Papers by Benjamín Fernández
Estudio de mercado y planteamiento económico de una inversión estratégica de supervivencia empresarial en una línea de recocido continuo
SE REALIZA UN ESTUDIO DE OFERTA Y DEMANDA EN EL MERCADO SIDERURGICO DE LOS PRODUCTOS DE HOJALATA ... more SE REALIZA UN ESTUDIO DE OFERTA Y DEMANDA EN EL MERCADO SIDERURGICO DE LOS PRODUCTOS DE HOJALATA Y CHAPA DE AUTOMOVILES Y ELECTRODOMESTICOS, CONSTATANDO LAS LIMITACIONES DEL RECOCIDO CONVENCIONAL EN CAMPANA, PARA HACER FRENTE A LA VARIACION CUALITATIVA DE LA DEMANDA, ORIENTADA HACIA MENORES ESPESORES Y MAYORES DUREZAS Y, SIMULTANEAMENTE, HACIA UNA MAYOR UNIFORMIDAD DE CARACTERISTICAS Y MEJOR ACABADO SUPERFICIAL. SE VALORA LA INTRODUCCION DE UNA NUEVA TECNOLOGIA DE RECOCIDO EN CONTINUO, COMO ESTRATEGIA COMPETITIVA PARA ASEGURAR LA PERMANENCIA EN UN MERCADO CAMBIANTE. SE ESTUDIAN LAS DIFERENTES ALTERNATIVAS POSIBLES DE INVERSION EN LINEAS DE RECOCIDO CONTINUO, PARA QUE LAS EMPRESAS SIDERURGICAS NACIONALES PUEDAN SEGUIR COMPITIENDO. SE ELIGE LA SOLUCION OPTIMA QUE ASEGURE EL MANTENIMIENTO DE LAS CUOTAS DE MERCADO Y FRENE EL CRECIMIENTO DE LAS IMPORTACIONES, CALCULANDO LOS PARAMETROS DE LA INVERSION, QUE SE REFUERZAN MEDIANTE UN ANALISIS DE SENSIBILIDAD.
PEEP e distribuzione topografica delle densità polmonari nella ARDS
Importance of Emotional Intelligence in the Professional Development of the Cand in Nursing
Glioarchitecture du cervelet de poulet. Observations préliminaires
Impact of rheumatoid arthritis on sexuality: adaptation and validation of the Qualisex questionnaire for use in Spain
Rheumatology International, 2021
Patients with rheumatoid arthritis (RA) have a significantly increased risk of sexual dysfunction... more Patients with rheumatoid arthritis (RA) have a significantly increased risk of sexual dysfunction. However, it is not properly included in commonly used questionnaires to assess health-related quality of life in RA. Qualisex is a questionnaire developed in France to assess the impact of RA on patients´ sexual function. Our aim was to adapt and validate this questionnaire for use with Spanish RA patients. Two independent translations and a backward translation were obtained. The final version was tested in a pilot study with 10 RA patients to detect any aspects that could hinder interpretation. The validity and reliability of the linguistically validated questionnaire were studied in a multicenter cross-sectional study, with a longitudinal component for reliability estimation. 125 RA patients were included. The response process, discrimination, internal consistency, internal structure, convergent validity (correlation with MGH-SFQ questionnaire, DAS-28, physician global assessment, patient global health assessment, RAID, HAQ, HADS and SF-12©) and reliability were analyzed. The inclusion of two extra items was proposed in the pilot study. The validity analysis detected responses for item 10 that were not coherent with responses for the rest of items. The Cronbach alpha coefficient was 0.971. The highest correlation (0.665) was obtained with MGH-SFQ (questionnaire measuring sexual functioning), followed by RAID (0.516). The intra-class correlation was 0.880 (95% CI 0.815; 0.923), higher than 0.85, which indicates excellent reliability. All parameters used to assess this questionnaire show highly acceptable values. Qualisex allows for a global score of RA patients’ sexual functioning and can be self-administered.
Intracellular protein complexes involved in synapse assembly in presynaptic neurons
Advances in Protein Chemistry and Structural Biology, 2019
The presynaptic active zone, composed of evolutionarily conserved protein complexes, is a special... more The presynaptic active zone, composed of evolutionarily conserved protein complexes, is a specialized area that serves to orchestrate precise and efficient neurotransmitter release by organizing various presynaptic proteins involved in mediating docking and priming of synaptic vesicles, recruiting voltage-gated calcium channels, and modulating presynaptic nerve terminals with aligned postsynaptic structures. Among membrane proteins localized to active zone, presynaptic neurexins and LAR-RPTPs (leukocyte common antigen-related receptor tyrosine phosphatase) have emerged as hubs that orchestrate both shared and distinct extracellular synaptic adhesion pathways. In this chapter, we discuss intracellular signaling cascades involved in recruiting various intracellular proteins at both excitatory and inhibitory synaptic sites. In particular, we highlight recent studies on key active zone proteins that physically and functionally link these cascades with neurexins and LAR-RPTPs in both vertebrate and invertebrate model systems. These studies allow us to build a general, universal view of how presynaptic active zones operate together with postsynaptic structures in neural circuits.
Evapotranspiración en viñas de secano sometidas a dos sistemas de manejo
Ciencia e investigación agraria, 1979
Restorative neurology and neuroscience, 2017
The present work examines α-synuclein expression in the nigrostriatal system of a rat chronic hep... more The present work examines α-synuclein expression in the nigrostriatal system of a rat chronic hepatic encephalopathy model induced by portacaval anastomosis (PCA). There is evidence that dopaminergic dysfunction in disease conditions is strongly associated with such expression. Possible relationships among dopaminergic neurons, astroglial cells and α-synuclein expression were sought. Brain tissue samples from rats at 1 and 6 months post-PCA, and controls, were analysed immunohistochemically using antibodies against tyrosine hydroxylase (TH), α-synuclein, glial fibrillary acidic protein (GFAP) and ubiquitin (Ub). In the control rats, TH immunoreactivity was detected in the neuronal cell bodies and processes in the substantia nigra pars compacta (SNc). A dense TH-positive network of neurons was also seen in the striatum. In the PCA-exposed rats, however, a reduction in TH-positive neurons was seen at both 1 and 6 months in the SNc, as well as a reduction in TH-positive fibres in the s...
International journal of cardiology, 2015
Crizotinib was the first clinically available inhibitor of the tyrosine kinase ALK, and next-gene... more Crizotinib was the first clinically available inhibitor of the tyrosine kinase ALK, and next-generation ALK inhibitors, such as alectinib, are now under development. Although crizotinib is generally well tolerated, severe esophageal injury has been reported as a rare but serious adverse event of crizotinib therapy. We now describe the successful treatment with alectinib of a patient who developed crizotinib-induced esophageal ulceration.
[Pseudomonas aeruginosa lung infections of senior patients in intensive care unit]
Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi, 2004
[Changes in the acid-base equilibrium in surgery of the abdominal aorta, effects of ventilation]
Minerva anestesiologica, 1990
Early degenerative changes in ganglionar neurons of toad
Evolution of Astrocytes in the Vertebrate CNS
Neuron-Glia Interrelations During Phylogeny
Evolution of Astrocytes in the Vertebrate CNS Isabel Suarez, Guillermo Bodega, Miguel Rubio, and ... more Evolution of Astrocytes in the Vertebrate CNS Isabel Suarez, Guillermo Bodega, Miguel Rubio, and Benjamin Fernandez 1. Introduction It has become more ... et al., 1985; Roots, 1986; Nona et al., 1989; Cardone and Roots, 1990; Monzon-Mayor et al., 1990; Cameron-Curry et al ...
Possible implication of ciliary neurotrophic factor (CNTF) and beta-synuclein in the ammonia effect on cultured rat astroglial cells: a study using DNA and protein microarrays
Neurochemistry international, 2006
Astrocytes are considered the key cell in hepatic encephalopathy; although their precise role in ... more Astrocytes are considered the key cell in hepatic encephalopathy; although their precise role in the disease has not yet been determined, exposure to ammonia appears to have an important pathogenic effect. We exposed confluent cultures of rat astroglial cells to ammonia (5mM NH(4)Cl) for 1, 3, 5 and 7 days, and determined astroglial levels of actin, glial fibrillary acidic protein (GFAP), glutamine synthetase (GS), GLAST glutamate transporter, 25kDa heat-shock protein (HSP25), HSP60 and HSP70 by Western blot; the glutamine content in culture medium was measured by mass spectrometry. Significant increases were observed for GS, HSP60 and glutamine, and significant reductions for actin and GFAP. Astrocytes exposed to ammonia for 4 days were used to analyze the effect of ammonia in protein and DNA microarrays. After protein microarray data filtration by signal intensity, x-fold change and z-score, 11 proteins were selected, among which the significant increase in beta-synuclein was conf...
Miscellanea on Encephalopathies - A Second Look, 2012
The Cerebellum, 2004
This paper reports the effects of pre-and perinatal exposure to Á 9-tetrahydrocannabinol (THC) on... more This paper reports the effects of pre-and perinatal exposure to Á 9-tetrahydrocannabinol (THC) on expression levels of speci®c AMPA glutamate receptor subunits (GluR1 and GluR2/3) in the cerebellum of male and female rats. Pregnant rats were administered saline or THC from gestational day 5 (ED5) to postnatal day 20 (PD20). Expression of the GluR1 and GluR2/3 subunits of AMPA glutamate receptors was analyzed by immunohistochemistry in THC-exposed rats at three postnatal ages: PD20 (still exposed to THC) to study the direct effect of drug exposure, and PD30 and PD70 (10 and 50 days following THC withdrawal) to analyze the long-term effects of prenatal exposure. Compared to controls, pre-and perinatal THC exposure decreased the immunoreactivity levels of the GluR1 subunit in Bergmann glial cells, as well as levels of the GluR2/3 subunit in Purkinje neurons at PD20. These changes in AMPA receptor subunit levels may correlate with the decreased excitatory neurotransmission described in the cerebellum after cannabinoid treatment, which could play a signi®cant role in the biochemical effects of THC. In addition, the reduced glutamate receptor expression observed at PD20 did not return to normal even after THC withdrawal (PD30 and PD70). The results support the idea that THC exposure during critical stages of cerebellar development may alter the glutamatergic system, not only during the drug exposure period itself but also in adults following THC withdrawal. The decreased expressions of glutamate receptors induced by developmental THC exposure could lead to functional alterations through the inhibition of glutamatergic neurotransmission, and clearly demonstrate an interaction between cannabinoids and the glutamatergic system.
Industrial Park Remediation in Aviles
Minerals & Energy - Raw Materials Report, 2005
Asturias has traditionally been a region with a primary industry based economy that, to a large e... more Asturias has traditionally been a region with a primary industry based economy that, to a large extent, developed around mining and the iron and steel industry. Consequently, the areas where such production activities were implemented have been subject to a high contamination potential, accentuated by the fact that the factories were located on quaternary soils, very sensitive to pollution, on which anthropic fill materials from the local industries were dumped. Over the years, old factories have been dismantled, leaving a legacy of contaminated soils, many of which, when released by the original production activity, have been occupied by new companies, as is the case with a large number of the existing industrial parks. In some cases the growth of the cities leads to an increasing demand for building land, with the result that the lands have been used for residential purposes. This situation is further aggravated by the limited land availability in the region, owing to a large extent to its abrupt orogra...
Metabolic Brain Disease, 2009
Hepatic encephalopathy (HE) is a neurologic disease associated with hepatic dysfunction. Astrogli... more Hepatic encephalopathy (HE) is a neurologic disease associated with hepatic dysfunction. Astroglial and neuronal alterations have been described in the basal ganglia in HE. Our study was performed to determine whether such alterations are mediated by nitric oxide (NO), by using an experimental model of HE (portacaval anastomosis [PCA]). The expression of the NO synthases (nNOS and iNOS) and the production of nitrotyrosine (NT) were evaluated in the striatum of rats exposed to PCA for 1 and 6 months. The expression of nNOS in the striatal neurons of PCA rats was increased compared to controls. nNOS expression was also detectable in astrocytes after 6 months of exposure to PCA. Whereas astroglial cells in the normal striatum showed no iNOS expression, iNOS was expressed in the astrocytes of PCA brains, mainly in perivascular processes at 6 months PCA exposure (demonstrated by colocalization with GFAP). The increased expression of both the nNOS and iNOS isoforms in PCA rats might indicate a critical role for NO in the pathomechanism of HE. To study the potential cell damage caused by NO, the deposition of NT in PCA-rats was analysed. Nitrotyrosine was detected in neurons although it was mainly seen in the astrocytes of PCA brains, in which double immunolabelling showed NT to be colocalized with GFAP. Thus, the present study shows the induction of iNOS and NT in astrocytes, which increases with the duration
Journal of Molecular Histology, 2009
Mitogen-activated protein kinases (MAPKs) are a superfamily of cytoplasmic serine/threonine kinas... more Mitogen-activated protein kinases (MAPKs) are a superfamily of cytoplasmic serine/threonine kinases that transduce many types of extracellular stimuli into cellular responses. p38MAPK is a member of this family with its active form in a diphosphorylated state (p38MAPKdiP). Two strong anti-p38MAPKdiP immunoreactive bands (apparent molecular weight 38 and 34 kDa) were detected by Western blotting in cultured astrocytes. Using a specific antibody and employing immunoprecipitation procedures and SELDI-TOF analysis, the 34 kDa band was found to correspond to Mxi2, a splice variant of p38MAPK; cultured astrocytes therefore express Mxi2. Separate protein extractions of different subcellular fractions, and fluorescent immunovisualisation employing confocal microscopy, showed Mxi2 to have a non-nuclear, cytosolic distribution in the studied cells. ERK1/2, protein whose intracellular distribution is influenced by Mxi2, showed the same cytoplasmic pattern than Mxi2.
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Papers by Benjamín Fernández