Papers by Armen Nersesyan
BMC Pharmacology, Nov 1, 2007
Праці Наукового товариства імені Шевченка, Dec 30, 2022
Introduction. The incidence of post-traumatic stress disorder (PTSD) is increasing due to war in ... more Introduction. The incidence of post-traumatic stress disorder (PTSD) is increasing due to war in Ukraine is of growing importance as this disorder is a serious risk factor for alcohol/drug abuse, depression, suicide and functional dysregulation of internal organs, in particular cardiovascular, endocrine, immune systems. PTSD can also lead to social inadaptation in family and at work. This makes PTSD a very important social problem in Ukraine that causes significant economic damage, since most of the PTSD patients are young and in working age. Objectives. The purpose of this paper is to describe the rationale, key elements of design, methodology and future prospects of the ongoing project "Exploratory study of health related physiological and biochemical parameters to identify simple, robust and relevant biomarkers for therapeutic interventions for PTSD patients". Methods. Short time ECG records (5 minutes in supine position and 6 minutes in orthostatic test) recorded by "CARDIOLAB" (Kharkiv, Ukraine) digital ECG device with software to analyze HRV was used to calculate time-domain and frequency-domain
Mutagenesis, Aug 8, 2012
Circulating unconjugated bilirubin (UCB) has been reported to protect against lung and colorectal... more Circulating unconjugated bilirubin (UCB) has been reported to protect against lung and colorectal cancer. The present study aimed to explore, for the first time, whether mildly elevated circulating UCB, as found in Gilbert`s syndrome (GS), is associated with changes of DNA damage. A random 76 individuals, matched for age and gender, were recruited from the general population and allocated into the GS group (UCB ≥17.1 µM; n = 38) or control group (UCB <17.1 µM; n = 38). Chromosomal and cytological changes were determined in lymphocytes and buccal cells using the cytokinesis-block micronucleus cytome assay (CBMN) and buccal micronucleus cytome assay (BMcyt). No significant differences were found between GS subjects and the control group in the CBMN and BMcyt determined endpoints. Subsequently, when age dependency of effects were analysed, lower formation of buccal micronucleated cells (by 73.3%) and buccal nuclear buds (by 70.9%) in the GS subgroup ≥30 years were found, compared to the GS subgroup <30 years. These findings suggest DNA protection in epithelial tissue of older individuals with GS.
Scientific Reports
Consumption of very hot beverages and foods increases the incidence of oral and esophageal cancer... more Consumption of very hot beverages and foods increases the incidence of oral and esophageal cancer but the mechanisms are not known and the critical temperature is not well defined. We realized a study with exfoliated cells from the oral cavity of individuals (n = 73) that live in an area in Iran which has the highest incidence of EC worldwide. Consumption of beverages at very high temperatures is a characteristic feature of this population. We analyzed biomarkers which are (i) indicative for genetic instability (micronuclei that are formed as a consequence of chromosomal damage, nuclear buds which are a consequence of gene amplifications and binucleated cells which reflect mitotic disturbances), (ii) markers that reflect cytotoxic effects (condensed chromatin, karyorrhectic, karyolitic and pyknotic cells), (iii) furthermore, we determined the number of basal cells which is indicative for the regenerative capacity of the buccal mucosa. The impact of the drinking temperature on the fr...
We analyzed the incidence of male breast cancer in Armenia over the period of 20 years (1980-2000... more We analyzed the incidence of male breast cancer in Armenia over the period of 20 years (1980-2000). The obtained data showed that, unlike female BC, male BC incidence also varies in Armenia as in most developed and developing countries, but without any regularity. The mean crude rate for the observed period is lower than in developed but comparable with developing countries. The most important peculiarity of male BC in Armenia is young age of patients.
Issues in Toxicology, 2019
Formaldehyde (FAL) is a Class I carcinogen. Occupational exposure to this chemical is not uncommo... more Formaldehyde (FAL) is a Class I carcinogen. Occupational exposure to this chemical is not uncommon and there is a need to validate appropriate methods for detecting its genotoxic effects in vivo in humans. One of the most commonly used methods to measure the genotoxic effects of exposure to environmental chemicals is the lymphocyte cytokinesis-block micronucleus (L-CBMN) assay. We, therefore, performed a systematic review and statistical analysis of the results from all published reports (N = 17 studies) in which the L-CBMN assay was used to measure the genotoxic effects of human exposure to FAL. The results of this systematic review indicate that the majority (62%) of these studies showed significant increases in lymphocyte micronuclei (MN), a biomarker of chromosome breakage or loss, in exposed subjects relative to controls. The results of all studies (positive or negative), when pooled together, indicated a highly significant doubling in lymphocyte MN frequency in those exposed t...
Cytology and Genetics, 2007
Clinical Oral Investigations, 2019
Journal of Clinical Oncology, 2005
725 Background: Survival of young patients with breast cancer is poor. Although, research shows n... more 725 Background: Survival of young patients with breast cancer is poor. Although, research shows negative influence of a significant number of pathological factors on survival of breast cancer patients, there is no clear impact of presence of cancer emboli in blood vessels around tumor on survival of patients with premenopausal breast cancer, especially young patients with breast cancer.The aim of this study is to determine the impact of presence of cancer emboli in blood vessels around tumor on survival of young patients compared with middle age group. Material and method Study involves 2 groups of patients with stages II-III of premenopausal breast cancer. 375 patients were young age (20–35 years old), and 380 -middle age (36–45 y.o).Cancer emboli in blood vessels around tumor were analyzed on surgical specimens after mastectomy. 5 and 10- year survival was established by “ life table” Method: Comparative analysis of 5- and 10- year survival rates done under influence of presence or absence of cancer emb...
Cell and Tissue Biology, 2008
Redox biology, Jan 3, 2015
Sedentary lifestyle is a major risk factor for diabetes, cardiovascular and many other age-relate... more Sedentary lifestyle is a major risk factor for diabetes, cardiovascular and many other age-related diseases. Heart rate variability (HRV) reflects the function of regulatory systems of internal organs and may sensitively indicate early metabolic disturbances. We hypothesize that quantitative and qualitative changes of HRV in young subjects may reflect early metabolic derangements responsible for further development of clinically significant disease. The aim of our study was to determine whether the parameters of carbohydrate metabolism (fasting blood glucose, HBA1c and surrogate insulin sensitivity/resistance indices) correlate with anthropometric data and HRV. The study group consisted of 30 healthy sedentary male subjects aged 20-40, nonsmokers, mainly office and research employees, medical staff and students. Athletes, actively training more than one hour per week, severely obese and men of physical work were excluded from the study. HRV parameters were derived from short term EC...
International Journal of Occupational Medicine and Environmental Health, 2012
Scandinavian Journal of Work, Environment & Health, 2001
Mutagenesis, 2009
Silybin (SB), a constituent of the medicinal plant Silybum marianum, is reported to be a potent h... more Silybin (SB), a constituent of the medicinal plant Silybum marianum, is reported to be a potent hepatoprotective agent, but little is currently known regarding its genotoxicity, mutagenicity and potential chemopreventive properties. In this study, we evaluated the ability of SB to induce DNA migration and micronuclei (MN) formation in human hepatoma cells (HepG2). Also, possible preventive effects of SB on MN formation induced by three different mutagens, bleomycin (BLEO), benzo[a]pyrene (B[a]P) and aflatoxin B 1 (AFB 1 ), were studied. To clarify the possible mechanism of SB antimutagenicity, three treatment protocols were applied: pretreatment, in which SB was added before the application of the mutagens; simultaneous treatment, in which SB was added during treatment and post-treatment, in which SB was added after the application of the mutagens. At concentrations up to 100 mM, SB was nongenotoxic, while at a concentration of 200 mM, SB induced DNA migration, generated oxidized DNA bases, reduced cell viability, decreased the replicative index of the cells and induced oxidative stress. It is noteworthy that SB was able to reduce the genotoxic effect induced by B[a]P, BLEO and AFB 1 in pretreatment and simultaneous treatments but had no significant effect on DNA damage induction in posttreatment. Taken together, our findings indicate that SB presents anti-genotoxic activity in vitro, which suggests potential use as a chemopreventive agent.
JNCI Journal of the National Cancer Institute, 1998
JNCI Journal of the National Cancer Institute, 2001
JNCI Journal of the National Cancer Institute, 2001
CancerSpectrum Knowledge Environment, 2002
International Journal of Hygiene and Environmental Health, 2014
Welders are inhalatively exposed to fumes which contain genotoxic carcinogens and it was found in... more Welders are inhalatively exposed to fumes which contain genotoxic carcinogens and it was found in epidemiological studies that they have increased cancer rates which may be causally related to DNA damage. In order to assess their health risks and to find out which chemicals cause the adverse effects, bioassays can be performed which enable the detection of genetic damage. The aim of the present study was a comparative investigation with exfoliated buccal and nasal cells in regard to induction of chromosomal alterations and acute cytotoxicity in welders and unexposed controls (n = 22 per group). To elucidate the factors which account for genotoxic and cytotoxic effects, additional biochemical parameters were monitored reflecting the redox status as well as concentrations of different metals and 1-hydroxypyrene (1-OHP) in body fluids. We found in the nasal cells significant induction of alterations which are indicative for DNA damage, i.e. of micronuclei (MNi) and nuclear buds, while elevated rates of nuclear anomalies reflecting cytotoxic effects (condensed chromatin, karyorrhexis, karyolylsis) were detected in cells from both organs. The levels of certain metals (Cr, Cu, Mn, Mo, Ni), but not markers of oxidative damage were significantly higher in the body fluids of the welders. Multivariate Poisson regression analyses indicate that exposure to Mo (15% MNi increase by one standard deviation increase of Mo in serum), Ni (9% increase) and Mn (14% increase) are positively associated with the induction of MNi in nasal cells while Ni was associated with cytotoxic effects in both types of cells (12 and 16% increase). Taken together, our findings indicate that epithelial cells from the respiratory tract are suitable for the detection of DNAdamaging and cytotoxic effects in welders and can be used to assess health risks associated with genomic instability.
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Papers by Armen Nersesyan