Papers by Nazeerullah Rahamathullah
System-Enabled Microbial Cell Factories for the Production of Biomolecules
Apple Academic Press eBooks, Mar 13, 2024
medRxiv (Cold Spring Harbor Laboratory), Jan 29, 2024
The aim of this study was to assess the prevalence of class A ESBL, class B and D carbapenemase e... more The aim of this study was to assess the prevalence of class A ESBL, class B and D carbapenemase encoding genes (bla CTX-M , bla TEM , bla SHV , bla NDM, bla IMP and bla ) in gram-negative bacterial pathogens isolated from various clinical samples collected from northern region of UAE. A laboratory based experimental study was conducted from October 14, 2021, to June 30, 2022. A total of 3670 various clinical samples (urine, blood, pus, and sputum) were obtained from patients attending the in and out-patient departments of Thumbay University Hospital and various other hospitals of Northern Emirates of UAE, processed for routine bacterial culture examination and determined their antibiotic sensitivity pattern especially ESBL and carbapenem resistance using MicroScan Neg Breakpoint Combo 50 panel. Molecular detection of class A ESBL, class B and D carbapenemase encoding genes (blac TX-M , bla TEM , bla SHV bla NDM , bla IMP & bla OXA-48 ) were performed on the ESBL and carbapenemase producing gram negative bacteria. Partial genomic sequencing and phylogenetic tree analysis was performed on the most predominant ESBL gene bla CTX-M . In total, 1098 bacterial cultures were obtained, of which 833 were gram negative bacteria and among them, 209 (25.1%) were ESBL producers and 40 (4.8%) were both ESBL and carbapenemase producers. In all the 249 bacterial isolates, the ESBL and carbapenemase encoding genes (bla CTX-M, bla TEM , bla SHV , bla NDM & bla IMP ) were detected except bla OXA-48 . The bla CTX-M (n=72) was the predominantly harbored gene followed by bla TEM (n=56) and bla SHV (n=29), mostly detected in E. coli (n=157), then K. pneumoniae (n=41), P. aeruginosa (n=18) & A. baumannii (n=7). The bla NDM & bla IMP were detected in K. pneumoniae (n=4), A. baumannii (n=3) and P. aeruginosa (n=2) for bla IMP . The gene combinations such as bla CTX-M+TEM (n=15), bla CTX- M+SHV (n=15), bla CTX-M+TEM +bla SHV (n=6) were co-harbored in 36 E. coli and in the K. pneumoniae, the bla CTX-M+TEM (n=15), bla CTX-M+SHV (n=15), bla TEM+SHV (n=3), bla TEM+NDM (n=1) were detected. The bla CTX-M+TEM, bla CTX -M+SHV & bla CTX-M+TEM+SHV+IMP were detected in one P. aeruginosa and in one A. baumannii, the bla TEM+NDM , bla TEM+IMP & bla CTX-M+TEM+SHV+IMP were detected. The highest bacterial culture positivity was detected in the urine samples. 25.1% of the Gram-ve bacteria were only ESBL producers and 4.8% were both the ESBL & carbapenemase producers. 44.5% of ESBL & carbapenemase producing bacteria harbored the bla CTX-M and mostly detected in the E. coli, then K. pneumoniae and P. mirabilis. Multiple gene combinations were detected in 5% P. aeruginosa, 10% A. baumannii and 3.82% E. coli. This finding highlights the importance of molecular detection of ESBL & carbapenemase producing genes to emphasize the monitor and control the development of multi drug resistant Gram-ve bacterial pathogens.
Curēus, May 16, 2024
Objective: To describe the histopathological pattern of different breast lesions among tissue spe... more Objective: To describe the histopathological pattern of different breast lesions among tissue specimens sent to our laboratory. Method: A record-based study using a retrospective review of 255 histologically diagnosed breast biopsy reports in the histopathology department of authors from December 2016 to November 2021 was conducted. The specimens were collected from core biopsy, lumpectomy, and mastectomy. All data obtained were analyzed using the Statistical Package for the Social Sciences (SPSS) version 28 (IBM SPSS Statistics, Armonk, NY). Then, the findings were presented using text, tables, and charts. Result: A total of 255 breast lesions were analyzed in this study. Most of the cases were benign (58.8%), followed by inflammatory lesions (21.6%), and malignant (19.6%). Fibroadenoma was the most prevalent benign lesion (36.7%), and most of the patients (70.9%) were in the age group of 20-39 years old. The most common inflammatory lesion was granulomatous mastitis (56.4%), and most of the cases were diagnosed in the age group of 30-39 years old. Invasive ductal carcinoma (IDC) was the most encountered histological type of carcinoma (62%). Of the malignant cases, 52% were diagnosed before the age of 50 years. Among these 50 cases, grade 2 was the most prevalent one (46%). Conclusion: Benign breast lesions are more common than malignant breast lesions, and fibroadenoma is the most common benign subtype. Granulomatous mastitis is the most prevalent inflammatory breast lesion. About two-thirds of malignant cases are non-Arab. Invasive ductal carcinoma with no special type (NST) is the most common malignant subtype.
PLOS ONE, 2023
Objective The aim of this study was to explore the prevalence of low and high-risk HPV genotypes ... more Objective The aim of this study was to explore the prevalence of low and high-risk HPV genotypes in PAP smear samples of women in northern region of the UAE using HPV direct flow CHIP method. Methods A cross-sectional retrospective study was conducted between September 2021 to April 2022. A total of 104 liquid-based cervical cytology samples were obtained from women aged 20–59 years attending the Gynaecology out-patient department of Thumbay University Hospital and other hospitals of Northern Emirates of UAE, processed for the routine cytological examination to identify and differentiate morphological changes of the PAP smear samples. HPV genotyping was performed using HPV direct flow CHIP method. Results In total, 112 HPV genotypes were detected in 63 women (60.57%) included 18 abnormal cytological and 45 normal epithelial samples. 63 LR and 49 HR HPV genotypes were identified in all the 63 positive samples. Highest rate of infection with multiple LR and HR HPV genotypes were detec...
International journal of health sciences, 2022
The current study aimed to determine the prevalence of class A genes in the Extended Spectrum of ... more The current study aimed to determine the prevalence of class A genes in the Extended Spectrum of β-Lactamase producing E. coli and K. pneumoniae isolated from Patients Urine Samples in United Arab Emirates. A total of 3457 urine samples were collected from October 2018 to January 2019. ESBL screening and confirmatory test were done using MicroScan WalkAway automated bacterial identification system. The ESBL class A gene primers were used to detect the blaCTX-M, blaSHV & blaTEM. Out of the collected urine samples, 474 were reported positive for urinary tract infection. A total of 130 samples were identified as Gram negative ESBL producing species, Escherichia coli (n=107) and Klebsiella pneumoniae (n=23). Amid these 130 ESBL producers, the class A ESBL genes blaTEM, blaCTX-M & blaSHV were detected in 78 isolates of which 59 were E. coli and 19 K. pneumoniae. In the identified 59 E. coli isolates, the most predominant gene was blaTEM, followed by blaCTX-M and blaSHV, and these three g...
Effect of different antidiabetic medications on atherosclerotic cardiovascular disease (ASCVD) risk score among patients with type-2 diabetes mellitus: A multicenter non-interventional observational study
PLOS ONE
Objective The aim of this study was to compare the clinical outcomes associated with different co... more Objective The aim of this study was to compare the clinical outcomes associated with different combinations of oral diabetic drugs among patients with type 2 diabetes mellitus. Method A prospective multicenter longitudinal, noninterventional observation study design was applied. At baseline (0 month), clinical parameters including glucose profile, renal function, lipid profile and risk assessment for cardiovascular risks were calculated. Mean Weighted difference (MWD) with heterogeneity and effect z was calculated to determine the risk reduction at the end of the study. Results A total of 1,657 were enrolled to different cohorts with response rate of 75.5%. The distribution of patients was based on prescribed drug. A total of 513 (30.9%) in G1 (metformin alone), 217 (13.09%) in G2 (metformin with Glimepiride), 231 (12.85%) in G3 (Metformin with Gliclazide), 384 (23.17%) in G4 (metformin with Sitagliptin) and 312 (18.89%) in G5 (Metformin with Saxagliptin). There was no significant d...
Prevalence of extended spectrum beta-lactamase producing Enterobacteriaceae in urine samples from Thumbay hospitals, U.A.E
Access Microbiology
Biodiversity and biochemical characterization of bacteria from terrestrial subsurface environment
Access Microbiology
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Papers by Nazeerullah Rahamathullah