Abstract Homophilic interaction of the L1 family of cell adhesion molecules plays a pivotal role ... more Abstract Homophilic interaction of the L1 family of cell adhesion molecules plays a pivotal role in regulating neurite outgrowth and neural cell networking in vivo. Functional defects in L1 family members are associated with neurological disorders such as X-linked mental retardation, multiple sclerosis, low-IQ syndrome, developmental delay, and schizophrenia.
Abstract: When testing and calibrating particle detectors in a test beam, accurate tracking infor... more Abstract: When testing and calibrating particle detectors in a test beam, accurate tracking information independent of the detector being tested is extremely useful during offline analysis of the data. A general purpose Silicon Beam Tracker (SBT) was constructed with an active area of 32.0 x 32.0 mm2 to provide this capability for the beam calibration of the Cosmic Ray Energetics And Mass (CREAM) calorimeter. The tracker consists of two modules, each comprised of two orthogonal layers of 380 μm thick silicon strip sensors.
Mitogen-activated protein kinase phosphatase 4 (MKP4) has proved to be a promising target for the... more Mitogen-activated protein kinase phosphatase 4 (MKP4) has proved to be a promising target for the development of therapeutics for the treatment of diabetes and the other metabolic diseases. Here, we report an example for a successful application of the structure-based virtual screening to identify three novel inhibitors of MKP4. These inhibitors have desirable physicochemical properties as a drug candidate and reveal a moderate potency with IC50 values ranging from 4.9 to 32.3 µM.
Abstract-Antiallergic activities of two variants of Artemisia princeps Pampanini SJ-1 (named as S... more Abstract-Antiallergic activities of two variants of Artemisia princeps Pampanini SJ-1 (named as Sajabalssuk) and SS~ l (named as Sajuarissuk) cultivated in Ganghwado, which contain high content of eupatilin compared to those cultured by other places, were investigated to evaluate the possibility as inhibitors against allergic diseases. Ethanol and supercritical fluid extracts of SJ-l and SS-l inhibited the release of B-hexosaminidase from RBL-2H3 cells, although their water extracts were inactive.
Abstract The high affinity binding site for human immunodeficiency virus (HIV) envelope glycoprot... more Abstract The high affinity binding site for human immunodeficiency virus (HIV) envelope glycoprotein gp120 resides within the amino-terminal domain (D1) of CD4. Mutational and antibody epitope analyses have implicated the region encompassing residues 40-60 in D1 as the primary binding site for gp120. Outside of this region, a single residue substitution at position 87 abrogates syncytium formation without affecting gp120 binding.
Together with protein tyrosine kinases (PTKs), protein tyrosine phosphatases (PTPs) serve as hall... more Together with protein tyrosine kinases (PTKs), protein tyrosine phosphatases (PTPs) serve as hallmarks in cellular signal transduction by controlling the reversible phosphorylation of their substrates. The human genome is estimated to encode more than 100 PTPs, which can be divided into eleven sub-groups according to their structural and functional characteristics.
Matrix metalloproteinases (MMPs), a family of zinc-containing endopeptidases, participate in many... more Matrix metalloproteinases (MMPs), a family of zinc-containing endopeptidases, participate in many normal processes such as embryonic development and wound repair, and in many pathological situations such as cancer, atherosclerosis, and arthritis. Peptidomimetic MMP inhibitors were designed and synthesized with N-formylhydroxylamine (retrohydroxamate) as a zinc-binding group and various side chains on the α, P1', and P2'positions. Using in vitro MMP assays with purified MMPs (MMP-1, MMP-2, MMP-3, MMP-9, and MMP-14) and ...
Protein kinases are a diverse group of enzymes that are involved in fundamental cellular mechanis... more Protein kinases are a diverse group of enzymes that are involved in fundamental cellular mechanisms such as sensing and signaling in a variety of environmental or intracellular cues, controlling of gene expression and playing crucial role in various metabolisms. To initiate functional studies, we are constructing a bacterial kinase library. Five target kinases related to the development of antimicrobial agents or diagnostic targets and the metabolic engineering were investigated through the various public databases. Three hundred and ...
Anthraquinones have been reported as phosphatase inhibitors. Therefore, anthraquinone derivatives... more Anthraquinones have been reported as phosphatase inhibitors. Therefore, anthraquinone derivatives were screened to identify a potent phosphatase inhibitor against the phosphatase of regenerating liver-3 (PRL-3). Emodin strongly inhibited phosphatase activity of PRL-3 with IC(50) values of 3.5μM and blocked PRL-3-induced tumor cell migration and invasion in a dose-dependent manner. Emodin rescued the phosphorylation of ezrin, which is a known PRL-3 substrate. The results of this study reveal that emodin is a PRL-3 inhibitor and a good lead molecule for obtaining a selective PRL-3 inhibitor.
Abstract Homophilic interaction of the L1 family of cell adhesion molecules plays a pivotal role ... more Abstract Homophilic interaction of the L1 family of cell adhesion molecules plays a pivotal role in regulating neurite outgrowth and neural cell networking in vivo. Functional defects in L1 family members are associated with neurological disorders such as X-linked mental retardation, multiple sclerosis, low-IQ syndrome, developmental delay, and schizophrenia.
Abstract: When testing and calibrating particle detectors in a test beam, accurate tracking infor... more Abstract: When testing and calibrating particle detectors in a test beam, accurate tracking information independent of the detector being tested is extremely useful during offline analysis of the data. A general purpose Silicon Beam Tracker (SBT) was constructed with an active area of 32.0 x 32.0 mm2 to provide this capability for the beam calibration of the Cosmic Ray Energetics And Mass (CREAM) calorimeter. The tracker consists of two modules, each comprised of two orthogonal layers of 380 μm thick silicon strip sensors.
Mitogen-activated protein kinase phosphatase 4 (MKP4) has proved to be a promising target for the... more Mitogen-activated protein kinase phosphatase 4 (MKP4) has proved to be a promising target for the development of therapeutics for the treatment of diabetes and the other metabolic diseases. Here, we report an example for a successful application of the structure-based virtual screening to identify three novel inhibitors of MKP4. These inhibitors have desirable physicochemical properties as a drug candidate and reveal a moderate potency with IC50 values ranging from 4.9 to 32.3 µM.
Abstract-Antiallergic activities of two variants of Artemisia princeps Pampanini SJ-1 (named as S... more Abstract-Antiallergic activities of two variants of Artemisia princeps Pampanini SJ-1 (named as Sajabalssuk) and SS~ l (named as Sajuarissuk) cultivated in Ganghwado, which contain high content of eupatilin compared to those cultured by other places, were investigated to evaluate the possibility as inhibitors against allergic diseases. Ethanol and supercritical fluid extracts of SJ-l and SS-l inhibited the release of B-hexosaminidase from RBL-2H3 cells, although their water extracts were inactive.
Abstract The high affinity binding site for human immunodeficiency virus (HIV) envelope glycoprot... more Abstract The high affinity binding site for human immunodeficiency virus (HIV) envelope glycoprotein gp120 resides within the amino-terminal domain (D1) of CD4. Mutational and antibody epitope analyses have implicated the region encompassing residues 40-60 in D1 as the primary binding site for gp120. Outside of this region, a single residue substitution at position 87 abrogates syncytium formation without affecting gp120 binding.
Together with protein tyrosine kinases (PTKs), protein tyrosine phosphatases (PTPs) serve as hall... more Together with protein tyrosine kinases (PTKs), protein tyrosine phosphatases (PTPs) serve as hallmarks in cellular signal transduction by controlling the reversible phosphorylation of their substrates. The human genome is estimated to encode more than 100 PTPs, which can be divided into eleven sub-groups according to their structural and functional characteristics.
Matrix metalloproteinases (MMPs), a family of zinc-containing endopeptidases, participate in many... more Matrix metalloproteinases (MMPs), a family of zinc-containing endopeptidases, participate in many normal processes such as embryonic development and wound repair, and in many pathological situations such as cancer, atherosclerosis, and arthritis. Peptidomimetic MMP inhibitors were designed and synthesized with N-formylhydroxylamine (retrohydroxamate) as a zinc-binding group and various side chains on the α, P1', and P2'positions. Using in vitro MMP assays with purified MMPs (MMP-1, MMP-2, MMP-3, MMP-9, and MMP-14) and ...
Protein kinases are a diverse group of enzymes that are involved in fundamental cellular mechanis... more Protein kinases are a diverse group of enzymes that are involved in fundamental cellular mechanisms such as sensing and signaling in a variety of environmental or intracellular cues, controlling of gene expression and playing crucial role in various metabolisms. To initiate functional studies, we are constructing a bacterial kinase library. Five target kinases related to the development of antimicrobial agents or diagnostic targets and the metabolic engineering were investigated through the various public databases. Three hundred and ...
Anthraquinones have been reported as phosphatase inhibitors. Therefore, anthraquinone derivatives... more Anthraquinones have been reported as phosphatase inhibitors. Therefore, anthraquinone derivatives were screened to identify a potent phosphatase inhibitor against the phosphatase of regenerating liver-3 (PRL-3). Emodin strongly inhibited phosphatase activity of PRL-3 with IC(50) values of 3.5μM and blocked PRL-3-induced tumor cell migration and invasion in a dose-dependent manner. Emodin rescued the phosphorylation of ezrin, which is a known PRL-3 substrate. The results of this study reveal that emodin is a PRL-3 inhibitor and a good lead molecule for obtaining a selective PRL-3 inhibitor.
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Papers by Seong Eon Ryu