Papers by Antonio Lalusin
Philippine Journal of Crop Science, 2011
Philippine Journal of Crop Science, 2013
Science Diliman, 2017
Fifty-seven hibiscus hybrid progenies from different crosses were characterized and evaluated for... more Fifty-seven hibiscus hybrid progenies from different crosses were characterized and evaluated for morphological traits to select hybrids with unique color and form. A total of 14 progenies with the following pedigrees were selected: 22xDT-9, (LLxEFA)xGC-2, (LLxEFA)xGC-8, DSxGC-7, 20xGC-5, (GCxBGB)xHP-4, GCxDS-4, ABAxMDM-1, ABAxMDM-3, 23xGC-2, CVxNB-1, CVxNB-2, CVxMP-4 and CVxNB-6. Phenotypic data were analyzed for principal component analysis (PCA) and agglomerative cluster analysis. Correlation using PCA revealed signif icant positive association between flower size and leaf size, and between petiole length and leaf size. PCA depicted three major PCs with eigenvalue >1 contributing 78% of the total cumulative variability among different hybrids. The PC-I showed positive factor loadings for all the traits. The contribution of flower size, leaf size and style length was highest in PC-I. Cluster analysis grouped the 57 hybrids into f ive clusters. Cluster-I had the highest number o...
Thesis (Ph. D. in Bioresource Engineering)--University of Tsukuba, (A), no. 4011, 2006.3.24 Inclu... more Thesis (Ph. D. in Bioresource Engineering)--University of Tsukuba, (A), no. 4011, 2006.3.24 Includes bibliographical references
Scientia Horticulturae, Dec 1, 2004
... et al., 1996) and is the most polymorphic microsatellites in the rice species ( Akagi ... wit... more ... et al., 1996) and is the most polymorphic microsatellites in the rice species ( Akagi ... with other authors' conclusion as established by RAPD, RFLP and ISSR analysis (Jarret et ... gene flow among genotypes, while vegetative reproduction maintains the high level of genetic diversity. ...
Philippine Journal of Crop Science, 2011
Philippine Journal of Crop Science, 2017
Breeding Science, 2003
Inter-simple sequence repeat (ISSR) amplification was evaluated for its usefulness in generating ... more Inter-simple sequence repeat (ISSR) amplification was evaluated for its usefulness in generating DNA markers for sweetpotato and related wild species. ISSR markers were obtained through PCR amplification using simple sequence repeat (SSR) primers. Optimization of the reaction conditions was successfully achieved for 24 % out of 100 SSR primers screened. The functional primers included anchored and unanchored primers. All of the anchored primers used were dinucleotide repeat motifs. The unanchored primers consisted of tri-, tetra-and penta-nucleotide repeat motifs. Eight primers were selected and employed to assess the genetic diversity and relationship between 34 accessions of sweetpotato and its related wild species. The ISSR markers are highly polymorphic among the taxa studied. Several sweetpotato accessions were clustered together based on their geographic origin. The mode of inheritance of the ISSR markers was analyzed in a pseudo-test cross mapping population. Twenty-two SSR primers amplified 70 reproducible polymorphic ISSR markers and Mendelian segregation of polymorphic bands was demonstrated. Among the 70 ISSR markers, simplex and duplex markers accounted for 70 % and 15.7 %, respectively. One linkage between two simplex ISSR markers was found. Thus, the ISSR markers could be suitable for fingerprinting of cultivated sweeptotato and its related wild species and for constructing a linkage map of sweetpotato.
Philippine Journal of Crop Science, 2014
This article is an open access article distributed under the terms and conditions of the Creative... more This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY
Philippine Journal of Crop Science, 2015
Philippine Agricultural Scientist, 2021
In barley, breeding using good genetic characteristics can improve the quality or quantity of cro... more In barley, breeding using good genetic characteristics can improve the quality or quantity of crop characters from one generation to the next generation. The development of effective molecular markers in barley is crucial for understanding and analyzing the diversity of useful alleles. In this study, we conducted genetic relationship analysis using expressed sequence tag-simple sequence repeat (EST-SSR) markers for barley identification and assessment of barley cultivar similarity. Seeds from 82 cultivars, including 31 each of naked and hulled barley from the Korea Seed and Variety Service and 20 of malting barley from the RDA-Genebank Information Center, were analyzed in this study. A cDNA library of the cultivar Gwanbori was constructed for use in analysis of genetic relationships, and 58 EST-SSR markers were developed and characterized. In total, 47 SSR markers were employed to analyze polymorphisms. A relationship dendrogram based on the polymorphism data was constructed to compare genetic diversity. We found that the polymorphism information content among the examined cultivars was 0.519, which indicates that there is low genetic diversity among Korean barley cultivars. The results obtained in this study may be useful in preventing redundant investment in new cultivars and in resolving disputes over seed patents. Our approach can be used by companies and government groups to develop different cultivars with distinguishable markers. In addition, the developed markers can be used for quantitative trait locus analysis to improve both the quantity and the quality of cultivated barley.
Philippine Journal of Crop Science, 2019
Philippine Journal of Crop Science, 2016
Acta horticulturae, Nov 1, 2017
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Papers by Antonio Lalusin