Papers by Antoni Aguilella
About the presence of the Broad-leaved Spindle (Celastraceae) in the Valencian Community. This pa... more About the presence of the Broad-leaved Spindle (Celastraceae) in the Valencian Community. This paper deals with the occurrence of the Broad-leaved Spindle Euonymus latifolius (L.) Miller in two localities of the Valencian Community (E Spain). In one of these localities (Alpuente, Valencia) the plant is believed to be extinct, while the second one (El Toro, Castellón) would be the only confirmed location in the Valencian Community.
International Journal of Plant Sciences, 2015
ABSTRACT Premise of research. Riparian plants are highly dependent on water sources; consequently... more ABSTRACT Premise of research. Riparian plants are highly dependent on water sources; consequently, general climatic conditions are less important to these taxa relative to woodland and shrubland species. This leads to interesting research questions regarding riparian plant taxa. Research on phylogeography of Mediterranean riparian tree and shrub species is scarce. In this article, we investigated the plastidial genetic diversity in Celtis australis L. (hackberry) and Nerium oleander L. (oleander) throughout the Mediterranean Basin. Both species are distributed in gullies, rivers, and stream banks under warm temperate climates. Methodology. Eighteen cpSSR loci and three noncoding cpDNA regions (rps16, rpl32-trnL, and trnQ-5′-rps16) were examined to assess the levels and geographic distribution of sequence variation in 41 hackberry and 56 oleander populations. The rpl32-trnL intergenic region was used for molecular dating analysis. Pivotal results. The respective 2762- and 3134-bp noncoding cpDNA regions sequenced in C. australis and N. oleander, as well as the 22 cpSSR analyzed fragments, exhibited the absence of variability in natural populations throughout the Mediterranean Basin. In N. oleander, two regions (rps16, rpl32-trnL) exhibited variability in three positions, and four cpSSR microsatellite motifs were polymorphic. The polymorphisms were geographically structured, and three haplotypes were characterized, two from Saharan populations and one from Mediterranean populations. Phylogeny and molecular dating analyses resulted in a tree with high consistency values of posteriori probability (PP = 1) and bootstrap support (98%) from the ingroup (N. oleander). The relaxed molecular clock model applied to the calibration of the tree estimates that the diversification of these haplotypes occurred in a range of 7.2–1.2 Ma. Conclusions. The absence of plastid variability in both hackberry and oleander is explained by a low mutation rate and/or recent recolonization of the Mediterranean Basin. Low temperatures during the Last Glacial Maximum produced freezing water along riparian corridors, with the consequent drastic contraction or even disappearance of both species in the Mediterranean Basin.
Los contenidos y opiniones expresados en este libro son responsabilidad exclusiva de los autores ... more Los contenidos y opiniones expresados en este libro son responsabilidad exclusiva de los autores y no tienen que coincidir necesariamente con los de la
Botanical Journal of the Linnean Society, 2013
Understanding the extent and distribution of genetic diversity within a species is essential for ... more Understanding the extent and distribution of genetic diversity within a species is essential for the development of effective conservation strategies. The objective of this study was to assess genetic variation using amplified fragment length polymorphisms (AFLP) in two species of the tropical legume genus Stylosanthes Sw. Annual, S. humilis (2 n = 20) and perennial, S. viscosa (2n = 20) are found throughout tropical America, and are sympatric for much of their range of distribution. One hundred and eleven accessions, covering a wide geographical range, were selected for AFLP analysis. Binary data matrices derived from DNA banding patterns were analysed using the software programs NTSYS -PC and ARLEQUIN . Several accessions were found to be misidentified. Of the S. humilis accessions, the overall average similarity value was (0.72) slightly higher than the value obtained for S. viscosa (0.67). Cluster analysis and principal coordinate analysis grouped accessions from both species by geographical origin, with a few exceptions. Analysis of molecular variance ( AMOVA ) in S. humilis revealed 59.4% of the variation among groups formed from the cluster analysis. This was highly significant ( P < 0.001). For S. viscosa AMOVA also revealed more variation among than within groups (66.5%). This was also highly significant ( P < 0.001). The majority of accessions of both species conserved ex situ are of Brazilian and Venezuelan origin. This study has identified areas in Central America and Mexico for which novel genetic variation may be found and where conservation activities should be focused.
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Papers by Antoni Aguilella