Papers by Axel Alonso Rojas Fernández
The aim of this study is to examine how climate change is framed in Swedish climate reporting, pu... more The aim of this study is to examine how climate change is framed in Swedish climate reporting, published on the website of national newspapers – Dagens Nyheter, Svenska Dagbladet, Aftonbladet and Expressen – from September 2018 throughout August 2019. Through a quantitative content study regarding climate change published online in chosen newspapers, the goal is to map out how Swedish newspapers use frames – consciously or not – to affect the public. Through a perspective of the framing theory – invented and coined by Erving Goffman in the 1970’s – questions about frames are asked while reading chosen articles on climate change, to see which frames are most prominent. From previous scientific studies, it is suggested that certain frames in the news-media has certain effects on the readers. This study may hopefully contribute to the debate on how news in media are affecting the view on the climate change in Sweden. The findings of the study show that the structural responsibility fra...
IFMBE Proceedings, 2010
Doxorubicin (DOX) is an anthracycline drug widely used in the treatment of a large spectrum of so... more Doxorubicin (DOX) is an anthracycline drug widely used in the treatment of a large spectrum of solid tumors. Its application is limited by its efflux through the multidrug resistance (MDR) protein. In this study, we explored the potential of DOX - PLGA nanoparticles (DNPs) and antibody decorated nanoparticles (ADNPs) to overcome MDR in cancer cells. DNPs were prepared by the O/W emulsion solvent evaporation method. The surface decoration of nanoparticles with antibody was performed via carbodiimide reaction. The particles were characterized for their size and zeta potential, and their in vitro uptake was compared with their unconjugated counterparts. Entrapment efficiency of DNPs and ADNPs was measured by fluorescence using the DMSO burst release procedure. Cytotoxicity was measured using the SRB assay. The DNP and ADNP nanoparticles had average diameter of 162.6 ± 2.0 nm and 213.0 ± 3.5 nm respectively. Their corresponding surface charges were -13.2 ± 2.3 mV and -1.3 ± 3.8 mV respectively. Our results showed that cellular uptake of DOX from DNPs in DOX-resistant MES-SA/Dx5 cancer cell was higher compared to free DOX. However, the uptake of DOX from DNPs in MES-SA and SKOV-3 cancer cell lines was comparable to free DOX treatment. Next, we conjugated the DNPs with HER-2 antibody to specifically target to SKOV-3 cancer cell line and MES-SA and MES-SA/Dx5 were used as negative controls. Results showed higher uptake of DOX from ADNPs compared to free DOX and DNPs in SKOV-3. However, the cellular uptake of DOX from ADNPs was comparable to DNPs in MES-SA and MES-SA/Dx5. Cytotoxicity results were consistent with the cellular uptake data. Our study concludes that the targeted DNPs may enhance the cellular uptake and cytotoxicity in SKOV-3.
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Papers by Axel Alonso Rojas Fernández