Massimo Bocchi

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Carleton University

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University of Reading

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Kansas State University

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UNL - New University of Lisbon

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Papers by Massimo Bocchi

Controlled Isolation and Patterning of K562 Leukemia Cells Using Electrically Activated Microchannels

Royal Chemical Society, 2011

We present here an innovative architecture based on negative-dielectrophoresis (nDEP) to perform ... more We present here an innovative architecture based on negative-dielectrophoresis (nDEP) to perform the controlled isolation and patterning of a programmable number of cells inside microwells. The high efficiency solution we propose features electrodes placed on the floor and sidewalls of the microfluidic channels where microwells are realized and allows for the successful isolation, patterning and live-cell imaging of both single cells and cell clusters, with single cell resolution. Cell viability was assessed after delivery performing a calcein release assay in the microwells

Precise Positioning of Particles in Microwells within Horizontal DEP Cages

An efficient method to trap and analyze particles avoiding excessive electric field strength is t... more An efficient method to trap and analyze particles avoiding excessive electric field strength is to use negative dielectrophoretic cages, where particles find stability in the electric field minimum. We present a novel structure where particles are trapped and forced to interact within microwells opened at top and bottom sides by creating a cylindrical negative dielectrophoretic cage. This approach provides a controlled alignment of the particles along a predefined axis. An array of microwells is built by drilling through holes with a diameter of 150 µm on a flexible printed circuit board with two Cu/Au layers separated by one polyimide layer, providing a cheap and disposable device. Levitation of one or two polystyrene beads with a diameter of 25 µm in DI-water was experimented confirming the positioning predicted by physical simulations. Vertical position of the particles as a function of the amplitude of polarization signals was extracted from simulations and confirmed by experime...

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A reconfigurable heterogeneous multiprocessor System on Chip

Reconfigurable IO Module

Abstract 3613: Lab-on-a-chip-based in-vitro functional profiling proves to be effective in predicting therapy outcome in AML patients

Cancer Research, 2018

Functional profiling is an emerging trend in precision medicine. Manual laboratory methods proved... more Functional profiling is an emerging trend in precision medicine. Manual laboratory methods proved to be effective in predicting the response to drug treatments both in patients stratified according to genetic profiling and in patients without any specific molecular aberration. In this work, we validated the predictive power of an innovative functional profiling platform, based on lab-on-a-chip technology, which allows testing the response of live tumor cells exposed to anticancer drugs in a fully-automated process, requiring only a Ficoll stratification as a manual step. 15 AML patients (Age: 40% <60 years, 46% in the 60-70 years range, 14% >70 years; Gender: 40% Female; ELN Classification: 20% Favorable, 26% Intermediate-I, 33% Intermediate-II, 14% Adverse; AML stage: 53% De novo, 7% Relapse, 40% Refractory; Treatment: 20% FLAI-3, 40% FLAI-5, 26% Decitabine; 7% Cytarabine, 7% 5-azacitidine) were treated at the Policlinico Sant'Orsola in Bologna. At the same time, bone mar...

Inter-Cell Networking Profiling Enables Comprehensive Characterization of Immune-Mediated Activity of Anti-CD38 Therapy through Ex-Vivo Analysis of Multiple Myeloma Patients

Blood, 2019

There has been a fast progress in clinical use of antibody-based immunotherapy, given the superio... more There has been a fast progress in clinical use of antibody-based immunotherapy, given the superior efficacy commonly achieved in clinic and the limited toxicity. However, personalization of treatment remains of major importance, both to achieve better clinical performance for monotherapy and to identify the best combinations on a patient-by-patient basis. Predicting patient's response is complex due to the need to characterize both tumor response and immunologic mechanisms possibly activated by the therapy, including antibody dependent cellular cytotoxicity (ADCC). We present the Inter-Cell Networking Profiling (ICNP), a novel analytical method enabling a comprehensive and precise characterization of the modulatory effect of immunotherapies on immune-tumor cell interactions. ICNP works on the Open Microwell (OMW) microfluidic system which recreates 20,000 unique cell clusters from ex-vivo patient samples and exposes them to anticancer drugs. We validated the ICNP using multiple ...

Ex-Vivo Drug Response Profiling for Precision Medicine Approaches in Acute Myeloid Leukemia with the Open Microwell Microfluidic Platform

Blood, 2016

Background: Patient stratification to match individual patients with the most effective drug trea... more Background: Patient stratification to match individual patients with the most effective drug treatment is still a major open challenge in cancer care. For instance, cytarabine is the main drug used for AML treatment but 30% of patients fail to respond to this agent. Laboratory developed tests determining ex-vivo cellular response to cytotoxic anticancer drugs have demonstrated good correlations with clinical response, sometimes surpassing the predictive power of molecular and genetic profiling. Standardizing sample processing to remove operator-dependent biases and maintaining live cells in a functional status that closely resembles in-vivo function are major challenges affecting these tests. Here we present the open microwell (OMW) platform, a microfluidic-based system that integrates the entire process of ex-vivo testing of anticancer drug efficacy and enables drug testing in the clinical setting prior to therapy administration. The concept was validated for the first time on 13 A...

Method and Apparatus for Manipulating Single Cells and Small Aggregates Thereof

A case-study on multimedia applications for the XiRisc reconfigurable processor

A powerful factor in the recent embedded systems market is undoubtedly the strong need to add por... more

A XiRisc-based SoC for embedded DSP applications

Proceedings of the IEEE 2004 Custom Integrated Circuits Conference (IEEE Cat. No.04CH37571)

A system level IP integration methodology for fast SOC design

Proceedings. 2003 International Symposium on System-on-Chip (IEEE Cat. No.03EX748)

In the system-on-chip (SOC) era, the growing number of functionalities included on a single chip ... more In the system-on-chip (SOC) era, the growing number of functionalities included on a single chip requires the development of new design methodologies to keep the design complexity under control. Intellectual property reuse has been commonly employed as a technique to address this problem, but a new system-level approach is needed to integrate IP-Reuse methodology in the designpow, in order to speed up the designer's productivity In this papec a SOC design plarform is proposed as a solution to this problem, pmviding a library ofIP reusable blocks and a high level tool for SOC design development. An IP library based on AMBA bus architecture was built, featuring a collection of devices with homogeneous interfaces described with VHDL language constructs that enable hardware configurabiliiy A system-level assembler (SL4) was then developed to provide a hardware con$guration tool and a suite of utilities to support the designer work. Once defrned the system structure, the SL4 allows automatic generation of the environments used for software development, simulation. synthesis and verification tasks.

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A Multi-Context Pipelined Array for Embedded Systems

2006 International Conference on Field Programmable Logic and Applications, 2006

ARCES (DEIS) University of Bologna, Bologna, Italy email: {andrea.lodi,cmucci}@deis.unibo. it {ac... more

Design and implementation of a reconfigurable heterogeneous multiprocessor SoC

IEEE Custom Integrated Circuits Conference 2006, 2006

This paper introduces a novel heterogeneous shared memory multiprocessor architecture based on a ... more This paper introduces a novel heterogeneous shared memory multiprocessor architecture based on a reconfigurable processor and a standard RISC processor. The work aims at increasing further the computational density of a reconfigurable device by the integration of the reconfigurable hardware into a heterogeneous multi-core architecture. Though a RISC processor has a lower inner computational density than a reconfigurable processor, this

A Cycle-Accurate ISS for a Dynamically Reconfigurable Processor Architecture

19th IEEE International Parallel and Distributed Processing Symposium

... known as software pipelining[18], for highly parallel processor architectures (eg superscalar... more

A stream register file unit for reconfigurable processors

2006 IEEE International Symposium on Circuits and Systems

Abstract-This paper presents a local buffer memory in the of the available logic [11]. C/C++ or o... more

Single-cell microcentrifugation with monitored supernatant replacement in a microwell based device

Proceedings of Microtas …, 2011

We present here a lab-on-a-chip to perform cell centrifugation and re-suspension at single-cell l... more

Biocompatible Lab-On-Substrate technology platform

Electronic Components and Technology Conference, 2009

Multiwell plates in combination with optical inspection equipment are standard tools for biologic... more Multiwell plates in combination with optical inspection equipment are standard tools for biological and biomedical applications e.g. cell-to-cell interaction studies for cancer treatment. Microtechnology based multiwell plates have the potential to monitor physiological cellular interactions at single cell level with a high throughput e.g. for immunotherapy of cancer or targeted drug delivery, where each patient would receive drugs that are

Fast calibration of a dispenser nozzle for delivery of microdroplets over a flexible substrate

Sensors and Actuators A: Physical, 2011

In this work we present a method for delivering small fluid volumes possibly containing cells and... more

Inverted open microwells for cell trapping, cell aggregate formation and parallel recovery of live cells

Lab on a Chip, 2012

The inverted open microwell is a novel microstructure supporting isolation and trapping of cells,... more The inverted open microwell is a novel microstructure supporting isolation and trapping of cells, analysis of cell-cell and cell-molecule interactions and functional cell sorting. This work introduces the inverted open microwell concept, demonstrating successful isolation of K562 cells in 75 μm microwells fabricated on a flexible printed circuit board substrate, and recovery of viable cells onto standard microtiter plates after analysis and manipulation. Dielectrophoresis (DEP) was used during the delivery phase to control cell access to the microwell and force the formation of cell aggregates so as to ensure cell-cell contact and interaction. Cells were trapped at the air-fluid interface at the bottom edge of the open microwell. Once trapped, cells were retained on the meniscus even after DEP de-activation and fluid was exchanged to enable perfusion of nutrients and delivery of molecules to the microwell, as demonstrated by a calcein-staining protocol performed in the microsystem. Finally, cell viability was assessed on trapped cells by a calcein release assay and cell proliferation was demonstrated after multiple cells had been recovered in parallel onto standard microtiter plates.

Impedance measurement technique for high-sensitivity cell detection in microstructures with non-uniform conductivity distribution

Lab on a Chip, 2012

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Controlled Isolation and Patterning of K562 Leukemia Cells Using Electrically Activated Microchannels

Royal Chemical Society, 2011

Precise Positioning of Particles in Microwells within Horizontal DEP Cages

Download

A reconfigurable heterogeneous multiprocessor System on Chip

Reconfigurable IO Module

Abstract 3613: Lab-on-a-chip-based in-vitro functional profiling proves to be effective in predicting therapy outcome in AML patients

Cancer Research, 2018

Inter-Cell Networking Profiling Enables Comprehensive Characterization of Immune-Mediated Activity of Anti-CD38 Therapy through Ex-Vivo Analysis of Multiple Myeloma Patients

Blood, 2019

Ex-Vivo Drug Response Profiling for Precision Medicine Approaches in Acute Myeloid Leukemia with the Open Microwell Microfluidic Platform

Blood, 2016

Method and Apparatus for Manipulating Single Cells and Small Aggregates Thereof

A case-study on multimedia applications for the XiRisc reconfigurable processor

A powerful factor in the recent embedded systems market is undoubtedly the strong need to add por... more

A XiRisc-based SoC for embedded DSP applications

Proceedings of the IEEE 2004 Custom Integrated Circuits Conference (IEEE Cat. No.04CH37571)

A system level IP integration methodology for fast SOC design

Proceedings. 2003 International Symposium on System-on-Chip (IEEE Cat. No.03EX748)

Download

A Multi-Context Pipelined Array for Embedded Systems

2006 International Conference on Field Programmable Logic and Applications, 2006

ARCES (DEIS) University of Bologna, Bologna, Italy email: {andrea.lodi,cmucci}@deis.unibo. it {ac... more

Design and implementation of a reconfigurable heterogeneous multiprocessor SoC

IEEE Custom Integrated Circuits Conference 2006, 2006

A Cycle-Accurate ISS for a Dynamically Reconfigurable Processor Architecture

19th IEEE International Parallel and Distributed Processing Symposium

... known as software pipelining[18], for highly parallel processor architectures (eg superscalar... more

A stream register file unit for reconfigurable processors

2006 IEEE International Symposium on Circuits and Systems

Abstract-This paper presents a local buffer memory in the of the available logic [11]. C/C++ or o... more

Single-cell microcentrifugation with monitored supernatant replacement in a microwell based device

Proceedings of Microtas …, 2011

We present here a lab-on-a-chip to perform cell centrifugation and re-suspension at single-cell l... more

Biocompatible Lab-On-Substrate technology platform

Electronic Components and Technology Conference, 2009

Fast calibration of a dispenser nozzle for delivery of microdroplets over a flexible substrate

Sensors and Actuators A: Physical, 2011

In this work we present a method for delivering small fluid volumes possibly containing cells and... more

Inverted open microwells for cell trapping, cell aggregate formation and parallel recovery of live cells

Lab on a Chip, 2012

Impedance measurement technique for high-sensitivity cell detection in microstructures with non-uniform conductivity distribution

Lab on a Chip, 2012

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