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ChR2.md

im-jwang edited this page Apr 21, 2025 · 3 revisions

image

Injection stereotaxic targeting: (VP) AP: 0.2, ML: 1.5, DV: -4.9

Virus construct: AAV5-eF1a-DIO-ChR2(H134R)-eYFP, titer: 1:4 dilution 6E10^12 vg/mL https://www.addgene.org/20298/

Injection volume: 300 nL, 50nL/60sec per cycle

Signal enhanced with 1:1000 chicken anti-GFP primary ab, 1:500 anti-chicken fluorescein secondary ab

🧠 Home


πŸ“˜ General Information

EHS and DCM procedures

🐭 Animal policies


πŸ§ͺ Experimental Protocols

Weighing mice and food

βœ‚οΈ Intracranial Surgical Protocols

βœ‚οΈ Other Surgical Protocols

🩸 Blood collection

🍬 Glucose tolerance testing

Intracranial injections

🧬 Biomolecular protocols

Von Frey testing

🧬 Virus Injections

πŸ’‘ Photometry

Acquisition

Analysis

In vivo electrophysiology

πŸ§ͺ Pharmacology

Behavior Chambers

Bonsai workflows

πŸ”¬ Histology

Microscopy


Devices

πŸ› οΈ Arduino

Sippers

Tumble Feeder

Social Door


πŸ“Š Data Analysis

Electrophysiology

Ex Vivo

In Vivo


πŸŽ₯ Imaging

Miniscopes

1P Imaging with Mightex OASIS


🧱 Miscellaneous Tools

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