Cratylia mollis is a natural forage plant from the Northeast of Brazil. C. mollis seed lectin (Cr... more Cratylia mollis is a natural forage plant from the Northeast of Brazil. C. mollis seed lectin (Cramoll) containing molecular forms 1 and 4 (Cramoll 1,4) has shown anti-inflammatory and wound-healing activities. This work analyzed the effect of Cramoll 1,4 on experimental schistosomiasis in mice. Experimental groups (n = 15/group) were composed of female albino Swiss mice, which were subcutaneously and caudally infected with Schistosoma mansoni (BH strain, 100 cercariae/mouse) and were treated with an intraperitoneal dose after infection as follows: (1) Cramoll 1,4 (50 mg kg−1 single dose – after 40 days of infection), (2) Cramoll 1,4 (7 mg kg−1 daily dose – for 7 days after infection) and control (untreated mice). Mice were sacrificed 8 weeks after infection and adult worms were recovered from the portal-hepatic system. Livers were fixed in 10% (v/v) formaldehyde/0.15 M NaCl and tissue sections were processed for haematoxilin and Masson's trichrome stainings. Mice infected subcutaneously harboured no or very few worms and hence the effect of Cramoll 1,4 could not be assessed. Results (P ≤ 0.05) were obtained with Cramoll 1,4 using the two treatments, with reduction of: egg excretion (79 and 80%), adult worm recovery (71 and 79%) and liver granulomas (40 and 73.5%) in relation to control. This study showed the potential anti-helminthic activity of Cramoll 1,4 when tested against Schistosomiasis mansoni infection in mice.The article investigates the anti-S. mansoni activity promoted by Cramoll 1,4 lectin isolated with high yield from Cratylia mollis Mart. seeds, a plant native to the Northeast of Brazil.► Cramoll 1,4 is a lectin extracted by seeds of Cratylia mollis plant. ► Their anti-schistosoma activity was investigated through in vivo assay. ► Cramoll promoted lower release of eggs, adult worms and granuloma in mice infected. ► Cramoll showed anti-S. mansoni activity.
Cratylia mollis is a natural forage plant from the Northeast of Brazil. C. mollis seed lectin (Cr... more Cratylia mollis is a natural forage plant from the Northeast of Brazil. C. mollis seed lectin (Cramoll) containing molecular forms 1 and 4 (Cramoll 1,4) has shown anti-inflammatory and wound-healing activities. This work analyzed the effect of Cramoll 1,4 on experimental schistosomiasis in mice. Experimental groups (n = 15/group) were composed of female albino Swiss mice, which were subcutaneously and caudally infected with Schistosoma mansoni (BH strain, 100 cercariae/mouse) and were treated with an intraperitoneal dose after infection as follows: (1) Cramoll 1,4 (50 mg kg−1 single dose – after 40 days of infection), (2) Cramoll 1,4 (7 mg kg−1 daily dose – for 7 days after infection) and control (untreated mice). Mice were sacrificed 8 weeks after infection and adult worms were recovered from the portal-hepatic system. Livers were fixed in 10% (v/v) formaldehyde/0.15 M NaCl and tissue sections were processed for haematoxilin and Masson's trichrome stainings. Mice infected subcutaneously harboured no or very few worms and hence the effect of Cramoll 1,4 could not be assessed. Results (P ≤ 0.05) were obtained with Cramoll 1,4 using the two treatments, with reduction of: egg excretion (79 and 80%), adult worm recovery (71 and 79%) and liver granulomas (40 and 73.5%) in relation to control. This study showed the potential anti-helminthic activity of Cramoll 1,4 when tested against Schistosomiasis mansoni infection in mice.The article investigates the anti-S. mansoni activity promoted by Cramoll 1,4 lectin isolated with high yield from Cratylia mollis Mart. seeds, a plant native to the Northeast of Brazil.► Cramoll 1,4 is a lectin extracted by seeds of Cratylia mollis plant. ► Their anti-schistosoma activity was investigated through in vivo assay. ► Cramoll promoted lower release of eggs, adult worms and granuloma in mice infected. ► Cramoll showed anti-S. mansoni activity.
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Papers by Cristiane Melo