Papers by Alejandro Montañez
... María-Elena López-Ortega,* Ernesto Santiago-Luna,* Mario Salazar-Páramo,** José Luis Montañez... more ... María-Elena López-Ortega,* Ernesto Santiago-Luna,* Mario Salazar-Páramo,** José Luis Montañez-Fernández,*** Jacqueline Osuna-Rubio,** Alejandro González ... Vesely DL, Preston R, Gower WR Jr, Chiou S, Epstein M. Increased release of kaliuretic peptide during immersion ...
European Journal of Cancer, 2001
The aim of this study was to determine the maximum-tolerated dose (MTD) and dose-limiting toxicit... more The aim of this study was to determine the maximum-tolerated dose (MTD) and dose-limiting toxicity (DLT) of weekly Irinotecan (CPT-11) plus UFT, and to assess the antitumour activity of this combination as second-line chemotherapy in patients with advanced colorectal carcinoma, 31 patients with measurable advanced colorectal carcinoma were treated. Cohorts of 3 patients received increasing dose levels of the combination. Levels 1 to 4 included a fixed dose of oral (p.o.) UFT (250 mg/m 2 /day) for 21 days of a 28-day cycle combined with increasing intravenous (i.v.) doses of 100, 110 and 120 mg/m 2 ) on days 1, 8 and 15. Levels 5 and 6 included a higher fixed dose of oral UFT (300 mg/m 2 ) combined with increasing i.v. doses of CPT-11 (100 and 110 mg/m 2 ) on days 1, 8 and 15. 147 courses were administered. MTD were reached at level 4 (2 cases of grade 4 diarrhoea and 1 grade 3 asthenia), and level 6 (1 grade 4 diarrhoea, 1 grade 3 diarrhoea and 1 grade 3 febrile neutropenia). Responses in 30 evaluable patients were: 3 partial responses (10%), 15 stable disease (50%) and progressive disease in 12 patients (40%). Median time to progression was 4.5 months (95% Confidence Interval (CI): 3.4-6.6 months) and median survival was 11 months (95% CI: 7.9-14.1 months). The recommended doses for phase II trials are: (a) CPT-11 110 mg/m 2 i.v. on days 1, 8 and 15 every 28 days plus UFT 250 mg/m 2 p.o. on days 1 through to 21 or (b) CPT-11 100 mg/m 2 and UFT 300 mg/m 2 . #
Arthroscopy-the Journal of Arthroscopic and Related Surgery, 2000
Isolated posterior cruciate ligament injuries are rare and their treatment is controversial. Thes... more Isolated posterior cruciate ligament injuries are rare and their treatment is controversial. These lesions have commonly been treated by open reduction and internal fixation using a posterior approach. However, this approach makes it difficult to explore other combined injuries of the knee joint. We report 2 cases of posterior cruciate ligament avulsion of the tibia that were arthroscopically reduced and fixed using 2 different methods, cannulated screws and tension band wire.
Brazilian Archives of Biology and Technology, 2000
Pectinesterase was extracted from potato alpha cultivar, purified and partially characterized The... more Pectinesterase was extracted from potato alpha cultivar, purified and partially characterized The used protocol resulted in a 58.8-fold purification (51 850.2 units/mg protein) with 15.5% recovery of pectinesterase activity. The purified enzyme had a molecular weight of 27 kDa and its isoelectric point was around 4.5 with pH and temperature optima of 8.0 and 60°C, respectively. The purified enzyme had a single symmetric peak of specific activity after chromatographic steps. The homogeneity of the purified pectinesterase was confirmed by gel filtration and polyacrylamide electrophoresis gel.
Arthroscopy-the Journal of Arthroscopic and Related Surgery, 2006
Purpose: To compare the initial mechanical characteristics of 4 systems used to fix tendons to th... more Purpose: To compare the initial mechanical characteristics of 4 systems used to fix tendons to the femur during anterior cruciate ligament reconstruction. Methods: A total of 32 porcine femurs were used to study the following fixation systems: Bioabsorbable interference screw (Stryker, Kalamazoo, MI), Bio-Transfix Cross-pin (Arthrex, Naples, FL), Biosteon Cross-pin (Stryker), and a fixation technique based on wrapping the graft around the femoral condyle itself, thus allowing it to be fixed in place without the use of any hardware. The mechanical characteristics of each system were obtained by a preconditioned failure tensile test. Results: The yield load values (990.9 ± 242.6 N for Bio-Transfix, 905.1 ± 158.8 N for Biosteon Cross-pin, 684.4 ± 119.7 N for the without-hardware system (WHS), and 369.4 ± 120.1 N for the interference screw) revealed significant differences between the techniques that used cross-pins and the other 2 techniques (P < .006) on the one hand, and between the without hardware technique and the interference screw (P < .004) on the other. The stiffness of the 2 cross-pin fixation systems (117.6 ± 22.5 N for Bio-Transfix and 112.6 ± 22.5 N for Biosteon) was greater (P < .01) than those of the other systems (79.4 ± 15.2 N for the WHS and 68.5 ± 13 N for the interference screw). Conclusions: The initial biomechanical properties of the 2 cross-pin fixation systems proved to be superior to those of the other 2 systems studied. The WHS fixation system exhibited better mechanical properties than its interference screw counterpart. Clinical Relevance: The better initial mechanical characteristics encountered using the Bio-Transfix and Biosteon Cross-pin systems indicate that these systems are better equipped to bear the loads generated by aggressive rehabilitation. The WHS fixation system provides an alternative to interference screw fixation.
Salud Publica De Mexico, 2009
salud pública de méxico / vol. 51, no. 4, julio-agosto de 2009 Lazcano-Ponce E y col.
Microbiological Research
Ustilago maydis displays dimorphic growth, alternating between a saprophytic haploid yeast form a... more Ustilago maydis displays dimorphic growth, alternating between a saprophytic haploid yeast form and a filamentous dikaryon, generated by mating of haploid cells and which is an obligate parasite. Induction of the dimorphic transition of haploid strains in vitro by change in ambient pH has been used to understand the mechanisms governing this differentiation process. In this study we used suppression subtractive hybridization to generate a cDNA library of U. maydis genes up-regulated in the filamentous form induced in vitro at acid pH. Expression analysis using quantitative RT-PCR showed that the induction of two unigenes identified in this library coincided with the establishment of filamentous growth in the acid pH medium. This expression pattern suggested that they were specifically associated to hyphal development rather than merely acid pH-induced genes. One of these genes, UmRrm75, encodes a protein containing three RNA recognition motifs and glycine-rich repeats and was selected for further study. The UmRrm75 gene contains 4 introns, and produces a splicing variant by a 3 -alternative splicing site within the third exon. Mutants deleted for UmRrm75 showed a slower growth rate than wild type strains in liquid and solid media, and their colonies showed a donut-like morphology on solid medium. Interestingly, although UmRrm75 strains were not affected in filamentous growth induced by acid pH and oleic acid, they exhibited reduced mating, post-mating filamentous growth and virulence. Our data suggest that UmRrm75 is probably involved in cell growth, morphogenesis, and pathogenicity in U. maydis.
Scientia Marina, 2010
ricker's stock-recruitment equation, including the multivariate enso index (Mei) as an environmen... more ricker's stock-recruitment equation, including the multivariate enso index (Mei) as an environmental variable, was fitted to data of the Pacific sardine (Sardinops sagax caerulea) fishery from Magdalena Bay, Baja California sur, Mexico. the equation was then incorporated into an age-structured, bioeconomic model. uncertainty was incorporated by using environmental fluctuations; seven-year projections, equivalent to one sardine generation, were computed. Five management options were considered in the projections: open access, effort at maximum sustainable economic yield (f Mse ), catch at maximum sustainable yield (C MsY ), equivalent effort level applied in the year 2004 (f 2004 ), and an arbitrary catch quota of 40000 tons (CQ 40000 ). Projection results were summarised by the indicator net Present Value (nPV) of the fishery for the modelled period. the strategies that involved some regulation in the effort level (f Mse and f 2004 ) yielded the largest nPV. on the other hand, the strategies that involved regulation of the catch predicted-in addition to a lower nPV-a substantial increase in the effort to achieve the desired catch level. this study is the first bioeconomic approach for a sardine fishery management plan in this region.
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Papers by Alejandro Montañez