Skip to content

Not able to edit .hic file in Juicebox #958

@sgr308

Description

@sgr308

Hi,
I assembled a genome assembly using SALSA2 and generated a .hic file using this method:
https://github.com/marbl/SALSA#generate-hic-file-from-salsa-scaffolds

Then, I generated .agp and .assembly files using "makeAgpFromFasta.py" and "agp2assembly.py" from juicebox_scripts.
https://github.com/phasegenomics/juicebox_scripts/tree/master/juicebox_scripts

First, I loaded .hic file and then I loaded .assembly file in Juicebox_1.11.08. The upload of both files completed without any error and I can see the Edit, Chr and Scaf on the right side but I am not getting the blue, yellow and green lines which I can see in this video at 2:00 minutes (https://www.youtube.com/watch?v=Nj7RhQZHM18).

The current .hic shows 21 scaffolds while I am expecting 19 scaffolds as this species contains 19 chromosomes. I can provide you the .hic and .assembly file for any troubleshoot.

Thanks,
Sagar

Metadata

Metadata

Assignees

No one assigned

    Labels

    No labels
    No labels

    Type

    No type

    Projects

    No projects

    Milestone

    No milestone

    Relationships

    None yet

    Development

    No branches or pull requests

    Issue actions