The objective of this work was to characterize the grape germplasm in Santa Catarina, Brazil, usi... more The objective of this work was to characterize the grape germplasm in Santa Catarina, Brazil, using microsatellite DNA markers (simple sequence repeats -SSR). The DNA samples were collected from leaves and shoots of accessions of public and private collections from the counties Urussanga, Nova Trento, Rodeio, São Joaquim, Campos Novos, Videira, and Água Doce. Ten SSR loci (VVS2, VVMD5, VVMD7, VVMD27, VrZAG62, VrZAG79, VVMD25, VVMD28, VVMD31, and VVMD32) were analysed by capillary electrophoresis. Molecular profiling was conducted for 190 grapevines (European, American, and hybrids), and 67 genotypes were obtained. The data were compared with each other and with those from the literature and from online databases, in order to identify varieties and discover cases of synonymy and homonymy. Forty molecular profiles corresponded to known varieties, while 27 genotypes were described for the first time. The existence of typical germplasm composed mainly of American and hybrid varieties is an important finding for local viticulture. Applications of the results rely on quality control and certification at the nursery level. Increasing precision in the characterization of grapevine genotypes may help breeding programs.
Page 1. Pesq. agropec. bras., Brasília, v. 38, n. 7, p. 783-789, jul. 2003 Propagação in vitro e ... more Page 1. Pesq. agropec. bras., Brasília, v. 38, n. 7, p. 783-789, jul. 2003 Propagação in vitro e avaliação de parâmetros 783 Propagação in vitro e avaliação de parâmetros morfofisiológicos de porta-enxertos de videira(1) Marcelo ...
Cultivars used for wine and table grape have selffertile hermaphrodite flowers whereas wild Europ... more Cultivars used for wine and table grape have selffertile hermaphrodite flowers whereas wild European vines and American and Asian species are dioecious, having either male or female flowers. Consistent with previous studies, the flower sex trait was mapped as a single major locus on chromosome 2 based on a pure Vitis vinifera population segregating for hermaphrodite and female progeny, and a hybrid population producing all three flower sex types. The sex locus was placed between the same SSR and SNP markers on both genetic maps, although abnormal segregation hampered to fine map the genomic region. From a total of 55 possible haplotypes inferred for three SSR markers around the sex locus, in a population of 132 V. sylvestris accessions and 171 V. vinifera cultivars, one of them accounted for 66 % of the hermaphrodite individuals and may be the result of domestication. Specific size variants of the VVIB23 microsatellite sequence within the 3 0 -UTR of a putative YABBY1 gene were found to be statistically significantly associated with the sex alleles M, H and f; these markers can provide assistance in defining the status of wild grapevine germplasm.
Grapevine is one of the world's most important fruit crops. All 60 2n = 38 Vitis species worldwid... more Grapevine is one of the world's most important fruit crops. All 60 2n = 38 Vitis species worldwide are diploids that cross easily; hybrids are fertile and advanced generation pedigrees are available. The cultivated grape species Vitis vinifera has the potential to become a model for fruit tree genetics. Given its cultural and economic importance, grapevine has received much attention from the scientific community in the last few years, resulting in considerable progress in genetic and genomic research. A consensus sequence of the grapevine genome was generated, providing information on overall organization, gene content, and structural components of the DNA in the 19 chromosomes of V. vinifera. Extensive genetic mapping has been conducted in Vitis ssp. based on SSR markers, including the identification of quantitative trait loci for a variety of traits. A large set of single-nucleotide polymorphisms was developed from expressed sequence tags, bacterial artificial chromosomeend sequences, and unique regions of the assembled genome of Pinot noir, providing a comprehensive grapevine genetic map. Single-nucleotide polymorphism markers represent a substantial resource for molecular breeding programs, providing a new basis for map-based gene isolation and fine-mapping quantitative trait loci by identifying candidate genes.
Two populations (Pop) segregating quantitatively for resistance to downy mildew (DM), caused by P... more Two populations (Pop) segregating quantitatively for resistance to downy mildew (DM), caused by Plasmopara viticola, were used to construct genetic maps and to carry out quantitative trait locus (QTL) analysis. Pop1 comprised of 174 F 1 individuals from a cross of 'Moscato Bianco', a susceptible Vitis vinifera cultivar, and a resistant individual of Vitis riparia. Pop2 consisted of 94 progeny from a cross of two interspecific hybrids, 'VRH3082 1-42' and 'SK77 5/3', with resistance traits inherited from Vitis rotundifolia and Vitis amurensis, respectively. Resistance of progeny was measured in field and greenhouse conditions by visual evaluation of disease symptoms on leaves. Linkage maps of 1037.2 and 651 cM were built essentially with simple sequence repeat markers and were enriched with gene-derived single-strand conformational polymorphism and single-nucleotide polymorphism markers. Simple interval mapping and Kruskall-Wallis analysis detected a stable QTL involved in field resistance to DM on linkage group (LG) 7 of the Pop1 integrated map co-localized with a putative Caffeoyl-CoA O-methyltransferase-derived marker. Additional QTLs were detected on LGs 8, 12 and 17. We were able to identify genetic factors correlated with resistance to P. viticola with lower statistical significance on
The objective of this work was to characterize the grape germplasm in Santa Catarina, Brazil, usi... more The objective of this work was to characterize the grape germplasm in Santa Catarina, Brazil, using microsatellite DNA markers (simple sequence repeats -SSR). The DNA samples were collected from leaves and shoots of accessions of public and private collections from the counties Urussanga, Nova Trento, Rodeio, São Joaquim, Campos Novos, Videira, and Água Doce. Ten SSR loci (VVS2, VVMD5, VVMD7, VVMD27, VrZAG62, VrZAG79, VVMD25, VVMD28, VVMD31, and VVMD32) were analysed by capillary electrophoresis. Molecular profiling was conducted for 190 grapevines (European, American, and hybrids), and 67 genotypes were obtained. The data were compared with each other and with those from the literature and from online databases, in order to identify varieties and discover cases of synonymy and homonymy. Forty molecular profiles corresponded to known varieties, while 27 genotypes were described for the first time. The existence of typical germplasm composed mainly of American and hybrid varieties is an important finding for local viticulture. Applications of the results rely on quality control and certification at the nursery level. Increasing precision in the characterization of grapevine genotypes may help breeding programs.
Page 1. Pesq. agropec. bras., Brasília, v. 38, n. 7, p. 783-789, jul. 2003 Propagação in vitro e ... more Page 1. Pesq. agropec. bras., Brasília, v. 38, n. 7, p. 783-789, jul. 2003 Propagação in vitro e avaliação de parâmetros 783 Propagação in vitro e avaliação de parâmetros morfofisiológicos de porta-enxertos de videira(1) Marcelo ...
Cultivars used for wine and table grape have selffertile hermaphrodite flowers whereas wild Europ... more Cultivars used for wine and table grape have selffertile hermaphrodite flowers whereas wild European vines and American and Asian species are dioecious, having either male or female flowers. Consistent with previous studies, the flower sex trait was mapped as a single major locus on chromosome 2 based on a pure Vitis vinifera population segregating for hermaphrodite and female progeny, and a hybrid population producing all three flower sex types. The sex locus was placed between the same SSR and SNP markers on both genetic maps, although abnormal segregation hampered to fine map the genomic region. From a total of 55 possible haplotypes inferred for three SSR markers around the sex locus, in a population of 132 V. sylvestris accessions and 171 V. vinifera cultivars, one of them accounted for 66 % of the hermaphrodite individuals and may be the result of domestication. Specific size variants of the VVIB23 microsatellite sequence within the 3 0 -UTR of a putative YABBY1 gene were found to be statistically significantly associated with the sex alleles M, H and f; these markers can provide assistance in defining the status of wild grapevine germplasm.
Grapevine is one of the world's most important fruit crops. All 60 2n = 38 Vitis species worldwid... more Grapevine is one of the world's most important fruit crops. All 60 2n = 38 Vitis species worldwide are diploids that cross easily; hybrids are fertile and advanced generation pedigrees are available. The cultivated grape species Vitis vinifera has the potential to become a model for fruit tree genetics. Given its cultural and economic importance, grapevine has received much attention from the scientific community in the last few years, resulting in considerable progress in genetic and genomic research. A consensus sequence of the grapevine genome was generated, providing information on overall organization, gene content, and structural components of the DNA in the 19 chromosomes of V. vinifera. Extensive genetic mapping has been conducted in Vitis ssp. based on SSR markers, including the identification of quantitative trait loci for a variety of traits. A large set of single-nucleotide polymorphisms was developed from expressed sequence tags, bacterial artificial chromosomeend sequences, and unique regions of the assembled genome of Pinot noir, providing a comprehensive grapevine genetic map. Single-nucleotide polymorphism markers represent a substantial resource for molecular breeding programs, providing a new basis for map-based gene isolation and fine-mapping quantitative trait loci by identifying candidate genes.
Two populations (Pop) segregating quantitatively for resistance to downy mildew (DM), caused by P... more Two populations (Pop) segregating quantitatively for resistance to downy mildew (DM), caused by Plasmopara viticola, were used to construct genetic maps and to carry out quantitative trait locus (QTL) analysis. Pop1 comprised of 174 F 1 individuals from a cross of 'Moscato Bianco', a susceptible Vitis vinifera cultivar, and a resistant individual of Vitis riparia. Pop2 consisted of 94 progeny from a cross of two interspecific hybrids, 'VRH3082 1-42' and 'SK77 5/3', with resistance traits inherited from Vitis rotundifolia and Vitis amurensis, respectively. Resistance of progeny was measured in field and greenhouse conditions by visual evaluation of disease symptoms on leaves. Linkage maps of 1037.2 and 651 cM were built essentially with simple sequence repeat markers and were enriched with gene-derived single-strand conformational polymorphism and single-nucleotide polymorphism markers. Simple interval mapping and Kruskall-Wallis analysis detected a stable QTL involved in field resistance to DM on linkage group (LG) 7 of the Pop1 integrated map co-localized with a putative Caffeoyl-CoA O-methyltransferase-derived marker. Additional QTLs were detected on LGs 8, 12 and 17. We were able to identify genetic factors correlated with resistance to P. viticola with lower statistical significance on
Uploads
Papers by Flavia Moreira