Papers by Alexander Seifalian
Recent Patents on Biotechnology, Dec 21, 2018
He organized several international and national meetings and symposia (IBRO meeting, Glia confere... more He organized several international and national meetings and symposia (IBRO meeting, Glia conferences etc.). His projects were funded by the EU, DFG, DAAD,national foundations and several industrial companies. He received several awards among them the MerckleForschungspreis 2001, the price of the Society of Steroid Biochemistry in 2002, and the RWTH Teaching Award in 2007. He is a member of the Editorial board of several peer-review journals such as the Annals of Anatomy and Current Neuropharmacology, and published several special issues in the Journal of Biochemistry and Molecular Biology. To this day, he published more than 190 peer reviewed articles with more than 3500 citations.
British Journal of Surgery, Mar 12, 2009
Sir I read with interest the review by Seyednejad et al. on topical haemostatic agents. The autho... more Sir I read with interest the review by Seyednejad et al. on topical haemostatic agents. The authors have made a tremendous effort to give an overview of all available clotting agents and their advantages and disadvantages. Nevertheless, I would like to point out an error in figure 5 of the review. This figure depicts the relationship between different topical agents and components of the clotting cascade such as the intrinsic and extrinsic pathways. More than 5 years ago, the conceptual thoughts about the functional components of the coagulation pathway changed. In the sixties, the cascade and waterfall hypotheses proposed an intrinsic pathway of coagulation initiated by contact factors. The extrinsic tissue factor pathway was thought to play an ancillary role in activation of coagulation. The segregation of the coagulation system into the intrinsic and extrinsic pathways fails to reflect coagulation accurately. The current view is that coagulation predominantly proceeds by the tissue factor/factor-VIIa pathway. Thrombin is generated after exposure of F VIIa to TF and subsequent activation of factor X into Xa. Both TF/F-VIIa complex and F Xa are inhibited by tissue factor pathway inhibitor. Interaction of the cofactor molecule factor V with factor Xa results in increased thrombin generation. The TF/F-VIIa pathway has several amplification loops, which maintain Xa generation via positive feedback mechanisms involving factors VIII, IX and XI. Thrombin generation is downregulated by antithrombin and activated protein C. Thus, currently a more holistic view is held on activation of coagulation and its amplification and inhibition loops than two pathways that both separately end in thrombin formation. M. A. Boermeester Department of Surgery, Academic Medical Center, Meibergdreef 9, 1105AZ Amsterdam, The Netherlands DOI: 10.1002/bjs.6602 Authors’ reply: Topical haemostatic agents (Br J Surg 2008; 95: 1197–1225)
HAL (Le Centre pour la Communication Scientifique Directe), 2021
Proceedings of SPIE, Jun 27, 2003
Haemostatic effects of laser tissue solder as a reinforcement to anastomoses with PTFE grafts. [P... more Haemostatic effects of laser tissue solder as a reinforcement to anastomoses with PTFE grafts. [Proceedings of SPIE 4949, 235 (2003)]. Jeremy F. Birch, Alexander M. Seifalian, Peter R. Bell. Abstract. Laser activated tissue solders ...
Scanning, Jan 18, 2022
The heavy casualties associated with mass disasters necessitate substantial resources to be manag... more The heavy casualties associated with mass disasters necessitate substantial resources to be managed. The unexpectedly violent nature of such occurrences usually remains a problematic amount of victims that urgently require to be identified by a reliable and economical method. Conventional identification methods are inefficient in many cases such as plane crashes and fire accidents that have damaged the macrobiometric features such as fingerprints or faces. An appropriate recognition method for such cases should use features more resistant to destruction. Forensic dentistry provides the most appropriate available method for the successful identification of victims using careful techniques and precise data interpretation. Since bones and teeth are the most persistent parts of the demolished bodies in sudden mass disasters, scanning and radiographs are unrepeatable parts of forensic dentistry. Forensic dentistry as a scientific method of human remain identification has been considerably referred to be efficient in disasters. Forensic dentistry can be used for either "sex and age estimation," "Medical biotechnology techniques," or "identification with dental records," etc. The present review is aimed at discussing the development and implementation of forensic dentistry methods for human identification. For this object, the literature from the last decade has been searched for the innovations in forensic dentistry for human identification based on the PubMed database.
BioMed Research International, Apr 6, 2022
Multimicrobial infections caused by pathobionts are called dysbiotic multimicrobial illnesses. Co... more Multimicrobial infections caused by pathobionts are called dysbiotic multimicrobial illnesses. Commercial mouthwashes, such as chlorhexidine, have negative side effects that can prevent tooth decay and infection. The present study aimed to determine the antifungal, antibacterial, and cytotoxicity characteristics of the propolis extracts from different areas (Iran). The ethanolic extract of propolis was prepared. GC/MS carried out the characterization to determine the thymol, carvacrol, and menthol extracts, and also, total phenol and flavonoid were assed for all samples. The antimicrobial and antibiofilm effects were evaluated against S. mutans, S. mitis, S. salivarius, L. acidophilus, E. coli, S. aureus, and C. albicans. The cytotoxic effect of extracts was measured on human fibroblast cells by MTT test. The MIC values in mg mL-1 were ranged as follows: S. salivarius (0.003 to 0.048), S. mutans (0.003 to 0.029), S. mitis (0.007 to 0.058), L. acidophilus (0.007 to 0.117), C. albicans (0.014 to 0.234), E. coli (0.007 to 0.058), and S. aureus (0.007 to 0.058), while MBC were, respectively, S. mutans (0.007 to 0.058), S. salivarius (0.007 to 0.117), S. mitis (0.007 to 0.117), L. acidophilus (0.014 to 0.234), C. albicans (0.029 to 0.468), E. coli (0.014 to 0.234), and S. aureus (0.007 to 0.117). Cariogenic bacteria and Candida albicans were demonstrated to be resistant to propolis extracts. Therefore, propolis extracts may make good mouthwashes.
Excli Journal, Feb 22, 2021
Recently, a growing attention has been observed toward potential advantages of stem cell (SC)-bas... more Recently, a growing attention has been observed toward potential advantages of stem cell (SC)-based therapies in regenerative treatments. Mesenchymal stem/stromal cells (MSCs) are now considered excellent candidates for tissue replacement therapies and tissue engineering. Autologous MSCs importantly contribute to the state-of-theart clinical strategies for SC-based alveolar bone regeneration. The donor cells and immune cells play a prominent role in determining the clinical success of MSCs therapy. In line with the promising future that stem cell therapy has shown for tissue engineering applications, dental stem cells have also attracted the attention of the relevant researchers in recent years. The current literature review aims to survey the variety and extension of SC-application in tissue-regenerative dentistry. In this regard, the relevant English written literature was searched using keywords: "tissue engineering", "stem cells", "dental stem cells", and "dentistry strategies". According to the available database, SCs application has become increasingly widespread because of its accessibility, plasticity, and high proliferative ability. Among the growing recognized niches and tissues containing higher SCs, dental tissues are evidenced to be rich sources of MSCs. According to the literature, dental SCs are mostly present in the dental pulp, periodontal ligament, and dental follicle tissues. In this regard, the present review has described the recent findings on the potential of dental stem cells to be used in tissue regeneration.
Evidence-based Complementary and Alternative Medicine, Aug 21, 2021
Dental plaque is a biofilm composed of complex microbial communities. It is the main cause of maj... more Dental plaque is a biofilm composed of complex microbial communities. It is the main cause of major dental diseases such as caries and periodontal diseases. In a healthy state, there is a delicate balance between the dental biofilm and host tissues. Nevertheless, due to the oral cavity changes, this biofilm can become pathogenic. e pathogenic biofilm shifts the balance from demineralization-remineralization to demineralization and results in dental caries. Dentists should consider caries as a result of biological processes of dental plaque and seek treatments for the etiologic factors, not merely look for the treatment of the outcome caused by biofilm, i.e., dental caries. Caries prevention strategies can be classified into three groups based on the role and responsibility of the individuals doing them: (1) community-based strategy, (2) dental professionals-based strategy, and (3) individual-based strategy. e community-based methods include fluoridation of water, salt, and milk. e dental professionals-based methods include professional tooth cleaning and use of varnish, fluoride gel and foam, fissure sealant, and antimicrobial agents. e individualbased (self-care) methods include the use of fluoride toothpaste, fluoride supplements, fluoride mouthwashes, fluoride gels, chlorhexidine gels and mouthwashes, slow-release fluoride devices, oral hygiene, diet control, and noncariogenic sweeteners such as xylitol. is study aimed to study the research in the recent five years (2015-2020) to identify the characteristics of dental biofilm and its role in dental caries and explore the employed approaches to prevent the related infections.
Life Sciences, May 1, 2020
This is a PDF file of an article that has undergone enhancements after acceptance, such as the ad... more This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.
Future Cardiology, Mar 1, 2011
Percutaneous transluminal coronary angioplasty revolutionized therapy for coronary artery disease... more Percutaneous transluminal coronary angioplasty revolutionized therapy for coronary artery disease. This early promise of a viable alternative to surgical treatment of coronary artery disease was thwarted by the high rates of angiographic restenosis. The advent of stenting reduced the rates of restenosis, although it was hindered by the new problem of in-stent restenosis. It was demonstrated that in-stent restenosis was the result of a new pathology in the form of neointimal hyperplasia, which was a maladaptive healing response to bare-metal stent implantation. Recently, the introduction of drug-eluting stents (DES) technology has offered a new solution to the problem of restenosis. Current evidence suggests that although DES have reduced restenosis rates, important concerns have been raised regarding increased stent thrombosis, myocardial infarction and death. The purpose of this article is to examine the efficacy and safety data of DES as highlighted in recent publications and to further discuss the biomolecular mechanisms of accelerated endothelization and stent thrombosis. In addition, we will examine some of the newer stent technologies available.
Bioinorganic Chemistry and Applications, Mar 3, 2022
Orodental problems have long been managed using herbal medicine. e development of nanoparticle fo... more Orodental problems have long been managed using herbal medicine. e development of nanoparticle formulations with herbal medicine has now become a breakthrough in dentistry because the synthesis of biogenic metal nanoparticles (MNPs) using plant extracts can address the drawbacks of herbal treatments. Green production of MNPs such as Ag, Au, and Fe nanoparticles enhanced by plant extracts has been proven to be beneficial in managing numerous orodental disorders, even outperforming traditional materials. Nanostructures are utilized in dental advances and diagnostics. Oral disease prevention medicines, prostheses, and tooth implantation all employ nanoparticles. Nanomaterials can also deliver oral fluid or pharmaceuticals, treating oral cancers and providing a high level of oral healthcare. ese are also found in toothpaste, mouthwash, and other dental care products. However, there is a lack of understanding about the safety of nanomaterials, necessitating additional study. Many problems, including medication resistance, might be addressed using nanoparticles produced by green synthesis. is study reviews the green synthesis of MNPs applied in dentistry in recent studies (2010-2021).
European Journal of Cardio-Thoracic Surgery, Aug 18, 2020
OBJECTIVES: The postoperative persistence of chylothorax is a fatal complication of paediatric ca... more OBJECTIVES: The postoperative persistence of chylothorax is a fatal complication of paediatric cardiac surgery. There is an urgent need for an effective treatment of chylothorax. This study reports the application of allogenic platelet-rich plasma fibrin glue (PRP-FG) as a conservative therapy before reoperation. METHODS: Over a 9-year period, from 2010 to 2019, 27 patients with persistent chylothorax following a cavopulmonary connection, with a mean latency period of 11 days (range 10-15 days), were treated with PRP-FG. These patients were selected because they had not responded positively to initial conservative management plans. The patients were followed up for 9 years. RESULTS: Twenty-five patients (92%) responded positively to treatment with PRP-FG; 2 patients did not respond to the treatment and died after reoperation. All of the successfully treated patients in follow-up continued to live a healthy life without further complications.
AMB Express, Jun 15, 2022
Dental caries and oral infections have become a widespread issue in the modern world. This study ... more Dental caries and oral infections have become a widespread issue in the modern world. This study aimed to investigate the antibacterial, antifungal, and cytotoxicity characteristics of the extracts of Echinacea purpura, Arctium lappa, and the essential oil of Zataria multiflora as a potential herbal mouthwash. The essential oil of Z. multiflora leaves and the extracts of E. purpurea and A. lappa roots were prepared. The characterization was carried out by GC-MS and also, total phenol and flavonoid were assed for all three samples. The antimicrobial and anti-biofilm effects were evaluated against Streptococcus mutans, Streptococcus mitis, Streptococcus salivarius, Lactobacillus acidophilus, Escherichia coli, Staphylococcus aureus, and Candida albicans. The cytotoxic effect of the samples was evaluated on HEK 293 and HDFa cells by MTT test. Thymol and carvacrol contents in EO of Z. multiflora were measured at 31% and 42.2%, respectively. A. lappa had the lowest total phenolic and flavonoid value among the samples. On the other hand, the total phenolic content of Z. multiflora and the total flavonoid content of E. purpurea were the highest. The MIC values of Zataria, Arctium, and Echinacea against S. mutans were 0.011% v/v, 187.5 mg/ml, and 93.75 mg/ml, while MBC were 0.011% v/v, 375 mg/ml, and 187.5 mg/ml, respectively. The formulation showed bactericidal activity against S. mutans in the concentration of 5.86 mg/ml for Echinacea and Burdock extracts and 0.08 µl/ml for EO of Zataria. The formulation significantly affected microbial biofilm formation and induced biofilm degradation. The cell viability percentages were higher than 50% during 24 and 48 h. The formulation had a significant antimicrobial effect on cariogenic bacteria and C. albicans, with the lowest cytotoxic effects. Therefore, this formulation can be an appropriate candidate for mouthwash.
European Journal of Pharmacology, Dec 1, 2020
Since the time that human population comprehended the importance of general health maintenance an... more Since the time that human population comprehended the importance of general health maintenance and the burden of disease, there has been a search for healing properties in the natural environment. Herbal medicine is the use of plants with medical properties for prevention and treatment of conditions that can affect general health. Recently, a growing interest has been observed toward the use of traditional herbal medicine alongside synthetic modern drugs. Around 80% of the population, especially in developing countries relies on it for healthcare. Oral healthcare is considered a major part of general health. According to the world health organization (WHO), oral health is considered an important part of general health and quality of life. The utilization of natural medications for the management of pathologic oro-dental conditions can be a logical alternative to pharmaceutical methods due to their availability, low costs, and lower side effects. The current literature review aimed at exploration of the variety and extent of herbal products application in oral health maintenance including different fields of oral healthcare such as dental caries, periodontal maintenance, microbial infections, oral cancers, and inflammatory conditions.
The Breast, Aug 1, 1998
Highly vascularized tumours are associated with fast growth and high grade. Accurate, objective a... more Highly vascularized tumours are associated with fast growth and high grade. Accurate, objective assessment of skin blood flow in the breast can be obtained by the recent development of a laser Doppler imager (LDI) that creates an image of tissue blood flow over a wide area. The imager collects flow data without touching the breast skin. Breast skin blood flow was mapped using the LDI in an outpatient clinic on 100 patients (47 normal, 26 benign breast disease and 27 breast carcinoma). The findings were correlated with the results of examination by mammography/ultrasound and cytology/histology. The LDI blood flow images of the normal breasts were analysed in five breast regions (four quadrants and central area) and flow values for each region were averaged. The mean blood flow in normal patients was 299 2 155 and 311 t-157 flux (k SD) for the right and left breasts, respectively. In patients with benign breast disease it was 476 f 210 flux and in patients with breast carcinoma it was 685 * 278 flux. Breasts with carcinoma show the highest skin blood flow measurements. Our findings indicate that the technique is sensitive for large cancers. This new tool may have an application in the serial monitoring of early response to systemic treatment in patients with large superficial tumour masses.
Journal of materials research and technology, Sep 1, 2020
Reconstructive orthodontics is increasingly benefiting from regenerative medicine and tissue engi... more Reconstructive orthodontics is increasingly benefiting from regenerative medicine and tissue engineering strategies. The scaffolds used both in reconstruction or in the regeneration of dental/oral defects are mainly are from two sources of materials: biologic or organic. The biologic materials have been used for regeneration and functional reconstruction of defected or lost tissues. The 3D scaffold from organic materials, including; collagen and hyaluronic acid derivatives with macromolecular properties similar to natural ECM. Bone and dentine regeneration is a growing field of research in dentistry and envisions clinical promises by improving the outcomes for the treatment of craniofacial repair and replacement. This has been recently accelerated with innovative materials and bioactive molecules and growth factors using in vitro or in vivo bioreactors. Both scaffolds made from biologic and organic materials have been combined with adult stem cells with mesenchymal phenotype in a biomimicry approach to creating the more natural-like bone substitutes. This research critically reviewed the literature in the past ten years from databases (PubMed and NCBI).
Stem cell reviews and reports, May 4, 2022
Journal of Biomedical Nanotechnology, Sep 1, 2015
Tendon injuries are a common and rising occurrence, associated with significant impairment to qua... more Tendon injuries are a common and rising occurrence, associated with significant impairment to quality of life and financial burden to the healthcare system. Clinically, they represent an unresolved problem, due to poor natural tendon healing and the inability of current treatment strategies to restore the tendon to its native state. Tissue engineering offers a promising alternative, with the incorporation of scaffolds, cells and growth factors to support the complete regeneration of the tendon. The materials used in tendon engineering to date have provided significant advances in structural integrity and biological compatibility and in many cases the results obtained are superior to those observed in natural healing. However, grafts fail to reproduce the qualities of the pre-injured tendon and each has weaknesses subject to its constituent parts. Furthermore, many materials and cell types are being investigated concurrently, with seemingly little association or comparison between research results. In this review the properties of the most-investigated and effective components have been appraised in light of the surrounding literature, with research from early in-vitro experiments to clinical trials being discussed. Extensive comparisons have been made between scaffolds, cell types and growth factors used, listing strengths and weaknesses to provide a stable platform for future research. Promising future endeavours are also described in the field of nanocomposite material science, stem cell sources and growth factors, which may bypass weaknesses found in individual elements. The future of tendon engineering looks bright, with growing understanding in material technology, cell and growth factor application and encouraging recent advances bringing us ever closer to regenerating the native tendon.
Journal of materials research and technology, Jun 1, 2021
This is a PDF file of an article that has undergone enhancements after acceptance, such as the ad... more This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.
Nanomedicine
Aim: Despite scientific advances, the number of cardiovascular patients is increasing worldwide. ... more Aim: Despite scientific advances, the number of cardiovascular patients is increasing worldwide. To protect damaged cardiomyocytes from further harm, novel and safer approaches are needed to help regeneration and prevent fibrosis. Methods: In this study, we performed a systematic review of in vitro and preclinical studies of carbon nanotubes (CNTs) and carbon nanofibers (CNFs) for help to treat heart damage. Conclusion: CNTs/CNFs in hydrogels cause higher conductivity, and the in case of alignment this increase is more than the random state. CNTs/CNFs can improve structural specification of the hydrogel for cardiac cell proliferation and enhance expression of genes associated with final differentiation of various stem cells to cardiac cells.
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Papers by Alexander Seifalian