Papers by Amado Salvador Peña
Background: Patients with inflammatory bowel disease (IBD) have an increased risk of venous throm... more Background: Patients with inflammatory bowel disease (IBD) have an increased risk of venous thromboembolisms and recurrent thrombotic disease compared with the general population. Plasma coagulation factor XIII activity has been reported to be decreased in IBD. Two common non-synonymous polymorphisms in the gene encoding coagulation factor XIII, A1 polypeptide F13A1 Val34Leu and F13A1 Pro564Leu are associated with thrombotic diseases. We investigated the role of these gene polymorphisms in the susceptibility to and severity of IBD. Methods: We genotyped 666 unrelated Dutch IBD patients (338 suffering from ulcerative colitis (UC) and 328 suffering from Crohn's disease (CD)) and 190 ethnically matched healthy subjects by a Polymerase Chain Reaction–Restriction Fragment Length Polymorphism method. UC was classified according to the colonic extent of inflammation. CD was classified according to the Vienna classification.
Revista Española de Enfermedades Digestivas, 2005
Arthralgias and spondyloarthropathies of the peripheral and axial joints are common in inflammato... more Arthralgias and spondyloarthropathies of the peripheral and axial joints are common in inflammatory bowel disease. Evidence for a strong association between these clinical manifestations and diseases of the joints has been provided by several clinical and epidemiological studies. Immunological studies have shown the presence of shared inflammatory cells both in the gut and the synovium in spondyloarthropathies. Genetic factors play a crucial role in the pathogenesis of spondyloarthropathies and inflammatory bowel disease. The role of the ubiquitous bacterial flora and pathogenic microorganisms present in the intestinal lumen may induce these joint diseases in patients with inflammatory bowel disease. In this review we will focus on the pathogenesis of spondyloarthropathies and arthralgia in patients suffering from inflammatory bowel disease. Based on preliminary clinical observations in patients with arthralgia and IBD, we put forward the hypothesis that probiotics may be helpful in the management of common extraintestinal manifestations such as arthralgia in patients with ulcerative colitis and Crohn's disease.
Recent Advances in Crohn’s Disease, 1981
Recent Advances in Crohn’s Disease, 1981
Recent Advances in Crohn’s Disease, 1981
Recent Advances in Crohn’s Disease, 1981
Recent Advances in Crohn’s Disease, 1981
Recent Advances in Crohn’s Disease, 1981
Recent Advances in Crohn’s Disease, 1981
The aetiology of Crohn’s disease remains unknown. It has been suggested that genetic predispositi... more The aetiology of Crohn’s disease remains unknown. It has been suggested that genetic predisposition, environmental factors and immunological abnormalities play a role in the pathogenesis of the disease.
Revista española de enfermedades digestivas : organo oficial de la Sociedad Española de Patología Digestiva, 2003
The inflammatory bowel disease is accompanied by cutaneous manifestations in approximately 10% of... more The inflammatory bowel disease is accompanied by cutaneous manifestations in approximately 10% of the cases. Neutrophilic dermatoses are located on the dermis and/or epidermis and are characterised on histological examination by the presence of an infiltrate that consists largely of neutrophils. The prototype of neutrophilic dematoses is Sweetacute;s syndrome; which is rarely associated with Crohńs disease. A 63 year old woman was admitted to hospital with pyrexia, abdominal pain, episcleritis and skin lesions. She presented erythematous lesions on trunk, legs and arms, with tendency towards formation of plaques, nodules and vesicular pustular lesions. Both the colonoscopy and colonic biopsies confirmed the diagnosis of colonic Crohńs disease. Cutaneous biopsies re-vealed an infiltrate consisting mainly of neutrophils. These biopsies, together with clinical details led to the diagnosis of Sweetacute;s syndrome. A methylprednisolone treatment rapidly improved the skin lesions and cli...
Inflammatory Bowel Disease — Diagnostic and Therapeutic Strategies, 2007
Journal of Neuroimmunology, 1997
TNF-α production in whole blood cultures upon stimulation with LPS was determined in 179 individu... more TNF-α production in whole blood cultures upon stimulation with LPS was determined in 179 individuals from 61 families in order to characterise the magnitude of inherited differences in TNF-α production. The three families characterised by highest TNF production showed 7.1±0.3 ng TNF/ml upon culture with 10 ng LPS and 10.2±0.2 ng TMF/ml upon culture with 1000 ng LPS, in contrast
Immunology Letters, 1997
Intracellular antigen processing selects peptides for presentation by human leukocyte antigen (HL... more Intracellular antigen processing selects peptides for presentation by human leukocyte antigen (HLA) class I molecules according to unknown rules. To understand the role of the transporters associated with antigen processing (TAP) in peptide selection for presentation by HLA class I molecules, we undertook a comprehensive analysis of the selectiiity of human transporter in TAP/peptida binding assays. We found a pronounced and dominant effect of the 3 N-terminal and the C-terminal residues on binding of 8 to 1Cmer peptides. Binding data of 394 peptides were used to train artificial neural networks (ANNs) that successfully predicted TAP binding affinltles of 100 random sequence peptides. In ANN-simulated TAP binding experiments on almost 2000 ligands for HtA class I molecules, individual HLA class I alleles varied dramatically wlth respect to TAP affinities of their ligands. Some HLA class I alleles, especially HlA-827, should therefore be particularly efficient in presentation of antigens with low cytosolic abundance.
Immunologic Research, 1991
European Journal of Gastroenterology & Hepatology, 1998
European Journal of Gastroenterology & Hepatology, 1998
It has been suggested that genetic predisposition to cancer may be caused by genetic instability ... more It has been suggested that genetic predisposition to cancer may be caused by genetic instability that can be assessed by studying chromosome instability. It can be evidenced by the analysis of spontaneous chromosome aberrations (CAs) and telomeric associations (TAS). AIM: The objective of this work was to determine the presence of chromosomes instability in patients with inflammatory bowel diseases. MATERIAL AND METHODS: Chromosome studies were performed in 19 untreated patients with inflammatory bowel diseases: 16 with ulcerative colitis (UC) (10 females and 6 males) mean age 42.12 years (range 16-66) and 3 with Crohn's disease (CD) (2 males and 1 female) mean age 42.66 years (range 29-66), and 15 healthy controls (4 females and 11 males) mean age 37.47 years (range 23-50). Extension of the colonic involvement was distal (n=4), left-sided (n=5) and extensive (n=7). Activity disease was scored by a clinical and endoscopic index (DAI) in severe (n=4), moderate (n=7), mild (n=4), and remission (n=l). Peripheral blood lymphocytes were cultured for 72 h at 37°C in F-10 medium supplemented with 15% fetal calf serum and 0.1ml of phytohemagglutinine. CAs and TAS were analyzed on 100 Giemsastained metaphases from each individual. Chromosomes and breakpoints were identified by sequential G-banding. Statistical analysis was carried out with the non parametric Kmskal-Wallis test. RESULTS: Mean frequencies of CAs (X_+ES) were significantly increased in UC (4.62+0.64) and CD (4.00 _+ 1.73) patients compared to controls (0.53 _+ 0.33) (p<0.0003 and p<0.05, respectively). Moreover, the mean frequencies of TAS in UC (10.37 _+ 1,59) and CD patients (10.00 _+ 1.52) were significantly increased in respect to controls (1.26 ± 0.30) (p<0.002). No significant differences were found either between UC and CD patients nor among the different groups of UC patients. In UC patients, short arms were more affected in TAS (52.5%) than long arms and chromosomes 11 (8.12%), 16 (7.19%), 10 (6.25%) and 20 (5.94%) were the most frequently involved. DISCUSSION: Our results indicate that UC and CD patients exhibit chromosome instability involving specific chromosomes and breakpoints, suggesting that it could be related to the cancer predisposition observed in these diseases.
Digestion, 2009
Hereditary alpha 1-antitrypsin (alpha 1-AT) deficiency has been suggested to be associated with p... more Hereditary alpha 1-antitrypsin (alpha 1-AT) deficiency has been suggested to be associated with peptic ulcer disease. Since the serum concentration of the enzyme is the result of both hereditary and nonhereditary factors, we have studied not only the serum levels but also the alpha 1-AT electrophoretic variants in 177 Dutch patients with duodenal ulcer disease and compared with 357 healthy blood donors. No relation was found between any of the alpha 1-antitrypsin phenotypes and duodenal ulcer disease. Serum levels of alpha 1-AT were significantly higher than in the controls in the patients. This study does not support an association between hereditary alpha 1-AT deficiency and duodenal ulcer disease, and makes therefore a possible role of such a deficiency in the etiology of peptic ulcer disease highly unlikely.
Digestion, 1987
To determine whether patients with Crohn&amp;amp;#39;s disease and ulcerative colitis differ ... more To determine whether patients with Crohn&amp;amp;#39;s disease and ulcerative colitis differ in their capacity to produce immunoglobulins, we have studied the in vitro immunoglobulin synthesis by peripheral blood lymphocytes of such patients and compared the results with those of healthy controls. The spontaneous immunoglobulin production by peripheral blood lymphocytes from Crohn&amp;amp;#39;s disease patients was significantly increased for both IgA and IgG, whereas for IgM the increase did not reach statistical significance. In ulcerative colitis, however, the spontaneous IgA and IgG production was almost identical to that of the healthy controls and thus lower than in Crohn&amp;amp;#39;s disease. Pokeweed mitogen stimulation resulted in a significantly enhanced immunoglobulin production in Crohn&amp;amp;#39;s disease and in controls, whereas peripheral blood lymphocytes from ulcerative colitis patients were found to be rather insensitive to pokeweed mitogen stimulation. Suppression of the stimulated immunoglobulin production by concanavalin A revealed considerable reduction in all groups studied. In general, the highest suppression was found in patients with Crohn&amp;amp;#39;s disease. In patients with Crohn&amp;amp;#39;s disease or ulcerative colitis there was no relation between the changes in the in vitro immunoglobulin synthesis and the population of B or T lymphocytes and monocytes present in the peripheral blood. The differences in the spontaneous and stimulated immunoglobulin synthesis by peripheral blood lymphocytes, as found in this study, point to major changes in the regulation of the immunoglobulin synthesis in Crohn&amp;amp;#39;s disease and ulcerative colitis.
Uploads
Papers by Amado Salvador Peña