Fountain Journal of National ad Applied Sciences, Dec 28, 2022
Plant products provide a vast source of therapeutics, but not without toxicity. This study evalua... more Plant products provide a vast source of therapeutics, but not without toxicity. This study evaluated the in vitro cytotoxic and proliferative activity of selected plant extracts on human peripheral blood mononuclear cells (PBMCs). The PBMCs from healthy donors were exposed to varying concentrations (25 µg/ml, 50 µg/ml, 100 µg/ml and 200 µg/ml) of aqueous extracts of young leaves of Mangifera indica (MI) and stem barks of Commiphora kerstingii (CK), and Lannea acida and Acacia sieberiana formulation (LAASF). Trypan blue assay was used to determine the viability of human PBMCs after isolation. Cytotoxicity and proliferation of PBMCs were determined using WST-8 assay. The total viable cell count of the isolated PBMCs in this study was 8000 x 10 4 cells/ml while viability was 96.15%. The extract of LAASF showed the lowest percentage cytotoxicity (2.63%) at 25 µg/ml concentration, followed by CK (2.70%), then MI (7.71%). There was significant decrease in PBMCs mean absorbance scores across the different concentrations of MI (p=0.008), CK (p<0.0001) and LAASF (p=0.01). There was statistically significant proliferation of PBMCs for MI (p=0.003) and CK (p=0.005) compared to control. However, no significant difference was observed in LAASF on proliferation. Mean absorbance scores significantly decreased with an increase in the concentration of the extracts. The extracts have potential cytotoxicity on the PBMCs at higher concentrations. The extract of MI and CK exhibited higher cytotoxic and proliferative activity on the PBMCs than LAASF. An in-depth study to identify specific immune cells proliferated by the extracts will improve the credence of this study's findings.
Medicinal plants have been widely used for medicinal purposes in the treatment of various ailment... more Medicinal plants have been widely used for medicinal purposes in the treatment of various ailments including human immunodeficiency virus/acquired immunodeficiency syndrome (HIV/AIDS). This study evaluated the effect of aqueous extracts of polyherbal formulation (PHF) on peripheral blood mononuclear cells (PBMCs) of an HIV+ patient. In this study, 4 ml of blood was collected from HIV+ patient who has not commenced antiretroviral treatment. PBMCs were isolated using the Ficoll-Paque method and counted using the trypan blue assay. The isolated PBMCs were treated with different concentrations of the PHF (25 50, 100, and 200 μg/ml). Cytotoxicity and proliferation were determined using the WST-8 assay. The percentage viability in this study was 96.74%, whereas the non-viable cell percentage was 3.26%. The lowest percentage of cytotoxicity for the PHF was 0.05% at 25 µg/ml of the extract, while the highest was 5.35% at 200 µg/ml. The mean absorbance scores of the formulation's various...
Potential mechanisms of some selected heavy metals in the induction of inflammation and autoimmunity
European Journal of Inflammation
Inflammation is a physiological event that protects tissues from infection and injury. Chronic in... more Inflammation is a physiological event that protects tissues from infection and injury. Chronic inflammation causes immune cell over activation and sustained release of inflammatory cytokines and chemokines cause pathologic conditions including autoimmune diseases. Heavy metals exposure affects innate and adaptive immune systems through triggering inflammatory responses. It seems that extended inflammatory responses could accelerate heavy metal-induced autoimmunity. In the present review we discuss the exposure route and toxicity of Cadmium (Cd), Lead (Pb), Mercury (Hg), Vanadium (V) and Platinum (Pt) and their effects on inflammatory responses by innate and adaptive immune system and autoimmunity.
Protective Activity of a Polyherbal Formulation in Cyclophosphamide-Induced Immunosuppression
Ethiopian Pharmaceutical Journal
A previous study reported on the immunostimulatory activity of a polyherbal formulation (PHF). Ho... more A previous study reported on the immunostimulatory activity of a polyherbal formulation (PHF). However, its potential in immunosuppression is unknown. This murine study evaluates the protective activity of aqueous extract of the PHF in cyclophosphamide (CP)-induced immunosuppression. The PHF consists of Acacia polyacantha, Acacia senegal, Adansonia digitata, Allium sativum and Bauhinia rufescens. The study was conducted on 20 rats in four groups: normal saline group, negative control, treatment control group, and experiment group. Delayed type hypersensitivity (DTH) response, keyhole limpet haemocyanin (KLH)-IgG, serum IFN-γ, and IL-4 were measured using digital Vernier calliper and sandwich ELISA, respectively. Histology of liver, thymus, and bone marrow was examined. No significant difference was observed between treatment control and experimental groups in DTH response (p > 0.05). No significant difference prevailed in the mean concentration of KLH-IgG from PHF treatment group...
The relationship between the level of blood pressure and BMI has been perceived to be linear and ... more The relationship between the level of blood pressure and BMI has been perceived to be linear and strong. To lay a ground we need to have an insight idea or knowledge on the issues of Hypertension and high body mass index including age as well as its going to be of high importance for us in tackling and handling the blood pressure level. This research carried out in Sharda University, examined the relationship of blood pressure, body mass index (BMI) and age among 100 patient by using a statistical tools regression and correlation, we also test the significance of the coefficient of regression. It found that age, BMI, systolic and diastolic are positively correlated and the regression model and the parameters are statistically significant. Which proved to us that there is a relationship between body mass index with blood pressure, so also age has a relationship with blood pressure but not as strong as that of body mass index.
Various species of freshwater snails act as intermediate hosts for Schistosoma species. The Bulin... more Various species of freshwater snails act as intermediate hosts for Schistosoma species. The Bulinus and Biomphalaria snails serve as intermediate hosts for Schistosoma haematobium and Schistosoma mansoni, which are the causative agents of urinary and intestinal schistosomiasis in humans, respectively. This study aims to determine the distribution and abundance of these snail intermediate hosts along Kwanar Areh Dam, Rimi Local Government Area of Katsina State, Nigeria. Three different sites were selected at Kwanar Areh dam for snail sampling. Snails were collected on monthly basis, for nine consecutive months, using scoop net technique and modified manual hand picking, and were identified using their morphological characteristics. 808 snails were collected from the three sites; 259 (32.1%), 360 (44.6%) and 189 (23.4%) snails were collected from sites A, B and C, respectively. The snails were more abundant during early rainy season and site B significantly produced the overall highes...
Fountain Journal of National ad Applied Sciences, Dec 28, 2022
Plant products provide a vast source of therapeutics, but not without toxicity. This study evalua... more Plant products provide a vast source of therapeutics, but not without toxicity. This study evaluated the in vitro cytotoxic and proliferative activity of selected plant extracts on human peripheral blood mononuclear cells (PBMCs). The PBMCs from healthy donors were exposed to varying concentrations (25 µg/ml, 50 µg/ml, 100 µg/ml and 200 µg/ml) of aqueous extracts of young leaves of Mangifera indica (MI) and stem barks of Commiphora kerstingii (CK), and Lannea acida and Acacia sieberiana formulation (LAASF). Trypan blue assay was used to determine the viability of human PBMCs after isolation. Cytotoxicity and proliferation of PBMCs were determined using WST-8 assay. The total viable cell count of the isolated PBMCs in this study was 8000 x 10 4 cells/ml while viability was 96.15%. The extract of LAASF showed the lowest percentage cytotoxicity (2.63%) at 25 µg/ml concentration, followed by CK (2.70%), then MI (7.71%). There was significant decrease in PBMCs mean absorbance scores across the different concentrations of MI (p=0.008), CK (p<0.0001) and LAASF (p=0.01). There was statistically significant proliferation of PBMCs for MI (p=0.003) and CK (p=0.005) compared to control. However, no significant difference was observed in LAASF on proliferation. Mean absorbance scores significantly decreased with an increase in the concentration of the extracts. The extracts have potential cytotoxicity on the PBMCs at higher concentrations. The extract of MI and CK exhibited higher cytotoxic and proliferative activity on the PBMCs than LAASF. An in-depth study to identify specific immune cells proliferated by the extracts will improve the credence of this study's findings.
Medicinal plants have been widely used for medicinal purposes in the treatment of various ailment... more Medicinal plants have been widely used for medicinal purposes in the treatment of various ailments including human immunodeficiency virus/acquired immunodeficiency syndrome (HIV/AIDS). This study evaluated the effect of aqueous extracts of polyherbal formulation (PHF) on peripheral blood mononuclear cells (PBMCs) of an HIV+ patient. In this study, 4 ml of blood was collected from HIV+ patient who has not commenced antiretroviral treatment. PBMCs were isolated using the Ficoll-Paque method and counted using the trypan blue assay. The isolated PBMCs were treated with different concentrations of the PHF (25 50, 100, and 200 μg/ml). Cytotoxicity and proliferation were determined using the WST-8 assay. The percentage viability in this study was 96.74%, whereas the non-viable cell percentage was 3.26%. The lowest percentage of cytotoxicity for the PHF was 0.05% at 25 µg/ml of the extract, while the highest was 5.35% at 200 µg/ml. The mean absorbance scores of the formulation's various...
Potential mechanisms of some selected heavy metals in the induction of inflammation and autoimmunity
European Journal of Inflammation
Inflammation is a physiological event that protects tissues from infection and injury. Chronic in... more Inflammation is a physiological event that protects tissues from infection and injury. Chronic inflammation causes immune cell over activation and sustained release of inflammatory cytokines and chemokines cause pathologic conditions including autoimmune diseases. Heavy metals exposure affects innate and adaptive immune systems through triggering inflammatory responses. It seems that extended inflammatory responses could accelerate heavy metal-induced autoimmunity. In the present review we discuss the exposure route and toxicity of Cadmium (Cd), Lead (Pb), Mercury (Hg), Vanadium (V) and Platinum (Pt) and their effects on inflammatory responses by innate and adaptive immune system and autoimmunity.
Protective Activity of a Polyherbal Formulation in Cyclophosphamide-Induced Immunosuppression
Ethiopian Pharmaceutical Journal
A previous study reported on the immunostimulatory activity of a polyherbal formulation (PHF). Ho... more A previous study reported on the immunostimulatory activity of a polyherbal formulation (PHF). However, its potential in immunosuppression is unknown. This murine study evaluates the protective activity of aqueous extract of the PHF in cyclophosphamide (CP)-induced immunosuppression. The PHF consists of Acacia polyacantha, Acacia senegal, Adansonia digitata, Allium sativum and Bauhinia rufescens. The study was conducted on 20 rats in four groups: normal saline group, negative control, treatment control group, and experiment group. Delayed type hypersensitivity (DTH) response, keyhole limpet haemocyanin (KLH)-IgG, serum IFN-γ, and IL-4 were measured using digital Vernier calliper and sandwich ELISA, respectively. Histology of liver, thymus, and bone marrow was examined. No significant difference was observed between treatment control and experimental groups in DTH response (p > 0.05). No significant difference prevailed in the mean concentration of KLH-IgG from PHF treatment group...
The relationship between the level of blood pressure and BMI has been perceived to be linear and ... more The relationship between the level of blood pressure and BMI has been perceived to be linear and strong. To lay a ground we need to have an insight idea or knowledge on the issues of Hypertension and high body mass index including age as well as its going to be of high importance for us in tackling and handling the blood pressure level. This research carried out in Sharda University, examined the relationship of blood pressure, body mass index (BMI) and age among 100 patient by using a statistical tools regression and correlation, we also test the significance of the coefficient of regression. It found that age, BMI, systolic and diastolic are positively correlated and the regression model and the parameters are statistically significant. Which proved to us that there is a relationship between body mass index with blood pressure, so also age has a relationship with blood pressure but not as strong as that of body mass index.
Various species of freshwater snails act as intermediate hosts for Schistosoma species. The Bulin... more Various species of freshwater snails act as intermediate hosts for Schistosoma species. The Bulinus and Biomphalaria snails serve as intermediate hosts for Schistosoma haematobium and Schistosoma mansoni, which are the causative agents of urinary and intestinal schistosomiasis in humans, respectively. This study aims to determine the distribution and abundance of these snail intermediate hosts along Kwanar Areh Dam, Rimi Local Government Area of Katsina State, Nigeria. Three different sites were selected at Kwanar Areh dam for snail sampling. Snails were collected on monthly basis, for nine consecutive months, using scoop net technique and modified manual hand picking, and were identified using their morphological characteristics. 808 snails were collected from the three sites; 259 (32.1%), 360 (44.6%) and 189 (23.4%) snails were collected from sites A, B and C, respectively. The snails were more abundant during early rainy season and site B significantly produced the overall highes...
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