The results about extractive distillation of water-ethanol mixtures with PAW polyalcohol containi... more The results about extractive distillation of water-ethanol mixtures with PAW polyalcohol containing CaCl2 and KCOOCH3 salts disolved in the polyalcohol, using a unitary volumetric relation water- ethanol mixture to polyalcohol and a concentration of 0.1 g/mL of each salt in the polyalcohol are presented. It was found that the polyalcohol PAW modified the water-ethanol azeotropic behavior as a function of
SPE Annual Technical Conference and Exhibition, 2011
Abstract Since the inclusion of Micro Seismic technologies in the Barnett Shale, this technology ... more Abstract Since the inclusion of Micro Seismic technologies in the Barnett Shale, this technology has become one of the most powerful and used tools to monitor the behavior of reservoirs exposed to hydraulic fracturing with non-permanent arrays and also, to detect ...
Electron microscope images of negative-stained particles of FMDV have been analyzed. The viral ca... more Electron microscope images of negative-stained particles of FMDV have been analyzed. The viral capsid appeared to be a pliant structure composed of monomers having an average width of about 2.5 nm and a length of 4 nm. Upon capsid disruption, a sequential and ...
... Biophys. Acta. 262, 220226 (1972). 16. VASQUEZ, C., PALMA, EL, and BARRANTES, F. J,, J. Micro... more ... Biophys. Acta. 262, 220226 (1972). 16. VASQUEZ, C., PALMA, EL, and BARRANTES, F. J,, J. Microsc. 14, 147154 (1972). 17. ... 15111518. Full Text via CrossRef | View Record in Scopus | Cited By in Scopus (19). 24. JJ Pene, E. Knight, Jr and JE Darnell, Jr. J. Mol. Biol. ...
A processing endoribonuclease was isolated from the cytoplasm of chick embryos. The enzyme was ea... more A processing endoribonuclease was isolated from the cytoplasm of chick embryos. The enzyme was easily obtained using an RNA extraction procedure based on a mild deproteinization with Sarkosyl and cold phenol/ chloroform. This technique assured the recovery of several proteins and the endoribonuclease in association with the RNA.
The effect on phage morphogenesis of sus mutations in the cistrons coding for nonstructural prote... more The effect on phage morphogenesis of sus mutations in the cistrons coding for nonstructural proteins has been studied. Mutants in three cistrons analyzed that are involved in phage DNA synthesis, as well as in cistron 16 which codes for a late nonstructural protein, produce prolate capsids which are more rounded at the corners than complete phage heads and have an internal core; they contain the head proteins, the upper collar protein and protein p7, not present in mature phage particles. Mutants in cistron 7 do not produce capsids nor other phage-related structures; this result and the presence of p7 in phage capsids suggest an essential role in capsid assembly for this protein. The protein product of cistron 13 is probably needed for a stable DNA encapsulation since mutants in this cistron produce mainly DNA-free complete phage particles and only about 10 % of uninfective DNA-containing complete phage. Cistron 15 codes for a late, partially dispensable, nonstructural protein which is present in the DNA-free capsids produced after infection with the delayed-lysis mutant sus14(1242), used as the wild-type control, or with mutants in cistrons 9, 11, 12 and 13. Proteins pl5 and p16 are probably involved in the encapsulation of viral DNA in a prohead.
THE DNA of the B. subtilis phage ϕ29 has been described as unpermuted linear duplex molecules1 of... more THE DNA of the B. subtilis phage ϕ29 has been described as unpermuted linear duplex molecules1 of molecular weight 11 × 106, but the formation of circular molecules has also been indicated, suggesting the existence of cohesive ends1,2.
Twisted DNA rings were released from polyoma virus particles upon spreading on a protein film ove... more Twisted DNA rings were released from polyoma virus particles upon spreading on a protein film over a denaturing hypophase of urea and sodium perchlorate. Conformational changes were observed while the DNA molecules were irreversibly adsorbed to the monolayer. In our preparations, 80% of the DNA rings fist appeared as twisted molecules and 15% as open rings, before all molecules in the monolayer were slowly denatured to tightly coiled forms. 5% were linear forms.
Electron micrographs have been obtained of proteolipid macromolecules from cerebral cortex during... more Electron micrographs have been obtained of proteolipid macromolecules from cerebral cortex during the successive steps of extraction and purification. These macromolecules were seen as short filaments having a width of about 15 A and a minimum length of 150 li and showed a high tendency to aggregate. This study was mainly concentrated on the "receptor proteolipid" which, by previous studies, was shown to have high affinity binding for different drugs active in nerve transmission. The binding of atropine sulphate to the proteolipid macromolecules was followed and different patterns of organization were observed. Ordered arrays of fibres, forming tubule-like structures were found with low concentrations of the drug. Then, as the concentration of atropine sulphate increased a drastic change occurred consisting of aggregation and the formation of amorphous clusters of proteolipid macromolecules. 221 222 C. VASQUEZ ET AL.
A special technique for the electron-microscope study of the fine structure of ultrathin artifici... more A special technique for the electron-microscope study of the fine structure of ultrathin artificial membranes is described. Membranes made of total phospholipids of the cerebral cortex and cholesterol showed globular elements of 40 ~ embedded in a denser and diffuse matrix. These same elements were also seen organized in a periodic banded pattern. Identical patterns were observed with and without supporting films. Lipidic membranes containing small amounts of proteolipid from Electrophorus showed a lower electron density, a finer and smoother texture and a decrease in electrical resistance.
CON FECHA 30 DE NOVIEMBRE DEL 2011 NUEVE REGIDORES DEL CONSEJO PROVINCIAL DE SAN MARTÍN A SOLICIT... more CON FECHA 30 DE NOVIEMBRE DEL 2011 NUEVE REGIDORES DEL CONSEJO PROVINCIAL DE SAN MARTÍN A SOLICITUD DE LA REGIDORA KAROL IVETT PAREDES FONSECA, CESARON EN FORMA ARBITRIA E ILEGAL AL GERENTE MUNICIPAL LICENCIADO RICADO ZELAYA MORENO, SIN NINGÚN FUNDAMENTO, SIN JUSTIFICACIÓN ALGUNA, SIN DEBIDO PROCESO, DEMOSTRANDO PREPOTENCIA, SOBERBIA E IGNORANCIA. ESTE HECHO MERECIÓ LA SANCIÓN EJEMPLAR DE VACANCIA APLICADA POR EL JURADO NACIONAL DE ELECCIONES, ANTE LA DENUNCIA DE LOS CIUDADANOS JARRY MOZOMBITE PINCHI, ANTONIO BELDA NECIOSUP Y HOMERO VALLES GÁRATE, CUYO TEXTO MUY ILUSTRATIVO SE ENCUENTRA EN LA RESOLUCIÓN N° 612-2012-JNE DE FECHA 21 DE JUNIO DEL MISMO AÑO.
The results about extractive distillation of water-ethanol mixtures with PAW polyalcohol containi... more The results about extractive distillation of water-ethanol mixtures with PAW polyalcohol containing CaCl2 and KCOOCH3 salts disolved in the polyalcohol, using a unitary volumetric relation water- ethanol mixture to polyalcohol and a concentration of 0.1 g/mL of each salt in the polyalcohol are presented. It was found that the polyalcohol PAW modified the water-ethanol azeotropic behavior as a function of
SPE Annual Technical Conference and Exhibition, 2011
Abstract Since the inclusion of Micro Seismic technologies in the Barnett Shale, this technology ... more Abstract Since the inclusion of Micro Seismic technologies in the Barnett Shale, this technology has become one of the most powerful and used tools to monitor the behavior of reservoirs exposed to hydraulic fracturing with non-permanent arrays and also, to detect ...
Electron microscope images of negative-stained particles of FMDV have been analyzed. The viral ca... more Electron microscope images of negative-stained particles of FMDV have been analyzed. The viral capsid appeared to be a pliant structure composed of monomers having an average width of about 2.5 nm and a length of 4 nm. Upon capsid disruption, a sequential and ...
... Biophys. Acta. 262, 220226 (1972). 16. VASQUEZ, C., PALMA, EL, and BARRANTES, F. J,, J. Micro... more ... Biophys. Acta. 262, 220226 (1972). 16. VASQUEZ, C., PALMA, EL, and BARRANTES, F. J,, J. Microsc. 14, 147154 (1972). 17. ... 15111518. Full Text via CrossRef | View Record in Scopus | Cited By in Scopus (19). 24. JJ Pene, E. Knight, Jr and JE Darnell, Jr. J. Mol. Biol. ...
A processing endoribonuclease was isolated from the cytoplasm of chick embryos. The enzyme was ea... more A processing endoribonuclease was isolated from the cytoplasm of chick embryos. The enzyme was easily obtained using an RNA extraction procedure based on a mild deproteinization with Sarkosyl and cold phenol/ chloroform. This technique assured the recovery of several proteins and the endoribonuclease in association with the RNA.
The effect on phage morphogenesis of sus mutations in the cistrons coding for nonstructural prote... more The effect on phage morphogenesis of sus mutations in the cistrons coding for nonstructural proteins has been studied. Mutants in three cistrons analyzed that are involved in phage DNA synthesis, as well as in cistron 16 which codes for a late nonstructural protein, produce prolate capsids which are more rounded at the corners than complete phage heads and have an internal core; they contain the head proteins, the upper collar protein and protein p7, not present in mature phage particles. Mutants in cistron 7 do not produce capsids nor other phage-related structures; this result and the presence of p7 in phage capsids suggest an essential role in capsid assembly for this protein. The protein product of cistron 13 is probably needed for a stable DNA encapsulation since mutants in this cistron produce mainly DNA-free complete phage particles and only about 10 % of uninfective DNA-containing complete phage. Cistron 15 codes for a late, partially dispensable, nonstructural protein which is present in the DNA-free capsids produced after infection with the delayed-lysis mutant sus14(1242), used as the wild-type control, or with mutants in cistrons 9, 11, 12 and 13. Proteins pl5 and p16 are probably involved in the encapsulation of viral DNA in a prohead.
THE DNA of the B. subtilis phage ϕ29 has been described as unpermuted linear duplex molecules1 of... more THE DNA of the B. subtilis phage ϕ29 has been described as unpermuted linear duplex molecules1 of molecular weight 11 × 106, but the formation of circular molecules has also been indicated, suggesting the existence of cohesive ends1,2.
Twisted DNA rings were released from polyoma virus particles upon spreading on a protein film ove... more Twisted DNA rings were released from polyoma virus particles upon spreading on a protein film over a denaturing hypophase of urea and sodium perchlorate. Conformational changes were observed while the DNA molecules were irreversibly adsorbed to the monolayer. In our preparations, 80% of the DNA rings fist appeared as twisted molecules and 15% as open rings, before all molecules in the monolayer were slowly denatured to tightly coiled forms. 5% were linear forms.
Electron micrographs have been obtained of proteolipid macromolecules from cerebral cortex during... more Electron micrographs have been obtained of proteolipid macromolecules from cerebral cortex during the successive steps of extraction and purification. These macromolecules were seen as short filaments having a width of about 15 A and a minimum length of 150 li and showed a high tendency to aggregate. This study was mainly concentrated on the "receptor proteolipid" which, by previous studies, was shown to have high affinity binding for different drugs active in nerve transmission. The binding of atropine sulphate to the proteolipid macromolecules was followed and different patterns of organization were observed. Ordered arrays of fibres, forming tubule-like structures were found with low concentrations of the drug. Then, as the concentration of atropine sulphate increased a drastic change occurred consisting of aggregation and the formation of amorphous clusters of proteolipid macromolecules. 221 222 C. VASQUEZ ET AL.
A special technique for the electron-microscope study of the fine structure of ultrathin artifici... more A special technique for the electron-microscope study of the fine structure of ultrathin artificial membranes is described. Membranes made of total phospholipids of the cerebral cortex and cholesterol showed globular elements of 40 ~ embedded in a denser and diffuse matrix. These same elements were also seen organized in a periodic banded pattern. Identical patterns were observed with and without supporting films. Lipidic membranes containing small amounts of proteolipid from Electrophorus showed a lower electron density, a finer and smoother texture and a decrease in electrical resistance.
CON FECHA 30 DE NOVIEMBRE DEL 2011 NUEVE REGIDORES DEL CONSEJO PROVINCIAL DE SAN MARTÍN A SOLICIT... more CON FECHA 30 DE NOVIEMBRE DEL 2011 NUEVE REGIDORES DEL CONSEJO PROVINCIAL DE SAN MARTÍN A SOLICITUD DE LA REGIDORA KAROL IVETT PAREDES FONSECA, CESARON EN FORMA ARBITRIA E ILEGAL AL GERENTE MUNICIPAL LICENCIADO RICADO ZELAYA MORENO, SIN NINGÚN FUNDAMENTO, SIN JUSTIFICACIÓN ALGUNA, SIN DEBIDO PROCESO, DEMOSTRANDO PREPOTENCIA, SOBERBIA E IGNORANCIA. ESTE HECHO MERECIÓ LA SANCIÓN EJEMPLAR DE VACANCIA APLICADA POR EL JURADO NACIONAL DE ELECCIONES, ANTE LA DENUNCIA DE LOS CIUDADANOS JARRY MOZOMBITE PINCHI, ANTONIO BELDA NECIOSUP Y HOMERO VALLES GÁRATE, CUYO TEXTO MUY ILUSTRATIVO SE ENCUENTRA EN LA RESOLUCIÓN N° 612-2012-JNE DE FECHA 21 DE JUNIO DEL MISMO AÑO.
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ESTE HECHO MERECIÓ LA SANCIÓN EJEMPLAR DE VACANCIA APLICADA POR EL JURADO NACIONAL DE ELECCIONES, ANTE LA DENUNCIA DE LOS CIUDADANOS JARRY MOZOMBITE PINCHI, ANTONIO BELDA NECIOSUP Y HOMERO VALLES GÁRATE, CUYO TEXTO MUY ILUSTRATIVO SE ENCUENTRA EN LA RESOLUCIÓN N° 612-2012-JNE DE FECHA 21 DE JUNIO DEL MISMO AÑO.
ESTE HECHO MERECIÓ LA SANCIÓN EJEMPLAR DE VACANCIA APLICADA POR EL JURADO NACIONAL DE ELECCIONES, ANTE LA DENUNCIA DE LOS CIUDADANOS JARRY MOZOMBITE PINCHI, ANTONIO BELDA NECIOSUP Y HOMERO VALLES GÁRATE, CUYO TEXTO MUY ILUSTRATIVO SE ENCUENTRA EN LA RESOLUCIÓN N° 612-2012-JNE DE FECHA 21 DE JUNIO DEL MISMO AÑO.