The present research deals with the comparative analysis of Anti-Microbial and Phytochemical prop... more The present research deals with the comparative analysis of Anti-Microbial and Phytochemical properties of Piper longum and Piper nigrum. Six isolates of microorganisms were used to check the antimicrobial properties. Agar well diffusion method and Agar disc diffusion method was employed. The volatile oil content was absent in both plant materials. Water extractive value for P. nigrum was about 7.6% and P. longum was 12%.alcohol soluble extractive value for P. nigrum was only 2% and P. longum was found to be 7.6%. Both plant species are rich in phytochemical constituents such as alkaloids, steroids, flavonoids etc. The present result suggests that alcoholic extract of P. nigrum is a curing agent for diseases caused by E. coli.
The identification of L-Asparaginase producing fungal organisms has gained considerable attention... more The identification of L-Asparaginase producing fungal organisms has gained considerable attention because of its less allergic properties. The present investigation deals with the morphological and molecular identification of L-Asparaginase producing fungi from the leaves of the medicinal plant A. muricata. It was confirmed that the fungal isolate obtained from A. muricata has the potential to produceAsparaginase and the fungal species was identified as Aspergillus fumigatus.
The present research describes the production and characterization of L-Asparaginase from Aspergi... more The present research describes the production and characterization of L-Asparaginase from Aspergillus fumigatus isolated from the medicinal plant Annona muricata. This fungal isolate have shown potential to produce L-Asparaginase on modified Czapek Dox medium under optimal conditions. Characterization of enzyme was revealed the finest conditions for highest production.it was observed that the maximum production of L-Asparaginase at a pH of 8, 40 C of temperature, 20 minutes of incubation period, Presence of Fe ions and. 20mM substrate concentration.
The present investigation deals with the anti-proliferative activity of an extracellular enzyme k... more The present investigation deals with the anti-proliferative activity of an extracellular enzyme known as LAsparaginase from Aspergillus fumigatus screened from infected leaves of the medicinal plant Annona muricata. The anti-proliferative activity of L-Asparaginase from Aspergillus fumigatus was checked on human breast cancer cell line MDA-MB-231.The influence of pH, temperature, substrate concentration, incubation period and concentration of metal ions were analyzed in the production of L-Asparaginase. The highest production of enzyme noticed at a pH of 8, 20mM substrate concentration, 20 minutes of incubation period, Presence of Fe ions and 40 C of temperature. These were the optimum conditions for the effective yield of enzyme. The enzyme isolated from Aspergillus fumigatus is a promising antiproliferative agent and can be used in the expansion of further compositions for the therapy of tumors.
The present research deals with the comparative analysis of Anti-Microbial and Phytochemical prop... more The present research deals with the comparative analysis of Anti-Microbial and Phytochemical properties of Piper longum and Piper nigrum. Six isolates of microorganisms were used to check the antimicrobial properties. Agar well diffusion method and Agar disc diffusion method was employed. The volatile oil content was absent in both plant materials. Water extractive value for P. nigrum was about 7.6% and P. longum was 12%.alcohol soluble extractive value for P. nigrum was only 2% and P. longum was found to be 7.6%. Both plant species are rich in phytochemical constituents such as alkaloids, steroids, flavonoids etc. The present result suggests that alcoholic extract of P. nigrum is a curing agent for diseases caused by E. coli.
The identification of L-Asparaginase producing fungal organisms has gained considerable attention... more The identification of L-Asparaginase producing fungal organisms has gained considerable attention because of its less allergic properties. The present investigation deals with the morphological and molecular identification of L-Asparaginase producing fungi from the leaves of the medicinal plant A. muricata. It was confirmed that the fungal isolate obtained from A. muricata has the potential to produceAsparaginase and the fungal species was identified as Aspergillus fumigatus.
The present research describes the production and characterization of L-Asparaginase from Aspergi... more The present research describes the production and characterization of L-Asparaginase from Aspergillus fumigatus isolated from the medicinal plant Annona muricata. This fungal isolate have shown potential to produce L-Asparaginase on modified Czapek Dox medium under optimal conditions. Characterization of enzyme was revealed the finest conditions for highest production.it was observed that the maximum production of L-Asparaginase at a pH of 8, 40 C of temperature, 20 minutes of incubation period, Presence of Fe ions and. 20mM substrate concentration.
The present investigation deals with the anti-proliferative activity of an extracellular enzyme k... more The present investigation deals with the anti-proliferative activity of an extracellular enzyme known as LAsparaginase from Aspergillus fumigatus screened from infected leaves of the medicinal plant Annona muricata. The anti-proliferative activity of L-Asparaginase from Aspergillus fumigatus was checked on human breast cancer cell line MDA-MB-231.The influence of pH, temperature, substrate concentration, incubation period and concentration of metal ions were analyzed in the production of L-Asparaginase. The highest production of enzyme noticed at a pH of 8, 20mM substrate concentration, 20 minutes of incubation period, Presence of Fe ions and 40 C of temperature. These were the optimum conditions for the effective yield of enzyme. The enzyme isolated from Aspergillus fumigatus is a promising antiproliferative agent and can be used in the expansion of further compositions for the therapy of tumors.
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