Papers by Chandan kumar Debnath
Effect of �-glucan on immunity and survival of early stage of Anabas testudineus (Bloch)
Fish Shellfish Immunol, 2009
69 Effect of β-glucan on the immune response of
SpringerPlus, 2013
The present study was carried out to study the effect of different dosages of β-glucan suspension... more The present study was carried out to study the effect of different dosages of β-glucan suspension on immune response and disease resistance in Anabas testudineus spawns against a fungal pathogen Saprolegnia parasitica. Eight day old spawns were exposed for 3 h in four different dosages of β-glucan suspension in phosphate buffered saline at the rate of 0, 5, 10, 15 mg l-1. The cell suspension of spawn was assayed for total protein, acid phosphate, lysozyme, bactericidal and NBT activity. The spawns were then challenged with 3x10 5 CFU ml-1 of S. parasitica. The survivability percentage and immunological parameters were assayed upto day 7. Exposure of fish for 3h to all the concentrations of β-glucan recorded a significant enhancement in the immunological parameters such as lysozyme activity, bactericidal activity and NBT activity by the end of day 7. The challenge study indicated least mortality in the groups exposed to 10 mgl-1 and 15 mgl-1 but 15 mgl-1 gives little higher survivability. Thus 3 h exposure of β-glucan suspension could reduce the mortality and increase the immunity of A. testudineus spawns against S. parasitica.
Effect of β-glucan on immunity and survival of early stage of Anabas testudineus (Bloch)
Fish & Shellfish Immunology, 2009
The present experiment was carried out to study the effect of different dosages of beta-glucan su... more The present experiment was carried out to study the effect of different dosages of beta-glucan suspension derived from barley on the innate immune response and disease resistance of Anabas testudineus spawns against infection caused by Aeromonas hydrophila. Four different dosages of beta-glucan suspension in phosphate buffered saline at the rate of 0, 5, 10, 15 mg l(-1) were taken and 8 days old spawn were exposed for 2 h and 3 h. The cell suspension of spawn was assayed for total protein, acid phosphatase activity, lysozyme activity, bactericidal and NBT. Further, the spawns were challenged with 3 x 10(5) cells ml(-1) of A. hydrophila and survivability percentage and immunological parameters were assayed upto day 7. On day 7, most of the immunological parameters such as lysozyme activity, bactericidal activity and NBT activity were significantly enhanced after exposing the fish to all the concentrations of beta-glucan. Challenge study indicated least mortality in the group of spawns immersed in 15 mg l(-1) beta-glucan suspension for 3 h. Thus, 3 h exposure to beta-glucan suspension could reduce the mortality and increase the immunity of A. testudineus spawns.
Uploads
Papers by Chandan kumar Debnath