Papers by Seyed Akbar Khodaparast
Powdery mildew is a common disease of Spiraea spp. worldwide; however, there are no reports of th... more Powdery mildew is a common disease of Spiraea spp. worldwide; however, there are no reports of this destructive disease in Spiraea species from Iran. In recent years, severe powdery mildew symptoms have been observed in Spiraea spp. throughout the Guilan Province. In this study, we collected infected plant specimens and used morphological and molecular approaches to identify the powdery mildew fungus involved in this disease. We did not observe the teleomorphic state of the fungus in this area, and it appears that the fungus occurred only in the anamorphic state. At least six species of Podosphaera occur worldwide, and there is a considerable overlap between the anamorphic characteristics of some species. Hence, the ITS sequence was used to identify common powdery mildew species occurring in Spiraea in the Guilan Province. The results showed that the ITS sequence is useful for Podosphaera species in Spiraea. Podosphaera minor Howe. was identified as the causal agent of powdery mildew disease in this plant. To our knowledge, this is the first report of Podosphaera minor as a fungus in Iran.
In this study, the phylogeny and taxonomy of Erysiphe sect. Microsphaera was investigated for the... more In this study, the phylogeny and taxonomy of Erysiphe sect. Microsphaera was investigated for the first time in Iran. This study was based on morphological examination and rDNA (ITS+ LSU) sequencing. We examined 80 voucher specimens from the Fungus Reference Collection of Herbarium Ministerii Iranici Agriculturae (IRAN) and the University of Guilan Mycological fungarium (including specimens newly collected during 2019-2021). Seventy-nine sequences covering 20 species were generated. E. hyperici was not available for this study. Sequencing of E. begoniicola and E. tortilis failed. Based on our findings, E. coluteae and E. crispula were sequenced for the first time in this study. Records of E. erlangshanensis and E. castaneigena from Iran were rejected. The number of accepted species in sect. Microsphaera increased from 13 in 2009 to 23 in this study. Erysiphe crispula on Astragalus spp. is reported here for the first time, and Syringa vulgaris has been reported as a new host of E. syringae-japonicae in Iran. Furthermore, DNA barcodes, colored plates, digital illustrations, and identification keys of all E. sect. Microsphaera in Iran are provided herein.
Within the framework of the Mycology research laboratory's program at the University of Guilan fo... more Within the framework of the Mycology research laboratory's program at the University of Guilan focusing on the identification and DNA barcoding of powdery mildew fungi (Erysiphaceae, Ascomycota) from Iran, a powdery mildew specimen on Helichrysum was collected from Zanjan-Tarom road and subjected for morphological and molecular identification. Although this species morphologically resembles Leveillula helichrysi, and some other closely related species such as L. taurica complex, the ITS sequence differentiates this fungus from the sequences of L. helichrysi and L. taurica available in GenBank. We suggest this collection may represent a new species or new haplotype within L. helichrysi. Therefore, until we obtain more samples and sequence the type material, we report this fungus as Leveillula cf. helichrysi from Iran.
Species of Phyllosticta are important group of pathogenic and endophytic fungi that are reported ... more Species of Phyllosticta are important group of pathogenic and endophytic fungi that are reported from various crop plants, ornamental and non-fruiting plants. These fungi cause several diseases such as leaf and fruit spots and fruit rot. In order to identify Phyllosticta species in Guilan province, several collections were examined around the region. Altogether 14 taxa were isolated from different plant species. Plant species viz. Prunus laurocerasus, Danae racemosa, Aesculus carnea, Camellia japonica, Citrus sinensis, Rubus sp., Schefflera sp., Diospyros kaki and Ginkgo biloba are recorded as new host for Phyllosticta spp. in Iran. Magnolia soulangeana, Camellia sinensis, Citrus limetta and Rosa sp. are also reported as host plants of Phyllosticta species in this province. Precise recognition of Phyllosticta sp. needs both morphological and molecular studies. Hence, in this study P. theacearum was distincly identified; one collection on Citrus sp. was closely related to P. citricar...
In this study, we report some Ascomycete genera from Nectriaceae, Botryosphaeriaceae, Cryphonectr... more In this study, we report some Ascomycete genera from Nectriaceae, Botryosphaeriaceae, Cryphonectriaceae and Hypocreaceae families from Guilan province, Iran. Cultural and morphological characteristics and sequence information of rDNA ITS and translation elongation factor 1-α encoding gene (tef1) were used for identification. Among isolates studied in this research, six species viz. Microthia havanensis, Lasiodiplodia hormozganensis, Lasiodiplodia parva, Thyronectria austroamericana, Trichoderma cf. chromospermum and Trichoderma cf. longibrachiatum are reported as new or less known species for the region. As a result, two genera viz. Thyronectria (Nectriaceae) and Microthia (Cryphonectriaceae) and two species (Lasiodiplodia parva and Trichoderma cf. chromospermum)are new reports for Iran mycobiota.
Rostaniha, 2008
During a study several species of mitosporic fungi which have been collected from Guilan Province... more During a study several species of mitosporic fungi which have been collected from Guilan Province were examined and seven species viz.: Hansfordia pulvinata, Myriellina cydoniae, Phloeospora cf. ulmi, Questieriella sp., Sarcinella questieri, Septoria cf. castaneicola and Sirosporium ...
During the study on boxwood blight from 2016 to 2017, some isolates of Pseudonectria were isolate... more During the study on boxwood blight from 2016 to 2017, some isolates of Pseudonectria were isolated from boxwood leaves and branches. Fungi were isolated and purified using conventional methods. According to the morphological characteristics and ITS rDNA sequences, two species of Pseudonectria viz. P. buxi and P. foliicola were identified. Moreover, the pathogenicity of these two species under laboratory and greenhouse conditions was examined. None of the species were able to produce disease on the healthy plant leaves, and leaf wounds were necessary to establish the infection. According to our knowledge, this is the first report of Pseudonectria species on boxwood from Iran.
Nine Dictyuchus isolates were obtained from floating and decaying twigs and leaves in Anzali lago... more Nine Dictyuchus isolates were obtained from floating and decaying twigs and leaves in Anzali lagoon, Anzali County, Guilan province, Iran. They are interestingly distinguished from current Dictyuchus species by the presence of dictyoid and achlyoid type of zoospore discharge, abundant fusiform and spherical gemma, and the absence of any sexual apparatus. In addition, phylogenetic analyses of the internal transcribed spacer (ITS) region of the nuclear rDNA and cytochrome c oxidase subunit I (cox1) gene sequences using Maximum likelihood method indicate its novelty. We preferred not to introduce the isolates as new species due to low number of and sometimes unreliable sequences and lack of type species and suggested to verify Dictyuchus sterilis which has been excluded before. We discussed about taxonomy of the genus in details and provided a revised key to the species. In addition, it was shown that sterility in vitro might be common in this genus.
American Journal of Applied Sciences, 2011
Sooty mold fungi are often associated with honeydew which insects secrete while feeding on the pl... more Sooty mold fungi are often associated with honeydew which insects secrete while feeding on the plant. A great variety of these fungi occur in the North of Iran, especially on Citrus ssp which is one of the most widely cultivated fruit trees in this region. During last 10 years, several collections of these fungi examined and 15 species have been recorded. In this paper seven previously unreported species are treated and described for the first time from Iran. The treated species include Catenuloxyphium heterosporum (on Salix aegiptiaca and Alnus glutinosa); Echinothecium sp. (on Eriobotrya japonica and Passiflora sp.); cf. Denisiella sp. (On Citrus sinensis and Malus pumila); Fumagospora capnodioides (on Alnus glutinosa, Populus deltoids, Salix aegyptiaca and Zelkova carpinifolia); Leptoxyphium fumago (on Alnus glutinosa, Rubus sp. and Phytolacca americana); Tripospermum juglandis (on Ligustrum sp.) and Scorias spongiosa (on Citrus sinensis).
Microorganisms
Contributions of fungal and oomycete communities to freshwater carbon cycling have received incre... more Contributions of fungal and oomycete communities to freshwater carbon cycling have received increasing attention in the past years. It has been shown that fungi and oomycetes constitute key players in the organic matter cycling of freshwater ecosystems. Therefore, studying their interactions with dissolved organic matter is crucial for understanding the aquatic carbon cycle. Therefore, we studied the consumption rates of various carbon sources using 17 fungal and 8 oomycete strains recovered from various freshwater ecosystems using EcoPlate™ and FF MicroPlate™ approaches. Furthermore, phylogenetic relationships between strains were determined via single and multigene phylogenetic analyses of the internal transcribed spacer regions. Our results indicated that the studied fungal and oomycete strains could be distinguished based on their carbon utilization patterns, as indicated by their phylogenetic distance. Thereby, some carbon sources had a higher discriminative strength to categor...
Arthrocladiella is a monotypic member of family Erysiphaceae (Ascomycota, Helotiales), which has ... more Arthrocladiella is a monotypic member of family Erysiphaceae (Ascomycota, Helotiales), which has only been reported from Lycium spp. (Solanaceae). Morphological data supplemented with rDNA ITS sequence confirmed that the Arthrocladiella mougeotii infects Lycium sp. in Iran. Moreover, Arthrocladiella is reported as a new genus for Iranian Mycobiota.
Athelia rolfsii is a globally dispersed pathogenic fungus, causing white root rot disease in many... more Athelia rolfsii is a globally dispersed pathogenic fungus, causing white root rot disease in many crops and horticultural plants. In this study, 90 isolates were collected from three provinces of Guilan, Mazandaran and Golestan in northern Iran. Eighteen isolates were selected for sequence analyses based on their host, sampling sites and Mycelial Compatibility Groups (MCG). Translation elongation factor 1-α(tef1-α), β-tubulin (tub2) genes and rDNA large subunit (LSU) were partially amplified and sequenced in order to conduct phylogenetic analyses. Approximately, all Iranian isolates clustered together in both tef1-α and LSU phylogenetic trees. However, after deletion of ambiguous sites, no variations were observed in tub2 sequences. ISSR and SCoT were also used to investigate the genetic structure of the population. Results of the molecular variance analysis (AMOVA) showed that 70 and 30% of the observed variance corresponded to the difference between and within the populations, res...
Rustanī/hā, 2019
هیفومیستهای آبزی تولیدکننده تعدادی زیادی آنزیمهای خارج سلولی هستند که در تجزیه زیستی آلودگیها... more هیفومیستهای آبزی تولیدکننده تعدادی زیادی آنزیمهای خارج سلولی هستند که در تجزیه زیستی آلودگیهای ناشی از فعالیتهای انسانی، پلاستیک، ترکیبات آروماتیک و هیدروکربنهای نفتی نقش دارند. مطالعه حاضر با هدف افزایش دانش ما از هیفومیستهای آبزی در ایران صورت گرفت. نمونهبرداری طی سال 1396 از تالاب انزلی در استان گیلان انجام گرفت. از میان 55 جدایه قارچی که در این مطالعه به دست آمد، تعداد هشت گونه شامل <em>Arthrobotrys oligosporus</em>، <em>Ceratorhiza hydrophila</em>، <em>Fusarium </em>cf. <em>ensiforme</em>، <em>F. incarnatum </em>species complex، <em>Myrmecridium schulzeri</em>،<em>Paecilomyces variotii</em>، <em>Sarocladium subulatum</em> و <em>Volutella citrinella</em> برمبنای خصوصیات ریختشناختی شناسایی شدند. علاوه براین، سه گونه متعلق به جنسهای <em>Arthrobotrys </em>، <em>Fusarium </em> و<em>Sarocladium </em> ناشناخته باقی ماندند. شناسایی مو...
Hellenic Plant Protection Journal, 2022
Summary Stem rot caused by Athelia rolfsii (syn. Sclerotium rolfsii), is an important disease of ... more Summary Stem rot caused by Athelia rolfsii (syn. Sclerotium rolfsii), is an important disease of peanut in Iran. Soil samples were collected from 15 peanut fields before the 2014 growing season in Guilan province, Iran and the viable sclerotia were counted using the soil-tray technique. The 15 selected fields were also evaluated for disease incidence at three intervals during the growing seasons of 2013 and 2014. The disease incidence at the end of the growing season ranged from 0 to 29.3% and from 0 to 45% in 2013 and 2014, respectively, depending on the field. The disease progress model, which was introduced for each field based on the disease incidence, showed good fitness with the monomolecular model in both years, but especially in 2014. Disease severity was evaluated in three out of the 15 peanut fields at three intervals during the growing season of 2014. The monomolecular model could describe more than 88% of the data. There was a significant linear relationship between the ...
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Papers by Seyed Akbar Khodaparast