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T. Abedi

Hiroshima University, Advanced Sciences of Matter, Graduate Student

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Jordan Sinclair

Rice University

Isfahan University of Technology

Amru Nasrulhaq Boyce

University of Malaya, Malaysia

Ajar Nath Yadav

INTI Interntional University

Tomonori Kawano

The University of Kitakyushu

Alice Gilman-Sachs

Sanika Naik Samant

Goa University, India

Indian Council of Agricultural Research (ICAR)

Ghassan J A A F A R Hamdi

University of Diyala

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Papers by T. Abedi

Antioxidant enzymes changes in response to drought stress in ten cultivars of oilseed rape (Brassica napus L.)

Czech Journal of Genetics and Plant Breeding, 2010

The study was undertaken to identify the responses of antioxidant enzyme activities and their iso... more The study was undertaken to identify the responses of antioxidant enzyme activities and their isozyme patterns in seedlings of 10 oilseed rape (Brassica napus L.) cultivars under drought stress conditions. Plants were grown under three irrigation regimes (FC; field capacity, 60% FC and 30% FC) in a greenhouse. Drought stress preferentially enhanced the activities of superoxide dismutase (SOD) and guaiacol peroxidase (POD) whereas it decreased catalase (CAT) activity. Licord with the highest level of enzyme activity under both optimum and limited irrigation regimes is reported as the most tolerant cultivar. Whereas Hyola 308 and Okapy, having the lowest enzymes activities, are mentioned as cultivars sensitive to drought stress. The native polyacrylamide gel electrophoresis (PAGE) analysis detected eight SOD isozymes. Oilseed rape leaves contained three isoforms of Mn-SOD and five isoforms of Cu/Zn-SOD. The expression of Mn-SOD was preferentially enhanced by drought stress. Five POD i...

Wheat yield and grain protein response to nitrogen amount and timing

cropj.com

This research was carried out to study the effect of nitrogen rate application (0, 120, 240 and 3... more This research was carried out to study the effect of nitrogen rate application (0, 120, 240 and 360 kg Nitrogen ha -1 ) and nitrogen timing on grain yield, yield components, grain quality and protein banding pattern in different growth stages of wheat (Shiraz cultivar) which was grown at research station of the School of Agriculture, Shiraz University at Bajgah in the 2008-2009. Results indicate that the highest value for grain yield was obtained at 240 kg N ha -1 when it was applied through vegetative growth stages (8230 kg ha -1 ). Yield components were significantly increased with enhancing the level of nitrogen with no significant difference between 240 and 360 kg N ha -1 . Results show that no N application at tillering stage decreased spikes number/m 2 and seeds spike -1 , and the minimum value of 1000 grain weight was observed when we remove N application in grain filling period. Leaf protein did not influenced by N treatments. However, the sharpness of a band with molecular weigh about 51 kDa, related to Rubisco enzyme, was increased in some treatments. Furthermore, 240 kg N ha -1 application in all timing treatments resulted in maximum seed protein content (5.5 mg protein g -1 grain). At the same time, seed water soluble proteins, Albumin and Globulin, showed no polymorphism. While different N rate treatments had no significant effect on seed gluten content, no N application in grain filling period declined it significantly. All in all, obtaining high wheat grain yield beside a suitable bakery quality are possible in a proper farming management system without environmental impact of N over application. These goals may be achieved with sufficient N application during vegetative growth for yield and late season N application for protein quality.

UDP-galactose transporter gene hUGT1 expression in tobacco plants leads to hyper-galactosylated cell wall components

Journal of bioscience and bioengineering, Jan 24, 2015

We reported previously that tobacco plants transformed with the human UDP-galactose transporter 1... more We reported previously that tobacco plants transformed with the human UDP-galactose transporter 1 gene (hUGT1-transgenic plants) displayed morphological, architectural, and physiological alterations, such as enhanced growth, increased accumulation of chlorophyll and lignin, and a gibberellin-responsive phenotype. In the present study, we demonstrated that hUGT1 expression altered the monosaccharide composition of cell wall matrix polysaccharides, such as pectic and hemicellulosic polysaccharides, which are biosynthesized in the Golgi lumen. An analysis of the monosaccharide composition of the cell wall matrix polysaccharides revealed that the ratio of galactose to total monosaccharides was significantly elevated in the hemicellulose II and pectin fractions of hUGT1-transgenic plants compared with that of control plants. A hyper-galactosylated xyloglucan structure was detected in hemicellulose II using oligosaccharide mass profiling. These results indicated that, because of the enhan...

UDP-galactose transporter gene hUGT1 expression in tobacco plants leads to hyper-galactosylated cell wall components

We reported previously that tobacco plants transformed with the human UDP-galactose transporter 1... more We reported previously that tobacco plants transformed with the human UDP-galactose transporter 1 gene (hUGT1-transgenic plants) displayed morphological, architectural, and physiological alterations, such as enhanced growth, increased accumulation of chlorophyll and lignin, and a gibberellin-responsive phenotype. In the present study, we demonstrated that hUGT1 expression altered the monosaccharide composition of cell wall matrix polysaccharides, such as pectic and hemicellulosic polysaccharides, which are biosynthesized in the Golgi lumen. An analysis of the mono-saccharide composition of the cell wall matrix polysaccharides revealed that the ratio of galactose to total mono-saccharides was significantly elevated in the hemicellulose II and pectin fractions of hUGT1-transgenic plants compared with that of control plants. A hyper-galactosylated xyloglucan structure was detected in hemicellulose II using oligo-saccharide mass profiling. These results indicated that, because of the enhanced UDP-galactose transport from the cytosol to the Golgi apparatus by hUGT1, galactose incorporation in the cell wall matrix polysaccharides increased. This increased galactose incorporation may have contributed to increased galactose tolerance in hUGT1-transgenic plants.

Wheat yield and grain protein response to nitrogen amount and timing

This research was carried out to study the effect of nitrogen rate application (0, 120, 240 and 3... more This research was carried out to study the effect of nitrogen rate application (0, 120, 240 and 360 kg Nitrogen ha-1) and nitrogen timing on grain yield, yield components, grain quality and protein banding pattern in different growth stages of wheat (Shiraz cultivar) which was grown at research station of the School of Agriculture, Shiraz University at Bajgah in the 2008-2009. Results indicate that the highest value for grain yield was obtained at 240 kg N ha −1 when it was applied through vegetative growth stages (8230 kg ha-1). Yield components were significantly increased with enhancing the level of nitrogen with no significant difference between 240 and 360 kg N ha-1. Results show that no N application at tillering stage decreased spikes number/m 2 and seeds spike-1 , and the minimum value of 1000 grain weight was observed when we remove N application in grain filling period. Leaf protein did not influenced by N treatments. However, the sharpness of a band with molecular weigh about 51 kDa, related to Rubisco enzyme, was increased in some treatments. Furthermore, 240 kg N ha-1 application in all timing treatments resulted in maximum seed protein content (5.5 mg protein g-1 grain). At the same time, seed water soluble proteins, Albumin and Globulin, showed no polymorphism. While different N rate treatments had no significant effect on seed gluten content, no N application in grain filling period declined it significantly. All in all, obtaining high wheat grain yield beside a suitable bakery quality are possible in a proper farming management system without environmental impact of N over application. These goals may be achieved with sufficient N application during vegetative growth for yield and late season N application for protein quality.

Antioxidant Enzyme Changes in Response to Drought Stress in Ten Cultivars of Oilseed Rape (Brassica napus L

by T. Abedi and Hassan Pakniyat

The study was undertaken to identify the responses of antioxidant enzyme activities and their iso... more The study was undertaken to identify the responses of antioxidant enzyme activities and their isozyme patterns in seedlings of 10 oilseed rape (Brassica napus L.) cultivars under drought stress conditions. Plants were grown under three irrigation regimes (FC; field capacity, 60% FC and 30% FC) in a greenhouse. Drought stress preferentially enhanced the activities of superoxide dismutase (SOD) and guaiacol peroxidase (POD) whereas it decreased catalase (CAT) activity. Licord with the highest level of enzyme activity under both optimum and limited irrigation regimes is reported as the most tolerant cultivar. Whereas Hyola 308 and Okapy, having the lowest enzymes activities, are mentioned as cultivars sensitive to drought stress. The native polyacrylamide gel electrophoresis (PAGE) analysis detected eight SOD isozymes. Oilseed rape leaves contained three isoforms of Mn-SOD and five isoforms of Cu/Zn-SOD. The expression of Mn-SOD was preferentially enhanced by drought stress. Five POD isoforms were detected in oilseed rape leaves. The intensities of POD-4 and-5 were enhanced under drought stress. According to the results, the appearance of new isozyme bands under drought stress conditions may be used as a biochemical marker to differentiate drought tolerant cultivars under drought stress.

Effect of organic and inorganic fertilizers on grain yield and protein banding pattern of wheat

In order to evaluate the effects of different levels of inorganic (0, 80, 160 and 240 kg Nitrogen... more In order to evaluate the effects of different levels of inorganic (0, 80, 160 and 240 kg Nitrogen ha-1) and organic (0, 30 and 60 Mg municipal waste compost ha-1) fertilizers on wheat grain yield, gluten content, protein variability and protein banding pattern on polyacrylamide gel in different growth stages of irrigated wheat, a field experiment was conducted at research station of the School of Agriculture, Shiraz University at Bajgah in 2007. Results indicated that the highest wheat grain yield was achieved when the plants were fertilized with 160 kg N ha-1 and 30 Mg compost ha-1. Among yield components, spikes plant-1 , seeds spike-1 and 1000 kernels weight were significantly increased with increasing the level of nitrogen. However, there was no significant difference between 160 and 240 kg N ha-1. There was no significant effect of N fertilization on seed protein and gluten content, however the highest amount of seed protein was obtained in 60 Mg compost ha-1 at all levels of nitrogen. The SDS-PAGE was performed to investigate differences between proteins banding pattern in different growth stages under different levels of N and compost. Protein banding pattern showed no polymorphism in tillering and stem elongation stages. However, in ear emergence in 160 kg N ha-1 and in the all compost levels, density of a 50 kDa band was increased specifically in 60 Mg compost ha-1. Furthermore, seed water soluble proteins, Albumin and Globulin, showed no polymorphism. All in all, it is possible to obtain maximum grain yield, protein and gluten, just in 160 kg ha-1 nitrogen level. Thus, it shows the positive impact of compost application on reduction of chemical fertilizer use.

Antioxidant Enzyme Changes in Response to Drought Stress in Ten Cultivars of Oilseed Rape (Brassica napus L.)

by Hassan Pakniyat and T. Abedi

The study was undertaken to identify the responses of antioxidant enzyme activities and their iso... more The study was undertaken to identify the responses of antioxidant enzyme activities and their isozyme patterns in seedlings of 10 oilseed rape (Brassica napus L.) cultivars under drought stress conditions. Plants were grown under three irrigation regimes (FC; field capacity, 60% FC and 30% FC) in a greenhouse. Drought stress preferentially enhanced the activities of superoxide dismutase (SOD) and guaiacol peroxidase (POD) whereas it decreased catalase (CAT) activity. Licord with the highest level of enzyme activity under both optimum and limited irrigation regimes is reported as the most tolerant cultivar. Whereas Hyola 308 and Okapy, having the lowest enzymes activities, are mentioned as cultivars sensitive to drought stress. The native polyacrylamide gel electrophoresis (PAGE) analysis detected eight SOD isozymes. Oilseed rape leaves contained three isoforms of Mn-SOD and five isoforms of Cu/Zn-SOD. The expression of Mn-SOD was preferentially enhanced by drought stress. Five POD isoforms were detected in oilseed rape leaves. The intensities of POD-4 and -5 were enhanced under drought stress. According to the results, the appearance of new isozyme bands under drought stress conditions may be used as a biochemical marker to differentiate drought tolerant cultivars under drought stress.

Antioxidant enzyme changes in response to drought stress in ten cultivars of Oilseed Rape (Brassica napus L.)

by T. Abedi and Hassan Pakniyat

The study was undertaken to identify the responses of antioxidant enzyme activities and their iso... more The study was undertaken to identify the responses of antioxidant enzyme activities and their isozyme patterns in seedlings of 10 oilseed rape (Brassica napus L.) cultivars under drought stress conditions. Plants were grown under three irrigation regimes (FC; field capacity, 60% FC and 30% FC) in a greenhouse. Drought stress preferentially enhanced the activities of superoxide dismutase (SOD) and guaiacol peroxidase (POD) whereas it decreased catalase (CAT) activity. Licord with the highest level of enzyme activity under both optimum and limited irrigation regimes is reported as the most tolerant cultivar. Whereas Hyola 308 and Okapy, having the lowest enzymes activities, are mentioned as cultivars sensitive to drought stress. The native polyacrylamide gel electrophoresis (PAGE) analysis detected eight SOD isozymes. Oilseed rape leaves contained three isoforms of Mn-SOD and five isoforms of Cu/Zn-SOD. The expression of Mn-SOD was preferentially enhanced by drought stress. Five POD isoforms were detected in oilseed rape leaves. The intensities of POD-4 and -5 were enhanced under drought stress. According to the results, the appearance of new isozyme bands under drought stress conditions may be used as a biochemical marker to differentiate drought tolerant cultivars under drought stress.

Antioxidant enzymes changes in response to drought stress in ten cultivars of oilseed rape (Brassica napus L.)

Czech Journal of Genetics and Plant Breeding, 2010

The study was undertaken to identify the responses of antioxidant enzyme activities and their iso... more The study was undertaken to identify the responses of antioxidant enzyme activities and their isozyme patterns in seedlings of 10 oilseed rape (Brassica napus L.) cultivars under drought stress conditions. Plants were grown under three irrigation regimes (FC; field capacity, 60% FC and 30% FC) in a greenhouse. Drought stress preferentially enhanced the activities of superoxide dismutase (SOD) and guaiacol peroxidase (POD) whereas it decreased catalase (CAT) activity. Licord with the highest level of enzyme activity under both optimum and limited irrigation regimes is reported as the most tolerant cultivar. Whereas Hyola 308 and Okapy, having the lowest enzymes activities, are mentioned as cultivars sensitive to drought stress. The native polyacrylamide gel electrophoresis (PAGE) analysis detected eight SOD isozymes. Oilseed rape leaves contained three isoforms of Mn-SOD and five isoforms of Cu/Zn-SOD. The expression of Mn-SOD was preferentially enhanced by drought stress. Five POD i...

Wheat yield and grain protein response to nitrogen amount and timing

cropj.com

This research was carried out to study the effect of nitrogen rate application (0, 120, 240 and 3... more This research was carried out to study the effect of nitrogen rate application (0, 120, 240 and 360 kg Nitrogen ha -1 ) and nitrogen timing on grain yield, yield components, grain quality and protein banding pattern in different growth stages of wheat (Shiraz cultivar) which was grown at research station of the School of Agriculture, Shiraz University at Bajgah in the 2008-2009. Results indicate that the highest value for grain yield was obtained at 240 kg N ha -1 when it was applied through vegetative growth stages (8230 kg ha -1 ). Yield components were significantly increased with enhancing the level of nitrogen with no significant difference between 240 and 360 kg N ha -1 . Results show that no N application at tillering stage decreased spikes number/m 2 and seeds spike -1 , and the minimum value of 1000 grain weight was observed when we remove N application in grain filling period. Leaf protein did not influenced by N treatments. However, the sharpness of a band with molecular weigh about 51 kDa, related to Rubisco enzyme, was increased in some treatments. Furthermore, 240 kg N ha -1 application in all timing treatments resulted in maximum seed protein content (5.5 mg protein g -1 grain). At the same time, seed water soluble proteins, Albumin and Globulin, showed no polymorphism. While different N rate treatments had no significant effect on seed gluten content, no N application in grain filling period declined it significantly. All in all, obtaining high wheat grain yield beside a suitable bakery quality are possible in a proper farming management system without environmental impact of N over application. These goals may be achieved with sufficient N application during vegetative growth for yield and late season N application for protein quality.

UDP-galactose transporter gene hUGT1 expression in tobacco plants leads to hyper-galactosylated cell wall components

Journal of bioscience and bioengineering, Jan 24, 2015

We reported previously that tobacco plants transformed with the human UDP-galactose transporter 1... more We reported previously that tobacco plants transformed with the human UDP-galactose transporter 1 gene (hUGT1-transgenic plants) displayed morphological, architectural, and physiological alterations, such as enhanced growth, increased accumulation of chlorophyll and lignin, and a gibberellin-responsive phenotype. In the present study, we demonstrated that hUGT1 expression altered the monosaccharide composition of cell wall matrix polysaccharides, such as pectic and hemicellulosic polysaccharides, which are biosynthesized in the Golgi lumen. An analysis of the monosaccharide composition of the cell wall matrix polysaccharides revealed that the ratio of galactose to total monosaccharides was significantly elevated in the hemicellulose II and pectin fractions of hUGT1-transgenic plants compared with that of control plants. A hyper-galactosylated xyloglucan structure was detected in hemicellulose II using oligosaccharide mass profiling. These results indicated that, because of the enhan...

UDP-galactose transporter gene hUGT1 expression in tobacco plants leads to hyper-galactosylated cell wall components

We reported previously that tobacco plants transformed with the human UDP-galactose transporter 1... more We reported previously that tobacco plants transformed with the human UDP-galactose transporter 1 gene (hUGT1-transgenic plants) displayed morphological, architectural, and physiological alterations, such as enhanced growth, increased accumulation of chlorophyll and lignin, and a gibberellin-responsive phenotype. In the present study, we demonstrated that hUGT1 expression altered the monosaccharide composition of cell wall matrix polysaccharides, such as pectic and hemicellulosic polysaccharides, which are biosynthesized in the Golgi lumen. An analysis of the mono-saccharide composition of the cell wall matrix polysaccharides revealed that the ratio of galactose to total mono-saccharides was significantly elevated in the hemicellulose II and pectin fractions of hUGT1-transgenic plants compared with that of control plants. A hyper-galactosylated xyloglucan structure was detected in hemicellulose II using oligo-saccharide mass profiling. These results indicated that, because of the enhanced UDP-galactose transport from the cytosol to the Golgi apparatus by hUGT1, galactose incorporation in the cell wall matrix polysaccharides increased. This increased galactose incorporation may have contributed to increased galactose tolerance in hUGT1-transgenic plants.

Wheat yield and grain protein response to nitrogen amount and timing

This research was carried out to study the effect of nitrogen rate application (0, 120, 240 and 3... more This research was carried out to study the effect of nitrogen rate application (0, 120, 240 and 360 kg Nitrogen ha-1) and nitrogen timing on grain yield, yield components, grain quality and protein banding pattern in different growth stages of wheat (Shiraz cultivar) which was grown at research station of the School of Agriculture, Shiraz University at Bajgah in the 2008-2009. Results indicate that the highest value for grain yield was obtained at 240 kg N ha −1 when it was applied through vegetative growth stages (8230 kg ha-1). Yield components were significantly increased with enhancing the level of nitrogen with no significant difference between 240 and 360 kg N ha-1. Results show that no N application at tillering stage decreased spikes number/m 2 and seeds spike-1 , and the minimum value of 1000 grain weight was observed when we remove N application in grain filling period. Leaf protein did not influenced by N treatments. However, the sharpness of a band with molecular weigh about 51 kDa, related to Rubisco enzyme, was increased in some treatments. Furthermore, 240 kg N ha-1 application in all timing treatments resulted in maximum seed protein content (5.5 mg protein g-1 grain). At the same time, seed water soluble proteins, Albumin and Globulin, showed no polymorphism. While different N rate treatments had no significant effect on seed gluten content, no N application in grain filling period declined it significantly. All in all, obtaining high wheat grain yield beside a suitable bakery quality are possible in a proper farming management system without environmental impact of N over application. These goals may be achieved with sufficient N application during vegetative growth for yield and late season N application for protein quality.

Antioxidant Enzyme Changes in Response to Drought Stress in Ten Cultivars of Oilseed Rape (Brassica napus L

by T. Abedi and Hassan Pakniyat

The study was undertaken to identify the responses of antioxidant enzyme activities and their iso... more The study was undertaken to identify the responses of antioxidant enzyme activities and their isozyme patterns in seedlings of 10 oilseed rape (Brassica napus L.) cultivars under drought stress conditions. Plants were grown under three irrigation regimes (FC; field capacity, 60% FC and 30% FC) in a greenhouse. Drought stress preferentially enhanced the activities of superoxide dismutase (SOD) and guaiacol peroxidase (POD) whereas it decreased catalase (CAT) activity. Licord with the highest level of enzyme activity under both optimum and limited irrigation regimes is reported as the most tolerant cultivar. Whereas Hyola 308 and Okapy, having the lowest enzymes activities, are mentioned as cultivars sensitive to drought stress. The native polyacrylamide gel electrophoresis (PAGE) analysis detected eight SOD isozymes. Oilseed rape leaves contained three isoforms of Mn-SOD and five isoforms of Cu/Zn-SOD. The expression of Mn-SOD was preferentially enhanced by drought stress. Five POD isoforms were detected in oilseed rape leaves. The intensities of POD-4 and-5 were enhanced under drought stress. According to the results, the appearance of new isozyme bands under drought stress conditions may be used as a biochemical marker to differentiate drought tolerant cultivars under drought stress.

Effect of organic and inorganic fertilizers on grain yield and protein banding pattern of wheat

In order to evaluate the effects of different levels of inorganic (0, 80, 160 and 240 kg Nitrogen... more In order to evaluate the effects of different levels of inorganic (0, 80, 160 and 240 kg Nitrogen ha-1) and organic (0, 30 and 60 Mg municipal waste compost ha-1) fertilizers on wheat grain yield, gluten content, protein variability and protein banding pattern on polyacrylamide gel in different growth stages of irrigated wheat, a field experiment was conducted at research station of the School of Agriculture, Shiraz University at Bajgah in 2007. Results indicated that the highest wheat grain yield was achieved when the plants were fertilized with 160 kg N ha-1 and 30 Mg compost ha-1. Among yield components, spikes plant-1 , seeds spike-1 and 1000 kernels weight were significantly increased with increasing the level of nitrogen. However, there was no significant difference between 160 and 240 kg N ha-1. There was no significant effect of N fertilization on seed protein and gluten content, however the highest amount of seed protein was obtained in 60 Mg compost ha-1 at all levels of nitrogen. The SDS-PAGE was performed to investigate differences between proteins banding pattern in different growth stages under different levels of N and compost. Protein banding pattern showed no polymorphism in tillering and stem elongation stages. However, in ear emergence in 160 kg N ha-1 and in the all compost levels, density of a 50 kDa band was increased specifically in 60 Mg compost ha-1. Furthermore, seed water soluble proteins, Albumin and Globulin, showed no polymorphism. All in all, it is possible to obtain maximum grain yield, protein and gluten, just in 160 kg ha-1 nitrogen level. Thus, it shows the positive impact of compost application on reduction of chemical fertilizer use.

Antioxidant Enzyme Changes in Response to Drought Stress in Ten Cultivars of Oilseed Rape (Brassica napus L.)

by Hassan Pakniyat and T. Abedi

The study was undertaken to identify the responses of antioxidant enzyme activities and their iso... more The study was undertaken to identify the responses of antioxidant enzyme activities and their isozyme patterns in seedlings of 10 oilseed rape (Brassica napus L.) cultivars under drought stress conditions. Plants were grown under three irrigation regimes (FC; field capacity, 60% FC and 30% FC) in a greenhouse. Drought stress preferentially enhanced the activities of superoxide dismutase (SOD) and guaiacol peroxidase (POD) whereas it decreased catalase (CAT) activity. Licord with the highest level of enzyme activity under both optimum and limited irrigation regimes is reported as the most tolerant cultivar. Whereas Hyola 308 and Okapy, having the lowest enzymes activities, are mentioned as cultivars sensitive to drought stress. The native polyacrylamide gel electrophoresis (PAGE) analysis detected eight SOD isozymes. Oilseed rape leaves contained three isoforms of Mn-SOD and five isoforms of Cu/Zn-SOD. The expression of Mn-SOD was preferentially enhanced by drought stress. Five POD isoforms were detected in oilseed rape leaves. The intensities of POD-4 and -5 were enhanced under drought stress. According to the results, the appearance of new isozyme bands under drought stress conditions may be used as a biochemical marker to differentiate drought tolerant cultivars under drought stress.

Antioxidant enzyme changes in response to drought stress in ten cultivars of Oilseed Rape (Brassica napus L.)

by T. Abedi and Hassan Pakniyat

The study was undertaken to identify the responses of antioxidant enzyme activities and their iso... more The study was undertaken to identify the responses of antioxidant enzyme activities and their isozyme patterns in seedlings of 10 oilseed rape (Brassica napus L.) cultivars under drought stress conditions. Plants were grown under three irrigation regimes (FC; field capacity, 60% FC and 30% FC) in a greenhouse. Drought stress preferentially enhanced the activities of superoxide dismutase (SOD) and guaiacol peroxidase (POD) whereas it decreased catalase (CAT) activity. Licord with the highest level of enzyme activity under both optimum and limited irrigation regimes is reported as the most tolerant cultivar. Whereas Hyola 308 and Okapy, having the lowest enzymes activities, are mentioned as cultivars sensitive to drought stress. The native polyacrylamide gel electrophoresis (PAGE) analysis detected eight SOD isozymes. Oilseed rape leaves contained three isoforms of Mn-SOD and five isoforms of Cu/Zn-SOD. The expression of Mn-SOD was preferentially enhanced by drought stress. Five POD isoforms were detected in oilseed rape leaves. The intensities of POD-4 and -5 were enhanced under drought stress. According to the results, the appearance of new isozyme bands under drought stress conditions may be used as a biochemical marker to differentiate drought tolerant cultivars under drought stress.