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Miniscope Protocol

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Jess edited this page Jan 27, 2025 · 5 revisions

So you want to do single photon calcium imaging? Well here's the guide for you:

(under construction)

Supplies

  • General surgery supplies
  • GRIN lens forceps (made of plastic, not metal!!)
  • Torq screwdriver for baseplate set screw
  • Lens paper
  • 1mm diameter (or smaller) heat shrink for holding GRIN lens in stereotaxic holder
  • GRIN lens
  • miniscope baseplate with cover

Surgery

Overall surgery protocol, from the UCLA Miniscope wiki can be found here.

A protocol that, in my opinion, is in-depth and includes images that I found to be helpful is from CP Neuroscience - 2018 - Zhang - Miniscope GRIN Lens System for Calcium Imaging of Neuronal Activity from Deep Brain.pdf

Imaging

🧠 Home

📘 General Information

EHS and DCM procedures

🐭 Animal policies

🧪 Experimental Protocols

Weighing mice and food

✂️ Intracranial Surgical Protocols

✂️ Other Surgical Protocols

🩸 Blood collection

🍬 Glucose tolerance testing

Intracranial injections

🧬 Biomolecular protocols

Von Frey testing

🧬 Virus Injections

💡 Photometry

Acquisition

Analysis

In vivo electrophysiology

🧪 Pharmacology

Behavior Chambers

Bonsai workflows

🔬 Histology

Microscopy

Devices

🛠️ Arduino

FED3

Sippers

Tumble Feeder

Social Door

📊 Data Analysis

Statistical Power

Von frey analysis

FED3 analyses

BEAM analyses

Electrophysiology

Ex Vivo

In Vivo

🎥 Imaging

Miniscopes

1P Imaging with Mightex OASIS

🧱 Miscellaneous Tools

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