Hi,
I assembled a genome assembly using SALSA2 and generated a .hic file using this method:
https://github.com/marbl/SALSA#generate-hic-file-from-salsa-scaffolds
Then, I generated .agp and .assembly files using "makeAgpFromFasta.py" and "agp2assembly.py" from juicebox_scripts.
https://github.com/phasegenomics/juicebox_scripts/tree/master/juicebox_scripts
First, I loaded .hic file and then I loaded .assembly file in Juicebox_1.11.08. The upload of both files completed without any error and I can see the Edit, Chr and Scaf on the right side but I am not getting the blue, yellow and green lines which I can see in this video at 2:00 minutes (https://www.youtube.com/watch?v=Nj7RhQZHM18).
The current .hic shows 21 scaffolds while I am expecting 19 scaffolds as this species contains 19 chromosomes. I can provide you the .hic and .assembly file for any troubleshoot.
Thanks,
Sagar
Hi,
I assembled a genome assembly using SALSA2 and generated a .hic file using this method:
https://github.com/marbl/SALSA#generate-hic-file-from-salsa-scaffolds
Then, I generated .agp and .assembly files using "makeAgpFromFasta.py" and "agp2assembly.py" from juicebox_scripts.
https://github.com/phasegenomics/juicebox_scripts/tree/master/juicebox_scripts
First, I loaded .hic file and then I loaded .assembly file in Juicebox_1.11.08. The upload of both files completed without any error and I can see the Edit, Chr and Scaf on the right side but I am not getting the blue, yellow and green lines which I can see in this video at 2:00 minutes (https://www.youtube.com/watch?v=Nj7RhQZHM18).
The current .hic shows 21 scaffolds while I am expecting 19 scaffolds as this species contains 19 chromosomes. I can provide you the .hic and .assembly file for any troubleshoot.
Thanks,
Sagar