B. Desprez

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Ernst Pernicka

Universität Heidelberg

Alex Mauricio Jimenez-Ortega

Universidad Tecnológica del Chocó

Mahendra Maharjan

Tribhuvan University

Caroline J . Tully

University of Melbourne

Dr. Eluvathingal Antony Jayson

Kerala Forest Research Institute

Alejandro Valenzuela

Universidad Nacional de Tierra del Fuego

MARIA KRITSEPI-KONSTANTINOU

Aristotle University of Thessaloniki

Vedad Sakic

University of Sarajevo

Muhammad Mahmood Ul Hassan

University of Agriculture, Faisalabad, Pakistan

Mustafa Tayar

Uludag University

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Papers by B. Desprez

QTLS of seed nitrogen content in pea

HAL (Le Centre pour la Communication Scientifique Directe), Jun 7, 2004

L'Hétérosis et Son Utilisation en Amélioration Des Plantes et Des Animaux. Discussion

Valorisation Des Ressources Genetiques en Selection : Point De Vue D'Un Etablissement Obtenteur

Potential of genetic resources and breeding strategies for base-broadening in Beta

Broadening the Genetic Base of Crop Production

This chapter reviews topics on: the sugarbeet breeding research community; the genus Beta and its... more This chapter reviews topics on: the sugarbeet breeding research community; the genus Beta and its useful characters; bottlenecks to the utilization of Beta genetic resources; breeding approaches to broaden the genetic base of Beta (introgression from the primary gene pool; introgression from the tertiary gene pool; incorporation from the primary gene pool; incorporation from the secondary gene pool). The future role of gene banks in germplasm enhancement programme is also discussed.

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Preparation and fusion properties of protoplasts from mature pollen of Nicotiana tabacum

Plant cell reports, 1995

A method to remove the exine from mature tobacco pollen and to release numerous intact pollen pro... more A method to remove the exine from mature tobacco pollen and to release numerous intact pollen protoplasts has been developed. Post-anthesis binucleate pollen was treated with water, buffered with MES at pH 5.5, for two hours. Rupture of the exine was caused by the force of pollen hydration exposing the intine to subsequent enzymatic maceration. The high osmotic pressure (1000 mOsm·kg(-1) H2O) of pollen protoplasts required a special maceration medium, 4% KCl (w/v). Action of an enzyme solution containing 1% (w/v) Macerozyme and 1% (w/v) Cellulase gave rise to viable protoplasts within 4 hours. When cultured in a tobacco mesophyll protoplast culture medium, the pollen protoplasts underwent regeneration of a cell wall, formation of various tube-shaped structures, and division of the generative nucleus into two nuclei. Using a PEG/Ca(2+) method pollen protoplasts were fused with diploid mesophyll protoplasts. Evidence of transfer of chloroplasts into the pollen protoplasts was observed...

Regeneration and characterization of plants produced from mature tobacco pollen protoplasts via gametosomatic hybridization

Plant Cell Reports, 1995

Mature pollen protoplasts (n) isolated from kanamycin resistant plants of Nicotiana tabacum (2n =... more Mature pollen protoplasts (n) isolated from kanamycin resistant plants of Nicotiana tabacum (2n = 4x = 48) were fused with somatic mesophyll protoplasts (2n) of Nicotiana plumbaginifolia (2n = 20) to produce plants. A total of 3.6·10(6) mature pollen protoplasts were fused with 7·10(6) mesophyll protoplasts using a PEG/Ca(2+) method. Mature pollen protoplasts did not divide in our culture conditions, and N. plumbaginifolia protoplasts stopped dividing when the protoplast-derived colonies were transferred to a selection medium containing paromomycine (20 mg·l(-1)). A total of 133 actively growing colonies were recovered on the selection medium containing kanamycin (100 mg·l(-1)). Plants from twenty resulting cell lines were confirmed as hybrids (17) or cybrids (3) based on leaf and floral morphology and fertility analysis. Isozyme pattern analysis confirmed the nuclear hybrid and cybrid nature, respectively, for 2 and 3 typical gametosomatic selected plants. Root tip squashes of 6 of the gametosomatic hybrid plants revealed chromosome numbers ranging from 44 to 68; the 3 selected cybrid plants had 48 chromosomes. Evidence for organelle transmission from the mesophyll partner in the gametosomatic plants is shown. From the analysis it can be concluded that the gametosomatic fusion involving mature pollen protoplasts (n) carrying a dominant selection marker can be convenient for synthesis of either hybrids or cybrids. Such gametosomatic fusion is therefore considered as a new approach towards the production of androgenetic plants with a choosen cytoplasm.

Relationships among Cichorium species and related genera as determined by analysis of mitochondrial RFLPs

Theoretical and Applied Genetics, 1994

Mitochondrial DNA polymorphism was employed to assess cytoplasmic diversity among cytoypes of the... more Mitochondrial DNA polymorphism was employed to assess cytoplasmic diversity among cytoypes of the genus Cichorium and related genera of the tribe Lactuceae (Asteraceae). Hybridization patterns of total DNA using six restriction enzymes and five heterologous mtDNA probes were examined. From estimates of mtDNA diversity, Cichorium spinosum appeared as an ecotype of C. intybus rather than a separate species. Interspecific mtDNA polymorphism in the genus Cichorium was higher than that observed in Cicerbita Crepis, Lactuca and Tragopogon. Molecular data seemed to indicate that Catananche is very distant from the other genera examined. Intergeneric comparisons allowed the clustering of Cicerbita, Lactuca and Cichorium, genera which belong to different subtribes. However, further molecular investigations on a larger number of genera are needed to clarify the relationships among genera within and between subtribes of the tribe Lactuceae.

Developmental Genes Have Pleiotropic Effects on Plant Morphology and Source Capacity, Eventually Impacting on Seed Protein Content and Productivity in Pea

Plant Physiology, 2007

Increasing pea (Pisum sativum) seed nutritional value and particularly seed protein content, whil... more Increasing pea (Pisum sativum) seed nutritional value and particularly seed protein content, while maintaining yield, is an important challenge for further development of this crop. Seed protein content and yield are complex and unstable traits, integrating all the processes occurring during the plant life cycle. During filling, seeds are the main sink to which assimilates are preferentially allocated at the expense of vegetative organs. Nitrogen seed demand is satisfied partly by nitrogen acquired by the roots, but also by nitrogen remobilized from vegetative organs. In this study, we evaluated the respective roles of nitrogen source capacity and sink strength in the genetic variability of seed protein content and yield. We showed in eight genotypes of diverse origins that both the maximal rate of nitrogen accumulation in the seeds and nitrogen source capacity varied among genotypes. Then, to identify the genetic factors responsible for seed protein content and yield variation, we ...

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In situ gynogenetic haploid plants of chicory (Cichorium intybus L.) after intergeneric hybridization with Cicerbita alpina Walbr

Plant Cell Reports, 1996

In situ gynogenetic haploid #ants of chicory (Cichorium intybus L.) after intergenerie hybridizat... more

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Taxonomic, spatial and adaptive genetic variation of Beta section Beta

Theoretical and Applied Genetics, 2015

We investigated in deeper detail the genetic structure of B. vulgaris subsp. maritima, which cons... more We investigated in deeper detail the genetic structure of B. vulgaris subsp. maritima, which constituted the majority (80 %) of the wild samples. This subspecies exhibited a clinal genetic variation from SouthEast to NorthWest. We detected some markers significantly associated to environmental variables in B. vulgaris subsp. maritima. These associations are interpreted as results of natural selection. The variable most often involved in the associations was annual mean temperature. Therefore, these markers can be useful for the development of frost-tolerant winter beets and drought-tolerant rain-fed beets.

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Identifying the Best Sulfonylurea Herbicide for Weed Control and Crop Tolerance in Sulfonylurea-resistant Chicory (Cichorium Intybus)

Developmental Genes Have Pleiotropic Effects on Plant Morphology and Source Capacity, Eventually Impacting on Seed Protein Content and Productivity in Pea

by Pascal Marget and B. Desprez

PLANT PHYSIOLOGY, 2007

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Taxonomic, spatial and adaptive genetic variation of Beta section Beta

by Pierre Devaux and B. Desprez

Theoretical and Applied Genetics, 2015

The genetic variation of Beta section Beta is structured into four taxonomic and spatial clusters... more The genetic variation of Beta section Beta is structured into four taxonomic and spatial clusters. There are significant associations between molecular markers and environmental variables. We investigated the genetic diversity of Beta section Beta, which includes the wild and cultivated relatives of the sugar beet. The taxa included in the study were: Beta vulgaris subsp. maritima, B. vulgaris subsp. adanensis, B. macrocarpa, B. patula and B. vulgaris subsp. vulgaris (garden beet, leaf beet and swiss chards). We collected 1264 accessions originating from the entire distribution area of these taxa and genotyped them for 4436 DArT markers (DArTs). We showed that the genetic variation of these accessions is structured into four taxonomic and spatial clusters: (1) samples of Beta macrocarpa, (2) samples of Beta vulgaris subsp. adanensis, (3) Mediterranean and Asian samples and (4) Atlantic and Northern European samples. These last two clusters were mainly composed of samples of Beta vulgaris subsp. maritima. We investigated in deeper detail the genetic structure of B. vulgaris subsp. maritima, which constituted the majority (80 %) of the wild samples. This subspecies exhibited a clinal genetic variation from South-East to North-West. We detected some markers significantly associated to environmental variables in B. vulgaris subsp. maritima. These associations are interpreted as results of natural selection. The variable most often involved in the associations was annual mean temperature. Therefore, these markers can be useful for the development of frost-tolerant winter beets and drought-tolerant rain-fed beets.

QTLS of seed nitrogen content in pea

HAL (Le Centre pour la Communication Scientifique Directe), Jun 7, 2004

L'Hétérosis et Son Utilisation en Amélioration Des Plantes et Des Animaux. Discussion

Valorisation Des Ressources Genetiques en Selection : Point De Vue D'Un Etablissement Obtenteur

Potential of genetic resources and breeding strategies for base-broadening in Beta

Broadening the Genetic Base of Crop Production

Download

Preparation and fusion properties of protoplasts from mature pollen of Nicotiana tabacum

Plant cell reports, 1995

Regeneration and characterization of plants produced from mature tobacco pollen protoplasts via gametosomatic hybridization

Plant Cell Reports, 1995

Relationships among Cichorium species and related genera as determined by analysis of mitochondrial RFLPs

Theoretical and Applied Genetics, 1994

Developmental Genes Have Pleiotropic Effects on Plant Morphology and Source Capacity, Eventually Impacting on Seed Protein Content and Productivity in Pea

Plant Physiology, 2007

Download

In situ gynogenetic haploid plants of chicory (Cichorium intybus L.) after intergeneric hybridization with Cicerbita alpina Walbr

Plant Cell Reports, 1996

In situ gynogenetic haploid #ants of chicory (Cichorium intybus L.) after intergenerie hybridizat... more

Download

Taxonomic, spatial and adaptive genetic variation of Beta section Beta

Theoretical and Applied Genetics, 2015

Download

Identifying the Best Sulfonylurea Herbicide for Weed Control and Crop Tolerance in Sulfonylurea-resistant Chicory (Cichorium Intybus)

Developmental Genes Have Pleiotropic Effects on Plant Morphology and Source Capacity, Eventually Impacting on Seed Protein Content and Productivity in Pea

by Pascal Marget and B. Desprez

PLANT PHYSIOLOGY, 2007

Download

Taxonomic, spatial and adaptive genetic variation of Beta section Beta

by Pierre Devaux and B. Desprez

Theoretical and Applied Genetics, 2015

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