologische Formationen. Die Progression der klinischen Befunde, der US und auch der Farb-US zusammen mit den negativen Tumormarkern ergaben letztlich die Indikation zur chirurgischen Sanierung. Es wurde eine Orchiepididymektomie rechts nach entsprechender präoperativer Therapie durchgeführt. Die Histologie bestätigte die Diagnose einer XE. Summary. Xanthogranulomatous epididymitis is an uncommon non-neoplastic process with destruction of tissue and replacement by striking cellular infiltration of foamy macrophages, dense lymphocytes and plasma cells. We report on a 72-year-old man with a clinical his-tory of inadequately treated arterial hypertension, who presented with a right scrotal mass associated with right scrotal pain for 10 days. Physical examination revealed pyogenic discharge from the hyperemic and edematous scrotum, with normal body temperature. Testicular tumor markers were normal. Ultrasonography (US) of the right testis showed edematous scrotal layers and a heterogeneous area of poorly defined margins within the testis and epididymis. There was minimal hydrocele, and the right funiculus was of normal diameter with no edema or pathologic formation. The progression of clinical findings, inflammatory parameters, US and color Doppler US findings with negative testicular tumor markers indicated surgical treatment. After preoperative treatment, right orchiepididymectomy was performed. Histology confirmed the diagnosis of xanthogranulomatous epididymitis.
Cytotoxic and apoptotic effects of fumonisin B1, beauvericin and ochratoxin A on porcine kidney PK-15 cells
Toxicology Letters, 2006
Fumonisin B1 (FB1), beauvericin (BEA) and ochratoxin A (OTA) are widespread mycotoxins, which con... more Fumonisin B1 (FB1), beauvericin (BEA) and ochratoxin A (OTA) are widespread mycotoxins, which contaminate food and feed, particularly maize. Their co-occurrence was established in 6% (two toxins) and 2% (three toxins) of maize samples in Croatia with average concentrations 0.62 mg/kg of FB1, 0.39 mg/kg of BEA and 0.02 mg/kg of OTA. Fumonisin B1 and OTA could be implicated in development of various diseases in animals and humans, including nephrotoxicosis and carcinogenesis. Cytotoxic and apoptotic activity of BEA was observed in few cell lines including human B-lymphocytes, rodent colangiocytes and human cell lines of myeloid origin. However, possible nephrotoxic effects of BEA have not been investigated yet. In this study we explored cytotoxic (cell viability) and apoptotic (index of apoptosis and activation of caspase-3) effects of FB1, BEA and OTA in PK-15 cells (porcine kidney epithelial cells). Cells were treated with lower concentrations of mycotoxins (0.05, 0.5 and 5 g/mL) for 24 and 48 hours. Decrease of cell viability shows that FB1, BEA and OTA are cytotoxic to PK-15 cells in dose dependent manner. Cell viability was significantly decreased after 24 hours of exposure to 5 g/mL of FB1 (25%), BEA (30%) and OTA (40%), as compared to untreated control cells (P<0.05). On the other hand, index of apoptosis was increased by 110-140% after 48 hours of exposure to individual mycotoxins (5 g/mL), as compared to control samples (P<0.05). OTA activated caspase-3 after 24 hours of treatment with 0.5 g/mL (84%), while BEA (319%) and FB1 (419%) significantly affected this enzyme after prolonged exposure (P<0.05). Combined treatment with two or three mycotoxins (0.05 and 0.5 g/mL) showed additive effects on cell viability. Beauvericin and OTA (5 g/mL) synergistically increased apoptotic index after 24 hours of treatment. Synergistic effects on caspase-3 activities were observed after 24 hours of exposure to BEA+OTA and FB1+BEA+OTA (0.05 and 0.5 g/mL), as well as to FB1+BEA and BEA+OTA (5 g/mL). From the mycotoxicological risk point of view, frequent maize contamination by fungal species that produce FB1 and BEA (Fusarum spp.), as well as OTA (Aspergillus spp. and Penicillium spp.), is one of the greatest concern. Co-occurrence and accumulation of these mycotoxins in grains and their synergistic action might be an important trigger for development of chronic renal diseases, especially after long-term exposure of consumers.
The effect of single dose of fumonisin B1 on oxidative stress, sphingolipid metabolism and DNA damage in rat kidney
Toxicology Letters, 2007
End-Stage Kidney Disease After Mushroom Poisoning and Abo-Incompatible Liver Transplantation
Nephrology, 2010
10 months after her admission, the patient is free from any sequelae of HUS. Ito et al. recently ... more 10 months after her admission, the patient is free from any sequelae of HUS. Ito et al. recently reported the efficacy of IVIG for thrombotic microangiopathy of unknown aetiology. Our patient experienced a significant and rapid improvement, especially of severe CNS signs following IVIG as well as favourable MRI changes, although the precise mechanism of this therapy remains speculative. However, we believe that both supportive therapy and anti-pro-inflammatory cytokine therapy should be considered for selected patients with HUS and CNS involvement caused by endothelial microangiopathy.
Nephrology, 2008
Instructive Cases MINIMAL CHANGE DISEASE AND ACUTE TUBULAR NECROSIS CAUSED BY DICLOFENAC Renal si... more Instructive Cases MINIMAL CHANGE DISEASE AND ACUTE TUBULAR NECROSIS CAUSED BY DICLOFENAC Renal side-effects of non-steroidal anti-inflammatory drugs (NSAID) can be divided into five clinical syndromes: (i) acute renal failure; (ii) acute interstitial nephritis with nephrotic syndrome; (iii) electrolyte and fluid disorders; (iv) hypertension; and (v) analgesic nephropathy. 1 We describe an unusual combination of minimal change disease (MCD) and acute tubular necrosis (ATN) caused by diclofenac. A 53-year-old woman with nephrotic syndrome and acute renal failure was admitted to our hospital. The patient's medical history was unremarkable except for nontreated hypertension for 5 years. The only medication was oral diclofenac for chronic muscular pain and knee arthropathy. The patient was pale, 83 kg of weight, with generalized oedema and blood pressure 130/80 mmHg. Ocular fundus showed hypertensive changes grade I/II. Erythrocyte sedimentation rate was 21 mm/h and white blood cells count 9 ¥ 10 9 /L without eosinophilia. Urine dipstick showed +++ protein. There were a few erythrocytes and leucocytes in the urine sediment, but no eosinophils. Protein urine excretion was 6.0 g/day and creatinine was 716 mmol/L. Total serum protein was 59 g/L and albumin 23 g/L. Light microscopy renal biopsy showed glomeruli with normal morphology. There was severe tubular injury (Fig. ). Arterioles showed mild to moderate hyaline arte-
Journal of Physiology-Paris, 1997
Besides a superior protection of the pentadecapeptide BPC 157 (an essential fragment of an organo... more Besides a superior protection of the pentadecapeptide BPC 157 (an essential fragment of an organoprotective gastric juice peptide BPC) against different gastrointestinal and liver lesions. an acute anti-inflammatory and analgetic activity was also noted. Consequently. its effect on chronic inflammation lesions, such as adjuvant arthritis. and non-steroidal anti-inflammatory agents (NSAIAs)-induced gastrointestinal lesions was simultaneously studied in rats. In gastrointestinal lesions (indomethacin (30 mg/kg SC). aspirin (400 mg/kg ig) and diclofenac (125 mg/kg ip) studies, BPC 157 (10 pg or 10 ng/kg ip) was regularly given simultaneously and/or 1 h prior to drug application (indomethacin). In the adjuvant arthritis (tail-application of 0.2 mL of Freund's adjuvant) studies (14 days, 30 days, 1 year) BPC 157 (10 pg or 10 ng/kg ip). it was given as a single application (at 1 h either before or following the application of Freund's adjuvant) or in a once daily regimen (O-14th day, 1430th day, 14th day-l year). Given with the investigated NSAIAs, BPC 157 consistently reduced the otherwise prominent lesions in the stomach of the control rats, as well as the lesions in the small intestine in the indomethacin groups. In the adjuvant arthritis studies, the lesion's development seems to be considerably reduced after single pentadecapeptide medication. and even more attenuated in rats daily treated with BPC 157. As a therapy of already established adjuvant arthritis, its salutary effect consistently appeared already after 2 weeks of medication and it could be clearly seen also after 1 year of application. Taking together all these results, the data likely point to a special anti-inflammatory and mucosal integrity protective effect. pentadecapeptide BPC 157 I peptide BPC / gastrointestinal lesions / adjuvant arthritis
International Urology and Nephrology, 2013
PURPOSE: There is a paucity of epidemiological data on biopsy-proven renal disease in Croatia. Th... more PURPOSE: There is a paucity of epidemiological data on biopsy-proven renal disease in Croatia. The purpose of this report is a review of clinical and histological data, over a period of 15 years, from the single biggest adult native renal biopsy centre in Croatia. METHODS: This report includes data from 922 adult native renal biopsies in patients referred from the whole country and performed in our centre from 1996 till February 2012. Data on age, gender, serum creatinine, urine sediment, 24-hour proteinuria, clinical syndrome and histological diagnosis were collected and analyzed retrospectively. In all patients light, immunofluorescence and electron microscopic analysis was performed. The median age of the patients was 48 years (interquartile range 36-59 years), and the majority of patients were men (57.8%). The most common indication for renal biopsy was nephrotic syndrome (40.3%) followed by asymptomatic urinary abnormalities (31.7%). The most common biopsy-proven renal disease in total was IgA glomerulonephritis (19.3%), followed by FSGS (15.8%) and membranous glomerulonephritis (9.2%). In men similar results were found, while in women the most common were hereditary nephritis (13.4%), FSGS (12.9%) and connective tissue disease-related glomerular disorders (11.6%). CONCLUSION: The presented data are an important contribution to the better understanding of the epidemiology of biopsy-proven renal disease in Croatia and Europe throughout comparison with other registry data. This data should be the basis for the formation of Croatian Registry of Renal Biopsies.
Beneficial effect of a novel pentadecapeptide BPC 157 on gastric lesions induced by restraint stress, ethanol, indomethacin, and capsaicin neurotoxicity
Digestive Diseases and Sciences, 1996
Very recently, the integrity of capsaicin somatosensory neurons and their protection were suggest... more Very recently, the integrity of capsaicin somatosensory neurons and their protection were suggested to be related to the activity in nociception of a newly discovered 15-amino acid peptide, BPC 157, shown to have strong beneficial effect on intestinal and liver lesions. Therefore, from this viewpoint, we have studied the gastroprotective effect of the pentadecapeptide BPC 157, on gastric lesions produced in rats by 96% ethanol, restraint stress, and indomethacin. The possible involvement of sensory neurons in the salutary actions of BPC 157 (10 micrograms/kg, 10 ng/kg intraperitoneally) was studied with capsaicin, which has differential effects on sensory neurons: a high dose in adult (125 mg/kg subcutaneously, 3 months old) or administration (50 mg/kg subcutaneously) to neonatal animals (age of the 7 days) destroys sensory fibers, whereas a low dose (500 micrograms/kg intraperitoneally) activates neurotransmitter release and protective effects on the mucosa. In the absence of capsaicin, BPC 157 protected gastric mucosa against ethanol, restraint, and indomethacin application. In the presence of neurotoxic doses of capsaicin, the negative influence of capsaicin on restraint, ethanol, or indomethacin lesions consistently affected salutary activity of BPC 157. However, BPC 157 protection was still evident in the capsaicin-treated rats (either treated as adults or as newborns) in all of these assays. Interestingly, after neonatal capsaicin treatment, a complete abolition of BPC gastroprotection was noted if BPC 157 was applied as a single nanogram-regimen, but the mucosal protection was fully reversed when the same dose was used daily. In line with the excitatory dose of capsaicin the beneficial effectiveness of BPC 157 appears to be increased as well. Taken together, these data provide evidence for complex synergistic interaction between the beneficial effectiveness of BPC 157 and peptidergic sensory afferent neuron activity.
Digestive Diseases and Sciences, 1996
The superior effectiveness of a new pentadecapeptide, BPC 157, on gastrointestinal and liver lesi... more The superior effectiveness of a new pentadecapeptide, BPC 157, on gastrointestinal and liver lesions, in conjunction with an antiinflammatory and analgetic activity was recently noted. In the present study, BPC 157 was tested as either a protective or healing agent in bile duct ligationinduced acute pancreatitis in rats. In addition, the positive influence of BPC 157 on concomitantly developed gastric and duodenal lesions was simultaneously investigated. BPC 157 (10 p.g, 10 ng/kg body wt, intraperitoneally or intragastrically) was given prophylactically 1 hr before ligation, whereas the therapy was given once daily beginning with the 24 hr following ligation (last application 24 hr before killing). The effect was investigated at daily intervals until the end of the frith day after ligation. In the pretreatment regimen, a strong pancreas protection was obtained. When applied in the condition of already established severe acute pancreatitis, an obvious salutary effect was consistently noted. Assessing the appearance of the necrosis, edema, neutrophils, and mononuclears, consistently less necrosis, edema, and neutrophils, but more mononuclears, were found in BPC-treated rats. Likewise, in studies of the serum amylase values, relative to control data, a markedly lower rise (BPC pretreatment regimen) as well as a worsening of the already raised values (BPC therapy regimen) was noted. Along with its beneficial effect on pancreatitis, a positive influence of BPC 157 on the gastric and duodenal lesion course in bile duct-ligated rats was noted in both the pre-and posttreatment regimen. Taken together, in further studies of acute pancreatitis therapy, BPC could be an interesting and useful agent with an additional positive impact on concomitant gastroduodenal pathology.
Cytotoxicity and apoptosis induced by fumonisin B1, beauvericin and ochratoxin A in porcine kidney PK15 cells: effects of individual and combined treatment
Archives of Toxicology, 2007
The objective of this study was to determine individual and combined effects of fumonisin B(1) (F... more The objective of this study was to determine individual and combined effects of fumonisin B(1) (FB(1)), beauvericin (BEA) and ochratoxin A (OTA) on porcine kidney epithelial PK15 cell survival by measuring lactate dehydrogenase (LDH) activity, apoptotic index and caspase-3 activity. Cells were treated with 0.05, 0.5 and 5 microg/ml of each mycotoxin or with the combinations of two or all three mycotoxins for 24 and 48 h. Changes in LDH and caspase-3 activity, and in apoptotic index showed that the cytotoxic and apoptotic effects of these mycotoxins were concentration- and time- dependent. Significant increase of LDH activity was observed after 48 h of exposure to the highest concentration of FB(1) (45%), BEA (84%) and OTA (77%), as compared to control. OTA increased caspase-3 activity after 24 h of treatment with 0.5 mug/mL (84%), while BEA (319%) and FB(1) (419%) significantly affected this enzyme activity after 48 h (P &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt; 0.05). Increase of caspase-3 activity preceded significant morphological apoptotic changes, which were detected after 48 h of exposure to a single toxin. Combined treatment with FB(1), BEA and OTA resulted mostly in additive effects on LDH activity, and additive and synergistic effects on caspase-3 activity and apoptotic index.
Pentadecapeptide BPC 157 interactions with adrenergic and dopaminergic systems in mucosal protection in stress
Digestive diseases and …, 1997
Since superior protection against different gastrointestinal and liver lesions and antiinflammato... more Since superior protection against different gastrointestinal and liver lesions and antiinflammatory and analgesic activities were noted for pentadecapeptide BPC (an essential fragment of an organoprotective gastric juice protein named BPC), the beneficial mechanism of BPC 157 and its likely interactions with other systems were studied. Hence its beneficial effects would be abolished by adrenal gland medullectomy, the influence of different agents affecting alpha, beta, and dopamine receptors on BPC 157 gastroprotection in 48 h restraint stress was further investigated. Animals were pretreated (1 hr before stress) with saline (controls) or BPC 157 (dissolved in saline) (10 microg or 10 ng/kg body wt intraperitoneally or intragastrically) applied either alone to establish basal conditions or, when manipulating the adrenergic or dopaminergic system, a simultaneous administration was carried out with various agents with specific effects on adrenergic or dopaminergic receptors [given in milligrams per kilogram intraperitoneally except for atenolol, which was given subcutaneously] phentolamine (10.0), prazosin (0.5), yohimbine (5.0), clonidine (0.1) (alpha-adrenergic domain), propranolol (1.0), atenolol (20.0) (beta-adrenergic domain), domperidone (5.0), and haloperidol (5.0) (peripheral/central dopamine system). Alternatively, agents stimulating adrenergic or dopaminergic systems--adrenaline (5.0) or bromocriptine (10.0)--were applied. A strong protection, noted following intragastric or intraperitoneal administration of BPC 157, was fully abolished by coadministration of phentolamine, clonidine, and haloperidol, and consistently not affected by prazosin, yohimbine, or domperidone. Atenolol abolished only intraperitoneal BPC 157 protection, whereas propranolol affected specifically intragastric BPC 157 protection. Interestingly, the severe course of lesion development obtained in basal conditions, unlike BPC 157 gastroprotection, was not influenced by the application of these agents. In other experiments, when adrenaline and bromocriptine were given simultaneously, a strong reduction of lesion development was noted. However, when applied separately, only adrenaline, not bromocriptine, has a protective effect. Thus, a complex protective interaction with both alpha-adrenergic (eg, catecholamine release) and dopaminergic (central) systems could be suggested for both intragastric and intraperitoneal BPC 157 administration. The involvement of beta-receptor stimulation in BPC 157 gastroprotection appears to be related to the route of BPC 157 administration. The demonstration that a combined stimulation of adrenergic and dopaminergic systems by simultaneous prophylactic application of adrenaline (alpha- and beta-receptor stimulant) and bromocriptine (dopamine receptor agonist) may significantly reduce restraint stress lesions development provides insight for further research on the beneficial mechanism of BPC 157.
Journal of the American Society of Nephrology, 2008
IL-33 deficiency slows cancer growth but does not protect against cisplatin-induced AKI in mice with cancer
American journal of physiology. Renal physiology, Jan 25, 2017
The effect of IL-33 deficiency on AKI and cancer growth in a 4 week model of cisplatin-induced AK... more The effect of IL-33 deficiency on AKI and cancer growth in a 4 week model of cisplatin-induced AKI in mice with cancer was determined. Mice were injected subcutaneously with murine lung cancer cells. Ten days later, cisplatin (10 mg/kg/week) was administered weekly for 4 weeks. The increase in kidney IL-33 preceded the AKI and tubular injury suggesting that IL-33 may play a causative role. However, the increase in serum creatinine, BUN, serum NGAL, ATN and apoptosis scores in the kidney in cisplatin-induced AKI was the same in wild type and IL-33 deficient mice. There was an increase in kidney expression of pro-inflammatory cytokines CXCL1 and TNF-α, known mediators of cisplatin-induced AKI, in IL-33 deficient mice. Surprisingly, tumor weight, tumor volume and tumor growth were significantly decreased in IL-33 deficient mice and the effect of cisplatin on tumors was enhanced in IL-33 deficient mice. As serum IL-33 was increased in cisplatin-induced AKI in mice, it was determined whe...
Croatica Chemica Acta, Apr 15, 2008
It is known that exposure to mycotoxin fumonisin B 1 (FB 1) causes apoptosis and increases lipid ... more It is known that exposure to mycotoxin fumonisin B 1 (FB 1) causes apoptosis and increases lipid peroxidation in the kidney of experimental animals. In this study, adult male Wistar rats were given a single dose (5, 50, 500 mg per kg of body mass) by gavage and sacrificed 4, 24 and 48 hours after dosing. The parameters of oxidative stress, sphingolipids, DNA lesions and histopathological changes were checked in the kidney of treated and control animals. The metabolism of sphingolipids was significantly affected with the lowest FB 1 dose (sphinganine/sphingosine: 2.53 ± 0.52 vs. control 0.62 ± 0.10), while even the highest dose did not change the parameters of oxidative stress. DNA lesions measured as comet tail length (range (13.6 ± 0.41) mm to (19.83 ± 1.43) mm) and tail intensity ((0.60 ± 0.16) % to (2.74 ± 0.17) %) were FB 1 dose-and time-dependent. Apoptotic cells and mitotic figures were found in the cortical and outer stripe of the medullar part of the kidney.
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Papers by Danica Ljubanović