101-106<span style="font-size:11.0pt;font-family: " times="" new="&qu... more 101-106<span style="font-size:11.0pt;font-family: " times="" new="" roman";mso-fareast-font-family:"times="" roman";mso-bidi-font-family:="" mangal;mso-ansi-language:en-gb;mso-fareast-language:en-us;mso-bidi-language:="" hi"="" lang="EN-GB">Salinity stress affects adversely to the plants, resulting in their growth inhibition. In this article, we describe changes in dry wt, root length, ion content and proteome in the roots of Azolla microphylla exposed to salinity (0.5%). The exposed plants exhibited shorter roots as compared to their control. The sodium content of exposed roots was increased, whereas the potassium content did not show any significant and appreciable change. Of the approx 350 protein spots detected using 2-dimensional electrophoresis, 32 spots indicated significant changes. Seven of these 32 proteins were identified by MALDI-TOF-MS/MS analysis and were found to ...
International Journal of Engineering Applied Sciences and Technology, 2022
Dietary fiber is that part of plant material in the diet which is resistant to enzymatic digestio... more Dietary fiber is that part of plant material in the diet which is resistant to enzymatic digestion which includes cellulose, non-cellulosic polysaccharides such as hemicellulose, pectic substances, gums, mucilages and a non-carbohydrate component lignin. The diets rich in fiber such as cereals, nuts, fruits and vegetables have a positive effect on health since their consumption has been related to decreased incidence of several diseases. Dietary fiber can be used in various functional foods like bakery, drinks, beverages and meat products. Influence of different processing treatments (like extrusioncooking, canning, grinding, boiling, frying) alters the physico-chemical properties of dietary fiber and improves their functionality. An attempt has been made to compile the recent developments in the extraction, applications and functions of dietary fiber in different food products.
Indian Journal of Agricultural Sciences, Jul 26, 2021
Delhi (2017-18) to study the possible reason for reduced seed vigour in Indian quality mustard ge... more Delhi (2017-18) to study the possible reason for reduced seed vigour in Indian quality mustard genotypes during storage for the first time. Freshly harvested seeds, one year, two year aged seeds and controlled deteriorated seeds of freshly harvested seeds of conventional and quality Indian mustard were used for the study. Reactive oxygen species (ROS), i.e. hydrogen peroxide (H 2 O 2) and superoxide radical (O 2 .-) were estimated along with enzymatic antioxidants such as catalase and peroxidase and redox couples ratio (ASC/DHA and GSH/GSSG) in all the nine genotypes of different age group and correlated with germination percentage, mean germination time and seed vigour indices. The experiment revealed that during storage, the amount of ROS accumulation goes on increasing and both enzymatic and non-enzymatic antioxidants goes on decreasing. In comparison to conventional genotypes, quality genotypes were found to contain significantly lower level of antioxidants and significantly higher level of ROS content during the storage period. Germination percentage as well as seed vigour indices were negatively correlated with ROS and positively correlated with enzymatic and non-enzymatic antioxidants. This experiment revealed that reduced vigour in quality genotypes could be due to faster deterioration during the storage due to increased production of ROS.
Background: In recent years, there has been an increase in consumer demand for beverages made fro... more Background: In recent years, there has been an increase in consumer demand for beverages made from fermented soybeans. Unfortunately, the nutritional imbalance and flavour profiles of plant-based milk substitutes frequently prevent people from consuming them. Fermentation can aid plant-based milk substitutes' taste profiles, nutritional characteristics, texture and microbiological safety in order to produce more valued and delicious products without adding additional, frequently viewed as fake, ingredients. Methods: The implications of probiotic fermentation to improve the nutritive and sensory quality of fermented soymilk are assessed. Three soybean varieties as food grade soybean (Krune), low KTI soybean (V-23) and black soybean (VL-Bhatt 65) are used for the formation of soymilk which further fermented by three probiotic strains as Lactobacillus rhamnosus, Lactobacillus acidophilus and Weissella confusa. The fermented soymilk samples were stored for 28 th days at 4C to analyse the changes in chemical composition and sensory quality. Result: High protein content was found in BA (6.85±0.13 g/100g), while the consumer preferred the LR with best overall ranking (6.25±0.05) in hedonic scale. The viability count of probiotic was highest (9.44±0.17 log cfu/ml) in BA after storage. The study suggested that soymilk with enormous number of biomolecules or pigments showed better viability of probiotic after storage and L. rhamnosus fermented soymilk preferred by the consumer over others probiotic fermented soymilk.
The response given by the photosynthetic pigments, antioxidant enzyme activity and nutrient accum... more The response given by the photosynthetic pigments, antioxidant enzyme activity and nutrient accumulation of kinnow (Citrus nobilis × C. deliciosa) budded on Citrus jambhiri Lush rootstocks (var. Jatti Khatti) to triazole compounds was studied under NaCl stress. Eighteen-month-old budded kinnow plants were irrigated with 0 or 50 mM NaCl with or without triazole compounds. Paclobutrazol (PBZ) at 100 mg 1-1 and triazole (TRZ), triadimefon (TDM) and hexaconazole (HEX) at 20 mg l-1 were applied by drenching the soil one week prior to NaCl application. PBZ, TRZ and TDM were equally effective in preventing chlorophyll degradation under NaCl stress. The improvement in catalase (CAT) activity was greater in TRZ-and TDM-treated plants under stress, while for superoxide dismutase (SOD) activity, TRZ, PBZ and TDM were found to be equally effective. The peroxidase (POX) activity increased more in TDM-treated plants. The application of TDM and HEX had a more pronounced effect on the upregulation of proline content under stress. Furthermore, triazole compounds increased the accumulation of K + and Ca 2+ ions and inhibited that of Na + and Cl-, resulting in an improvement in the K + /Na + and K + /Clratios. For PBZ-treated plants, the K + /Na + and K + /Clratios in leaf tissues increased 2.4and 2.1-fold compared to plants treated with NaCl alone, while HEX had a more striking effect on Ca 2+ upregulation and significantly (1.5-fold) increased leaf Ca 2+ under stress.
High potential is attributed to the concomitant use of probiotics and prebiotics in a single food... more High potential is attributed to the concomitant use of probiotics and prebiotics in a single food product, called "synbiotics," where the prebiotic component distinctly favours the growth and activity of probiotic microbes. This study implemented a detailed comparison between the prebiotic effect of Fructooligosaccharides (FOS) and Raffinose Oligosaccharides (RFOs) on the viable count of bacteria, hydrolysis into monosaccharides for the biosynthesis of postbiotic Short Chain Fatty Acids and sensory attributes of soymilk fermented with 1% (v/v) mixed cultures of Lacticaseibacillus rhamnosus JCM 1136 and Weissella confusa 30082b. The highest viable count of 1.21×109 CFU/ml was observed in soymilk with 3%RFOs added as a prebiotic source compared with MRS broth with 3% RFOs (3.21×108) and 3%FOS (6.2 ×107 CFU/ml) when replaced against glucose in MRS broth. Raffinose and stachyose were extensively metabolised (4.75 and 1.28-fold decrease, respectively) in 3%RFOs supplemented wit...
India is one of the leading citrus−producing countries, next to China and Brazil. Despite the siz... more India is one of the leading citrus−producing countries, next to China and Brazil. Despite the sizeable production, especially of lemons and limes, India contributes meagerly to the world’s citrus market. Of the various factors responsible for the restricted quantum of citrus export, citrus canker (Xanthomonas citri pv. citri) is one of the leading serious causes and directly impacts the appearance of the fruits. Considering the extent of damage, the present study aimed to understand the impact of Xanthomonas citri pv. citri (Xcc) on the physio−biochemical responses in contrasting Citrus spp. Two genotypes, each of three citrus species, namely Citrus aurantifolia (Pusa Udit and ALC−35), C. limon (Kagzi Kalan and Konkan Seedless), and C. paradisi (Redblush and Marsh Seedless), were artificially inoculated with Xcc (108 to 109 cfu/ mL) by the pinprick method. The physio−biochemical changes in the host were evaluated after 48 h post inoculation (hpi). The chlorophyll content (total, a, ...
Apple Cultivation - Recent Advances [Working Title]
The naturally occurring phenolic compounds have received major attention in recent years as huge ... more The naturally occurring phenolic compounds have received major attention in recent years as huge amounts of phenolic compounds can be extracted from fruits, vegetables and beverages that have substantial health benefits. From a physiological and metabolic aspect, phenolic compounds are vital in defence responses, such as anti-ageing, anti-inflammatory, anti-oxidant and anti-proliferative, anti-bacterial, anti-hyperlipidemic, anti-cancer, anti-diabetic, neuroprotective, cardioprotective activities. Among the fruits having a higher content of phenolic compounds, the apple (Malus Domestica) is the most widely consumed fruit in the world. Apples have a high nutritional value as it is a rich source of ascorbic acid, polyphenols and pectin. Apple peel forms a small percentage (6–8%) of the total fruit weight and contains the highest content of phenolic compounds, particularly chlorogenic acid. There are five major groups of polyphenolic compounds found in apples namely flavanols (Catechin...
The entomopathogenic nematode, Heterorhabditis indica, is a popular biocontrol agent of high comm... more The entomopathogenic nematode, Heterorhabditis indica, is a popular biocontrol agent of high commercial significance. It possesses tremendous genetic architecture to survive desiccation stress by undergoing anhydrobiosis to increase its lifespan—an attribute exploited in the formulation technology. The comparative transcriptome of unstressed and anhydrobiotic H. indica revealed several previously concealed metabolic events crucial for adapting towards the moisture stress. During the induction of anhydrobiosis in the infective juveniles (IJ), 1584 transcripts were upregulated and 340 downregulated. As a strategy towards anhydrobiotic survival, the IJ showed activation of several genes critical to antioxidant defense, detoxification pathways, signal transduction, unfolded protein response and molecular chaperones and ubiquitin-proteasome system. Differential expression of several genes involved in gluconeogenesis - β-oxidation of fatty acids, glyoxylate pathway; glyceroneogenesis; fat...
Soybean (Glycine max (L.) Merr) is a nutrient-rich crop with limited consumer acceptability becau... more Soybean (Glycine max (L.) Merr) is a nutrient-rich crop with limited consumer acceptability because of the presence of off-flavour. Owing to the ubiquity of isoflavones in soybean, their inherent antioxidant potential, and their inhibitory effect on lipoxygenase activity, their sensorial attribute in mitigating off-flavour is currently being envisaged. We estimated the content and composition of isoflavones in 17 soybean cultivars and established the correlation between isoflavone profile and lipid peroxidation, which was measured in terms of thiobarbituric acid (TBA) number and carbonyl value. The results showed a significant negative correlation of these parameters with the total isoflavone content and genistein: daidzein ratio. In addition, expression analysis of a key gene involved in isoflavone biosynthesis, i.e., isoflavone synthase (IFS1 and IFS2), in contrasting soybean genotypes during seed development revealed a higher expression of both isoforms in Bragg (high isoflavone)...
An Agrobacterium-mediated transformation method optimized for two high yielding/popular Indian so... more An Agrobacterium-mediated transformation method optimized for two high yielding/popular Indian soybean genotypes JS335 and DS 9712, using the cotyledonary node (CN) and hypocotyl segment systems. The explants were co-cultivated with Agrobacterium tumefaciens harboring the binary vector with bar (phosphinothricin acetyltransferase gene, a selectable marker gene) and the gus reporter gene. The results revealed a higher regeneration frequency using CN as explants in the both cultivars JS335 (75%) and DS 9712 (50%), as compared to regeneration frequency of 44% (JS335) and 27% (DS 9712) using hypocotyl segments as explants. An addition of dithiothreitol (DTT) and sodium thiosulfate along with Lcysteine during co-cultivation of JS-335 showed an increase (33%) in the transformation efficiency. Shoot induction on the nonselective shoot induction media (SIM) for 10 days before transferring the explants to SIM containing 5 mg/L of glufosinate greatly stimulated the regeneration of the transfo...
Phenolic compounds from plant sources have significant health-promoting properties and are known ... more Phenolic compounds from plant sources have significant health-promoting properties and are known to be an integral part of folk and herbal medicines. Consumption of phenolics is known to alleviate the risk of various lifestyle diseases including cancer, cardiovascular, diabetes, and Alzheimer's. In this context, numerous plant crops have been explored and characterized based on phenolic compounds for their use as supplements, nutraceutical, and pharmaceuticals. The present review highlights some important source of bioactive phenolic compounds and novel technologies for their efficient extraction. These techniques include the use of microwave, ultrasound, and supercritical methods. Besides, the review will also highlight the use of response surface methodology (RSM) as a statistical tool for optimizing the recoveries of the phenolic bioactives from plant-based matrices.
101-106<span style="font-size:11.0pt;font-family: " times="" new="&qu... more 101-106<span style="font-size:11.0pt;font-family: " times="" new="" roman";mso-fareast-font-family:"times="" roman";mso-bidi-font-family:="" mangal;mso-ansi-language:en-gb;mso-fareast-language:en-us;mso-bidi-language:="" hi"="" lang="EN-GB">Salinity stress affects adversely to the plants, resulting in their growth inhibition. In this article, we describe changes in dry wt, root length, ion content and proteome in the roots of Azolla microphylla exposed to salinity (0.5%). The exposed plants exhibited shorter roots as compared to their control. The sodium content of exposed roots was increased, whereas the potassium content did not show any significant and appreciable change. Of the approx 350 protein spots detected using 2-dimensional electrophoresis, 32 spots indicated significant changes. Seven of these 32 proteins were identified by MALDI-TOF-MS/MS analysis and were found to ...
International Journal of Engineering Applied Sciences and Technology, 2022
Dietary fiber is that part of plant material in the diet which is resistant to enzymatic digestio... more Dietary fiber is that part of plant material in the diet which is resistant to enzymatic digestion which includes cellulose, non-cellulosic polysaccharides such as hemicellulose, pectic substances, gums, mucilages and a non-carbohydrate component lignin. The diets rich in fiber such as cereals, nuts, fruits and vegetables have a positive effect on health since their consumption has been related to decreased incidence of several diseases. Dietary fiber can be used in various functional foods like bakery, drinks, beverages and meat products. Influence of different processing treatments (like extrusioncooking, canning, grinding, boiling, frying) alters the physico-chemical properties of dietary fiber and improves their functionality. An attempt has been made to compile the recent developments in the extraction, applications and functions of dietary fiber in different food products.
Indian Journal of Agricultural Sciences, Jul 26, 2021
Delhi (2017-18) to study the possible reason for reduced seed vigour in Indian quality mustard ge... more Delhi (2017-18) to study the possible reason for reduced seed vigour in Indian quality mustard genotypes during storage for the first time. Freshly harvested seeds, one year, two year aged seeds and controlled deteriorated seeds of freshly harvested seeds of conventional and quality Indian mustard were used for the study. Reactive oxygen species (ROS), i.e. hydrogen peroxide (H 2 O 2) and superoxide radical (O 2 .-) were estimated along with enzymatic antioxidants such as catalase and peroxidase and redox couples ratio (ASC/DHA and GSH/GSSG) in all the nine genotypes of different age group and correlated with germination percentage, mean germination time and seed vigour indices. The experiment revealed that during storage, the amount of ROS accumulation goes on increasing and both enzymatic and non-enzymatic antioxidants goes on decreasing. In comparison to conventional genotypes, quality genotypes were found to contain significantly lower level of antioxidants and significantly higher level of ROS content during the storage period. Germination percentage as well as seed vigour indices were negatively correlated with ROS and positively correlated with enzymatic and non-enzymatic antioxidants. This experiment revealed that reduced vigour in quality genotypes could be due to faster deterioration during the storage due to increased production of ROS.
Background: In recent years, there has been an increase in consumer demand for beverages made fro... more Background: In recent years, there has been an increase in consumer demand for beverages made from fermented soybeans. Unfortunately, the nutritional imbalance and flavour profiles of plant-based milk substitutes frequently prevent people from consuming them. Fermentation can aid plant-based milk substitutes' taste profiles, nutritional characteristics, texture and microbiological safety in order to produce more valued and delicious products without adding additional, frequently viewed as fake, ingredients. Methods: The implications of probiotic fermentation to improve the nutritive and sensory quality of fermented soymilk are assessed. Three soybean varieties as food grade soybean (Krune), low KTI soybean (V-23) and black soybean (VL-Bhatt 65) are used for the formation of soymilk which further fermented by three probiotic strains as Lactobacillus rhamnosus, Lactobacillus acidophilus and Weissella confusa. The fermented soymilk samples were stored for 28 th days at 4C to analyse the changes in chemical composition and sensory quality. Result: High protein content was found in BA (6.85±0.13 g/100g), while the consumer preferred the LR with best overall ranking (6.25±0.05) in hedonic scale. The viability count of probiotic was highest (9.44±0.17 log cfu/ml) in BA after storage. The study suggested that soymilk with enormous number of biomolecules or pigments showed better viability of probiotic after storage and L. rhamnosus fermented soymilk preferred by the consumer over others probiotic fermented soymilk.
The response given by the photosynthetic pigments, antioxidant enzyme activity and nutrient accum... more The response given by the photosynthetic pigments, antioxidant enzyme activity and nutrient accumulation of kinnow (Citrus nobilis × C. deliciosa) budded on Citrus jambhiri Lush rootstocks (var. Jatti Khatti) to triazole compounds was studied under NaCl stress. Eighteen-month-old budded kinnow plants were irrigated with 0 or 50 mM NaCl with or without triazole compounds. Paclobutrazol (PBZ) at 100 mg 1-1 and triazole (TRZ), triadimefon (TDM) and hexaconazole (HEX) at 20 mg l-1 were applied by drenching the soil one week prior to NaCl application. PBZ, TRZ and TDM were equally effective in preventing chlorophyll degradation under NaCl stress. The improvement in catalase (CAT) activity was greater in TRZ-and TDM-treated plants under stress, while for superoxide dismutase (SOD) activity, TRZ, PBZ and TDM were found to be equally effective. The peroxidase (POX) activity increased more in TDM-treated plants. The application of TDM and HEX had a more pronounced effect on the upregulation of proline content under stress. Furthermore, triazole compounds increased the accumulation of K + and Ca 2+ ions and inhibited that of Na + and Cl-, resulting in an improvement in the K + /Na + and K + /Clratios. For PBZ-treated plants, the K + /Na + and K + /Clratios in leaf tissues increased 2.4and 2.1-fold compared to plants treated with NaCl alone, while HEX had a more striking effect on Ca 2+ upregulation and significantly (1.5-fold) increased leaf Ca 2+ under stress.
High potential is attributed to the concomitant use of probiotics and prebiotics in a single food... more High potential is attributed to the concomitant use of probiotics and prebiotics in a single food product, called "synbiotics," where the prebiotic component distinctly favours the growth and activity of probiotic microbes. This study implemented a detailed comparison between the prebiotic effect of Fructooligosaccharides (FOS) and Raffinose Oligosaccharides (RFOs) on the viable count of bacteria, hydrolysis into monosaccharides for the biosynthesis of postbiotic Short Chain Fatty Acids and sensory attributes of soymilk fermented with 1% (v/v) mixed cultures of Lacticaseibacillus rhamnosus JCM 1136 and Weissella confusa 30082b. The highest viable count of 1.21×109 CFU/ml was observed in soymilk with 3%RFOs added as a prebiotic source compared with MRS broth with 3% RFOs (3.21×108) and 3%FOS (6.2 ×107 CFU/ml) when replaced against glucose in MRS broth. Raffinose and stachyose were extensively metabolised (4.75 and 1.28-fold decrease, respectively) in 3%RFOs supplemented wit...
India is one of the leading citrus−producing countries, next to China and Brazil. Despite the siz... more India is one of the leading citrus−producing countries, next to China and Brazil. Despite the sizeable production, especially of lemons and limes, India contributes meagerly to the world’s citrus market. Of the various factors responsible for the restricted quantum of citrus export, citrus canker (Xanthomonas citri pv. citri) is one of the leading serious causes and directly impacts the appearance of the fruits. Considering the extent of damage, the present study aimed to understand the impact of Xanthomonas citri pv. citri (Xcc) on the physio−biochemical responses in contrasting Citrus spp. Two genotypes, each of three citrus species, namely Citrus aurantifolia (Pusa Udit and ALC−35), C. limon (Kagzi Kalan and Konkan Seedless), and C. paradisi (Redblush and Marsh Seedless), were artificially inoculated with Xcc (108 to 109 cfu/ mL) by the pinprick method. The physio−biochemical changes in the host were evaluated after 48 h post inoculation (hpi). The chlorophyll content (total, a, ...
Apple Cultivation - Recent Advances [Working Title]
The naturally occurring phenolic compounds have received major attention in recent years as huge ... more The naturally occurring phenolic compounds have received major attention in recent years as huge amounts of phenolic compounds can be extracted from fruits, vegetables and beverages that have substantial health benefits. From a physiological and metabolic aspect, phenolic compounds are vital in defence responses, such as anti-ageing, anti-inflammatory, anti-oxidant and anti-proliferative, anti-bacterial, anti-hyperlipidemic, anti-cancer, anti-diabetic, neuroprotective, cardioprotective activities. Among the fruits having a higher content of phenolic compounds, the apple (Malus Domestica) is the most widely consumed fruit in the world. Apples have a high nutritional value as it is a rich source of ascorbic acid, polyphenols and pectin. Apple peel forms a small percentage (6–8%) of the total fruit weight and contains the highest content of phenolic compounds, particularly chlorogenic acid. There are five major groups of polyphenolic compounds found in apples namely flavanols (Catechin...
The entomopathogenic nematode, Heterorhabditis indica, is a popular biocontrol agent of high comm... more The entomopathogenic nematode, Heterorhabditis indica, is a popular biocontrol agent of high commercial significance. It possesses tremendous genetic architecture to survive desiccation stress by undergoing anhydrobiosis to increase its lifespan—an attribute exploited in the formulation technology. The comparative transcriptome of unstressed and anhydrobiotic H. indica revealed several previously concealed metabolic events crucial for adapting towards the moisture stress. During the induction of anhydrobiosis in the infective juveniles (IJ), 1584 transcripts were upregulated and 340 downregulated. As a strategy towards anhydrobiotic survival, the IJ showed activation of several genes critical to antioxidant defense, detoxification pathways, signal transduction, unfolded protein response and molecular chaperones and ubiquitin-proteasome system. Differential expression of several genes involved in gluconeogenesis - β-oxidation of fatty acids, glyoxylate pathway; glyceroneogenesis; fat...
Soybean (Glycine max (L.) Merr) is a nutrient-rich crop with limited consumer acceptability becau... more Soybean (Glycine max (L.) Merr) is a nutrient-rich crop with limited consumer acceptability because of the presence of off-flavour. Owing to the ubiquity of isoflavones in soybean, their inherent antioxidant potential, and their inhibitory effect on lipoxygenase activity, their sensorial attribute in mitigating off-flavour is currently being envisaged. We estimated the content and composition of isoflavones in 17 soybean cultivars and established the correlation between isoflavone profile and lipid peroxidation, which was measured in terms of thiobarbituric acid (TBA) number and carbonyl value. The results showed a significant negative correlation of these parameters with the total isoflavone content and genistein: daidzein ratio. In addition, expression analysis of a key gene involved in isoflavone biosynthesis, i.e., isoflavone synthase (IFS1 and IFS2), in contrasting soybean genotypes during seed development revealed a higher expression of both isoforms in Bragg (high isoflavone)...
An Agrobacterium-mediated transformation method optimized for two high yielding/popular Indian so... more An Agrobacterium-mediated transformation method optimized for two high yielding/popular Indian soybean genotypes JS335 and DS 9712, using the cotyledonary node (CN) and hypocotyl segment systems. The explants were co-cultivated with Agrobacterium tumefaciens harboring the binary vector with bar (phosphinothricin acetyltransferase gene, a selectable marker gene) and the gus reporter gene. The results revealed a higher regeneration frequency using CN as explants in the both cultivars JS335 (75%) and DS 9712 (50%), as compared to regeneration frequency of 44% (JS335) and 27% (DS 9712) using hypocotyl segments as explants. An addition of dithiothreitol (DTT) and sodium thiosulfate along with Lcysteine during co-cultivation of JS-335 showed an increase (33%) in the transformation efficiency. Shoot induction on the nonselective shoot induction media (SIM) for 10 days before transferring the explants to SIM containing 5 mg/L of glufosinate greatly stimulated the regeneration of the transfo...
Phenolic compounds from plant sources have significant health-promoting properties and are known ... more Phenolic compounds from plant sources have significant health-promoting properties and are known to be an integral part of folk and herbal medicines. Consumption of phenolics is known to alleviate the risk of various lifestyle diseases including cancer, cardiovascular, diabetes, and Alzheimer's. In this context, numerous plant crops have been explored and characterized based on phenolic compounds for their use as supplements, nutraceutical, and pharmaceuticals. The present review highlights some important source of bioactive phenolic compounds and novel technologies for their efficient extraction. These techniques include the use of microwave, ultrasound, and supercritical methods. Besides, the review will also highlight the use of response surface methodology (RSM) as a statistical tool for optimizing the recoveries of the phenolic bioactives from plant-based matrices.
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