Papers by Gabriela Alvarado
Solution Structure of the KIX Domain of CBP Bound to the Transactivation Domain of CREB: A Model for Activator:Coactivator Interactions
Cell, 1997
Structural analyses of CREB-CBP transcriptional activator-coactivator complexes by NMR spectroscopy: implications for mapping the boundaries of structural domains 1 1 Edited by F. E. Cohen
Journal of Molecular Biology, 1999
Using the KDSM Methodology for Knowledge Discovery from a Labor Domain
The paper presents the knowledge discovery in serial measures (KDSM) methodology as an easy and o... more The paper presents the knowledge discovery in serial measures (KDSM) methodology as an easy and optimal way for analyzing repeated very short serial measures with a blocking factor. An application to labor the domain is described using KDSM. A novel knowledge about labor domain's behavior was obtained once KDSM was applied to this specific domain. KDSM is a hybrid methodology (statistic and artificial intelligence) that gives a possible solution to a knowledge problem, especially when seemingly there are no relevant attributes.
Efficient identification of phosphatidylserine-binding proteins by ORF phage display
Biochemical and Biophysical Research Communications, 2009
Identification of Hnrph3 as an autoantigen for acute anterior uveitis
Clinical Immunology, 2011
Efficient identification of tubby-binding proteins by an improved system of T7 phage display
Journal of Molecular Recognition, 2009
Mutation in the tubby gene causes adult-onset obesity, progressive retinal, and cochlear degenera... more Mutation in the tubby gene causes adult-onset obesity, progressive retinal, and cochlear degeneration with unknown mechanism. In contrast, mutations in tubby-like protein 1 (Tulp1), whose C-terminus is highly homologous to tubby, only lead to retinal degeneration. We speculate that their diverse N-terminus may define their distinct disease profile. To elucidate the binding partners of tubby, we used tubby N-terminus (tubby-N) as bait to identify unknown binding proteins with open-reading-frame (ORF) phage display. T7 phage display was engineered with three improvements: high-quality ORF phage display cDNA library, specific phage elution by protease cleavage, and dual phage display for sensitive high throughput screening. The new system is capable of identifying unknown bait-binding proteins in as fast as ∼4–7 days. While phage display with conventional cDNA libraries identifies high percentage of out-of-frame unnatural short peptides, all 28 tubby-N-binding clones identified by ORF phage display were ORFs. They encode 16 proteins, including 8 nuclear proteins. Fourteen proteins were analyzed by yeast two-hybrid assay and protein pull-down assay with ten of them independently verified. Comparative binding analyses revealed several proteins binding to both tubby and Tulp1 as well as one tubby-specific binding protein. These data suggest that tubby-N is capable of interacting with multiple nuclear and cytoplasmic protein binding partners. These results demonstrated that the newly-engineered ORF phage display is a powerful technology to identify unknown protein–protein interactions. Copyright © 2009 John Wiley & Sons, Ltd.
Amino acid change 335 E to K affects the sialic-acid-binding and neuraminidase activities of Urabe AM9 mumps virus hemagglutinin–neuraminidase glycoprotein
Microbes and Infection, 2007
Ni sus escritos, ni los informes de los médicos sobre él(8), nos dan suficiente noticia sobre la ... more Ni sus escritos, ni los informes de los médicos sobre él(8), nos dan suficiente noticia sobre la prehistoria del paciente y las circunstancias de su vida. Yo ni siquiera podría indicar su edad en la época en que enfermó(9), si bien la alta jerarquía que había alcanzado en la administración de justicia antes de la segunda enfermedad supone indudablemente cierto mínimo. Nos enteramos de que en la época de la «hipocondría» el doctor Schreber llevaba ya largo tiempo casado. Escribe: «Un agradecimiento casi más ferviente todavía sintió mi mujer, que en el profesor Flechsig honraba, ni más ni menos, a quien le había devuelto a su marido, y por esa razón tuvo durante años su retrato sobre su mesa de trabajo» (36). Y allí mismo: «Tras la curación de mi primera enfermedad, he convivido con mi esposa ocho años, asaz felices en general, ricos también en honores externos, y sólo de tiempo en tiempo turbados por la repetida frustración de la esperanza de concebir hijos».
El presente cuestionario, desarrollado por Jay Hall, se propone ayudarte a comprender mejor tus a... more El presente cuestionario, desarrollado por Jay Hall, se propone ayudarte a comprender mejor tus actitudes en las Relaciones Interpersonales, recuerda que no hay respuestas correctas o incorrectas. La mejor respuesta será aquella que, de una manera más cercana, indique tú manera de ser habitual en la búsqueda de unas buenas relaciones interpersonales.
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Papers by Gabriela Alvarado