Papers by Abdelrahman Elbagory
Tropical Animal Health and Production
The current study was conducted to evaluate the immunoenhancement effect of Moringa oleifera leav... more The current study was conducted to evaluate the immunoenhancement effect of Moringa oleifera leaves alcoholic extract (MOLE) versus Oregano essential oil (OEO) against cyclophosphamide induced immunosuppression in broilers chicks. A total of a three hundred one-day-old chicks were assigned randomly into three main dietary groups, control, MOLE, and OEO for 14 days. After 14 days the three main experimental groups were subdivided into six groups, control, cyclophosphamide, MOLE, MOLE and Cyclophosphamide, OEO, and OEO and cyclophosphamide. Each group of these six groups was subdivided into three subgroups. Supplementation of broiler chicks with MOLE and OEO for 14 days significantly increased body weight compared to the control group. However, injection of broiler chicks with cyclophosphamide significantly induced body weight loss, impaired immunological response represented by decreasing total leukocytic count, differential leukocytic count, phagocytic activity, phagocytic index, an...
Veterinary Immunology and Immunopathology, 2007
Flow cytometry was used to screen a panel of 320 mAbs, submitted to the Animal Homologues Section... more Flow cytometry was used to screen a panel of 320 mAbs, submitted to the Animal Homologues Section of the HLDA8, for mAbs that recognize epitopes conserved on orthologous leukocyte differentiation antigens (LDA) in goats, lamas, and rabbits. Nineteen mAbs specific for CD11a (1), CD14 (3), CD18 (1), CD21 (1), CD29 (2), CD44 (2), CD47 (3), CD49d (1), CD172a (1), CD45RB (1), CD61 (1), RACT48A, and GBSP71A reacted with goat LDA. Twenty three mAbs specific for CD7 (1), CD9 (2), CD11a (1), CD14 (3), CD18 (4), CD29 (1), CD32 (1), CD44 (1), CD47 (4), CD49d (2), CD50 (1), CD80 (1), CD172a (1), and GBSP71A reacted with llama LDA. Eighteen mAbs specific for CD9 (2), CD11a (1), CD14 (2), CD18 (4), CD21 (1), CD44 (2), CD45RB (1), CD49d (1), CD209 (1), RACT48A, and GBSP71A reacted with rabbit LDA. The specificities of two cross reactive mAbs that recognize different conserved epitopes on all leukocytes in two species (RACT48A) and all three species (GBSP71A) have not been determined. The patterns of reactivity of most of the mAbs were consistent with patterns of reactivity noted on human leukocytes. The specificity of some cross reactive mAbs generated in non-human species were validated on human leukocytes. Further studies are needed to verify that CD7, CD32, CD45RB, CD50, and CD209 recognize orthologous molecules in the indicated species.
Livestock Science, 2015
ABSTRACT The presented study investigated changes of fat cell and muscle fibre size, and muscle f... more ABSTRACT The presented study investigated changes of fat cell and muscle fibre size, and muscle fibre type composition around first parturition in cows with a combined beef and dairy genetic background. In contrast to high-merit dairy cows, these cows can serve as a model for beef cows because of the very low milk production level. Fifty heifers used in this experiment were F2 offspring originating from mating Charolais bulls to German Holstein cows and a following intercross of F1 individuals. They were assigned to 3 groups according to their lactation performance, namely high lactating (HL), low lactating long (LLL; duration of first lactation at least 100 days), and low lactation short (LLS; duration of first lactation shorter than 100 days). Biopsy samples were taken from semitendinosus muscle (MST) and subcutaneous fat (SCF) at three time points, 10 days before first parturition, 30 and 100 days after calving. These samples were investigated by histology and computer image analysis for muscle fibre profile, as well as intramuscular and subcutaneous fat cell size to estimate the impact of lactation on body reserves. Most cows continued growth and gained weight during lactation. Continuous tissue accretion was indicated on the cellular level by an increase in intramuscular fat cell size. Subcutaneous fat cells were larger in low lactating cows with a short lactation period. There were no morphological signs of fat mobilization from fat cells. There was also no protein mobilization indicated by changes in muscle fibre size. However, the increase in percentage of fast, glycolytic muscle fibres indicated alterations in muscle metabolism during transition from late pregnancy to early lactation. Cows remained obviously in a positive energy balance in this study and continued tissue accretion during lactation. This was reflected on the cellular level in skeletal muscle and subcutaneous fat.
Two hundred samples, 40 each, of raw milk , small scale yoghurt, large scale yoghurt, small scale... more Two hundred samples, 40 each, of raw milk , small scale yoghurt, large scale yoghurt, small scale ice cream, and large scale ice cream were collected from supermarkets, dairy shops and street vendors in El-Minoufiya governorate for microbiological analysis . Verotoxigenic E.coli O157:H7 could be isolated from one raw milk sample (2.5%). Seriological typing proved that, the isolated strain has the antigenic structure of O157:H7 . Other verotoxigenic E.coli could be isolated from examined raw milk (O128,O119,O26,O25) and yoghurt (O26) samples .Isolated verotoxigenic E.coli O157:H7 and other verotoxigenic E.coli proved to be carrier for verotoxin II encoding gene using polymerase chain reaction . Public health significance as well as suggestive control measures of these organisms were discussed.
Abstract This study aimed to investigate samples of Kariesh and Ras cheese collected from Menofia... more Abstract This study aimed to investigate samples of Kariesh and Ras cheese collected from Menofia Governorate for their contamination with enterobacteriaceae and document the presence or absence of its potential pathogens specifically Salmonella and E. coli. Examination of 120 samples of kariesh and ras cheese revealed that the enterobacteriaceae were present in 100% and 51.67% of examined karish and ras cheese samples respectively. The min, max and mean values of enterobacteriaceace of karish cheese were 9.00 X 103, 1.23 X107 &16x105 ± 3x105 /g while that of ras cheese were 0,00, 50 x104 & 21x103 ± 10x103 /g respectively. E. coli was present in 31.67% &16.67% of examined karish and ras cheese samples respectively. Isolated serovars of E. coli were belonging to EHEC, ETEC and EPEC groups. Different members of family enterobacteriaceae of public health importance were isolated from examined samples. Salmonella spp couldn’t be detected in all examined cheese samples
Consumption of camel’s milk in Egypt is increasing and mainly consumed in its raw state. So this ... more Consumption of camel’s milk in Egypt is increasing and mainly consumed in its raw state. So this study planned to investigate the bacteriological quality of camel milk and the possible presence of some milk borne pathogens as E. coli S. aureus, Salmonella spp. and Listeria monocytogenes.Thirty five composite raw camel milk samples were collected from a camel farm in Matrouh Governorate, Egypt. The mean value of aerobic plate count (APC) was 1.19× 10^7±2.54×10^6 cfu/ml, while the mean count value of Enterobacteriaceae was 2.72×10^6±3.91×10^5 cfu/ml. Coliforms was detected in 88.57% of the examined samples, with mean count value of 8.49×10^5±7.84×10^4 cfu/ml. E. coli was isolated from 25.71% of examined samples. The isolated E. coli belonged to three serotypes: O119, O124 and untypable. S. aureus was detected in 42.86% of the examined samples with mean count value of 2.4×10^5±1×10^5 cfu/ml. Salmonella and Listeria monocytogenes were not detected in the examined samples.
A total of 250 random meat product samples (50 each of minced meat, beef burger, beef sausage, be... more A total of 250 random meat product samples (50 each of minced meat, beef burger, beef sausage, beef kofta and beef luncheon) were collected from different super markets at Menofia, Quliobia and Cairo governorates for detection of Escherichia coli using conventional method and Polymerase chain reaction. Conventional method indicated that E. coli could be isolated from of minced meat, beef burger, beef sausage, beef kofta and beef luncheon in percentage of 46%, 28%, 32% , 36% and 16% respectively. While by using Polymerase chain reaction(PCR) detected that E. coli in minced meat, beef burger, beef sausage, beef kofta and beef luncheon in a percentage of 38%, 22%, 30%, 32% and 12% respectively. In conclusion meat products constitute an important reservoir of Escherichia coli infection to man and PCR reaction is the most rapid, sensitive and efficient approach for detection of E. coli.
Archives of Virology, 2009
During 2004–2006 swine influenza virus surveillance, two strains of H3N8 influenza viruses were i... more During 2004–2006 swine influenza virus surveillance, two strains of H3N8 influenza viruses were isolated from pigs in central China. Sequence and phylogenetic analyses of eight gene segments revealed that the two swine isolates were of equine origin and most closely related to European equine H3N8 influenza viruses from the early 1990s. Comparison of hemagglutinin (HA) amino acid sequences showed several
A total of 2177 food samples collected from nine cities in northern China during 2005 to 2007 wer... more A total of 2177 food samples collected from nine cities in northern China during 2005 to 2007 were screened for the presence of Listeria monocytogenes. All L. monocytogenes isolates were subjected to serotyping, antimicrobial susceptibility, pulsed-field gel electrophoresis (PFGE), as well as PCR screening to identify genes responsible for tetracycline resistance [tet(L), tet(M), tet(K), tet(S) and tet(B)], transposon Tn916, and class 1 integron. Contamination with L. monocytogenes was detected in 4.13% (90/2177) of the total samples representing various food products. The pathogen was mainly isolated from frozen food made of wheat flour or rice products (26/252, 10.32%) and raw meat products (46/733, 6.28%). Besides, 3.31% (10/302) of cooked meat, 1.17% (4/343) of seafood, 0.98% (2/204) of non-fermented bean products and 0.62% (2/323) of vegetables samples were contaminated by this bacterium. The L. monocytogenes isolates belonged to five serotypes (1/2a, 1/2b, 1/2c, 4b, and 3a), with serotype 1/2a being dominant (48.88%). Antimicrobial resistance was most frequently observed for ciprofloxacin (17.8%), tetracycline (15.6%) and streptomycin (12.2%). Overall, resistance was observed against 14 out of 18 antimicrobials tested while multiple resistances occurred among 18.9% (17/90) isolates. Interestingly, two isolates were resistant to more than five antimicrobials. Among 14 tetracycline-resistant isolates, 13 carried tet(M) gene including nine possessing Tn916, and one harbored tet(S) gene. PFGE analysis revealed genetic heterogeneity among individual serotypes as well as scattered occurrence of some genotypes without any clear-cut correlation to source or food type. The widespread distribution of epidemiologically important serotypes (1/2a, 1/2b and 4b) of L. monocytogenes, and their resistance to commonly used antibiotics indicate a potential public health risk. Our data also indicate that L. monocytogenes could act as a reservoir of mobile tet genes along the food chain.
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Papers by Abdelrahman Elbagory