Papers by Antonio Coutinho
Annual Review of Immunology, 1984
Revista da Universidade Federal de Minas Gerais, 2016
Sobre Ciência e o como fazer, aprendi tudo dos meus mestres, Göran Möller e Niels Jerne; aprendi ... more Sobre Ciência e o como fazer, aprendi tudo dos meus mestres, Göran Möller e Niels Jerne; aprendi exatamente as mesmas coisas duas vezes, para não me esquecer nunca mais. Com Tomaz Mota-Santos e Nelson Vaz, o meu caminho já é longo; e muito ganhei em sapiência e bondade com os repetidos exemplos queeles me deram. Bem hajam.
Annales de l'Institut Pasteur / Immunologie, 1984
A grain of polemics in scientific discussion is the salt of intellectual exchanges. If science pr... more A grain of polemics in scientific discussion is the salt of intellectual exchanges. If science progresses by substituting prevalent convictions with new competing hypotheses, controversies are a sign of good health in a discipline. I believe there are not many heated discussions on basic issues of geography or zoology. By raising small contradictions to the dignity of paradoxes, polemic treatment of the issues radicalizes and clarifies them. Few aspects of cellular immunology have been so controversial over the last ten years as the question of MHC restriction in T/B cell collaboration. With the introduction to the subject drawn by Ratcliffe and Mitchison, I need to spend little time in justifying the importance of the question. The complexity of the problem is documented by the majority of the contributors who wished to consider it together with general aspects of B-cell activation (particularly the role of Ig receptors) and the other side of T/B cell collaboration, namely, T-cell activation by B lymphocytes. More important, the solution to this question will determine the validity of hypotheses on self-nonself discrimination and on the selection of available repertoires. Clearly, discrepancies among the specialists still exist and no consensus cb-uld be reached at the present time. Rather, some (strong) statements spelled out by the contributors raise new incongruencies in currently accepted points. Zubler's contention that ~ antigens (of the T-dependent type) or anti-Ig antibodies alone do not render the resting B cells responsive to any non-specific T-cell factors ,, is perfectly sensible in the context of restricted T/B interactions, but questions a large number of observations. Given, however, the troubled history of B-cell stimulation by anti-Ig antibodies (from the original failures to the widely confirmed competence of purified antibodies to single-handedly activate virtually pure populations of B cells into extensive proliferation, and to the present and unanimous
Research in Immunology, 1989
Annales De L Institut Pasteur Immunologie, 1981
European Journal of Immunology, 1985
Current Topics in Microbiology and Immunology, 1982
This paper sums up the impact of 3 most stimulating days at the Institute for Virology and Immuno... more This paper sums up the impact of 3 most stimulating days at the Institute for Virology and Immunobiology in Wurzburg upon my immunological convictions and my ignorance of retroviruses. I will first be concerned with some general aspects of lymphocyte activation and growth that I consider fundamental for framing questions in the correct perspective. I will then consider, on a purely speculative basis, possible implications of some experimental findings presented at the workshop and reviewed in this volume that define peculiarities of viral-induced lymphocyte growth. To do so, I will have to interpret other’s results without attempting to engage in any critical appreciation of them. The value of such speculations is, therefore, determined by the validity of the original results.
Scandinavian Journal of Immunology, 2006
During screening of T‐T hybridomas for B‐cell‐stimulating factors (BSF) we found in the condition... more During screening of T‐T hybridomas for B‐cell‐stimulating factors (BSF) we found in the conditioned medium of such a line (TUH‐U) an activity that turned out to cosediment with a contaminating strain of Mycoplasma arginini on centrifugation. Since it only affected B blasts and only to a minor extent small cells and since it did not induce immunoglobulin secretion, the mycoplasma strain seemed to mimic a BSF. M. arginini was also found in two other T‐T hybridoma producer lines but not in a clone of the EL‐4 line. Three other M. arginini isolates were not stimulatory for B cells. The presented results point towards the importance of careful control for mycoplasma contamination in established cell lines.
Scandinavian Journal of Immunology, 2008
Göran Möller, one of the forefront immunologists in Sweden and Scandinavia, has died at the age o... more Göran Möller, one of the forefront immunologists in Sweden and Scandinavia, has died at the age of 71 years. Göran studied medicine at the Karolinska Institutet in Stockholm, Sweden. There, at the Department of Tumorbiology under the head of George Klein, he started his scientific career. At this time, he also met his wife, Erna. Together with her, the Möllers became one of the most well-known and respected international research couples of our time. Göran's career went through the Serafimerlasarettet hospital in downtown Stockholm to the Wallenberg laboratory and later on to the Department of Immunology at the Stockholm University. At the latter two laboratories, he laid the foundations for a most successful scientific career and a unique mentorship. His scientific achievements are described in this issue of the Scandinavian Journal of Immunology. His last years were spent as an emeritus professor at the Karolinska Institutet. Göran was not only recognized as a leading immunologist and scientific mentor, but was also instrumental for development of immunology in Scandinavia. Göran was fundamental in starting up the Scandinavian Society for Immunology (SSI). In the late 1960s, societies for immunology were founded in many countries and plans for the foundation of an International Union of Immunological Societies had developed. Pressure was also on the Scandinavian countries to form immunological societies. The intense discussions that took place during 1967 and 1968, with Göran as one of the forefront actors, led to the conclusion that a Scandinavian Society for Immunology should be formed. On the basis of these discussions, steps were taken to invite immunologists from all the Nordic countries to an inaugurating meeting. In March 1969, Göran arranged a meeting in the Wennergren Center. Besides a scientific programme, this meeting had on its agenda the foundation of the 'Scandinavian Society for Immunology' (SSI). Annual meetings of the Society then followed, and are still arranged every year. Göran took an active part in numerous of these SSI meetings. He also arranged two meetings. Notably, the 1990 Stockholm meeting, with Göran as a Meeting Secretary, gathered more than 900 delegates. Göran Möller also played an important role for the Scandinavian Journal of Immunology, which was established in 1972, and since 1975 has been the official journal of the SSI. From 1989 to 2002, he was one of the three managing editors and continued as an associate editor and later on the senior advisory board until his death. Göran and his group contributed many of their original scientific papers to the journal. He was also an active spokesman for the close collaboration between the SSI and the Journal. Over the years, this close collaboration has also brought substantial economical support from the journal to various activities of the SSI. Göran became a honorary member of the SSI in 1989. Göran was certainly one of the most influential immunologists in Scandinavia, well respected for his vast knowledge, his sharp brain and his unique personality. As evident from above, he was instrumental in founding the SSI and played a seminal role for the development of the Scandinavian Journal for Immunology. He will be remembered for long among many of us.
Journal of Theoretical Biology, 1991
Immunological Reviews, 1975
The extensive discussion of the model and the various competing concepts or statements, as well a... more The extensive discussion of the model and the various competing concepts or statements, as well as the exhaustive presentation of the experimental facts which support it, have been dealt with in detail elsewhere (Coutinho & Moller 1974, Coutinho et al. 1974, Coutinho & Moller 1975). I will now restrict myself to the outline of the theory behind the model, and to some of its implications. It is useless to say that the model was built up together with Dr. Goran Moller, and that I shared with him and with Dr. Eva Gronowicz the elaboration of many of the concepts presented here.
Immunological Reviews, 1975
... 50, Sweden. Publication History. Issue published online: 28 APR 2006; Article first published... more ... 50, Sweden. Publication History. Issue published online: 28 APR 2006; Article first published online: 28 APR 2006. SEARCH. Search Scope ... SEARCH BY CITATION. Volume: Issue: Page: ... fluorescent antibody. J. exp. Med. 91, 1 ...
European Journal of Immunology, 2006
CD4+CD25+Foxp3+ regulatory T cells (Treg) are natural suppressors of autoimmunity but they can al... more CD4+CD25+Foxp3+ regulatory T cells (Treg) are natural suppressors of autoimmunity but they can also dampen the effective clearance of infectious organisms. These cells have the potential to be exploited to prevent transplant rejection and to treat autoimmune disease. A paper in this issue of the European Journal of Immunology details a method to selectively expand antigen‐specific Treg from a polyclonal Treg population, by using a specific dendritic cell (DC) subset. Furthermore, the authors show that such Treg can be used to prevent experimental type I diabetes; however, as Treg are positively selected by thymic epithelial cells (TEC) on the basis of self‐reactivity, they would systematically suppress protective immune responses unless their repertoire is devoid of recognition towards peripheral antigen‐presenting cells. This may be achieved by negative selection of developing Treg on thymic DC, thus creating a ‘blind‐spot’ corresponding to DC‐self‐antigens in the mature Treg reper...
European Journal of Immunology, 1981
The frequency of lipopolysaccharide (LPS)-reactive B cells developing into clones that secrete th... more The frequency of lipopolysaccharide (LPS)-reactive B cells developing into clones that secrete the various immunoglobulin (Ig) classes has been determined in vitro, in cells from BALB/c mice, under culture conditions which detect all growth-inducible cells. Secretion of the different Ig classes was assessed in the protein A plaque assay for Ig-secreting, plaque-forming cells by using developing antisera specific for either IgM, IgG1, IgG2a, IgG2b, IgG3 or IgA. In all lymphoid organs tested (spleen, bone marrow, mesenteric lymph nodes and thoracic duct), a considerable proportion of all B cells (5-20%) was induced by LPS to yield a clone of IgM-secreting cells. Frequency determinations of LPS-reactive cells giving rise to descendants secreting other Ig isotypes revealed that, on an average, and irrespective of the origin of the cells, 7% of all IgM-secreting clones switched to the synthesis of IgG1, 39% to IgG2, 41% to IgG3 and 1% to IgA. Roughly the same frequencies of B cells switching CH gene expression were found among spleen cells of athymic nude mice. No correlation was found between the clonal frequencies of CH gene expression in polyclonally activated B cells and the in vivo "background" Ig-secreting cells suggesting that the CH gene expression in B cells is influenced by the quality of stimulation and other regulating influences.
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Papers by Antonio Coutinho