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Date : October 1, 2009

ljlksa rFkk dju jkbZ

¼czSfldk tqafl;k ,y- LtuZ rFkk dkWl ,oa
czSfldk dSfjukVk ,- czkmu½
ij
fof'k"Vrk] ,d:irk rFkk LFkkf;Ro
ijh{k.k ds fy,
fn'kkfunsZf'kdk
Guidelines
for the Conduct of Test for
Distinctiveness, Uniformity and Stability
On
Indian Mustard & Karan Rai
(Brassica juncea L. Czern & Coss and
Brassica carinata A Braun)

ikS/kk fdLe vkSj Ñ"kd vf/kdkj laj{k.k izkf/kdj.k

Protection of Plant Varieties and Farmers' Rights Authority
(PPV & FRA)
Hkkjr ljdkj
Government of India

4
 2

ljlksa ¼czSfldk tqafl;k ,y- LtuZ ,oa dkWl½ rFkk dju jkbZ
¼czSfldk dSfjukVk ,- czkmu½
I. fo"k;
ijh{k.k ds ;s fn'kkfuns'Z k ljlksa ¼czSfldk tqafl;k ,y- LtuZ ,oa dkWl½ rFkk dju
jkbZ ¼czSf ldk dSfjukVk ,- czkmu½ dh leLr fdLeksa] la djks]a ijkthfu;ksa rFkk iS r`d
oa'kØeksa ij ykxw gksx
a sA

II. visf{kr lkexzh

1- ikS/kk fdLe ,oa d`"kd vf/kdkj laj{k.k vf/kfu;e ¼ihihoh ,oa ,Qvkj vf/kfu;e½
2001 ds rgr ia thdj.k ds fy, fdLe dk uke j[kus la ca/kh ijh{k.k esa vuqiz;ksx ds
fy, t#jh cht lkexzh dh ek=k vkSj [Link]Ùkk fdruh] dgka vkSj dc gksxh bldk
[Link]; ikS/kk fdLe vkSj d`"kd vf/kdkj laj{k.k izkf/kdj.k ¼ihihoh ,oa ,Qvkj,½ }kjk
fd;k tk,xkA vkosnd }kjk Hkkjr ds vykok fdlh Hkh vU; ns'k dh bl iz dkj dh
cht lkexzh dks izL rq r djrs le; ;g lqfuf'pr fd;k tk,xk fd lacaf/kr ns'k ds
dkuwu ,oa fofu;eksa ds rgr lhek 'kqYd vkSj laxjks/k laca/kh fu/kkZfjr vko';drkvksa
dk ikyu fd;k x;k gSA vkosnd }kjk iz nku dh tkus okyh cht dh U;wure ek=k
izR;k'kh fdLe ;k ladj ds ekeys esa 500 xzk - rFkk ladj ds iSr` d oa'kØe ds ekeys
esa 250 xzk - gksxhA bu chtksa dh izR;sd ykWV dks iSd] lhycan o mfpr iz dkj ls
yscyhd`r fd;k tk,xk vkSj blds 10 leku Hkkj okys iSdsV cuk, tk,axs rFkk bUgsa
,d ykWV esa izLrqr fd;k tk,xkA iS r`d oa'kØeksa dks ,d iS dsV esa iS d fd;k
tk,xkA
2- izLrq r fd, x, cht esa de ls de 85 izfr'kr va dqj.k] 98 izfr'kr HkkSfrd 'kq)rk]
loksPZ p vkuqoaf'kd 'kq) rk] le#irk] LoPNrk vkSj ikni LoPNrk laca/kh ekud gksus
pkfg,A blds vfrfjDr HkaMkj.k laca/kh lqj{kk dh vko';drkvksa dks iwjk djus ds
fy, cht esa ueh dh ek=k 8 izfr'kr ls vf/kd ugha gksuh pkfg,A vkosnd dks cht
 3

ds lkFk&lkFk izLrq rhdj.k dh frfFk ls vf/kd ls vf/kd ,d ekg dh vof/k ds

nkSjku fd, x, va dqj.k ijh{k.k ds [Link] vkadM+s izLrqr djus pkfg,A
3- cht lkexzh dk fdlh Hkh izdkj ds jklk;fud vFkok tSoHkkSfrd mipkj u fd;k
tk,A

III. ijh{k.k djuk

1- Mh;w,l ijh{k.k dh U;wure vof/k lkekU;r% de ls de nks Lora= leku o`f) pØ
gksxhA
2- ijh{k.k lkekU; rkSj ij de ls de nks LFkkuksa ij fd;k tkuk pkfg,A ;fn bu
LFkkuksa ij ns[kus ls izR;k'kh fdLe dk dksbZ vfuok;Z xq.k n`f"Vxkspj u gks] rks fdLe
dh fdlh vU; mi;qDr ijh{k.k LFky ij tkap dh tkuh pkfg, vFkok vkosnd ds
vuqjks/k ij fo'ks"k ijh{k.k izksVksdkWy viuk, tkus pkfg,A
3- [ksr ijh{k.k Qly dh lkekU; c<+okj laca/kh vuqdwy fLFkfr;ksa vkSj leLr ijh{k.k
fof'k"Vrkvksa dh vfHkO;atdrk ds rgr fd, tk,aA IykWVksa dk vkdkj bruk gksuk
pkfg, fd ikS/kksa dks ;k ikS/kksa ds Hkkxksa dks ekiu vkSj i;Zos{k.k ds fy, [kM+s ikS /kksa ds
i;Zos{k.k laca/kh fcuk fdlh iw okZxzg ds IykWV ls vklkuh ls fudkyk tk lds vkSj
,slk ikS/kksa ;k Qly dh c<+okj dh vafre voLFkk rd fd;k tk lds A izR;sd
ijh{k.k esa yxHkx 700 ikS/ks fy, tk,axs A buds fy, IykWV dk vkdkj vkSj jksikbZ
varjky rhuksa izfrd`f r;ksa esa fuEu fof'k"Vrk ds vuq lkj j[kk tk,xkA i;Zos{k.k vkSj
ekiu ds fy, vyx IykWV dk bLrseky rHkh fd;k tk ldrk gS tc muds fy,
,d leku i;kZoj.k fLFkfr;ka j[kh xbZ gksAa lHkh izfrd`fr;ksa ds fy, ijh{k.k LFky
dh ,d leku i;[Link]; fLFkfr;ka gksuh pkfg,A
4- ijh{k.k IykWV fMtkbu %
drkjksa dh la[;k % 6
drkj yackbZ % 6 eh-
drkj ls drkj dh nwjh % 45 ls-a eh-
 4

ikS/ks ls ikS/ks dh nwjh % 15 ls-a eh-

ikS/kksa dh dqy visf{kr la[;k % 720
izfrd`f r;kas dh la[;k % 3

5- esM+ ds ikl dh drkjksa okys ikS/kksa ds i;Z os{k.k fjdkWMZ ugha fd, tkus pkfg,A
6- ihihoh ,oa ,Qvkj izkf/kdj.k fo'ks"k ijh{k.k ds fy, vfrfjDr ijh{k.k izksVksdkWy
fu/kkZfjr djsxkA

IV. fof/k;ka vkSj i;Zos{k.k

1- [Link] dh rkfydk ¼vuqHkkx VII ns[ks½a esa [Link] [Link] dk mi;ksx Mh;w,l ds fy,
fdLeksa rFkk ladjksa ds ijh{k.k gsrq fd;k tk,xkA
2- fof'k"Vrk vkSj LFkkf;Ro ds ewY;kadu ds fy, de ls de 60 ikS/kksa ;k 60 ikS/kksa ds
Hkkxksa ls i;Zos{k.k fd, tk,axs vkSj ftUgsa 3 leku izfrd`fr;ksa esa ckaVk tk,xk ¼izR;sd
izfrd`f r 20 ikS/ks½A
3- [Link] dh le:irk ds ewY;kadu ds fy, [Link] IykWV ¼ikS/kksa ds lewgksa ;k ikS/kksa ds
Hkkxksa ds ,d i;Zos{k.k }kjk n`"VO; ewY;kadu ds fy,½ 2 izfr'kr ds tula[;k ekud
ds iSr `d oa'kØeksa dks fy;k tk,xkA bldh Lohdk;Zrk laHkkO;rk fdLeksa vkSj ladjksa
ds fy, de ls de 95 izfr'kr rFkk tula[;k ekud 5 izfr'kr ds lkFk de ls
de 95 izfr'kr Lohdk;Z laHkkO;rk gksuh pkfg,A 700 ikS/kksa ds uew uk vkdkj ds
ekeys esa vkWQ Vkbiksa dh la[;k iSr` d oa'kØeksa esa 10 izfr'kr vkSj fdLeksa o la djksa
ds ekeys esa 50 izfr'kr ls vf/kd ugha gksuh pkfg,A
4- jax laca/kh [Link] ds ewY;kadu ds fy, jkW;y gkWVhZdYpjy lkslk;Vh ¼vkj,p,l½
uohure jax ds pkVZ dk mi;ksx fd;k tk,A
5- tc rd vU;Fkk u bafxr fd;k x;k gks] iÙkh ds lHkh i;Zos{k.k [Link] fodflr ifÙk;ksa
ij dfydk cuus vkSj iq" i fudyus ds chp dh vof/k esa dh tkuh pkfg,A
 5

V. fdLeksa dk lewghdj.k
1- fof'k"Vrkvksa ds ewY;kadu esa lqfo/kk ds fy, Mh;w,l ijh{k.k gsrq izR;k'kh fdLeksa dks
lewgksa esa ckaVk tk,xkA os xq.k tks vuqHko ls Kkr fd, x, gkssx
a s vkSj fHkUu ugha gksx
a s
vFkok ,d fdLe es a cgqr de fHkUu gksx
a s rFkk tks [Link] fdLeksa esa viuh fofHkUu
voLFkkvksa esa leku :i ls O;kIr gksx
a s] lewghdj.k ds mn~ns'; ls mi;qDr ekus
tk,axAs

2- ljlksa vkSj dju jkbZ dh fdLeksa ds lewghdj.k ds fy, fuEu [Link] dk mi;ksx fd;k
tk,xk%
i½ iÙkh % yksc dh la[;k ¼xq.k 4½
ii½ iq"i % iq" iu dk le; ¼xq.k 8½
iii½ ikS/kk % eq[; izjksg dh yackbZ ¼xq.k 12½
iv½ Qyh % izfr Qyh chtksa dh la[;k ¼xq.k 20½

VI. xq.k vkSj fpg~u

1- fof'k"Vrk] ,d:irk rFkk LFkkf;Ro dk vkdyu djus ds fy, xq.k rkfydk ¼vuqHkkx
VII ½ esa fn, x, [Link] vkSj mudh voLFkkvksa dk bLrseky fd;k tk,A
2- fMftVy Ms Vk izks lsflax ds iz;kstu gsrq fofHkUu [Link] dh vfHkO;fDr dh izR;sd
voLFkk gsrq fVIif.k;ksa ¼1 ls 9½ dk mi;ksx fd;k tk, A
3- 'kh"kZd %
(*) izR;sd c<+ okj ekSle esa lHkh ijh{[Link]/khu fdLeksa ds i;Zosf{kr [Link] dk mi;ksx
fdLeksa ds fooj.k esa 'kkfey fd;k tkuk pkfg,A bldk viokn rHkh gks tc iw oZ
[Link] dh vfHkO;fDr] ijh{k.k {ks= dh i;[Link]; fLFkfr;ksa ;k iwoZorhZ lekaxh [Link]
}kjk laHko u gksA viokn dh ,s lh fLFkfr esa mfpr Li"Vhdj.k fn;k tkuk pkfg,A
 6

(+) vuqHkkx VIII esa fn, x, [Link] dh O;k[;k ns[ksAa ;g uksV fd;k tk, fd dqN [Link]
ds fy, ikS/ks ds ftu Hkkxksa dk i;Zos{k.k fd;k tkuk gS mudk fooj.k Li"Vrk gsrq
O;k[;k ;k fp= ¼fp=ks½a }kjk fd;k x;k gS u fd jax laca/kh fofo/krk n'kkZus ds
fy,A
4- ikS/ks dh o`f) vkSj c<+okj ds nkSjku izR;sd xq.k ds i;Zos{k.k ds fy, b"Vre voLFkk
dks [Link] dh rkfydk ds lkrosa dkWye esa n'keyo dksM la[;k ls n'kkZ;k x;k gSA
bu n'keyo dksM la[;kvksa ls lEc) c<+ okj voLFkkvksa dk [Link] fuEukuq lkj gS %
c<+okj voLFkkvksa ds fy, n'keyo dksM
dksM c<+ okj voLFkk
00 'kq"d cht
50 dyh f[kyuk
60 Qwy fudyuk
62 vafre Nksj ij dq N f[kyh dfy;ka
79 vafre Nksj ij Qyh ds lHkh cht xgjs jax ds
85 ifjiDork
90 Åijh Qyh esa chtksa esa Hkwjs {ks=
100 dVkbZ ds i'pkr

5- xq.k&rkfydk ds dkWye 8 esa fn;s x, xq. kksa ds ewY;kadu dk izdkj fuEukuqlkj gS %

,eth % ikS/ks ds lewg ;k ikS/ks ds fdlh Hkkx dh ,dy i;Zos{k.k }kjk eki

,e,l % vusd ,dy ikS/kksa ;k ikS/kksa ds fdlh Hkkx dh eki

ikS/ks ds lewgksa ;k ikS/kksa ds fdlh Hkkx dk ,dy i;Zos{k.k }kjk n`f"Vxr
ohth %
ewY;kadu
oh,l % ,dy ikS/ks ;k ikS/kksa ds fdlh Hkkx dk i;Zos{k.k }kjk n`f"Vxr ewY;kadu
 7

VII. [Link] dh rkfydk

Ø- xq.k voLFkk [Link] mnkgj.k fdLesa i;Zos{k.k dh ewY;kadu
la- voLFkk dk izdkj
ch-tqa fl;k ch- dSfjukVk ¼dksM la-½
1 2 3 4 5 6 7 8
1. iÙkh % jksfeyrk vuqifLFkr 1 clarh] vkj,p 781 fdju] ihlh5 50-60 oh,l
(+) fojy 3 o:.k] iwlk cksYM -
l?ku 5 lh,l 52] xhrk -
2. iÙkh % jax gYdk gjk 1 ,uMhvkjbZ 4 - 50-60 ohth
(*) e/;e gjk 2 o:.k] chvkbZvks 902 -
xgjk gjk 3 th,e 1 fdju] ihlh5
3. iÙkh % ikfy vuqifLFkr 1 - - 50-60 oh,l
(*) mifLFkr 9 o:.k fdju] ihlh5
(+)
4. iÙkh % ikfy;ksa dh vYi ( < 5) 3 - - 50-60 ,e,l
(*) la[;k e/;e 5 Økafr ihlh 5
(6 – <8)
vf/kd (> 8) 7 lh,l 52] vkj,p fdju
819
5. iÙkh % dksjksa ij [Link] 1 - fdju] ihlh 50-60 oh,l
(*) [kkaps 5
(+) nkarq,nkj 2 o:.k] chvkbZvks 902 -
narqj 3 ,uMhvkjbZ 4
6. iÙkh % yackbZ ¼lsa- ch- tqafl;k
eh-½ NksVh ( < 25 ) 3 ,uMhvkjbZ 4 50-60 ,e,l
e>ksyh 5 o:.k
(26 – <30 )
yach (>30 ) 7 vkj,p 781]
ihlhvkj 7
ch- dSfjukVk
NksVh 3 - 50-60 ,e,l
( < 30 )
e>ksyh 5 ihlh 5
(31-<35)
yach (> 35 ) 7 fdju
7. iÙkh % pkSMk+ bZ ¼lsa- ladjh (< 10) 3 ,uMhvkjbZ 4 - 50-60 ,e,l
eh-½
e>ksyh 5 o:.k] th,e 1 ihlh 5
(10 –12)
pkSMh+ 7 vkj,p 781] fdju
(> 12 ) ihlhvkj 7
 8

8. iq"i % iq"iu dk ch- tqfal;k

le; ¼de ls vxsrh 3 ,uMhvkjbZ 4 60-62 ,eth
(*) de ,d f[kys ( <40 fnu)
Qwy okys 50 % e/;e 5 chvkbZvks 902] th,e
ikS/ks½ (41 - <50 1
fnu)
iNsrh 7 vkj,p 8113
(> 50 fnu)
ch- dSfjukVk
vxsrh 3 - 60-62 ,eth
(< 50 fnu)
e/;e 5 ihlh 5
(51 – <60
fnu)
iNsrh 7 fdju
(> 60 fnu)
9. iq"i % ia[kqMh+ dk lQsn 1 - - 60-62 ohth
(*) jax gYdk ihyk 2 iwlk egd -
ihyk 3 o:.k] chvkbZvks 902 fdju] ihlh5
ukjaxh 4 - -
10. iq"i % ia[kqMh+ dh NksVh (<1.2 ) 3 ,uMhvkjbZ 4 - 60-62 ,e,l
yackbZ ¼lsa-eh-½ e>ksyh 5 iwlk cksYM] [Link] fdju
(1.2-1.5 )
yach (>1.5 ) 7 - ihlh 5
11. iq"i % ia[kqMh+ dh ladjh(<0.6) 3 - - 60-62 ,e,l
pkSMk+ bZ ¼lsa-eh-½ e/;e 5 clarh] chvkbZvks 902 -
(0.6-0.7 )
pkSMh+ (>0.7 ) 7 vkj,y 1359 fdju] ihlh5

12. ikS/kk % eq[; izjksg NksVk 3 - fdju 79 ,e,l

(*) dh yackbZ ¼lsa-eh-½ ( < 40 )
e>ksyk 5 vkjlhlh 4 tsVhlh 1
(41- <50 )
yack 7 th,e 1 -
(51 - <60 )
cgqr yack 9 [Link]] xhrk -
(>60 )
13. ikS/kk % ÅapkbZ ¼lsa- NksVk (< 130 3 ,uMhvkjbZ 4 - 79 ,e,l
(*) eh-½ )
e>ksyk 5 ,l- ,lSd -
(131- <150)
 9

yack 7 o:.k] iwlk cksYM fdju

(151 - <170
)
cgqr yack 9 clarh] vkj,p 819 ihlh 5
(> 170 )
14. Qyh % yackbZ NksVh 3 dkafr fdju 85 ,e,l
(*) ¼ls-a eh-½ ( < 4.5 )
(+) e>ksyh 5 vkj,p 30 ihlh 5
(4.5-5.5 )
yach 7 iwlk cksYM -
(> 5.5 )
15. Qyh % uksd
a dh NksVh 3 xhrk ihlh 5 85 ,e,l
yackbZ (<0.8 )
e>ksyh 5 ihchvkj 97] -
(0.8 – <1.2 ) ihlhvkj 7
yach 7 iwlk cgkj -
(> 1.2 )
16. Qyh % eq[; cgqr de 3 ,uMhvkjbZ 4 fdju] ihlh 85 ,e,l
(*) izjksg dh la[;k (<40) 5
de (41 - 5 o:.k tsVhlh 1
<50)
e/;e 7 [Link] -
(51 - <60)
vusd (> 60) 9 xhrk -
17. Qyh % eq[; de (<0.7) 3 ,uMhvkjbZ 4 ihlh 5 85 ,e,l
(+) izjksg ij ?kuRo e/;e 5 o:.k -
(0.7 - 0.8)
mPp (>0.8) 7 - fdju
18. Qyh % eq[; ladjk 1 lUtDVk ,lSd iwlk [Link] 85 ohth
(*) izjksg dk dks.k v/kZ ladjk 2 [Link]] xhrk ihlh 5
(+)
[kqyk gqvk 3 o:.k fdju
19. Qyh % cukoV fpduh 3 - - 85 ohth
(*) [kqjnjh 5 o:.k -
(+) ladqfpr 7 clarh fdju] ihlh5
20. Qyh % izfr cgqr de 3 - - 85 ,e,l
(*) Qyh chtksa dh ( <12)
la[;k de(13-<16) 5 th,e 1] o:.k ihlh 5
e/;e(17- 7 lh,l 52] lst&2 -
<20)
vusd (> 20) 9 xhrk -
21. ifjiDork vof/k ch- tqafl;k
 10

(*) vxsrh 3 ,uMhvkjbZ 4 90 ,eth

(+) ( <110 fnu)
e/;e 5 lst 2
(111 -<130
fnu)
iNsrh 7 o:.k] [Link]
(131-<150
fnu)
vfr iNsrh 9 -
(> 150 fnu)
ch- dSfjukVk
vxsrh (<120 3 - 90 ,eth
fnu)
e/;e (120 - 5 ihlh 5
<140 fnu)
iNsrh (140- 7 fdju
<160 fnu)
vfr iNsrh 9 -
(> 160 fnu)
22. cht % cht dk ihyk 1 clarh fdju 100 ohth
(*) jax ykfyek;qDr 2 lh,l 52 ihlh 5
Hkwjk
Hkwjk 3 vkjlhlh 4 -
xgjk Hkwjk 4 chvkbZvks 902 -
dkyk 5 - -
23. cht % vkdkj ch- tqafl;k
(*) ¼1000 chtksa dk NksVk (<5.0 3 Økafr 100 ,eth
Hkkj½ xzk-)
e>ksyk 5 [Link]
(5.0-6.0 xzk-)
eksVk (>6.0 7 iwlk cksYM
xzk-)
ch- dSfjukVk
NksVk (<4.0 3 - 100 ,eth
xzk-)
e>ksyk 5 fdju] ihlh
(4.0-6.0 xzk-) 5
eksVk (>6.0 7 -
xzk-)
24. vYi (<38) 3 - ihlh 5 100 MG
(*) cht % rsy va'k e/;e 5 lh,l 52] o:.k fdju
(+) ¼%½ (38 – <42 )
 11

mPp (42- 7 [Link] -

46)
vfr mPp 9 - -
(>46)

VIII. xq.k rkfydk dh O;k[;k

xq.k 1- iÙkh % jksfeyrk
iÙkh jksfeyrk esa iÙkh dh fupyh lrg dk i;Zos{k.k fd;k tkuk pkfg,A

1 3 5
vuqifLFkr fojy l?ku

xq.k 3- iÙkh % ikfy

ikfy;ksa dh vuqifLFkfr ;k mifLFkfr dk i;Zos{k.k dyh cuus ls iq" i fudyus
dh voLFkk ds chp [Link] fodflr iÙkh ij fd;k tkuk pkfg,A i=ny ds Hkkxksa dks
rc ikfy ekuk tkrk gS tc mudh yackbZ iÙkh ds tqM+ko fcanq ls iÙkh ds MaBy dh
pkSM+kbZ ds de ls de cjkcj gks vkSj i=ny ds Åijh Nksj dh yackbZ ikfy dh yackbZ
dh de ls de vk/kh gksA f}rh;d ikfy ¼ikfy;ks½a dh [Link] ugha dh tkrh gSA
 12

xq.k 5- iÙkh % dksjksa ds [kkapsa

dksjksa ds [kkapksa dk i;Zos{k.k i=ny ds Åijh ,d frgkbZ Hkkx ij fd;k tkuk pkfg,A

1 2 3
[Link] nkarq,nkj narqj

xq.k 14- Qyh % yackbZ

Qyh dh yackbZ blds MaBy ls uksd rd gksrh gSA bldk vkdyu eq[; izjksg ds
fupys ,d frgkbZ Hkkx ls fd;k tkuk pkfg,A

xq.k 17- Qyh % eq[; izjksg dk ?kuRo

bldh [Link] eq[; izjksg ij yxh Qfy;ksa dh la[;k rFkk eq[; izjksg dh yackbZ ds
vuqikr ds vk/kkj ij dh tkuh pkfg,A

xq.k 18- Qyh % eq[; izjksg dk dks.k

Qyh dk dks.k eq[; izjksg rFkk eq[; izjksg ds fupys ,d frgkbZ Hkkx ij ekStwn MaBy
ds chp dk dks.k ukirs gq, fd;k tkuk pkfg,A
 13

1 2 3
ladjh v/kZ&ladjh [kqys gq,

xq.k 19- Qyh % cukoV

3 5 7
fpduh [kqjnjh ladqfpr

xq.k 21- ifjiDork vof/k

ifjiDork dh vof/k dks cqvkbZ dh frfFk ls ml fnu rd fjdkWMZ fd;k tkuk pkfg,
tc 75 izfr'kr Qfy;ka id dj ihyh iM+ tk,aA
 14

xq.k 24- cht % rsy va'k

rsy va'k dk vkdyu fu;j bUQzkjsM fj¶yDVsl

a LisDVªksLdksih ¼,uvkbZvkj,l½
dqekj o lkFkh] 2003 }kjk fd;k tk,xkA 'kq"d cht esa rsy va'k dk irk dkVs x,
chtksa ls ,uvkbZvkj vkSj ,u,evkj ¼cht dks u rksM+us okyh rduhd½ fof/k }kjk
fd;k tkrk gSA ,uvkbZvkj,l fo'ys"k.k fM¶;wl fj¶ysDVsl
a LiSDVªksLdkWih ds fl)kar
ij vk/kkfjr gSA bl rduhd esa 0-8&2-5  m ijkl dh ÅtkZ dk ekiu fd;k tkrk
gS tks uewuksa ls folfjr gksdj ijkofrZr gksrh gSA ,d lesfdr o`Ùkkdkj fMVsDVj uewus
ls ijkofrZr gksus okyh yxHkx [Link] ÅtkZ dks ukirk gS] uewus ls rjax yackbZ dh
Ük`a[kyk ij ijkofrZr ;g [Link] mlh rjax yackbZ ij ekud lanHkZ lrg ds ijkorZu
ds uki ij vk/kkfjr gksrk gSA bl fof/k esa cgqr FkksM+h ÅtkZ 'kksf"kr gksrh gS vkSj
ijkorZu dk dks.k vkiru dks.k ds cjkcj gksrk gSA vkikrh fdj.k vYi nwjh ij uewus
dh lrg ij izos'k djrh gS vkSj uewus esa [Link] ds [Link] rd dEiu ÅtkZ dks
gLrkarfjr djrh gSA ;g ÅtkZ rc gLrkarfjr gksrh gS tc vkikrh [Link] dh
vkorZrk jklk;fud ckW.M dh vkorZrk ¼ewyHkwr ;k vksojVksu½ ds cjkcj gksr h gSA
uewuksa dh og Ük`a[kyk ftlesa fu/kkZfjr ek=k esa lkanz.k gksrk gS] LdSu dh tkrh gS rkfd
fo'ysf"kr lkanz.k rFkk ,uvkbZvkj [Link] ds vo'kks"k.k ds chp ds lg&laca/k dk irk
yxk;k tk ldsA [Link]; lg&laca/k :ikarj.k ls ,d lehdj.k rS;kj fd;k tk
ldrk gS ftlls fo'ysf"kr inkFkZ dh lkanzrk Kkr dh tk ldrh gSA bl fof/k esa
fdlh uewus dh rS;kjh ds nkSjku vYi [Link] izos'k dh vko';drk gksrh gS vkSj
blls rjy ,oa Bksl nksuksa izdkj ds uewuksa dk fo'ys"k.k fd;k tk ldrk gSA

;g Kkr gS fd dqN oLrq,a fof'k"V rjax yackbZ ij izdk'k ÅtkZ dks vo'kksf"kr
djrh gSaA mnkgj.k ds fy, ueh yxHkx vojDr izdk'k ds 1-94  m cSaM dks
vo'kksf"kr djrh gS] izksVhu 2-18  m cSaM dks rFkk rsy 2-31  m ls 2-33  m rd
 15

cSaMksa dks vo'kksf"kr djrk gSA fdlh uewus dks fudVre vojDr izdk'k dh fof'k"V
rjax yackbZ ls [Link] djus ls fo'ysf"kr dh izfr'kr lkanzrk dk vuqeku yxkuk
laHko gS vkSj ,slk ml ijkofrZr ÅtkZ dks ukidj fd;k tk ldrk gS tks vo'kksf"kr
ÅtkZ ds foykser% lekuqikrh gksrh gSA

,d czkWM cSaM okyk VaxLVsu&gSykstu ySEi fudVre vojDr rjax yackb;ksa esa
izdk'k miyC/k djkrk gSA ,d ysl
a tks ySEi ds uhps fLFkr gksrk gS] izdk'k dks
lekukarj [Link] esa Qksdl djrk gSA ;g izdk'kiqat le;&le; ij pkSij Oghy }kjk
vojksf/kr gksrk gS ftlls fMVsDVj dks ,dkarfjd ladsr feyrs gSa vkSj bl izdkj iBuksa
dh fLFkjrk c<+ tkrh gSA pkSi fd;k x;k izdk'k ,uvkjvkbZ fQYVjksa ds ek/;e ls
xqtjrk gS tks fudVre vojDr izdk'k ds pqus gq, cSaMksa dks gh uewus dks [Link]
djus ds fy, xqtjus nsrs gSaA ,d NksVk lk fNnz ckgj ds lHkh izdk'k dks jksdrk gS
vkSj dsoy fQYVj fd;k gqvk o dkWyeusfVM izdk'k gh uewus ls gksdj xqtj ikrk gSA
dqN fudVre vojDr izdk'k uewus }kjk vo'kksf"kr gks tkrk gS vkSj 'ks"k ijkofrZr gks
tkrk gSA fMVsDVj ijkofrZr gksus okys folfjr izdk'k dh ÅtkZ dks uki ysrk gSA
fMVsDVj dk ladsr vkof/kZr gksrk gS vkSj vxys fo'ys"k.k ds fy, fMftVy Lo:i esa
ifjofrZr gks tkrk gSA

izR;sd fQYVj ds fy, ukih xbZ ijkorhZ ÅtkZ ,sls ;kaf=d ykWxfs jn~e esa
ifjofrZr gks tkrh gS ftldk mi;ksx ifj'kks/ku fu;rkaddksa ds :i esa fd;k tkrk gS
ftlls inkFkZ dh lkanzrk dk vuqeku yxk;k tk ldrk gSA ;g lehdj.k gS %
lkanzrk (%) = KA + K0 x Log (1/R0) + K1 x log (1/R1)
-----------------+ Kn + log (1/Rn)
;gka KA = ifj'kks/ku ds fy, fc;kl dk lek;kstu gS(
K0 = izFke fQYVj dh fLFkfr ds fy, [Link] gS
Log (1/R0) = ukis x, ijkorZu ¼vo'kks"k.k½ dk izFke ;kaf=d ykWx gS
 16

K1 = ikjLifjd ijkorZu ¼vo'kks"k.k½ ds f}rh; fQYVj dk ;kaf=d ykWxfjn~e gS

IX lanHkZ lkfgR;
1. dqekj] ,l-] ,-ds- flag] ,e- dqekj] ,l-ds-;kno] ts- ,l-pkSgku vkSj ih- vkj-
dqekj- 2003- LVsM a MkZbts'ku vkWQ fu;j bUÝkjsM fj¶ysDVsl a LisDVªksLdkWih
¼,uvkbZvkj,l½ QkWj fMVjfeus’ku vkWQ lhM vkW;y ,aM çksVhu daVsUV~l bu
jsilhM & eLVMZ- ts-QwM lkabl VSDuks- 40% 306&309-
2. dqekj] ,l-] ,l-ds- ;kno] ts-,l-pkSgku] ,-ds- flag] ,u-,-[kku vkSj ih- vkj-
dqekj- 2003- VksVy XywdkslkbuksysV ,LVhes’ku ck; dkEIySDl QkWe’sZ ku fcVohu
XywdkslkbuksysV~l ,aM VsVªkDyksjksisykMsV ¼ II½ ;wftax
+ ,ykbtk+ jhMj- ts- QwM
lkbal VSDuks- 41%63&65-
3. iSSDokV] lh- rFkk gkmVQSuh] ,- 1987- [Link] eSFkM~l QkWj n ,ukfyfll vkWQ
vkW;Yl] QSV~l ,aM MsfjosfVOl- CySdoSy lkbafVfQd ifCy’klZ] vkWDlQksMZ- eq-i`-
73&77-
X. dk;Z cy dk fooj.k
;s ijh{k.k fn'kkfuns'Z k jk"Vªh; rksfj;k&ljlksa vuqla/kku dsU nz] Hkjriqj( uksMy vf/kdkjh]
Mh;w,l ijh{k.k dsUnz rFkk ikS/kk fdLe vkSj d`"kd vf/kdkj laj{k.k izkf/kdj.k }kjk xfBr
dk;Z cy ¼2@2006½ ds ijke'kZ ls jk"Vªh; dksj lfefr }kjk fodflr fd;k x;k gSA

dk;Z cy ¼2@2006½ ds lnL; %

MkW- okbZ ,l usj dj ¼v/;{k½
MkW- ,l ,l ukjk;.ku
MkW- Mh ,e gsxM+s
MkW- ih ,l ikBd
MkW- ,p ,l lsu
MkW- vkj ds pkS/kjh
MkW- ,l ,l ckaxk
MkW- , ds flag
MkW- ih ,l HkVukxj
 17

uksM y vf/kdkjh
1- MkW- ds-,p-flag] ofj"B oSKkfud] jk"Vªh; ljlksa vuqla/kku dsUnz] Hkjriqj
2- MkW- ,-ds-feJk] ofj"B oSKkfud] jk"Vªh; ljlksa vuqla/kku dsUnz] Hkjriqj

X. Mh;w,l ijh{k.k dsUnz dk uke

uksMy Mh;w,l ijh{k.k dsUnz vU; Mh;w,l ijh{k.k dsUnz

czSfldk tqafl;k czSfldk dSfjukVk
rksfj;k&ljlksa vuqla/kku funs'kky;] pUnz 'ks[kj vktkn pkS/kjh pj.k flag
lsokj] Hkjriqj&321303 jktLFkku d`f"k ,oa izkS|ksfxdh gfj;[Link] d`f"k
fo'ofo|ky;] dkuiqj fo'ofo|ky;] fglkj
pkS/kjh pj.k flag
gfj;[Link] d`f"k
fo'ofo|ky;] fglkj
 18

Indian Mustard (Brassica juncea L. Czern & Coss) and Karan Rai
(Brassica carinata A Braun)

I. Subject

These test guidelines shall apply to all varieties, hybrids, transgenics and parental lines of
Indian mustard (Brassica juncea L. Czern & Coss) and Karan rai (Brassica carinata A
Braun).
II. Seed material required
1. The Protection of Plant Varieties and Farmers' Rights Authority (PPV & FRA) shall
decide when, where and in what quantity and quality of the seed material are required for
testing a variety denomination applied for registration under the Protection of Plant
Varieties and Farmers' Rights (PPV & FR) Act, 2001. Applicants submitting such seed
material from a country other than India shall make sure that all customs and quarantine
requirements stipulated under relevant national legislations and regulations are complied
with. The minimum quantity of the seed to be provided by the applicant shall be 500
gram in the case of the candidate variety or hybrid and 250 gram for each of the parental
line of the hybrid. Each of these seed lots shall be packed, sealed and properly labeled
with details in ten equal weighing packets and submitted in one lot. Parental lines should
be packed in one packet.
2. The seed submitted shall have at least 85 % germination, 98 % physical purity, highest
genetic purity, uniformity, sanitary and phytosanitary standards. In addition, the moisture
content of the seed shall not exceed 8 % to meet the safe storage requirement. The
applicant shall also submit along with the seed, a certified data on germination test made
not more than one month prior to the date of submission.
3. The seed material submitted shall not have been subjected to any chemical or biophysical
treatment.
III. Conduct of tests
1. The minimum duration of DUS tests shall normally be at least two independent similar
growing seasons.
2. The test shall normally be conducted at least at two test locations. If any essential
characteristics of the candidate variety are not expressed for visual observation at these
 19

locations, the variety shall be considered for further examination at another appropriate
test site or under special test protocol on expressed request of the applicant.
3. The field test shall be carried out under conditions favouring normal growth and
expression of all test characteristics. The size of the plots shall be such that plants or
parts of plants could be removed for measurement and observation without prejudicing
the observations on the standing plants until the end of the growing period. Each test
shall include about 700 plants, in the plot size and planting space specified below
across three replications. Separate plots for observation and for measurement can only
be used if they have been subjected to similar environmental conditions. All the
replications shall be sharing similar environmental conditions of the test location.
4. Test plot design
Number of rows : 6
Row length : 6m
Row to row distance : 45 cm
Plant to plant distance : 15 cm
Expected total number of plants : 720
Number of replications : 3
5. Observations should not be recorded on plants in border rows.
6. Additional test protocol for special purpose shall be established by the PPV & FR
Authority.

IV. Methods and observations

1. The characteristics described in the Table of characteristics (see section VII) shall be
used for the testing of varieties, inbred lines and hybrids for their DUS.
2. For the assessment of Distinctiveness and Stability, observations shall be made on 60
plants or parts of 60 plants, which shall be equally divided among 3 replications (20
plants per replication).
3. For the assessment of Uniformity of characteristics on the plot as a whole (visual
assessment by a single observation of a group of plants or parts of plants), of parental
lines a population standard of 2 % with an acceptance probability of at least 95 % and for
varieties and hybrids, a population standard of 5 % with an acceptance probability of at
 20

least 95 % shall be applied. In the case of a sample size of 700 plants the number of off-
types should not exceed 10 in parental lines and 25 in varieties and hybrids.
4. For the assessment of all colour characteristics, the latest Royal Horticultural Society
(RHS) colour chart shall be used.
5. Unless otherwise indicated, all observations on the leaf shall be made on the fully
developed leaves in between bud formation and flower initiation.

V. Grouping of varieties
1. The candidate varieties for DUS testing shall be divided into groups to facilitate the
assessment of Distinctiveness. Characteristics, which are known from experience not to
vary, or to vary only slightly within a variety and which in their various states are fairly
evenly distributed across all varieties in the collection are suitable for grouping purpose.
2. The following characteristics shall be used for grouping Indian mustard and Karan rai
varieties:

i) Leaf : Number of lobes (Characteristic. 4)

ii) Flower : Time of flowering (Characteristic. 8)
iii) Plant : Main shoot length (Characteristic. 12)
iv) Siliqua : Number of seeds per siliqua (Characteristic. 20)

VI. Characteristics and symbols

1. To assess Distinctiveness, Uniformity and Stability, the characteristics and their states as
given in the Table of characteristics (Section VII) shall be used.
2. Note (1 to 9), shall be used to describe the state of each character for the purpose of
digital data processing.
3. Legend:
(*) Characteristics that shall be observed during every growing season on all varieties and
shall always be included in the description of the variety, except when the state of expression
of any of these characters is rendered impossible by a preceding phenological characteristic
or by the environmental conditions of the testing region. Under such exceptional situation,
adequate explanation shall be provided.
 21

(+) See explanation on the Table of characteristics in section VIII. I t is to be noted that for
certain characteristics, the plant parts on which observations to be taken are given in the
explanation of figure(s) for clarity and not for colour variation.
4. A decimal code number in the seventh column of table of characteristics indicates the
optimum stage for the observation of each characteristic during the growth and
development of plant. The relevant growth stages corresponding to these decimal code
numbers are described below:
Decimal code for the growth stages:
Code Growth stage
00 Dry seed
50 Bud formation
60 Flower initiation
62 Few buds are open on terminal raceme
79 All seeds of siliquae on terminal raceme are dark
85 Maturation
90 Seeds in upper siliquae show brown areas
100 After harvest

5. Type of assessment of characteristics indicated in column eighth of table of

characteristics is as follows:
MG: Measurement by a single observation of a group of plants or parts of plants
MS: Measurement of a number of individual plants or parts of plants
VG: Visual assessment by a single observation of a group of plants or part of plants
VS: Visual assessment by observations of individual plants or parts of plants
 22

VII. Table of Characteristics

S. Characteristics States Notes Example varieties Stage of Type of

No observation assessment
. B. juncea B. carinata (Code No.)

1 2 3 4 5 6 7 8
1. Leaf: Hairiness Absent 1 Basanti, RH 781 Kiran, PC 5 50-60 VS
(+) Sparse 3 Varuna, Pusa Bold -
Dense 5 CS 52, Geeta -
2. Leaf: Colour Light green 1 NDRE 4 - 50-60 VG
(*) Medium 2 Varuna, BIO 902 -
green
Dark green 3 GM 1 Kiran, PC 5
3. Leaf: Lobes Absent 1 - - 50-60 VS
(*) Present 9 Varuna Kiran, PC 5
(+)
4. Leaf: Number of Low ( < 5) 3 - - 50-60 MS
(*) lobes Medium 5 Kranti PC 5
(6 – <8)
High (> 8) 7 CS 52, RH 819 Kiran
5. Leaf: Dentation Entire 1 - Kiran, PC 5 50-60 VS
(*) of margin Dentate 2 Varuna, BIO 902 -
(+)
Serrate 3 NDRE 4
6. Leaf: Length B. juncea
(cm) Short ( < 25 ) 3 NDRE 4 50-60 MS

Medium 5 Varuna
(26 – <30 )
Long 7 RH 781, PCR 7
(>30 )
B. carinata
Short 3 - 50-60 MS
( < 30 )
Medium 5 PC 5
(31-<35)
Long (> 35 ) 7 Kiran
7. Leaf: Width Narrow (< 3 NDRE 4 - 50-60 MS
(cm) 10)
Medium 5 Varuna, GM 1 PC 5
(10 –12)
Broad 7 RH 781, PCR 7 Kiran
(> 12 )
 23

8. Flower: Time of B. juncea

(*) flowering Early 3 NDRE 4 60-62 MG
(50 % of the ( <40 days)
plant with at least Medium 5 BIO 902, GM 1
one open flower) (41 - <50
days)
Late 7 RH 8113
(> 50 days)
B. carinata
Early 3 - 60-62 MG
(< 50 days)
Medium 5 PC 5
(51 – <60
days)
Late 7 Kiran
(> 60 days)
9. Flower: Colour White 1 - - 60-62 VG
(*) of petals Light yellow 2 Pusa Mahak -
Yellow 3 Varuna, BIO 902 Kiran, PC 5
Orange 4 - -
10. Flower: Length Short(<1.2 ) 3 NDRE 4 - 60-62 MS
of petals Medium 5 Pusa Bold, Rohini Kiran
(cm) (1.2-1.5 )
Long (>1.5 ) 7 - PC 5
11. Flower: Width of Narrow 3 - - 60-62 MS
petals(cm) (<0.6)
Medium 5 Basanti, BIO 902 -
(0.6-0.7 )
Broad (>0.7 ) 7 RL 1359 Kiran, PC 5

12. Plant: Main shoot Short 3 - Kiran 79 MS

(*) length ( < 40 )
(cm) Medium 5 RCC 4 JTC 1
(41- <50 )
Long 7 GM 1 -
(51 - <60 )
Very long 9 Rohini, Geeta -
(>60 )
13. Plant : Height Short (< 130 3 NDRE 4 - 79 MS
(*) (cm) )
Medium 5 S. Asech -
(131- <150)
Tall 7 Varuna, Pusa Bold Kiran
(151 - <170 )
Very tall 9 Basanti, RH 819 PC 5
(> 170 )
14. Siliqua: Length Short 3 Kanti Kiran 85 MS
(*) (cm) ( < 4.5 )
 24

(+) Medium 5 RH 30 PC 5
(4.5-5.5 )
Long 7 Pusa Bold -
(> 5.5 )
15. Siliqua: Length Short 3 Geeta PC 5 85 MS
of beak (<0.8 )
(cm) Medium 5 PBR 97, PCR 7 -
(0.8 – <1.2 )
Long 7 Pusa Bahar -
(> 1.2 )
16. Siliqua: Number Very few 3 NDRE 4 Kiran, PC 5 85 MS
(*) on main shoot (<40)
Few (41 - 5 Varuna JTC 1
<50)
Medium 7 Rohini -
(51 - <60)
Many (> 60) 9 Geeta -
17. Siliqua: Density Low (<0.7) 3 NDRE 4 PC 5 85 MS
(+) on main shoot Medium 5 Varuna -
(0.7 - 0.8)
High (>0.8) 7 - Kiran
18. Siliqua: Angle Appressed 1 Sanjucta Asech Pusa 85 VG
(*) with main shoot Swarnim
(+) Semi 2 Rohini, Geeta PC 5
appressed
Open 3 Varuna Kiran
19. Siliqua : Texture Smooth 3 - - 85 VS
(*) Undulated 5 Varuna -
(+)
Constricted 7 Basanti Kiran, PC 5
20. Siliqua: Number Very few 3 - - 85 MS
(*) of seeds per ( <12)
siliqua Few (13-<16) 5 GM 1, Varuna PC 5
Medium (17- 7 CS 52, Sej-2 -
<20)
Many (> 20) 9 Geeta -
21. Maturity period B. juncea
(*) Early 3 NDRE 4 90 MG
(+) ( <110 days)
Medium 5 SEJ 2
(111 -<130
days)
Late 7 Varuna, Rohini
(131-<150
days)
Very late 9 -
(> 150 days)
 25

B. carinata
Early (<120 3 - 90 MG
days)
Medium (120 5 PC 5
-<140 days)
Late (140- 7 Kiran
<160 days)
Very late (> 9 -
160 days)
22. Seed: Seed Yellow 1 Basanti Kiran 100 VG
(*) colour Reddish 2 CS 52 PC 5
brown
Brown 3 RCC 4 -
Dark brown 4 BIO 902 -
Black 5 - -
23. Seed: Size B. juncea
(*) (Weight of 1000 Small (<5.0 3 Kranti 100 MG
seeds) g)
Medium 5 Rohini
(5.0-6.0 g)
Bold (>6.0 g) 7 Pusa Bold
B. carinata
Small (<4.0 3 - 100 MG
g)
Medium 5 Kiran, PC 5
(4.0-6.0 g)
Bold (>6.0 g) 7 -
24. Low (<38) 3 - PC 5 100 MG
(*) Seed: Oil content Medium 5 CS 52, Varuna Kiran
(+) (%) (38 – <42 )
High (42- 46) 7 Rohini -
Very high 9 - -
(>46)
 26

VIII. Explanation on the Table of characteristics

Characteristic 1. Leaf: Hairiness
Leaf hairiness should be observed on lower side of the leaf.

1 3 5
Absent Sparse Dense

Characteristics 3. Leaf: Lobes

Absence or presence of lobes should be observed on the fully developed leaf between bud
formation to flower initiation stage. Parts of the leaf blade are considered as lobes if their length
is at least equivalent to the width of the leaf petiole at their point of attachment and if the upper
notch of the blade has at least half the length of the lobe itself. Secondary lobe(s) are not
counted.

Characteristic 5. Leaf: Dentation of margin

Dentation of leaf should be observed on upper one third part of the leaf blade.

1 2 3
Entire Dentate Serrate
 27

Characteristic 14. Siliqua: Length

Siliqua length between pedicel and beak. It should be measured at lower one third portion of
main shoot.

Characteristic 17. Siliqua: Density on main shoot

It should be computed as a ratio between number of Siliquae born on main shoot and main shoot
length.

Characteristic 18. Siliqua: Angle with main shoot

Siliqua angle should be observed between main shoot and pedicel at lower one-third portion of
main shoot.

1 2 3
Appressed Semi-appressed Open

Characteristic 19. Siliqua: Texture

3 5 7
Smooth Undulated Constricted
 28

Characteristic 21. Maturity period

Maturity duration should be recorded as days from date of sowing to when 75 % of siliquae turn
yellowish.

Characteristic 24. Seed: Oil content

Oil content of the seed shall be estimated by the Near Infrared Reflectance Spectroscopy
(NIRS) Kumar et. al. 2003. Oil content of dry seed is determined using NIR & NMR (Non
destructive techniques) from the harvested seeds. Near infrared reflectance spectroscopy (NIRS)
analysis is a technique based on the principle of diffuse reflectance spectroscopy. This technique
utilizes measurements of energy in the 0.8-2.5 µm range which is diffusely reflected from the
samples. An integrating sphere detector measures almost all energy reflected from the sample.
The reflected radiation from the sample at a series of wavelength is followed by mea surements
of reflectance of a standard reference surface at the same wavelengths. The reflectance from the
sample is reported relative to the standard reflector. In this method, little radiation is absorbed
and the angle of reflectance is equal to the angle of incidence. The incident radiation penetrates
the surface of sample a small distant and can transfer vibrational energy to the bonds of the
molecules in the sample. The energy is transferred when the frequency of the incident radiation
is the same as the frequency (Fundamental or overtone) of the chemical bond. A series of sample
that contain known amounts of a given concentration are scanned to find correlation between the
analyte concentration and the absorption of NIR radiation. With a mathematical correlation
transform, an equation can be developed to determine the concentration of analyte. The method
requires little, if any sample preparation and can handle both the liquid and solid samples.

It is known that certain constituents absorb light energy at specific wavelengths. For
instance, moisture absorbs the maximum at 1.94 µm band of near infrared light, protein adsorbs
at 2.18 µm band and oil between 2.31 µm and 2.33 µm bands. By irradiating a sample with
specific wavelength of near infrared light, it is possible to predict the per cent concentration of
analyte by measuring the energy reflected which is inversely proportional to the energy
absorbed.
A broad band tungsten- halogen lamp provides light rich in near infrared wavelengths. A
lens, located below the lamp focuses the light into parallel rays. The light beam is periodically
interrupted by the chopper wheel to provide an alternating signal to the detector and thus
 29

enhance the stability of the readings. The chopped light is passed through NIR filters, which
allow only the selected bands of near infrared light to pass through them irradiate the sample. An
aperture block all outside light and allow only the filtered, columnated light to pass through the
sample. Some of the near infrared light is absorbed by the sample and the rest is reflected. The
detector measures the energy of the diffused light being reflected. The detector signal is
amplified and converted in to digital form for further processing.
The measured reflectance energy, for each filter, is converted to a machine logarithm that
is used along with the calibration constants to predict concentration of the constituent. The
equation is
Concentration (%) = K A + K0 x Log (1/R0 ) + K 1 x log (1/R1 )
-----------------+ Kn + log (1/Rn )
Where, K A is the bias adjustment for the calibration;
K 0 is the coefficient for the first filter position ;
Log (1/R0 ) is the first machine log of the measured reflectance (absorption).
K 1 is the second filter’s machine logarithm of the reciprocal reflectance (absorption).

IX. References:

Kumar, S., A. K. Singh, M. Kumar, S.K. Yadav, J. S. Chauhan and P.R. Kumar. 2003.
Standardization of Near Infrared Reflectance Spectroscopy (NIRS) for determination of seed oil
and protein contents in Rapeseed – Mustard. J. Food Sci. Techno. 40: 306-309.

Kumar, S., S.K. Yadav, J. S. Chauhan, A. K. Singh, N. A. Khan and P.R. Kumar. 2004.
Total glucosinolate estimation by complex formation between glucosinolates and
Tetrachloropalladate (II) using ELISA Reader. J. Food Sci. Technol. 41 : 63-65.

Paquot, C. and Hautfenne, A. 1987. Standards methods for the analysis of oils, fats and
derivatives. Blackwell Scietific Publishers, Oxford. Pp. 73-77.
 30

X. Working Group details:

These test guidelines developed by the National Core Committee in consultation with the,
Directorate of Rapeseed-Mustard Research, Bharatpur, the Nodal Officer, DUS test centre and
Task Force (2/2006) constituted by the PPV&FR Authority.

The Members of the Task Force (2/2006)

Dr. Y. S. Nerkar Chairman
Dr. S. S. Narayanan
Dr. D. M. Hegde
Dr. P. S. Pathak
Dr. H. [Link]
Dr. R. [Link]
Dr. S. S. Banga
Dr. A. K. Singh
Dr. P. S. Bhatnagar

Nodal Officer:

1. Dr. K.H Singh, Senior Scientist, DRMR, Bharatpur(Rajasthan)

2. Dr. A.K Misra, Senior Scientist, DRMR, Bharatpur(Rajasthan)

XI. Name of DUS Test Centre:

Nodal DUS Test Centre Ot Othe r DUS Test Centres
Brassica juncea Brassica carinata
Directorate of Rapeseed-Mustard CSAUAT, Kanpur CCSHAU, Hisar
Research, Sewar, Bharatpur-321303 CCSHAU, Hisar