Papers by Cleonice Teixeira
Revista Sul-brasiliera de …
ABSTRACT Introduction and objective: The objective of this study was to evaluate ex vivo the read... more ABSTRACT Introduction and objective: The objective of this study was to evaluate ex vivo the reading accuracy of three electronic apex locators – Root ZX, Bingo 1020 and Ipex –, in respect to the real measure of the teeth.Material and methods: Fifty single root premolar teeth extracted were selected. After coronary access, the length tooth (LT) measurement was directly realized by the insertion of a K#10 file in the canal until its tip was observed in the apical foramen with the help of a magnifying glass (8X). After removing the file, its length was recorded with accuracy of 0.01 mm using digital caliper. Then, the teeth were electronically measured (LE) whit the three apex locators until achieving the zero mark on the display of each unit. The electronically measures obtained were compared with the LT and the differences were analyzed by the Kruskal-Wallis Test and by the test of proportions (α = 0.05). Results: It was found that the Root ZX system was statistically different from the Ipex (p < 0.01), and the Bingo system presented intermediary result, showing to be statistically similar to the Root ZX and Ipex systems (p > 0.05). Within the limit of tolerance of ± 1.0 mm, the results demonstrated an accuracy of 100% for ZX Root, 94% for Bingo and 90% for Ipex when compared to the LT values. However, in the limits of ± 0.5 mm the accuracy achieved was 90% for Root ZX, 68% for Bingo and 52% for the Ipex. Conclusion: It was concluded that all electronic devices tested were able in determining the precise tooth length when considered a variation of 1 mm from the position of apical foramen. Nevertheless, when considered a variation of 0.5 mm, only the unit Root ZX proved to be accurate.
Clinical Oral Investigations
RSBO
Introdução e objetivo: O objetivo do presente estudo foi avaliar exvivo a precisão da leitura de ... more Introdução e objetivo: O objetivo do presente estudo foi avaliar exvivo a precisão da leitura de três localizadores apicais eletrônicos –Root ZX, Bingo 1020 e Ipex –, em relação à medida real dos dentes.Material e métodos: Foram selecionados 50 dentes pré-molaresextraídos, unirradiculares. Após a abertura coronária, a mensuração do comprimento do dente (CD) foi realizada diretamente pela inserçãode uma lima K#10 no canal até que sua ponta fosse observada noforame apical com auxílio de uma lente de aumento (8X). Após aremoção da lima, seu comprimento foi registrado com precisão de0,01 mm com uso de paquímetro digital. Em seguida, os dentes forammensurados eletronicamente (CE) com os três localizadores apicaisaté atingir a marca 0 no visor de cada aparelho. As medidas obtidaseletronicamente foram comparadas com o CD, e as diferenças,analisadas pelo teste de Kruskal-Wallis e pelo teste de proporções(α = 0,05). Resultados: Verificou-se que o sistema Root ZX foidiferente do Ipex (p < ...
General dentistry, 2021
The aim of this in vitro study was to evaluate the effect of different periods of intracanal calc... more The aim of this in vitro study was to evaluate the effect of different periods of intracanal calcium hydroxide (Ca[OH]2) dressing application on the compressive strength of root dentin. The roots of 80 human mandibular premolars were prepared via the crown-down technique and randomly distributed into 1 control group (Gc) and 4 experimental groups (n = 16). The experimental groups were based on the length of intracanal Ca(OH)2 dressing use: 7 (G7d), 14 (G14d), 30 (G30d), or 90 (G90d) days. The prepared roots in the control group were left unfilled. The root canals in the experimental groups were dried, filled with the Ca(OH)2 dressing, and radiographed, and then the root canal entrance was sealed. All of the specimens were stored in saline solution at 37°C for the duration of their experimental period. After storage, the roots were sectioned with 2 cuts. The first cut was 1 mm below the cementoenamel junction, and the second was 6 mm apical to the first cut, creating 6-mm-long × 3-mm...
Microscopy Research and Technique, 2020
The purpose of this study was to investigate the tissue reaction stimulated by BaSO4‐ and Bi2O3‐c... more The purpose of this study was to investigate the tissue reaction stimulated by BaSO4‐ and Bi2O3‐containing White MTA Angelus, in comparison with Bi2O3‐containing white Portland cement, and white ProRoot MTA. Thirty‐six adult male Wistar rats (Rattus norvegicus), weighing between 250 and 300 g, were distributed into three groups (n = 12) in accordance with the period of sacrifice (15, 30, and 60 days). Four polyethylene tubes filled with the tested cements were implanted into the dorsum of each rat. Lateral wall of the tubes served as the negative control. After the experimental periods, the animals were euthanized by overdose of pentobarbital anesthetic solution, and the specimens were prepared for microscopic analysis under ×50, ×100, and ×400 magnifications. Inflammatory scores (0–3) were used to grade the tissue reaction. Data were analyzed by the Kruskal‐Wallis test and Dunn's test for individual comparisons (p < .05). A mild to moderate inflammatory tissue reaction was observed at the 15‐day period, which decreased over the course of the periods for all cements, except for Portland cement. There was no significant difference among the tissue responses for ProRoot MTA, BaSO4‐ and Bi2O3‐containing White MTA Angelus at the 60‐day period (p > .05). The Portland group had moderate inflammatory reaction at the final period of analysis, which was statistically different when compared to the other groups (p < .05). The microscopic findings of this animal study suggest that the addition of BaSO4 to White MTA Angelus does not hampers the biocompatibility of the cement.
International Endodontic Journal, 2020
Aim To evaluate the influence of the location and design of endodontic access cavities on root ca... more Aim To evaluate the influence of the location and design of endodontic access cavities on root canal shaping and filling ability, pulp chamber cleaning and fracture resistance of extracted human mandibular incisors. Methodology After pre-selection using periapical radiographs, forty extracted intact human mandibular incisors were scanned in a micro-computed tomographic device. The teeth were matched based on similar anatomical features of the canals and assigned to four experimental groups (n=10) according to the endodontic access cavity and root canal preparation protocol: traditional/TRUShape (T/TRU); traditional/MTwo (T/MT); minimally invasive/TRUShape (MI/TRU); and minimally invasive/MTwo (MI/MT). The samples were scanned after root canal instrumentation and filling procedures. The parameters evaluated were: volume and area of the root canal, non-instrumented canal areas, canal transportation and centering ratio, accumulated hard tissue debris, voids in root canal fillings and root canal filling materials remnants in the pulp chamber. After root canal filling and cavity restoration procedures, the samples were submitted to the fracture resistance test. Data were statistically analysed using Shapiro-Wilk, one-way ANOVA and Bonferroni tests with a significance level of 5% (α=0.05). Results There was no difference regarding all parameters evaluated before and after root canal preparation (volume and area of the root canal, non-instrumented canal areas, canal transportation and centering ratio, and accumulated hard tissue debris) among the groups (P>0.05). MI/TRU and MI/MT groups were associated with significantly more voids in root canal fillings when compared to the T/TRU and T/MT groups (P<0.05). Percentage of root canal filling material remnants in the pulp chamber after cleaning procedures and mean fracture resistance values were not ssignificantly different among the four experimental groups (P>0.05). Conclusions The location and design of the endodontic access cavity did not impact on the root canal preparation and resistance to fracture of extracted mandibular incisors, regardless of the instrument used. Minimally invasive access cavities were associated with significantly more voids in root canal fillings.
Microscopy Research and Technique, 2020
Introduction: The aim of this study was to evaluate the effect of calcium hydroxide (CH) and anti... more Introduction: The aim of this study was to evaluate the effect of calcium hydroxide (CH) and antibiotic pastes, including a mixture of metronidazole and ciprofloxacin, with and without minocycline or cefaclor, on the dislocation resistance of mineral trioxide aggregate (MTA) to root dentin. Methods: Eighty single-rooted human mandibular premolars were selected. The teeth were prepared by using the ProTaper system. The prepared teeth were then instrumented to a #6 Peeso reamer to obtain a standard internal diameter of 1.5 mm. The reamers were passed 1 mm beyond apex to simulate immature teeth. The specimens were then randomly divided into a control group (no intracanal medicament was used) and 4 experimental groups that were treated with an intracanal medicament: CH, double antibiotic paste (DAP) with metronidazole and ciprofloxacin, triple antibiotic paste (TAP) with minocycline, or TAP with cefaclor (n = 16). After 3 weeks, the medicaments were removed, and approximately 3 mm of MTA was placed in the coronal third of the canals. A push-out test was used to measure the dislocation resistance between the root dentin and MTA. Data were analyzed by using one-way analysis of variance and Tukey post hoc tests. Results: The dislocation resistance values of the CH, TAP with minocycline, and TAP with cefaclor groups were similar to those of the control group (P > .05), whereas the DAP group had the lowest dislocation resistance when compared with the other groups (P < .05). Overall, there was a predominance of cohesive failures between root dentin and MTA. Conclusions: The results of this study indicate that the application of DAP as an intracanal medicament reduced the dislocation resistance of MTA to root dentin.
Clinical Oral Investigations, 2020
Objective Traumatic dental injuries often affect the dental hard tissues, periodontal tissues, an... more Objective Traumatic dental injuries often affect the dental hard tissues, periodontal tissues, and dental pulp. Root resorption (RR) is a significantly concerning phenomenon that could lead to loss of the traumatized tooth. The purpose of this systematic review was to analyze the incidences of different types of RR after concussion, subluxation, lateral luxation, intrusive luxation, and extrusive luxation. Material and methods Seven databases were electronically and manually searched for the identification of observational studies that evaluated the incidence of RR after luxation injuries. Following study selection, data extraction, and risk of bias assessment through MAStARI checklist, the GRADE quality of available evidence was assessed. Results In total, 14 studies were included in the present systematic review. Ten and four studies presented a moderate and low RoB, respectively, and the overall GRADE quality of evidence was "very low" for all outcomes. The highest incidence rates of RR were observed for teeth with intrusive luxation, followed by those with extrusive luxation, lateral luxation, subluxation, and concussion. In general, the most common type of RR documented for all injuries was inflammatory RR, followed by replacement RR, surface RR, and internal RR. Conclusions Our findings suggest that RR most commonly occurs in association with intrusive luxation and by inflammatory RR. Awareness regarding the incidence of RR after injuries is useful for clinicians to minimize the risk and severity of its occurrence, because a late diagnosis of RR may limit treatment alternatives and result in tooth loss. Clinical relevance Knowledge about the incidence of RR in teeth with concussion and different types of luxation injuries can ensure appropriate follow-up protocols and favorable outcomes.
Brazilian Dental Journal, 2019
This study correlated the bond strength (BS) and dentin penetration of different sealers by push-... more This study correlated the bond strength (BS) and dentin penetration of different sealers by push-out test and Confocal Laser Scanning Microscopy (CLSM) analysis. Forty-five root canals were prepared according to the crown-down technique and filled with gutta-percha associated to the following sealers (n=15): Endofill, AH Plus and MTA Fillapex. Five canals of each group were filled with the sealers added with 0.1% Rhodamine B dye. Next, the specimens were transversely sectioned and submitted to the push-out test (n=10) and CLSM analysis (n=5). The BS data showed the following means (MPa) and standard deviation: AH Plus (4.17±1.86); MTA Fillapex (3.13±1.96) and Endofill (2.10±1.03). Statistical analysis (two-way ANOVA, α=0.05) showed significant difference among sealers (p<0.001) and root canal thirds (p<0.001). The BS results of Endofill and MTA Fillapex were statistically similar (p>0.05), however, they were statistically different from AH Plus (p<0.001). The regional an...
European Journal of Dentistry, 2019
Objective The purpose of this study was to evaluate the effect of the intracoronary bleaching and... more Objective The purpose of this study was to evaluate the effect of the intracoronary bleaching and calcium hydroxide (Ca(OH)2) dressing use, on the bond strength (BS) of fiberglass posts to root dentine. Materials and Methods After root canal filling of 40 bovine incisors, a 2-mm thick cervical plug was fabricated 2 mm below the cementum–enamel junction. Seven days later, teeth were randomly distributed into four groups (n = 10), as follows: G1 no bleaching, followed by immediate post cementation; G2 bleaching and immediate post cementation; G3 bleaching, dressing with Ca(OH)2 for 7 days, and post cementation; and G4 bleaching, no dressing, and post cementation after 7 days. The roots were transversally cut into 1-mm thick slices to perform the push-out test (0.5 mm/min). Failure modes were assessed under scanning electron microscopy. Statistical Analysis The analysis of variance (two-way ANOVA) was performed, followed by the supplementary Tukey multiple comparison test (a = 5%). Res...
Clinical oral investigations, Jan 17, 2018
To compare the accuracy of confocal laser scanning microscopy (CLSM) and scanning electron micros... more To compare the accuracy of confocal laser scanning microscopy (CLSM) and scanning electron microscopy (SEM) during the analysis of the adhesive interface integrity and intratubular penetration of root canal sealers to radicular dentine. Twenty roots of human maxillary incisors were prepared and distributed into two groups (n = 10), followed by filling with gutta-percha and Endofill (G1) or AH Plus (G2). After 7 days, roots were sectioned and analyzed under CLSM and SEM. Score systems were used to evaluate the adhesive interface integrity (0-4) and sealer intratubular penetration (0-3). Data were submitted to Wilcoxon-Mann-Whitney and Kendall correlation statistical tests (α = 5%). In the adhesive interface analysis, CLSM was similar (P = 0.157) to SEM for Endofill; however, the results were different for AH Plus (P = 0.029). Intratubular penetration had significant difference between observational methods for both sealers (P < 0.0001). Correlation analysis between SEM and CLSM fo...
Clinical oral investigations, Jan 3, 2017
This systematic review was performed to answer the following question: do contracted endodontic c... more This systematic review was performed to answer the following question: do contracted endodontic cavities (CECs) increase resistance to fracture in extracted human teeth compared to traditional endodontic cavities (TECs)? A literature search without restrictions was carried out in PubMed, Science Direct, Scopus, Web of Science, and Open Grey databases. Articles were selected by two independent reviewers. In addition, a reference and hand search was also fulfilled. All included in vitro studies evaluated the influence of CECs on strength to fracture in extracted human teeth and compared to TECs. The quality of the selected studies was evaluated and they were classified as having a low, moderate or high risk of bias. A total of 810 articles were obtained in the electronic search. After the application of the eligibility criteria, reference and hand search, and duplicate removal, six studies were included in this systematic review. All included studies evaluated the influence of CECs on...
Journal of endodontics, Jan 21, 2017
The aim of this study was to assess the influence of contracted endodontic cavities (CECs) on roo... more The aim of this study was to assess the influence of contracted endodontic cavities (CECs) on root canal detection, instrumentation efficacy, and fracture resistance assessed in maxillary molars. Traditional endodontic cavities (TECs) were used as a reference for comparison. Thirty extracted intact maxillary first molars were scanned with micro-computed tomographic imaging at a resolution of 21 μm, assigned to the CEC or TEC group (n = 15/group), and accessed accordingly. Root canal detection was performed in 3 stages: (1) no magnification, (2) under an operating microscope (OM), and (3) under an OM and ultrasonic troughing. After root canal preparation with Reciproc instruments (VDW GmbH, Munich, Germany), the specimens were scanned again. The noninstrumented canal area, hard tissue debris accumulation, canal transportation, and centering ratio were analyzed. After root canal filling and cavity restoration, the sample was submitted to the fracture resistance test. Data were analyze...
Objectives: This study verified the Vickers hardness (VHN) of a composite resin (CR) after root r... more Objectives: This study verified the Vickers hardness (VHN) of a composite resin (CR) after root reinforcement, according to the light-curing time, root region and storage period. Methods: Twenty 17-mm long roots were used. Twenty-four hours after obturation, the canal was emptied to a depth of 12 mm, enlarged with diamond points, filled with CR and light-activated through the DT Light fiber post for either 40 s or 120 s. After 24 h, each root was sectioned into 3 slices at depths of 2, 6 and 10 mm (n = 30). The initial VHN of the CR was measured as the average of 3 indentations (100g/15s), at lateral distances of 50, 200 and 350 μm from the cement/post. The specimens were stored in water for 18 months and the final VH was measured. Results: The ANOVA for repeated measures (α = 0.05) indicated that the factors light-curing time, reinforced region and storage period influenced VH values. In the deepest regions, the hardness of CR was lower, regardless of the storage period. The mean V...
Objective: To investigate the effectiveness of various storage media in maintaining the viability... more Objective: To investigate the effectiveness of various storage media in maintaining the viability of human periodontal ligament fibroblasts (PDLF) at 20C. Methods: Plates with PDLF were soaked in natural coconut water, industrialized coconut water, skimmed milk, whole milk, recently prepared Hank's balanced salt solution (HBSS), Save-A-Tooth system's HBSS (Save), and tap water at 20C for 3, 6, 24, 48, 72, 96 and 120 h. Minimum essential medium at 37C (MEM-37) was used as positive control. After incubation, viability of the cells was determined using the tetrazolium salt-based colorimetric (MTT) assay. Statistical analysis of the data was accomplished by using Kruskal-Wallis test complemented by Scheff test (α=5%). Results: From 24 h onwards, coconut water was significantly better than all other media. Save and industrialized coconut water were the worst storage media. Skimmed milk and whole milk were significantly better than HBSS for up 72 h. Conclusion: The effectiveness ...
Objective: To evaluate the influence of apical patency on the periapical healing process in pulpl... more Objective: To evaluate the influence of apical patency on the periapical healing process in pulpless dogs’ teeth after root canal filling with AH Plus sealer. Methods: Seventy-eight root canals of dogs’ teeth were used. After pulp extirpation, the cemental canal was penetrated and widened up to a size 20 K-file. After, the canals were exposed to the oral cavity for 3 months for contamination. Root canals were randomly divided into 6 groups (n=13), and prepared up to size 50 K-file, maintaining (PAT) or not (NPAT) the apical patency with a size15 K-file, and dressing or not the root canals with calcium hydroxide paste (CH) for 30 or 7 days, as described: Group 1: PAT+CH for 30 days; Group 2: NPAT+CH for 30 days; Group 3: PAT+CH for 7 days; Group 4: NPAT+CH for 7 days; Group 5: PAT+ immediate root canal filling; and Group 6: NPAT+ immediate root canal filling. After 6 months, the jaws were prepared for histological analysis, and sections evaluated for quality of the apical foramen clo...
Objective: The aim of this study was to analyze the interaction of the new cements MTA Fillapex, ... more Objective: The aim of this study was to analyze the interaction of the new cements MTA Fillapex, Diaroot Bioaggregate and iRoot SP with dentin in vivo. Method: One hundred and sixty human dentin tubes were randomly divided in 4 experimental groups. After filling with the materials tubes were implanted subcutaneously in 32 rats at four sites equidistant from the dorsal area. Empty tubes were used as control and implanted in a fifth site. After 7, 15, 30 and 90 days the animals were euthanized and the dentin tubes retrieved for scanning electron microscope (SEM) analysis. Result: In 7 and 30 days, the mineral deposition in the material-dentin interface was detected in all tubes filled with MTA Fillapex; and in 15 and 90 days it was detected in 7 of the 8 samples. Despite of the period, the mineral deposition was not detected in any of the control samples, neither in those filled with iRoot SP, Diaroot Bioaggregate and MTA Branco. Conclusion: It was concluded that MTA FILLAPEX was the ...
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Papers by Cleonice Teixeira