Papers by Cristina Sabliov
Typescript. Thesis (M.S.)--University of Missouri-Columbia, 1998. Includes bibliographical refere... more Typescript. Thesis (M.S.)--University of Missouri-Columbia, 1998. Includes bibliographical references (leaves 107-110). Microfilm.
This project aims to increase bioavailability and bioaccessibility of lipophilic vitamins in the ... more This project aims to increase bioavailability and bioaccessibility of lipophilic vitamins in the human body by entrapping the vitamins in mucoadhesive cationic poly (D, L Lactideco glycolide) (PLGA)/chitosan particles. Entrapping the bioactive vitamins in a lecithin stabilized polymeric matrix, of well characterized release kinetics, ensures that the vitamin is released from the matrix and becomes available for absorption, thus, increasing its bioaccessibility. Chitosan induces particle attachment to mucus tissue (mucoadhesion) and increases retention time of vitamins in the gastrointestinal tract, close to the absorptive cells to ensure an increase in the bioavailability. Mucoadhesive particle systems are desired for oral delivery because they decrease translocation of the particles into the cells by elimination of the particles during mucus turnover. This will ensure reduced toxicity from byproducts of degradation. For added safety, the major reagents used in the synthesis are...
Journal of Nanomaterials, 2011
Poly(D,L-lactide-co-glycolide-) (PLGA-)chitosan nanoparticles are becoming an increasingly common... more Poly(D,L-lactide-co-glycolide-) (PLGA-)chitosan nanoparticles are becoming an increasingly common choice for the delivery of nucleic acids to cells for various genetic manipulation techniques. These particles are biocompatible, with tunable size and surface properties, possessing an overall positive charge that promotes complex formation with negatively charged nucleic acids. This study examines properties of the PLGA-chitosan nanoparticle/plasmid DNA complex after formation. Specifically, the study aims to determine the optimal ratio of plasmid DNA:nanoparticles for nucleic acid delivery purposes and to elucidate the location of the pDNA within these complexes. Such characterization will be necessary for the adoption of these formulations in a clinical setting. The ability of PLGA-chitosan nanoparticles to form complexes with pDNA was evaluated by using the fluorescent intercalating due OliGreen to label free plasmid DNA. By monitoring the fluorescence at different plasmid: nanoparticle ratios, the ideal plasmid:nanoparticle ration for complete complexation of plasmid was determined to be 1:50. Surface-Enhanced Raman Spectroscopy and gel digest studies suggested that even at these optimal complexation ratios, a portion of the plasmid DNA was located on the outer complex surface. This knowledge will facilitate future investigations into the functionality of the system in vitro and in vivo.
Background T he effects of extraction parameters, ex-traction temperature and extraction time wer... more Background T he effects of extraction parameters, ex-traction temperature and extraction time were assessed for microwave ex-traction of rice bran oil. The objectives of the research were: to effectively extract rice bran oil from rice bran using microwave as-sisted extraction and to analyze the influence of temperature and extraction time on the rice bran oil and vitamin E yield. Results showed that the extraction time had a minimal effect on the vitamin E and rice bran oil yield at all temperatures. Temperature, on the other hand, had a significant effect on the oil and vitamin E yield. More vitamin E was extracted at 140°C (P less than 0.05.
The Journal of microwave power and electromagnetic energy: a publication of the International Microwave Power Institute
Continuous microwave heating has the potential to revolutionize processing operations in which hi... more Continuous microwave heating has the potential to revolutionize processing operations in which high energy densities per unit volume are required. The adoption of microwave heating for large scale commercial application has been hampered by a lack of reliable information on the temperature distribution during heating of pumpable fluids. This study represents an initial attempt to design a system that quantifies the temperature distribution during continuous microwave heating and its relationship with processing tube surface temperature distribution. The novel measurement system designed consisted of a semi-rigid thin axial support system on which fiber optic probes were attached at different lengths in the microwave heating cavity. Inlet, outlet and in-line longitudinal temperatures were monitored during continuous flow microwave heating (1.5 kW, 2.5 kW, 3.5 kW, and 4.5 kW) of water of three different salinities (0 ppt, 15 ppt, and 30 ppt) at a flow rate of 1.6 l/min. Inlet and exit...
The Journal of microwave power and electromagnetic energy: a publication of the International Microwave Power Institute
Numerical models were developed to simulate temperature profiles in Newtonian fluids during conti... more Numerical models were developed to simulate temperature profiles in Newtonian fluids during continuous flow microwave heating by one way coupling electromagnetism, fluid flow, and heat transport in ANSYS 8.0 and COMSOL Multiphysics v3.4. Comparison of the results from the COMSOL model with the results from a pre-developed and validated ANSYS model ensured accuracy of the COMSOL model. Prediction of power Loss by both models was in close agreement (5-13% variation) and the predicted temperature profiles were similar. COMSOL provided a flexible model setup whereas ANSYS required coupling incompatible elements to transfer load between electromagnetic, fluid flow, and heat transport modules. Overall, both software packages provided the ability to solve multiphysics phenomena accurately.
Objective: Over the last decade, the study of nanoparticle interaction with various human tissues... more Objective: Over the last decade, the study of nanoparticle interaction with various human tissues and cell types has became an interdisciplinary research field of great interest. Using biodegradable polymeric nanoparticles for biomedical applications offers several advantages such as: biocompatibility, biodegradability coupled with minimum side effects. Oral route represents one of the proposed systems for nanoparticle delivery. However, data on nanoparticle interaction with oral mucosa and its cells, is insufficient. In this context the present work is focusing on the uptake of poly(lactic-co-glycolic acid) PLGA nanoparticles in oral keratinocyte stem cells. Method: Oral keratinocyte stem cells with the phenotype α6β4 pos CD71neg were separated from human oral mucosa using a magnetic based method. The cells were exposed to different concentrations of PLGA and PLGA-Chitosan nanoparticles. The cells were further analyzed at different time points for uptake, proliferation, and cytotox...
Food Chemistry, 2015
The aim of the study was to evaluate the ability of poly(lactic-co-glycolic acid) (PLGA) nanopart... more The aim of the study was to evaluate the ability of poly(lactic-co-glycolic acid) (PLGA) nanoparticles (NP) to enhance lutein bioavailability. The bioavailability of free lutein and PLGA-NP lutein in rats was assessed by determining plasma pharmacokinetics and deposition in selected tissues. Lutein uptake and secretion was also assessed in Caco-2 cells. Compared to free lutein, PLGA-NP increased the maximal plasma concentration (Cmax) and area under the time-concentration curve in rats by 54.5- and 77.6-fold, respectively, while promoting tissue accumulation in the mesenteric fat and spleen. In comparison with micellized lutein, PLGA-NP lutein improved the Cmax in rat plasma by 15.6-fold and in selected tissues by ⩾3.8-fold. In contrast, PLGA-NP lutein had a lower uptake and secretion of lutein in Caco-2 cells by 10.0- and 50.5-fold, respectively, compared to micellized lutein. In conclusion, delivery of lutein with polymeric NP may be an approach to improve the bioavailability of lutein in vivo.
Industrial Biotechnology, 2013
Annual review of food science and technology, 2014
Food bioactives are known to prevent aging, cancer, and other diseases for an overall improved he... more Food bioactives are known to prevent aging, cancer, and other diseases for an overall improved health of the consumer. Nanodelivery provides a means to control stability, solubility, and bioavailability, and also provides controlled release of food bioactives. There are two main types of nanodelivery systems, liquid and solid. Liquid nanodelivery systems include nanoemulsions, nanoliposomes, and nanopolymersomes. Solid nanodelivery systems include nanocrystals, lipid nanoparticles, and polymeric nanoparticles. Each type of nanodelivery system offers distinct benefits depending on the compatibility of nanoparticle properties with the properties of the bioactive and the desired application. Physicochemical properties of nanoparticles such as size, charge, hydrophobicity, and targeting molecules affect the absorption, distribution, metabolism, and excretion (ADME) of nanodelivery systems. The fate of the bioactive depends on its physicochemical properties and the location of its releas...
The Journal of microwave power and electromagnetic energy : a publication of the International Microwave Power Institute, 2007
The goal of this study was to numerically predict the temperature of a liquid product heated in a... more The goal of this study was to numerically predict the temperature of a liquid product heated in a continuous-flow focused microwave system by coupling high frequency electromagnetism, heat transfer, and fluid flow in ANSYS Multiphysics. The developed model was used to determine the temperature change in water processed in a 915 MHz microwave unit, under steady-state conditions. The influence of the flow rates on the temperature distribution in the liquid was assessed. Results showed that the average temperature of water increased from 25 degrees C to 34 degrees C at 2 l/min, and to 42 degrees C at 1 l/min. The highest temperature regions were found in the liquid near the center of the tube, followed by progressively lower temperature regions as the radial distance from the center increased, and finally followed by a slightly higher temperature region near the tube's wall corresponding to the energy distribution given by the Mathieu function. The energy distribution resulted in a...
Journal of nanoscience and nanotechnology, 2008
The utility of polymeric nanoparticles as drug delivery systems depends on effective control of s... more The utility of polymeric nanoparticles as drug delivery systems depends on effective control of synthetic parameters with a significant impact on their physico-chemical characteristics. In this study, a chemometric central composite experimental design (CCD) was used to optimize the synthesis of poly(D,L-lactide-co-glycolide) (PLGA) nanoparticles by emulsification solvent evaporation using anionic molecular micelles, such as poly(sodium N-undecylenic sulfate) (poly-SUS), poly(sodium N-undecanoyl-glycinate) (poly-SUG) and poly(sodium N-undecanoyl-L-leucyl-valinate) (poly-L-SULV) as well as conventional emulsifiers, such as anionic sodium dodecyl sulfate (SDS) and non-ionic poly(vinyl alcohol) (PVA). The individual and combined effects of PLGA concentration, emulsifier concentration, homogenization speed, and sonication time (design variables) on particle size and polydispersity index (responses) were investigated using multivariate analysis. The most significant design variables infl...
Nanotechnology Reviews, 2013
ABSTRACT
Journal of Chemistry, 2015
ABSTRACT Poly(lactic-co-glycolic acid) or PLGA is a biodegradable polymer used in a wide range of... more ABSTRACT Poly(lactic-co-glycolic acid) or PLGA is a biodegradable polymer used in a wide range of medical applications. Specifically PLGA materials are also developed for the dental field in the form of scaffolds, films, membranes, microparticles, or nanoparticles. PLGA membranes have been studied with promising results, either alone or combined with other materials in bone healing procedures. PLGA scaffolds have been used to regenerate damaged tissues together with stem cell-based therapy. There is solid evidence that the development of PLGA microparticles and nanoparticles may be beneficial to a wide range of dental fields such as endodontic therapy, dental caries, dental surgery, dental implants, or periodontology. The aim of the current paper was to review the recent advances in PLGA materials and their potential uses in the dental field.
Transactions of the ASABE, 2011
Control and Automation, 2011
2009 Reno, Nevada, June 21 - June 24, 2009, 2009
... The results showed that the lipid yield changes with temperature and achieves a maximum value... more ... The results showed that the lipid yield changes with temperature and achieves a maximum value in a relatively short time (order of minutes), with no yield increase at longer exposure times. ... Page 4. 4 Rice Bran CTT seeds Temperature (oC) Extraction time (min) ...
ABSTRACT Poly(lactic-co-glycolic acid) (PLGA) is a biodegradable polymer. Nanoparticles of PLGA a... more ABSTRACT Poly(lactic-co-glycolic acid) (PLGA) is a biodegradable polymer. Nanoparticles of PLGA are commonly used for drug delivery applications. The interaction of the nanoparticles with the cell membrane may influence the rate of their uptake by cells. Both PLGA and cell membranes are negatively charged, so adding positively charged polymers such as trimethyl chitosan (TMC) which adheres to the PLGA particles improves their cellular uptake. The interaction of 3 nm PLGA and TMC-modified-PLGA nanoparticles with lipid bilayers composed of mixtures of phosphatidylcholine and phosphatidylserine lipids was studied using molecular dynamics simulations. The free energy profiles as function of nanoparticles position along the normal direction to the bilayers were calculated, the distribution of phosphatidylserine lipids as a function of distance of the particle from the bilayer was calculated, and the time scale for particle motion in the directions parallel to the bilayer surface was estimated.
Journal of dairy science, 2015
Reducing particle size of salt to approximately 1.5 µm would increase its surface area, leading t... more Reducing particle size of salt to approximately 1.5 µm would increase its surface area, leading to increased dissolution rate in saliva and more efficient transfer of ions to taste buds, and hence, perhaps, a saltier perception of foods. This has a potential for reducing the salt level in surface-salted foods. Our objective was to develop a salt using a nano spray-drying method, to use the developed nano spray-dried salt in surface-salted cheese cracker manufacture, and to evaluate the microbiological and sensory characteristics of cheese crackers. Sodium chloride solution (3% wt/wt) was sprayed through a nano spray dryer. Particle sizes were determined by dynamic light scattering, and particle shapes were observed by scanning electron microscopy. Approximately 80% of the salt particles produced by the nano spray dryer, when drying a 3% (wt/wt) salt solution, were between 500 and 1,900 nm. Cheese cracker treatments consisted of 3 different salt sizes: regular salt with an average pa...
Abstract Text: Reducing the particle size of sodium chloride crystal would increase its dissoluti... more Abstract Text: Reducing the particle size of sodium chloride crystal would increase its dissolution rate leading to a more efficient transfer of the ions to the taste buds and hence perhaps a saltier perception of foods. The objective was to evaluate the effect of developed submicro salt on the sensory characteristics of surface salted cheese crackers. The cheese cracker treatments consisted of 3 different salt sizes (regular, micro, submicro salt) and 3 different concentrations (2, 1.5 and 1% w/w). A Balanced Incomplete Block Design was used to conduct the consumer analysis of cheese crackers for submicro salt (2, 1.5 and 1%), microsalt (2, 1.5 and1%) and regular 2% (control as used by industry) using 476 participants in total at week 1 and 4 months. At 4 months, submicro salt treatments (2, 1.5 and 1%) resulted in having significantly (P<0.05) more preferred saltiness scores compared to control (regular 2%). At 4 months, submicro salt (1.5 and 2%) showed significantly (P<0.0...
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Papers by Cristina Sabliov