The aim of the present work was to evaluate the interaction between double-stranded DNA (dsDNA) a... more The aim of the present work was to evaluate the interaction between double-stranded DNA (dsDNA) and liposomes by voltammetric methods. The experimental results were analyzed considering the initial studies regarding the oxidation mechanism of dsDNA purine bases by cyclic and differential pulse voltammetry at the glassy carbon electrode (GCE). The interaction between dsDNA and 1,2-Dimyristoyl-sn-glycero-3phosphocholine (DMPC) was studied in a suspension containing both dsDNA and DMPC liposomes, prepared in pH = 7.0, 0.1 M phosphate buffer and using different incubation time periods. The formation of dsDNA-liposome complex was put in evidence by the decrease of the dsDNA oxidation peaks, dependent upon the incubation time. This behavior was explained considering the electroactive centers of dsDNA, guanosine monophosphate and adenosine monophosphate residues, part of them hidden inside the dsDNA-liposome complex structure and thus being unable to reach the GC electrode and preventing their oxidation. The electrochemical results are relevant for a better physicochemical characterisation of the dsDNA and dsDNA-liposome complex, which can be important for the development of gene therapy vectors.
EGO is a parallel molecular dynamics program running on Transputers. We conducted a performance a... more EGO is a parallel molecular dynamics program running on Transputers. We conducted a performance analysis of the EGO program in order to determine whether it was effectively using the computational resources of Transputers. Our first concern was whether communication was overlapped with computation, so that the overheads due to communication not overlapped with computation were less. With the assistance of performance tools such as UPSHOT, and with instrumentation of the EGO program itself, we were able to determine that only 8% of the execution time of the EGO program was spent in non-overlapping communication. Our next concern was that the MFLOPS rating of the EGO program was 0.25 MFLOPS, while the Transputers have a sustained rating of 1.5 MFLOPS. We measured MFLOPS ratings of small blocks of OCCAM code and determined that they matched the performance of the EGO code.
Evaluation of the melliferous potential of the species belonging to the Lamiaceae family identified and studied in the bioapiculture area Danube valley
One of the major concerns of phytotherapy is enhancement of the new sources of raw materials rich... more One of the major concerns of phytotherapy is enhancement of the new sources of raw materials rich in active principles. Research in this field are considering as inexhaustible the sources and performances of plant materials provided by nature. The subject of this study fits the current trend of development of the biotechnologies that have an impact on maintaining and improving the quality of life. This represents a topic of general interest through the subject approached, namely: research regarding the protective role of flavone compounds to prevent and "stop" the lipids oxidation due to free radical attack. The aim of this research was to evaluate, by comparison, the antioxidant potential of two extracts, obtained from Ribes Nigrum and Vaccinium Myrtillus leaves, on lipid oxidation from artificial membranes.
The allelopathy research can contribute to protecting the biodiversity and may develop new strate... more The allelopathy research can contribute to protecting the biodiversity and may develop new strategies for sustainable ecosystems controlled by allelochemicals. The present investigations have estimated the allelopahtic features at Festuca rubra and the ability of the allelopathic compounds to affect the germination and the quality of perennial grasses (Dactyilis glomerata, Lolium perenne and Poa pratensis). These perennial grasses were treated with alcolic extracts obtained from dry aerial parts of Festuca Rubra. Were quantified and also physico–chemical characterized the polyphenolic compounds and the alkaloids from the chemical structure of plants. The extracted compounds from the dry aerialparts of F. Rubra were quantified using HPLC method. In the aqueous extracts were quantified the following alkaloids: N–formyl–loline (NFL), N–acetyl–loline (NAL) and ergovaline (EGV). The results of the research have showed that the effect of the alkaloids lead to modifications in the quality ...
Antimicrobial peptides have important roles in the innate immune defenses. Here we studied the dy... more Antimicrobial peptides have important roles in the innate immune defenses. Here we studied the dynamics and structural properties of the antimicrobial peptide 2 from Amaranthus caudatus by molecular dynamics simulation.
The present study pursued the possibility of extraction of sugars and other useful components fro... more The present study pursued the possibility of extraction of sugars and other useful components from fresh marc, by diffusion method. The biological material was represented by two types of marc: one from Greaca Vineyard-Wine Research and Production Station and one from Vinalcool Focsani. It was studied the influence of temperature, time, pH and the amount of washing solution on the diffusion of sugars and other useful components. Extraction efficiency was monitored by determining total sugars in the diffusion solutions, and by measuring the volume of solution. Besides the main components which can be found in large quantities: sugars, nitrogen and potassium tartrate, the fresh marc contains, in various concentrations, phosphorus, calcium and magnesium, also. These ingredients are partially passing into the diffusion solution, some remaining in the washed marc. For this reason, the marc continues to remain, even after diffusion, a raw material that can be further used for valorification.
The aim of the present work was to evaluate the interaction between double-stranded DNA (dsDNA) a... more The aim of the present work was to evaluate the interaction between double-stranded DNA (dsDNA) and liposomes by voltammetric methods. The experimental results were analyzed considering the initial studies regarding the oxidation mechanism of dsDNA purine bases by cyclic and differential pulse voltammetry at the glassy carbon electrode (GCE). The interaction between dsDNA and 1,2-Dimyristoyl-sn-glycero-3phosphocholine (DMPC) was studied in a suspension containing both dsDNA and DMPC liposomes, prepared in pH = 7.0, 0.1 M phosphate buffer and using different incubation time periods. The formation of dsDNA-liposome complex was put in evidence by the decrease of the dsDNA oxidation peaks, dependent upon the incubation time. This behavior was explained considering the electroactive centers of dsDNA, guanosine monophosphate and adenosine monophosphate residues, part of them hidden inside the dsDNA-liposome complex structure and thus being unable to reach the GC electrode and preventing their oxidation. The electrochemical results are relevant for a better physicochemical characterisation of the dsDNA and dsDNA-liposome complex, which can be important for the development of gene therapy vectors.
EGO is a parallel molecular dynamics program running on Transputers. We conducted a performance a... more EGO is a parallel molecular dynamics program running on Transputers. We conducted a performance analysis of the EGO program in order to determine whether it was effectively using the computational resources of Transputers. Our first concern was whether communication was overlapped with computation, so that the overheads due to communication not overlapped with computation were less. With the assistance of performance tools such as UPSHOT, and with instrumentation of the EGO program itself, we were able to determine that only 8% of the execution time of the EGO program was spent in non-overlapping communication. Our next concern was that the MFLOPS rating of the EGO program was 0.25 MFLOPS, while the Transputers have a sustained rating of 1.5 MFLOPS. We measured MFLOPS ratings of small blocks of OCCAM code and determined that they matched the performance of the EGO code.
Evaluation of the melliferous potential of the species belonging to the Lamiaceae family identified and studied in the bioapiculture area Danube valley
One of the major concerns of phytotherapy is enhancement of the new sources of raw materials rich... more One of the major concerns of phytotherapy is enhancement of the new sources of raw materials rich in active principles. Research in this field are considering as inexhaustible the sources and performances of plant materials provided by nature. The subject of this study fits the current trend of development of the biotechnologies that have an impact on maintaining and improving the quality of life. This represents a topic of general interest through the subject approached, namely: research regarding the protective role of flavone compounds to prevent and "stop" the lipids oxidation due to free radical attack. The aim of this research was to evaluate, by comparison, the antioxidant potential of two extracts, obtained from Ribes Nigrum and Vaccinium Myrtillus leaves, on lipid oxidation from artificial membranes.
The allelopathy research can contribute to protecting the biodiversity and may develop new strate... more The allelopathy research can contribute to protecting the biodiversity and may develop new strategies for sustainable ecosystems controlled by allelochemicals. The present investigations have estimated the allelopahtic features at Festuca rubra and the ability of the allelopathic compounds to affect the germination and the quality of perennial grasses (Dactyilis glomerata, Lolium perenne and Poa pratensis). These perennial grasses were treated with alcolic extracts obtained from dry aerial parts of Festuca Rubra. Were quantified and also physico–chemical characterized the polyphenolic compounds and the alkaloids from the chemical structure of plants. The extracted compounds from the dry aerialparts of F. Rubra were quantified using HPLC method. In the aqueous extracts were quantified the following alkaloids: N–formyl–loline (NFL), N–acetyl–loline (NAL) and ergovaline (EGV). The results of the research have showed that the effect of the alkaloids lead to modifications in the quality ...
Antimicrobial peptides have important roles in the innate immune defenses. Here we studied the dy... more Antimicrobial peptides have important roles in the innate immune defenses. Here we studied the dynamics and structural properties of the antimicrobial peptide 2 from Amaranthus caudatus by molecular dynamics simulation.
The present study pursued the possibility of extraction of sugars and other useful components fro... more The present study pursued the possibility of extraction of sugars and other useful components from fresh marc, by diffusion method. The biological material was represented by two types of marc: one from Greaca Vineyard-Wine Research and Production Station and one from Vinalcool Focsani. It was studied the influence of temperature, time, pH and the amount of washing solution on the diffusion of sugars and other useful components. Extraction efficiency was monitored by determining total sugars in the diffusion solutions, and by measuring the volume of solution. Besides the main components which can be found in large quantities: sugars, nitrogen and potassium tartrate, the fresh marc contains, in various concentrations, phosphorus, calcium and magnesium, also. These ingredients are partially passing into the diffusion solution, some remaining in the washed marc. For this reason, the marc continues to remain, even after diffusion, a raw material that can be further used for valorification.
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Papers by Alina Butu