This article is an open access article distributed under the terms and conditions of the Creative... more This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY
BackgroundBone marrow stem cells have been shown to be a promising therapeutic strategy for autoi... more BackgroundBone marrow stem cells have been shown to be a promising therapeutic strategy for autoimmune diseases. This study aimed to assess the safety and efficacy of autologous hematopoietic stem cell (ABMSC) transplantation without immunoablation used to suppress the autoimmune reaction in 6 children with newly diagnosed autoimmune diabetes mellitus. We monitored the levels of islet cell antibodies (ICA), antibodies against islet antigen-related tyrosine phosphatase 2 (IA2), glutamic acid-decarboxylase (GAD) antibodies, and anti-insulin antibodies (AIA).Material/MethodsBetween 2018 and 2022, 6 children (age 6–10 years, average 8 years) recently diagnosed with type 1 diabetes mellitus with the presence of ICA, IA2, GAD, AIA and ketoacidosis, were treated with an ABMSC stimulated with Filgrastim, granulocyte colony-stimulating factor (G-CSF), 10 ug/kg/day for 4 days. Bone marrow was harvested on day 5, collected by puncture and identified as mononuclear cells >180×106/kg, CD34+ >0.22%, and transplanted by intravenous (i.v.) infusion. Patients were monitored with ICA, IA2, GAD, AIA, C-peptide, blood glucose, and glycosylated hemoglobin A1c (HbA1C) 6 months after the procedure.ResultsAt 6-month follow-up, we observed a negative value of the ICA, which was previously positive (P<0.001). The IA2 (p=0.037) and GAD (P=0.377) antibodies decreased slowly but were significantly lower. AIA remained high. A decrease in blood glucose and HbA1C levels was observed (P<0.001). No complications occurred during follow-up.ConclusionsAutologous hematopoietic stem cell transplantation without immunoablation was safe and effective in significantly decreasing the production and effect of autoantibodies against ICA, GAD, and IA2, as well as decreasing blood sugar levels and HbA1c.
International journal of biomedical research & practice, Dec 31, 2022
Mitochondria are major organelles that produce energy for cellular metabolism and are vital to pr... more Mitochondria are major organelles that produce energy for cellular metabolism and are vital to proper cell functioning. Metabolic dysregulation is a critical contributing factor to the initiation and development of degenerative and autoimmune diseases, thus is present in cartilage disorders such as osteoarthritis and rheumatoid arthritis. Mitochondrial dysfunction in cartilage diseases is especially interesting because it may present a promising therapeutic and anti-ageing target. By understanding the mechanism of mitochondrial dysfunction, we can aim to upregulate the role of mitochondria in treating cartilage diseases. In this review, we look at the role of mitochondrial dysfunction in degenerative cartilage diseases and mitochondria as tools in the treatment of osteoarthritis and rheumatoid arthritis.
Short peptides are known to play an important role in modulating transcription, in transmitting b... more Short peptides are known to play an important role in modulating transcription, in transmitting biological information and in restoring the genetic alterations that occur with aging. This paper aims to describe a method of identifying the population of peptides within a peptide cocktail formulation. A sample of lung-derived Mito Organelle (MO) Peptides (LBS) of specific pathogen free (SPF) mammalian rabbits sourced from Charles River Labs were analyzed by mass spectrometry and chromatograms were generated for further examination. The experimentally derived peaks were compared between two batches of LBS MO peptides using MALDI-ToF mass spectroscopy. The following report outlines the experimental methods and the results from performing MALDI-ToF mass spectrometry on various peptides from the company European Wellness (EW).
Stem cell therapy is an attractive treatment for diseases in companion animals that cannot be tre... more Stem cell therapy is an attractive treatment for diseases in companion animals that cannot be treated by conventional veterinary medicine practices. The unique properties of stem cells, particularly the ability to differentiate into specific cell types, makes them a focal point in regenerative medicine treatments. Stem cell transplantation, especially using mesenchymal stem cells, has been proposed as a means to treat a wide range of injuries and ailments, resulting in tissue regeneration or repair. This review aims to summarize the veterinary use of stem cells for treating age-related and joint diseases, which are common conditions in pets. While additional research is necessary and certain limitations exist, the potential of stem cell therapy for companion animals is immense.
None declared Conflict of interest: None declared Background: Bone marrow stem cells have been sh... more None declared Conflict of interest: None declared Background: Bone marrow stem cells have been shown to be a promising therapeutic strategy for autoimmune diseases. This study aimed to assess the safety and efficacy of autologous hematopoietic stem cell (ABMSC) transplantation without immunoablation used to suppress the autoimmune reaction in 6 children with newly diagnosed autoimmune diabetes mellitus. We monitored the levels of islet cell antibodies (ICA), antibodies against islet antigen-related tyrosine phosphatase 2 (IA2), glutamic acid-decarboxylase (GAD) antibodies, and anti-insulin antibodies (AIA). Material/Methods: Between 2018 and 2022, 6 children (age 6-10 years, average 8 years) recently diagnosed with type 1 diabetes mellitus with the presence of ICA, IA2, GAD, AIA and ketoacidosis, were treated with an ABMSC stimulated with Filgrastim, granulocyte colony-stimulating factor (G-CSF), 10 ug/kg/day for 4 days. Bone marrow was harvested on day 5, collected by puncture and identified as mononuclear cells >180×10 6 /kg, CD34+ >0.22%, and transplanted by intravenous (i.v.) infusion. Patients were monitored with ICA, IA2, GAD, AIA, C-peptide, blood glucose, and glycosylated hemoglobin A1c (HbA1C) 6 months after the procedure. Results: At 6-month follow-up, we observed a negative value of the ICA, which was previously positive (P<0.001). The IA2 (p=0.037) and GAD (P=0.377) antibodies decreased slowly but were significantly lower. AIA remained high. A decrease in blood glucose and HbA1C levels was observed (P<0.001). No complications occurred during follow-up. Conclusions: Autologous hematopoietic stem cell transplantation without immunoablation was safe and effective in significantly decreasing the production and effect of autoantibodies against ICA, GAD, and IA2, as well as decreasing blood sugar levels and HbA1c.
Who should represent the public interest? When ordinary citizens, lay members of the public, beco... more Who should represent the public interest? When ordinary citizens, lay members of the public, become patients, who should speak for them? Should doctors and scientists defend patients&amp;amp;amp;amp;#x27; interests? Should a state body represent them? Or should committees be ...
This article is an open access article distributed under the terms and conditions of the Creative... more This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY
BackgroundBone marrow stem cells have been shown to be a promising therapeutic strategy for autoi... more BackgroundBone marrow stem cells have been shown to be a promising therapeutic strategy for autoimmune diseases. This study aimed to assess the safety and efficacy of autologous hematopoietic stem cell (ABMSC) transplantation without immunoablation used to suppress the autoimmune reaction in 6 children with newly diagnosed autoimmune diabetes mellitus. We monitored the levels of islet cell antibodies (ICA), antibodies against islet antigen-related tyrosine phosphatase 2 (IA2), glutamic acid-decarboxylase (GAD) antibodies, and anti-insulin antibodies (AIA).Material/MethodsBetween 2018 and 2022, 6 children (age 6–10 years, average 8 years) recently diagnosed with type 1 diabetes mellitus with the presence of ICA, IA2, GAD, AIA and ketoacidosis, were treated with an ABMSC stimulated with Filgrastim, granulocyte colony-stimulating factor (G-CSF), 10 ug/kg/day for 4 days. Bone marrow was harvested on day 5, collected by puncture and identified as mononuclear cells >180×106/kg, CD34+ >0.22%, and transplanted by intravenous (i.v.) infusion. Patients were monitored with ICA, IA2, GAD, AIA, C-peptide, blood glucose, and glycosylated hemoglobin A1c (HbA1C) 6 months after the procedure.ResultsAt 6-month follow-up, we observed a negative value of the ICA, which was previously positive (P<0.001). The IA2 (p=0.037) and GAD (P=0.377) antibodies decreased slowly but were significantly lower. AIA remained high. A decrease in blood glucose and HbA1C levels was observed (P<0.001). No complications occurred during follow-up.ConclusionsAutologous hematopoietic stem cell transplantation without immunoablation was safe and effective in significantly decreasing the production and effect of autoantibodies against ICA, GAD, and IA2, as well as decreasing blood sugar levels and HbA1c.
International journal of biomedical research & practice, Dec 31, 2022
Mitochondria are major organelles that produce energy for cellular metabolism and are vital to pr... more Mitochondria are major organelles that produce energy for cellular metabolism and are vital to proper cell functioning. Metabolic dysregulation is a critical contributing factor to the initiation and development of degenerative and autoimmune diseases, thus is present in cartilage disorders such as osteoarthritis and rheumatoid arthritis. Mitochondrial dysfunction in cartilage diseases is especially interesting because it may present a promising therapeutic and anti-ageing target. By understanding the mechanism of mitochondrial dysfunction, we can aim to upregulate the role of mitochondria in treating cartilage diseases. In this review, we look at the role of mitochondrial dysfunction in degenerative cartilage diseases and mitochondria as tools in the treatment of osteoarthritis and rheumatoid arthritis.
Short peptides are known to play an important role in modulating transcription, in transmitting b... more Short peptides are known to play an important role in modulating transcription, in transmitting biological information and in restoring the genetic alterations that occur with aging. This paper aims to describe a method of identifying the population of peptides within a peptide cocktail formulation. A sample of lung-derived Mito Organelle (MO) Peptides (LBS) of specific pathogen free (SPF) mammalian rabbits sourced from Charles River Labs were analyzed by mass spectrometry and chromatograms were generated for further examination. The experimentally derived peaks were compared between two batches of LBS MO peptides using MALDI-ToF mass spectroscopy. The following report outlines the experimental methods and the results from performing MALDI-ToF mass spectrometry on various peptides from the company European Wellness (EW).
Stem cell therapy is an attractive treatment for diseases in companion animals that cannot be tre... more Stem cell therapy is an attractive treatment for diseases in companion animals that cannot be treated by conventional veterinary medicine practices. The unique properties of stem cells, particularly the ability to differentiate into specific cell types, makes them a focal point in regenerative medicine treatments. Stem cell transplantation, especially using mesenchymal stem cells, has been proposed as a means to treat a wide range of injuries and ailments, resulting in tissue regeneration or repair. This review aims to summarize the veterinary use of stem cells for treating age-related and joint diseases, which are common conditions in pets. While additional research is necessary and certain limitations exist, the potential of stem cell therapy for companion animals is immense.
None declared Conflict of interest: None declared Background: Bone marrow stem cells have been sh... more None declared Conflict of interest: None declared Background: Bone marrow stem cells have been shown to be a promising therapeutic strategy for autoimmune diseases. This study aimed to assess the safety and efficacy of autologous hematopoietic stem cell (ABMSC) transplantation without immunoablation used to suppress the autoimmune reaction in 6 children with newly diagnosed autoimmune diabetes mellitus. We monitored the levels of islet cell antibodies (ICA), antibodies against islet antigen-related tyrosine phosphatase 2 (IA2), glutamic acid-decarboxylase (GAD) antibodies, and anti-insulin antibodies (AIA). Material/Methods: Between 2018 and 2022, 6 children (age 6-10 years, average 8 years) recently diagnosed with type 1 diabetes mellitus with the presence of ICA, IA2, GAD, AIA and ketoacidosis, were treated with an ABMSC stimulated with Filgrastim, granulocyte colony-stimulating factor (G-CSF), 10 ug/kg/day for 4 days. Bone marrow was harvested on day 5, collected by puncture and identified as mononuclear cells >180×10 6 /kg, CD34+ >0.22%, and transplanted by intravenous (i.v.) infusion. Patients were monitored with ICA, IA2, GAD, AIA, C-peptide, blood glucose, and glycosylated hemoglobin A1c (HbA1C) 6 months after the procedure. Results: At 6-month follow-up, we observed a negative value of the ICA, which was previously positive (P<0.001). The IA2 (p=0.037) and GAD (P=0.377) antibodies decreased slowly but were significantly lower. AIA remained high. A decrease in blood glucose and HbA1C levels was observed (P<0.001). No complications occurred during follow-up. Conclusions: Autologous hematopoietic stem cell transplantation without immunoablation was safe and effective in significantly decreasing the production and effect of autoantibodies against ICA, GAD, and IA2, as well as decreasing blood sugar levels and HbA1c.
Who should represent the public interest? When ordinary citizens, lay members of the public, beco... more Who should represent the public interest? When ordinary citizens, lay members of the public, become patients, who should speak for them? Should doctors and scientists defend patients&amp;amp;amp;amp;#x27; interests? Should a state body represent them? Or should committees be ...
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