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HumanBandit
Kravitz Lab edited this page Nov 17, 2025
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For human studies we use a modified code and plastic beads.
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Flash this code on FED3.
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Print modified nose piece and glue that on.
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Fill FED3 with 3mm silver plastic beads
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- Onboarding Guide
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- NRB Printer setup
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- Software Setup
- Poster Printing
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- Reviewing Papers
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- Onboarding WashU Undergraduates
- Cage cards
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- Surgery cards
- Mouse Transfer Procedure
- Cage Check
- IACUC Protocols
- Mouse Genotyping
- Autoclave
- Diet nutritional information
-- Chow diet
-- FED3 grain pellets (Bioserv)
-- FED3 chocolate crispies (ViaCheff)
-- High fat diet
- RNA extraction (Trizol)
- cDNA synthesis with Biorad reagent
- Designing primers
- Setting up the qPCR and analysis
- Localizing injections with DiI
- GCaMP8
- ChR2 (H134R)
- GrabDA
- dLight
- Caspase
- mCherry / YFP Controls
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Acquisition
Analysis
- Electrode array construction
- Attaching fibers to Inn NeuroPhys arrays
- NanoZ Impedance Testing
- NanoZ Gold Plating
- Recording Setup
- Data Analysis
- Electrode lesions for histology
- Antibody stocks
- Cryostat
- Freezing microtome
- SST & PV immunostaining
- GFP immunostraining
- IHF troubleshooting
- FED3 Troubleshooting
- FED3 Human Bandit Code
- Synchronizing FED3 with recordings
- FED3 Bandit Code for recordings
- FED3 Artwork
Ex Vivo
- Overview
- Current Clamp
- Voltage Clamp
- Clampex Protocols
- Solutions & Recipes
- Troubleshooting
- Post-patching IHC
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In Vivo