Se analizó la macrofauna del suelo de cuatro ambientes naturales y agroecosistemas localizados en... more Se analizó la macrofauna del suelo de cuatro ambientes naturales y agroecosistemas localizados en zonas altas de la eco-región Esteros del Iberá (Corrientes, Argentina) con distintos grados de perturbación, a fin de contribuir a evaluar el impacto de las actividades productivas en este humedal. Se determinó la diversidad, abundancia, densidad y biomasa de los principales grupos y se compararon las comunidades analizadas. Se aplicó el protocolo de Conservación y Manejo Sustentable de la Biodiversidad del Suelo modificado, en cuatro puntos en cada sitio. Se analizó la influencia de distintos factores de perturbación sobre la biota. La macrofauna del suelo estuvo integrada por 19 órdenes de invertebrados, principalmente oligoquetos terrestres y artrópodos, entre los cuales las termitas, hormigas y coleópteros fueron los más abundantes. Las diferencias entre los ensambles analizados se evidenciaron en su composición taxonómica, diversidad, número de órdenes y densidad de los grupos más abundantes, pero ellas no fueron estadísticamente significativas para esta última característica. La mayoría de los individuos se detectaron en el estrato superficial del suelo (0-10 cm) y la biomasa fue más elevada en los pastizales aunque los órdenes de mayor biomasa relativa fueron distintos en cada sitio. Las similitudes entre ambientes agruparon por un lado al cultivo de algodón y al pastizal forestado, y por otro al bosque y al pastizal sometido a pastoreo. Dos de las perturbaciones ambientales analizadas (reemplazo de la vegetación natural y remoción del suelo) influyeron sobre las comunidades macroedáficas. Las hormigas y lombrices resultaron posibles invertebrados bioindicadores en estas comunidades, en tanto que las termitas subterráneas resultaron importantes por su diversidad y biomasa, principalmente en los dos ambientes con mayor grado de perturbación. Palabras clave. Invertebrados del suelo, agroecosistemas, perturbaciones antrópicas.
Background Envenomation by the South American Lonomia saturniid caterpillars, named lonomism, con... more Background Envenomation by the South American Lonomia saturniid caterpillars, named lonomism, constitutes an emerging and somewhat neglected public health issue in Argentina and neighboring countries. Considering that there is an intricate relationship between environment and human health in such cases, this study aimed to analyze the eco-epidemiological profile of 40 accidents and 33 occurrences of Lonomia spp. in Misiones (Argentina) between January 2014 and May 2020. Methodology/Principal findings We described the eco-epidemiological variables and characterized the abiotic scenario of such cases. Additionally, we obtained a density map that shows the punctual intensity of Lonomia records throughout Misiones. Most of the accidents occurred in the Department of Guaraní and involved male victims younger than 20 years old. The accidental/occasional occurrence of Lonomia spp. (considering both adult and caterpillar stages together) was significantly higher in the rural area, whereas o...
Se analizo la macrofauna del suelo de cuatro ambientes naturales y agroecosistemas localizados en... more Se analizo la macrofauna del suelo de cuatro ambientes naturales y agroecosistemas localizados en zonas altas de la eco-region Esteros del Ibera (Corrientes, Argentina) con distintos grados de perturbacion, a fin de contribuir a evaluar el impacto de las actividades productivas en este humedal. Se determino la diversidad, abundancia, densidad y biomasa de los principales grupos y se compararon las comunidades analizadas. Se aplico el protocolo de Conservacion y Manejo Sustentable de la Biodiversidad del Suelo modificado, en cuatro puntos en cada sitio. Se analizo la influencia de distintos factores de perturbacion sobre la biota. La macrofauna del suelo estuvo integrada por 19 ordenes de invertebrados, principalmente oligoquetos terrestres y artropodos, entre los cuales las termitas, hormigas y coleopteros fueron los mas abundantes. Las diferencias entre los ensambles analizados se evidenciaron en su composicion taxonomica, diversidad, numero de ordenes y densidad de los grupos mas ...
teorie informace a automatizace AVČR, v.v.i. Abstrakt: Tato práce se zabývá návrhem systematickéh... more teorie informace a automatizace AVČR, v.v.i. Abstrakt: Tato práce se zabývá návrhem systematického kombinování diskrétních pravděpodobnostních distribucí založeném na teorii rozhodování a teorii informace, konkrétně na křížové entropii (známé také jako Kullbackova-Leiblerova (KL) divergence). Optimální kombinací je pravděpodobnostní funkce minimimalizující podmíněnou střední hodnotu KL-divergence. Hustota pravděpodobnosti, která se váže k této střední hodnotě, rovněž minimalizuje KL-divergenci za podmínek vztažených křešenému problému. Ačkoliv je kombinace odvozena pro pravděpodobnostní typ informace na společném nosiči, můžeme ji po transformaci a/anebo rozšíření použít i pro míchání jiných typů informace. Práce také zahrnuje diskuzi o navrhovaném kombinování a sekvenčním zpracování dat, opakujících se datech, ovlivnění výsledků preferencemi mezi zdroji informace a aplikaci na reálná data. Klíčová slova: teorie distribuovaného rozhodování, Kullbackova-Leiblerova divergence, princip minimální křížové entropie Title: Cross-entropy based combination of discrete probability distributions for distributed decision making
Atmospheric plasma-based approaches for the degradation of dimethyl phthalate (DMP) in water
Journal of Environmental Management, 2022
Cold plasma based treatment of contaminated water is becoming a promising novel green remediation... more Cold plasma based treatment of contaminated water is becoming a promising novel green remediation option. This study assessed the performance of two different cold plasma reactors, using, respectively, a self-pulsing discharge (SPD) and a multipin corona discharge (MCD), in the degradation of dimethyl phthalate (DMP), a persistent and ubiquitous pollutant of the aquatic environment. The process kinetics and energy efficiency, as well as the main plasma generated reactive species were determined under various operating conditions concerning the plasma feed gas and flowrate, the voltage polarity, the input power, the DMP initial concentration, the liquid conductivity, and the aqueous matrix used to prepare DMP solutions for these experiments. The MCD reactor, operated with air as plasma feed gas and negative voltage polarity, gave the best results in terms of rate and energy efficiency. Moreover, variations in plasma input power and in the liquid conductivity have limited effect on DMP degradation rate, making this reactor suitable for treating liquids with a range of initial conductivities The effects of DMP initial concentration on its rate of degradation and on the process energy efficiency were also investigated. Differences in the efficiency of production and distribution of plasma generated reactive species, notably •OH and H2O2, observed for the two tested reactors are discussed in terms of different extension of the plasma/liquid interface and diffusion into the bulk solution. It is proposed that among the reactive species, •OH foremost, and O3 to a lesser extent, play a pivotal role in DMP degradation, while the contribution of H2O2 appears to be limited. The rate of DMP degradation was not drastically different in Milli-Q water and in tap water, a positive outcome in view of practical applications of the technology. The lower rate observed in tap than in Milli-Q water is attributed to the presence of bicarbonate and carbonate, which are known scavengers of hydroxyl radicals.
Objectives Positron-emission tomography (PET) has improved identification of the primary tumor as... more Objectives Positron-emission tomography (PET) has improved identification of the primary tumor as well as occult nodal burden in cancer of the head and neck. Nevertheless, there are still patients where the primary tumor cannot be located. In these situations, the standard of care is comprehensive head and neck radiation therapy however it is unclear whether this is necessary. This study examines the effects of radiation treatment volume on outcomes among using data from two cancer centers in unknown primary carcinoma of the head and neck. Methods Patients received unilateral (n = 34), or bilateral radiation (n = 28). Patient factors such as age, gender, smoking history, and patterns of failure were compared using Mann Whitney U and Chi Square. Overall survival (OS) and disease free survival (DFS) trends were estimated using Kaplan-Meier survival curves. Effect of treatment volume on survival was examined using multivariate cox proportional hazard regression model. Results No significant differences were observed in the frequency of local (p = 0.32), regional (p = 0.50), or distant (p = 0.76) failures between unilateral and bilateral radiation therapy. By Kaplan-Meier estimates, OS (3-year OS bilateral = 71.67%, unilateral = 77.90%, p = 0.50) and DFS (3-year DFS bilateral = 77.92%, unilateral = 69.43%, p = 0.63) were similar between the two treatment approaches. Lastly, multivariate analysis did not demonstrate
pero estos presentan, generalmente, un perfil nutricional poco adecuado dentro de una alimentació... more pero estos presentan, generalmente, un perfil nutricional poco adecuado dentro de una alimentación saludable. La tecnología de Fritura al vacío se presenta como una clara opción de procesamiento para el desarrollo de nuevos productos y de los ya existentes. Este artículo de revisión presenta las ventajas del uso de la Tecnología de Fritura al Vacío en diferentes tipos de alimentos, especialmente en relación al contenido de algunos micronutrientes y grasa. Se mencionan además los trabajos efectuados en Ecuador, especialmente en productos autóctonos.
BRAZILIAN JOURNAL OF AGRICULTURE - Revista de Agricultura, 2017
The study of the developmental pathways in termites contributes to the understanding of caste evo... more The study of the developmental pathways in termites contributes to the understanding of caste evolution and resources utilization, as well as other ecological aspects. In this paper, the caste developmental pathways of Cortaritermes fulviceps (Silvestri) were analyzed in natural colonies from the Province of Corrientes, Argentina. Five measurements were taken of different body parts of larvae (L), workers (W), pre-soldiers (PS), soldiers (S), nymphs (N) and alates (A) for morphometric analyses. Dissections were also performed to determine the sex of workers and soldiers. The collected data were analyzed using principal component analysis, analysis of variance and Tukey test. In the nymphal line, four nymphal instars and alates were identified. The apterous line included two larval instars. Major workers were females and minor workers, presoldiers and soldiers were males. The results suggest that the developmental pathways of C. fulviceps is similar to the one of the genus Nasutitermes Dudley.
Se estudió la influencia de técnicas de clarificación en vino de mora de Castilla Rubus glaucus B... more Se estudió la influencia de técnicas de clarificación en vino de mora de Castilla Rubus glaucus Benth sobre su cromaticidad, turbidez y rendimientos del proceso. Inicialmente con la microfiltración tangencial (MFT) a tres diferentes presiones de trabajo (1.5, 2.0 y 2.5 bar) y complementariamente con el pretratamiento de la alimentación con bentonita sódica. La MFT a 1.5 bar obtuvo el mayor Factor de Retención Volumétrica (FRV) y el mejor rendimiento, estandarizando las condiciones de presión para la MFT con pretratamiento. En función del pH, en 9 gramos se estableció la cantidad óptima de floculante añadido a cada lote de vino, llevado a reposo a 6°C (6, 12 y 24 horas), obteniéndose 610 ± 38 g para la mayor proporción de turbios en el lote de 6 horas de reposo, producto de una mayor captación inestable por parte del clarificante. La MFT de los pretratamientos indica mejoras representativas de hasta 40% en el rendimiento. Todas las muestras analizadas de vino microfiltrado con y sin ...
Revista Ecuatoriana de Medicina EUGENIO ESPEJO, 2019
El calostro bovino es una secreción mamaria que se genera aproximadamente siete días antes del pa... more El calostro bovino es una secreción mamaria que se genera aproximadamente siete días antes del parto para alimentar al becerro. El contenido de sus componentes puede llegar a ser 100 veces mayor que en la leche cruda y estudios de otros autores señalan los beneficios alimenticios y medicinales que este presenta. En tal virtud, se estudió el efecto de la aplicación de microfiltración tangencial sobre la concentración de proteínas de calostro bovino como una fuente alimenticia y medicinal. El estudio consideró vacas de la raza Holstein y sus entrecruzamientos, desarrolladas en el cantón Cayambe. Los análisis químicos (grasa, proteína total, inmunoglobulinas totales, lactosa, sólidos totales y sólidos no grasos) fueron analizados en un equipo MilkoScan, los microbiológicos (contaje de células somáticas y contaje total) en un analizador Bacto Scan, la cuantificación de inmunoglobulina G con un cromatógrafo líquido Varian y la influencia de la aplicación de microfiltración tangencial con...
This paper reports the characterization of a low-power, atmospheric pressure plasma source develo... more This paper reports the characterization of a low-power, atmospheric pressure plasma source developed for and successfully tested in applications of plasma medicine. The source produces plasma by ionizing a helium flow containing traces of air. The effluent gas is thus enriched in reactive oxygen and nitrogen species as well as helium metastable excited states. Electrical measurements, Schlieren imaging, OES and various chemical analyses and probes enabled us to fully characterize the plasma source and to determine some fundamental parameters for treating living samples such as the power feeding the discharge, the optimal source to target distance, the maximum exposure time, and to identify the major chemical species responsible for the biological action on exposed living matter. K E Y W O R D S atmospheric plasma jet, optical emission spectroscopy, plasma medicine, reactive oxygen species, Schlieren 1 | INTRODUCTION Plasma medicine is the emerging discipline dealing with the use of cold, atmospheric pressure plasmas as a tool to interact with living matter for therapeutic purposes. [1-4] The basic requirement for plasma sources to be applicable in this field is to produce a diffuse plasma at atmospheric pressure with power levels low enough to keep the treated substrate temperature below 37°C, which means typically a few Watts of dissipated power. The most used process gases are helium and argon, mixed with small fractions of oxygen, nitrogen, Gabriele Neretti and Francesco Tampieri share the first authorship.
The potassium channel Kv1.3 is highly expressed in the mitochondria of various cancerous cells. H... more The potassium channel Kv1.3 is highly expressed in the mitochondria of various cancerous cells. Here we show that direct inhibition of Kv1.3 using two mitochondria-targeted inhibitors alters mitochondrial function and leads to reactive oxygen species (ROS)-mediated death of even chemoresistant cells independently of p53 status. These inhibitors killed 98% of ex vivo primary chronic B-lymphocytic leukemia tumor cells while sparing healthy B cells. In orthotopic mouse models of melanoma and pancreatic ductal adenocarcinoma, the compounds reduced tumor size by more than 90% and 60%, respectively, while sparing immune and cardiac functions. Our work provides direct evidence that specific pharmacological targeting of a mitochondrial potassium channel can lead to ROS-mediated selective apoptosis of cancer cells in vivo, without causing significant side effects.
The European Physical Journal Applied Physics, 2016
We are pleased to introduce this special issue which collects a selection of contributions to the... more We are pleased to introduce this special issue which collects a selection of contributions to the sixth Central European Symposium on Plasma Chemistry, CESPC-6, held in Bressanone, Italy, during September 6-10, 2015. The conference, which received support by CEI-the Central European Initiative, gathered and provided a lively forum for scientists, including many PhD students and young post-docs, from 20 different countries and 4 continents. The scientific focus of the conference was on major areas of fundamental studies and applications of plasma, and included: fundamentals, modelling and diagnostics, new materials, energy technologies, environmental protection, bio-and medical-plasma technologies, surface processes. This selection of papers is well representative for the conference scientific contents. We would like to thank all the Authors and Referees for their efforts in preparing and reviewing the manuscripts, as well as the editorial office of the EPJAP for its helpful assistance in organizing this special issue.
Biochimica et Biophysica Acta (BBA) - Bioenergetics, 2016
Mitochondria are involved in cell signalling, proliferation, differentiation and apoptosis. Their... more Mitochondria are involved in cell signalling, proliferation, differentiation and apoptosis. Their participation in these processes depends on the production of ATP and ROS, both generated by electron transfer reactions. Bioenergetics has become central to our understanding of pathological mechanisms as well as for the development of new therapeutic strategies and as a tool for gauging disease progression in cancer, neurodegeneration, diabetes and cardiovascular disease. The view is emerging that mitochondrial inner membrane potassium channels have a profound effect on organelle function and, consequently, on the survival of the whole cell. Recently, we provided evidence that direct inhibition of the mitochondrial Kv1.3 channel by two novel mitochondria-targeted derivatives of PAP-1, a specific inhibitor of Kv1.3, induces apoptosis. Direct actions of these drugs on mitochondria are indicated by immediate swelling, loss of mitochondrial membrane potential, release of reactive oxygen species (ROS) and altered respiration. Both drugs efficiently kill melanoma and pancreatic ductal adenocarcinoma (PDAC) cancer cells in vitro in the microMolar range. Furthermore, they induced apoptosis in ex-vivo primary tumor cells from chronic B-lymphocytic leukemia, while sparing healthy cells. When tested in vivo in orthotopic mouse models of two poorprognosis cancers such as melanoma and PDAC, the compounds significantly reduced tumor size while maintaining immune and cardiac function. Involvement of ROS in the in vivo action of these drugs is indicated by prevention of their action in animals pretreated with N-acetylcysteine, an anti-oxidant. Our work provided the first direct evidence that pharmacological targeting of a mitochondrial channel leads to ROS-mediated selective apoptosis of cancer cells without causing significant side effects in vivo.
The surveys about entomological biodiversity are scarce for the argentinian Humid Chaco. In order... more The surveys about entomological biodiversity are scarce for the argentinian Humid Chaco. In order to contribute to the knowledge of the entomophauna, the transecta sampling method (100x2 m, with 5x2 m sections) was conducted at the localities of Pampa del Indio (Chaco Province) and Ibarreta (Formosa Province), during the 2006- 2007 summer, with two transects per site. The specimens were analyzed taxonomically and grouped according to their trophic habits. A total of 1,575 individuals were collected, belonging to 17 orders. Hymenoptera was, numerically, the most important order, with 63 % of the captured specimens. The highest diversity corresponded to Coleoptera, with 18 families, followed by Hymenoptera (12 families). The percentage of complimentary families between both localities was 62%. Six trophic groups recognized for insects were present..
Se analizó la macrofauna del suelo de cuatro ambientes naturales y agroecosistemas localizados en... more Se analizó la macrofauna del suelo de cuatro ambientes naturales y agroecosistemas localizados en zonas altas de la eco-región Esteros del Iberá (Corrientes, Argentina) con distintos grados de perturbación, a fin de contribuir a evaluar el impacto de las actividades productivas en este humedal. Se determinó la diversidad, abundancia, densidad y biomasa de los principales grupos y se compararon las comunidades analizadas. Se aplicó el protocolo de Conservación y Manejo Sustentable de la Biodiversidad del Suelo modificado, en cuatro puntos en cada sitio. Se analizó la influencia de distintos factores de perturbación sobre la biota. La macrofauna del suelo estuvo integrada por 19 órdenes de invertebrados, principalmente oligoquetos terrestres y artrópodos, entre los cuales las termitas, hormigas y coleópteros fueron los más abundantes. Las diferencias entre los ensambles analizados se evidenciaron en su composición taxonómica, diversidad, número de órdenes y densidad de los grupos más abundantes, pero ellas no fueron estadísticamente significativas para esta última característica. La mayoría de los individuos se detectaron en el estrato superficial del suelo (0-10 cm) y la biomasa fue más elevada en los pastizales aunque los órdenes de mayor biomasa relativa fueron distintos en cada sitio. Las similitudes entre ambientes agruparon por un lado al cultivo de algodón y al pastizal forestado, y por otro al bosque y al pastizal sometido a pastoreo. Dos de las perturbaciones ambientales analizadas (reemplazo de la vegetación natural y remoción del suelo) influyeron sobre las comunidades macroedáficas. Las hormigas y lombrices resultaron posibles invertebrados bioindicadores en estas comunidades, en tanto que las termitas subterráneas resultaron importantes por su diversidad y biomasa, principalmente en los dos ambientes con mayor grado de perturbación. Palabras clave. Invertebrados del suelo, agroecosistemas, perturbaciones antrópicas.
Background Envenomation by the South American Lonomia saturniid caterpillars, named lonomism, con... more Background Envenomation by the South American Lonomia saturniid caterpillars, named lonomism, constitutes an emerging and somewhat neglected public health issue in Argentina and neighboring countries. Considering that there is an intricate relationship between environment and human health in such cases, this study aimed to analyze the eco-epidemiological profile of 40 accidents and 33 occurrences of Lonomia spp. in Misiones (Argentina) between January 2014 and May 2020. Methodology/Principal findings We described the eco-epidemiological variables and characterized the abiotic scenario of such cases. Additionally, we obtained a density map that shows the punctual intensity of Lonomia records throughout Misiones. Most of the accidents occurred in the Department of Guaraní and involved male victims younger than 20 years old. The accidental/occasional occurrence of Lonomia spp. (considering both adult and caterpillar stages together) was significantly higher in the rural area, whereas o...
Se analizo la macrofauna del suelo de cuatro ambientes naturales y agroecosistemas localizados en... more Se analizo la macrofauna del suelo de cuatro ambientes naturales y agroecosistemas localizados en zonas altas de la eco-region Esteros del Ibera (Corrientes, Argentina) con distintos grados de perturbacion, a fin de contribuir a evaluar el impacto de las actividades productivas en este humedal. Se determino la diversidad, abundancia, densidad y biomasa de los principales grupos y se compararon las comunidades analizadas. Se aplico el protocolo de Conservacion y Manejo Sustentable de la Biodiversidad del Suelo modificado, en cuatro puntos en cada sitio. Se analizo la influencia de distintos factores de perturbacion sobre la biota. La macrofauna del suelo estuvo integrada por 19 ordenes de invertebrados, principalmente oligoquetos terrestres y artropodos, entre los cuales las termitas, hormigas y coleopteros fueron los mas abundantes. Las diferencias entre los ensambles analizados se evidenciaron en su composicion taxonomica, diversidad, numero de ordenes y densidad de los grupos mas ...
teorie informace a automatizace AVČR, v.v.i. Abstrakt: Tato práce se zabývá návrhem systematickéh... more teorie informace a automatizace AVČR, v.v.i. Abstrakt: Tato práce se zabývá návrhem systematického kombinování diskrétních pravděpodobnostních distribucí založeném na teorii rozhodování a teorii informace, konkrétně na křížové entropii (známé také jako Kullbackova-Leiblerova (KL) divergence). Optimální kombinací je pravděpodobnostní funkce minimimalizující podmíněnou střední hodnotu KL-divergence. Hustota pravděpodobnosti, která se váže k této střední hodnotě, rovněž minimalizuje KL-divergenci za podmínek vztažených křešenému problému. Ačkoliv je kombinace odvozena pro pravděpodobnostní typ informace na společném nosiči, můžeme ji po transformaci a/anebo rozšíření použít i pro míchání jiných typů informace. Práce také zahrnuje diskuzi o navrhovaném kombinování a sekvenčním zpracování dat, opakujících se datech, ovlivnění výsledků preferencemi mezi zdroji informace a aplikaci na reálná data. Klíčová slova: teorie distribuovaného rozhodování, Kullbackova-Leiblerova divergence, princip minimální křížové entropie Title: Cross-entropy based combination of discrete probability distributions for distributed decision making
Atmospheric plasma-based approaches for the degradation of dimethyl phthalate (DMP) in water
Journal of Environmental Management, 2022
Cold plasma based treatment of contaminated water is becoming a promising novel green remediation... more Cold plasma based treatment of contaminated water is becoming a promising novel green remediation option. This study assessed the performance of two different cold plasma reactors, using, respectively, a self-pulsing discharge (SPD) and a multipin corona discharge (MCD), in the degradation of dimethyl phthalate (DMP), a persistent and ubiquitous pollutant of the aquatic environment. The process kinetics and energy efficiency, as well as the main plasma generated reactive species were determined under various operating conditions concerning the plasma feed gas and flowrate, the voltage polarity, the input power, the DMP initial concentration, the liquid conductivity, and the aqueous matrix used to prepare DMP solutions for these experiments. The MCD reactor, operated with air as plasma feed gas and negative voltage polarity, gave the best results in terms of rate and energy efficiency. Moreover, variations in plasma input power and in the liquid conductivity have limited effect on DMP degradation rate, making this reactor suitable for treating liquids with a range of initial conductivities The effects of DMP initial concentration on its rate of degradation and on the process energy efficiency were also investigated. Differences in the efficiency of production and distribution of plasma generated reactive species, notably •OH and H2O2, observed for the two tested reactors are discussed in terms of different extension of the plasma/liquid interface and diffusion into the bulk solution. It is proposed that among the reactive species, •OH foremost, and O3 to a lesser extent, play a pivotal role in DMP degradation, while the contribution of H2O2 appears to be limited. The rate of DMP degradation was not drastically different in Milli-Q water and in tap water, a positive outcome in view of practical applications of the technology. The lower rate observed in tap than in Milli-Q water is attributed to the presence of bicarbonate and carbonate, which are known scavengers of hydroxyl radicals.
Objectives Positron-emission tomography (PET) has improved identification of the primary tumor as... more Objectives Positron-emission tomography (PET) has improved identification of the primary tumor as well as occult nodal burden in cancer of the head and neck. Nevertheless, there are still patients where the primary tumor cannot be located. In these situations, the standard of care is comprehensive head and neck radiation therapy however it is unclear whether this is necessary. This study examines the effects of radiation treatment volume on outcomes among using data from two cancer centers in unknown primary carcinoma of the head and neck. Methods Patients received unilateral (n = 34), or bilateral radiation (n = 28). Patient factors such as age, gender, smoking history, and patterns of failure were compared using Mann Whitney U and Chi Square. Overall survival (OS) and disease free survival (DFS) trends were estimated using Kaplan-Meier survival curves. Effect of treatment volume on survival was examined using multivariate cox proportional hazard regression model. Results No significant differences were observed in the frequency of local (p = 0.32), regional (p = 0.50), or distant (p = 0.76) failures between unilateral and bilateral radiation therapy. By Kaplan-Meier estimates, OS (3-year OS bilateral = 71.67%, unilateral = 77.90%, p = 0.50) and DFS (3-year DFS bilateral = 77.92%, unilateral = 69.43%, p = 0.63) were similar between the two treatment approaches. Lastly, multivariate analysis did not demonstrate
pero estos presentan, generalmente, un perfil nutricional poco adecuado dentro de una alimentació... more pero estos presentan, generalmente, un perfil nutricional poco adecuado dentro de una alimentación saludable. La tecnología de Fritura al vacío se presenta como una clara opción de procesamiento para el desarrollo de nuevos productos y de los ya existentes. Este artículo de revisión presenta las ventajas del uso de la Tecnología de Fritura al Vacío en diferentes tipos de alimentos, especialmente en relación al contenido de algunos micronutrientes y grasa. Se mencionan además los trabajos efectuados en Ecuador, especialmente en productos autóctonos.
BRAZILIAN JOURNAL OF AGRICULTURE - Revista de Agricultura, 2017
The study of the developmental pathways in termites contributes to the understanding of caste evo... more The study of the developmental pathways in termites contributes to the understanding of caste evolution and resources utilization, as well as other ecological aspects. In this paper, the caste developmental pathways of Cortaritermes fulviceps (Silvestri) were analyzed in natural colonies from the Province of Corrientes, Argentina. Five measurements were taken of different body parts of larvae (L), workers (W), pre-soldiers (PS), soldiers (S), nymphs (N) and alates (A) for morphometric analyses. Dissections were also performed to determine the sex of workers and soldiers. The collected data were analyzed using principal component analysis, analysis of variance and Tukey test. In the nymphal line, four nymphal instars and alates were identified. The apterous line included two larval instars. Major workers were females and minor workers, presoldiers and soldiers were males. The results suggest that the developmental pathways of C. fulviceps is similar to the one of the genus Nasutitermes Dudley.
Se estudió la influencia de técnicas de clarificación en vino de mora de Castilla Rubus glaucus B... more Se estudió la influencia de técnicas de clarificación en vino de mora de Castilla Rubus glaucus Benth sobre su cromaticidad, turbidez y rendimientos del proceso. Inicialmente con la microfiltración tangencial (MFT) a tres diferentes presiones de trabajo (1.5, 2.0 y 2.5 bar) y complementariamente con el pretratamiento de la alimentación con bentonita sódica. La MFT a 1.5 bar obtuvo el mayor Factor de Retención Volumétrica (FRV) y el mejor rendimiento, estandarizando las condiciones de presión para la MFT con pretratamiento. En función del pH, en 9 gramos se estableció la cantidad óptima de floculante añadido a cada lote de vino, llevado a reposo a 6°C (6, 12 y 24 horas), obteniéndose 610 ± 38 g para la mayor proporción de turbios en el lote de 6 horas de reposo, producto de una mayor captación inestable por parte del clarificante. La MFT de los pretratamientos indica mejoras representativas de hasta 40% en el rendimiento. Todas las muestras analizadas de vino microfiltrado con y sin ...
Revista Ecuatoriana de Medicina EUGENIO ESPEJO, 2019
El calostro bovino es una secreción mamaria que se genera aproximadamente siete días antes del pa... more El calostro bovino es una secreción mamaria que se genera aproximadamente siete días antes del parto para alimentar al becerro. El contenido de sus componentes puede llegar a ser 100 veces mayor que en la leche cruda y estudios de otros autores señalan los beneficios alimenticios y medicinales que este presenta. En tal virtud, se estudió el efecto de la aplicación de microfiltración tangencial sobre la concentración de proteínas de calostro bovino como una fuente alimenticia y medicinal. El estudio consideró vacas de la raza Holstein y sus entrecruzamientos, desarrolladas en el cantón Cayambe. Los análisis químicos (grasa, proteína total, inmunoglobulinas totales, lactosa, sólidos totales y sólidos no grasos) fueron analizados en un equipo MilkoScan, los microbiológicos (contaje de células somáticas y contaje total) en un analizador Bacto Scan, la cuantificación de inmunoglobulina G con un cromatógrafo líquido Varian y la influencia de la aplicación de microfiltración tangencial con...
This paper reports the characterization of a low-power, atmospheric pressure plasma source develo... more This paper reports the characterization of a low-power, atmospheric pressure plasma source developed for and successfully tested in applications of plasma medicine. The source produces plasma by ionizing a helium flow containing traces of air. The effluent gas is thus enriched in reactive oxygen and nitrogen species as well as helium metastable excited states. Electrical measurements, Schlieren imaging, OES and various chemical analyses and probes enabled us to fully characterize the plasma source and to determine some fundamental parameters for treating living samples such as the power feeding the discharge, the optimal source to target distance, the maximum exposure time, and to identify the major chemical species responsible for the biological action on exposed living matter. K E Y W O R D S atmospheric plasma jet, optical emission spectroscopy, plasma medicine, reactive oxygen species, Schlieren 1 | INTRODUCTION Plasma medicine is the emerging discipline dealing with the use of cold, atmospheric pressure plasmas as a tool to interact with living matter for therapeutic purposes. [1-4] The basic requirement for plasma sources to be applicable in this field is to produce a diffuse plasma at atmospheric pressure with power levels low enough to keep the treated substrate temperature below 37°C, which means typically a few Watts of dissipated power. The most used process gases are helium and argon, mixed with small fractions of oxygen, nitrogen, Gabriele Neretti and Francesco Tampieri share the first authorship.
The potassium channel Kv1.3 is highly expressed in the mitochondria of various cancerous cells. H... more The potassium channel Kv1.3 is highly expressed in the mitochondria of various cancerous cells. Here we show that direct inhibition of Kv1.3 using two mitochondria-targeted inhibitors alters mitochondrial function and leads to reactive oxygen species (ROS)-mediated death of even chemoresistant cells independently of p53 status. These inhibitors killed 98% of ex vivo primary chronic B-lymphocytic leukemia tumor cells while sparing healthy B cells. In orthotopic mouse models of melanoma and pancreatic ductal adenocarcinoma, the compounds reduced tumor size by more than 90% and 60%, respectively, while sparing immune and cardiac functions. Our work provides direct evidence that specific pharmacological targeting of a mitochondrial potassium channel can lead to ROS-mediated selective apoptosis of cancer cells in vivo, without causing significant side effects.
The European Physical Journal Applied Physics, 2016
We are pleased to introduce this special issue which collects a selection of contributions to the... more We are pleased to introduce this special issue which collects a selection of contributions to the sixth Central European Symposium on Plasma Chemistry, CESPC-6, held in Bressanone, Italy, during September 6-10, 2015. The conference, which received support by CEI-the Central European Initiative, gathered and provided a lively forum for scientists, including many PhD students and young post-docs, from 20 different countries and 4 continents. The scientific focus of the conference was on major areas of fundamental studies and applications of plasma, and included: fundamentals, modelling and diagnostics, new materials, energy technologies, environmental protection, bio-and medical-plasma technologies, surface processes. This selection of papers is well representative for the conference scientific contents. We would like to thank all the Authors and Referees for their efforts in preparing and reviewing the manuscripts, as well as the editorial office of the EPJAP for its helpful assistance in organizing this special issue.
Biochimica et Biophysica Acta (BBA) - Bioenergetics, 2016
Mitochondria are involved in cell signalling, proliferation, differentiation and apoptosis. Their... more Mitochondria are involved in cell signalling, proliferation, differentiation and apoptosis. Their participation in these processes depends on the production of ATP and ROS, both generated by electron transfer reactions. Bioenergetics has become central to our understanding of pathological mechanisms as well as for the development of new therapeutic strategies and as a tool for gauging disease progression in cancer, neurodegeneration, diabetes and cardiovascular disease. The view is emerging that mitochondrial inner membrane potassium channels have a profound effect on organelle function and, consequently, on the survival of the whole cell. Recently, we provided evidence that direct inhibition of the mitochondrial Kv1.3 channel by two novel mitochondria-targeted derivatives of PAP-1, a specific inhibitor of Kv1.3, induces apoptosis. Direct actions of these drugs on mitochondria are indicated by immediate swelling, loss of mitochondrial membrane potential, release of reactive oxygen species (ROS) and altered respiration. Both drugs efficiently kill melanoma and pancreatic ductal adenocarcinoma (PDAC) cancer cells in vitro in the microMolar range. Furthermore, they induced apoptosis in ex-vivo primary tumor cells from chronic B-lymphocytic leukemia, while sparing healthy cells. When tested in vivo in orthotopic mouse models of two poorprognosis cancers such as melanoma and PDAC, the compounds significantly reduced tumor size while maintaining immune and cardiac function. Involvement of ROS in the in vivo action of these drugs is indicated by prevention of their action in animals pretreated with N-acetylcysteine, an anti-oxidant. Our work provided the first direct evidence that pharmacological targeting of a mitochondrial channel leads to ROS-mediated selective apoptosis of cancer cells without causing significant side effects in vivo.
The surveys about entomological biodiversity are scarce for the argentinian Humid Chaco. In order... more The surveys about entomological biodiversity are scarce for the argentinian Humid Chaco. In order to contribute to the knowledge of the entomophauna, the transecta sampling method (100x2 m, with 5x2 m sections) was conducted at the localities of Pampa del Indio (Chaco Province) and Ibarreta (Formosa Province), during the 2006- 2007 summer, with two transects per site. The specimens were analyzed taxonomically and grouped according to their trophic habits. A total of 1,575 individuals were collected, belonging to 17 orders. Hymenoptera was, numerically, the most important order, with 63 % of the captured specimens. The highest diversity corresponded to Coleoptera, with 18 families, followed by Hymenoptera (12 families). The percentage of complimentary families between both localities was 62%. Six trophic groups recognized for insects were present..
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Papers by Manuel Coronel