This report presents a novel lab-on-a-paper (LoP)-based device coupled with a molybdenum disulfid... more This report presents a novel lab-on-a-paper (LoP)-based device coupled with a molybdenum disulfide nanosheet (MoS 2 NS)-modified electrochemical genosensor for detecting Salmonella-specific DNA. Conductive electrodes were grafted on a paper-based substrate employing a stencil printing technique, and MoS 2 NS was decorated on the working electrode. MoS 2 NS has strong affinity toward nucleo bases, which made it a best sensing interface for the immobilization of DNA. Morphological, optical, and structural characterizations were accomplished using X-ray diffraction (XRD), high-resolution transmission electron microscopy (HR-TEM), UV−vis spectroscopy (UV−vis), and Raman spectroscopy, repectively. The current studies of an electrochemical genosensor demonstrated a good linear detection range from 100−20 nM and a low limit of detection of 20 nM toward Salmonella DNA with R 2 = 0.991. The proposed LoP-based genosensor confirmed as a better sensing podium and an effectual immobilization matrix for DNA.
Norovirus is one of the leading causes of gastroenteritis, acute vomiting, intense diarrhoea, acu... more Norovirus is one of the leading causes of gastroenteritis, acute vomiting, intense diarrhoea, acute pain in the stomach, high fever, headaches, and body pain. Conventional methods of detection gave us very promising results but had disadvantages such as low sensitivity, cost ineffectiveness, reduced specificity and selectivity, etc. Therefore, biosensors can be a viable alternative device which can overcome all setbacks associated with the conventional method. An electrochemical sensor based on oxidized graphitic carbon nitride (Ox-g-C3N4) modified electrochemical paper-based analytical device (ePAD) was fabricated for the detection of norovirus DNA. The synthesized Ox-g-C3N4 nanosheets were characterized by field emission scanning electron microscopy (FESEM), X-ray Diffraction (XRD), UV-Vis spectroscopy and X-Ray Photoelectron Spectroscopy. The capture probe DNA (PDNA) modified electrodes were characterized by cyclic voltammetry (CV) and differential pulse voltammetry (DPV). These ...
Chlorpyrifos (chl) is an organophosphate pesticide extensively used in agriculture and highly tox... more Chlorpyrifos (chl) is an organophosphate pesticide extensively used in agriculture and highly toxic for human health. Fluorine doped tin-oxide (FTO) based electrochemical nanosensor was developed for chlorpyrifos detection with gold nanoparticles (AuNPs) and anti-chlorpyrifos antibodies (chl-Ab). AuNPs provides high electrical conductivity and specific resistivity, thus increases the sensitivity of immunoassay. High electrical conductivity of AuNPs reveals that it promotes the redox reaction for better cyclic voltammetry. Based on the intrinsic conductive properties of FTO-AuNPs complex, chl-Ab was immobilized onto AuNPs surface. Under optimized conditions, the proposed FTO based nanosensor exhibited high sensitivity and stable response for the detection of chlorpyrifos, ranging from 1fM to 1µM with limit of detection (LOD) up to 10fM. The FTO-AuNPs sensor was successfully employed for the detection of chlorpyrifos in standard as well in real samples up to 10nM for apple and cabbage...
This report presents a novel lab-on-a-paper (LoP)-based device coupled with a molybdenum disulfid... more This report presents a novel lab-on-a-paper (LoP)-based device coupled with a molybdenum disulfide nanosheet (MoS 2 NS)-modified electrochemical genosensor for detecting Salmonella-specific DNA. Conductive electrodes were grafted on a paper-based substrate employing a stencil printing technique, and MoS 2 NS was decorated on the working electrode. MoS 2 NS has strong affinity toward nucleo bases, which made it a best sensing interface for the immobilization of DNA. Morphological, optical, and structural characterizations were accomplished using X-ray diffraction (XRD), high-resolution transmission electron microscopy (HR-TEM), UV−vis spectroscopy (UV−vis), and Raman spectroscopy, repectively. The current studies of an electrochemical genosensor demonstrated a good linear detection range from 100−20 nM and a low limit of detection of 20 nM toward Salmonella DNA with R 2 = 0.991. The proposed LoP-based genosensor confirmed as a better sensing podium and an effectual immobilization matrix for DNA.
Norovirus is one of the leading causes of gastroenteritis, acute vomiting, intense diarrhoea, acu... more Norovirus is one of the leading causes of gastroenteritis, acute vomiting, intense diarrhoea, acute pain in the stomach, high fever, headaches, and body pain. Conventional methods of detection gave us very promising results but had disadvantages such as low sensitivity, cost ineffectiveness, reduced specificity and selectivity, etc. Therefore, biosensors can be a viable alternative device which can overcome all setbacks associated with the conventional method. An electrochemical sensor based on oxidized graphitic carbon nitride (Ox-g-C3N4) modified electrochemical paper-based analytical device (ePAD) was fabricated for the detection of norovirus DNA. The synthesized Ox-g-C3N4 nanosheets were characterized by field emission scanning electron microscopy (FESEM), X-ray Diffraction (XRD), UV-Vis spectroscopy and X-Ray Photoelectron Spectroscopy. The capture probe DNA (PDNA) modified electrodes were characterized by cyclic voltammetry (CV) and differential pulse voltammetry (DPV). These ...
Chlorpyrifos (chl) is an organophosphate pesticide extensively used in agriculture and highly tox... more Chlorpyrifos (chl) is an organophosphate pesticide extensively used in agriculture and highly toxic for human health. Fluorine doped tin-oxide (FTO) based electrochemical nanosensor was developed for chlorpyrifos detection with gold nanoparticles (AuNPs) and anti-chlorpyrifos antibodies (chl-Ab). AuNPs provides high electrical conductivity and specific resistivity, thus increases the sensitivity of immunoassay. High electrical conductivity of AuNPs reveals that it promotes the redox reaction for better cyclic voltammetry. Based on the intrinsic conductive properties of FTO-AuNPs complex, chl-Ab was immobilized onto AuNPs surface. Under optimized conditions, the proposed FTO based nanosensor exhibited high sensitivity and stable response for the detection of chlorpyrifos, ranging from 1fM to 1µM with limit of detection (LOD) up to 10fM. The FTO-AuNPs sensor was successfully employed for the detection of chlorpyrifos in standard as well in real samples up to 10nM for apple and cabbage...
Uploads
Papers by Annu Mishra