DH36 Auto Hematology Analyzer Service Manualce Thank you for purchasing the Auto Hematology Analyzer manufactured by Dymind Biotech. Preface Before performing maintenance on the device, please read this service manual carefully to gain an ‘understanding of how to properly maintain it. After reading, keep the manual in a convenient location for furure reference and easy access, Product name: Auto Hematology Analyzer Model: DH36 Contact Info for After-Sales Services Manufacturer: Shenzhen Dymind Biotechnology Co., Ltd, Address: 4/F, Block A, Sibiono Gene Treatment Area, No.9 Keji Zhongyi Rd., Nanshan District, Shenzhen 518057 [Link] uropean Representative: $ Bankside, Hanborough Business Park, Witney 0x29 SLT UK Zip Code: 318057 24/7 Service Hotline: 400-998-7276 Tel.: +86 755 26008015 Fax: +86 755 26746162 Email: dymindédymind. com. cn Website: http: //mww. dymind. [Link] Intellectual Property Rights This document contains proprietary information of Shenzhen Dymind Biotechnology Co., Ltd. (hereinafter referred to as Dymind Biotech). The corresponding product is the intellectual property of Dymind Biotech. ‘No part of this service manual may be reproduced, copied, modified or translated in any form or by any ‘means without prior written consent of Dymind Biotech. Copyright © SHENZHEN DYMIND BIOTECHNOLOGY CO., LTD. iiContents Declaration Dyinind Biotech reserves the right of final interpretation of this service manual. ‘Dymind Biotech shall be responsible for the safe of the following conditions are met: secuuity, and performance of the product only when all © The assembly, re-commissioning, extension, modification, and repair of the product are performed by authorized personnel of Dymind Biotech. © The installation of the relevant electrical devices complies with applicable national standards. © The product is operated in accordance with this service manual. Maintenance Services Scope of free services: Al products complying with Dymind Biotech’s Product Warranty specifications may qualify for free secviees. Scope of fee-based services: © Free services are available to all products exceeding Dymind Biotech's Product Warranty specifications. © Products within the warranty period require maintenance under the following circumstances: > Artificial damage > Improper use > When grid voltage exceeds the specified operational range > Unavoidable natural disasters > When parts and supplies are replaced with no prior consent from Dymind Biotech, or machine ‘maintenance is performed by personnel with no prior authorization fom Dymind Biotech A WARNING Any failure by hospitals or organizations responsible for using this instrument to implement a competent repairimaintenance plan is likely to result in abnormal instrument failure or even a health hazard. © Make sure the analyzer is only operated under the conditions of use as specified in the operator's ‘manual. If operated outside of the specified conditions of use, the analyzer may not work properly, ‘which could lead to unreliable measurement results and damaged instrument components or even bodily injury. This analyzer must be operated by trained medical testing professionals, doctors, nurses, or laboratory technicians. Copyright © SHENZHEN DYMIND BIOTECHNOLOGY CO., LTD. wvContents Contents 1 1.1 Who Should Read This Manual 1 1.2 Symbols and Legents... wal 1.3 Safety Instructions 3 2 Tushruiitint Conifigara tO winiininmisisninrenisiiinnmenintiiminiiimniitisisinmnaiiiinciiin’S 321 Aipchatical Component iiisnsnssucsinnniniinirininisisininnninsssstenniisinucoonansunniatl 2.2 Hydraulics System, 8 2.2.1 Schematic Diagram of the Hydraulic System. sl 2 Hydraulics Diagram for the Overall Unit, wed 2.23 WBC/HGB Measurement Channel 10 2.24 RBCPLT Measurement Module u 2.5 Sampling and Blood-dispensing Module... 2.6 Power Supply and Waste Discharge Module 12 2.2.7 Status Monitoring Module : 12 2.2.8 Hydraulic Components 2.2.9 Main Measurement Modes "7 2.2.10 Hydraulies Maintenance 2 2.2.11 Troubleshooting Common Hydraulics Problems 2.3 Hardware System 2.3.1 Main Control Panel. 35 2.3.2 Other Panels. so 3 Repairs. 3.1 Overview 53 3.2 Preparatory Work before Repairs... 3.2.1 Open the Left Side Panel 33 3.2.2 Open the Right Side Doot....[Link] 3.2.3 Disassembling the Front Panel Cover... 3.3 Display Screen Assembly Replacement 3.4 Sampling Assembly Replacement. 59 3.4.1 Replacing the Sample probe 59 3.4.2 Replacing the Optocoupler 3.4.3 Replacing the Sampling Assembly in X- or ¥-direction 6 3.5 Power Assembly Replacement ae Copyright © SHENZHEN DYMIND BIOTECHNOLOGY CO., LTD. vContents 3.6 Replacing the Hydraulics Components incl. Valves, Pumps, and Pressure Chambers. 3.6.1 Replacing the valve assembly, so 3.6.2 Replacing the Liquid Pump Assembly, 65 3.6.3 Replacing the Negative-pressure Chamber Assembly a 3.7 Sheath Flow Syringe Assembly Replacement 3.7.4 Replacing the syringe... 3.7.2 Replacing the Motor se 3.8 WBC and RBC Bath Assemblies Replacement 3.8.1 Dismantling and replacing the WBC bath assembly... 3.8.2 Dismantling and replacing the RBC bath assembly... 3.9 Main Control Panel Replacement. 3.10 Replacement of Reagent Testing Panel 8 3.11 Temperature Sensor Replacement 80 3.11.1 Disassembling and Replacing the Temperature Sensor. 80 4 Software Upgrade snmmmmmnmnmenmemnmnmmnemmmmnenennnnennmnmnmmnmnnnmnmennnners SE 4.1 Preparation 82 4.2 Uperading Steps 4.3 Touch Screen Calibration 5 Comprehensive Device Tuning .. 5.1 Position Adjustment... . 86 5. HGB Voltage Gain Setting . 87 5.3 Gain Calibration 88 3.4 Calibration of CalibratOr8 ..unneonne 88 5.4.1 Calibration in Whole-blood Mode 88 5.4.2 Calibration in Preditute Mode. 89 6 Alarms and Solutions, 7 Maintenance Inventory, nninnninnnnnnnsiinnnininnnnnnnnnnninnninnnnnnsies 9S Copyright © SHENZHEN DYMIND BIOTECHNOLOGY CO., LTD. vi1 Manual Overview 1 Manual Overview This chapter explains the procedures manval carefully to guarantee proper device maintenance and operator safety. ‘Analyzer. Please read this This manual is complemented by the operator’s manual; there is no overlap between the two. NOTE Be sure to operate the device in strict accordance with the instructions in the maintenance and operator's manuals, 1.1 Who Should Read This Manual This service manual should be used by professionals who possess: © A comprehens © A comprehensive knowledge of reagents re knowledge of electrical circuits and hydraulics A comprehensive knowledge of quality control A comprehensive knowledge of troubleshooting An understanding of basic mechanical tools and any relevant terms Skills for using digital voltmeters and oscilloscopes ‘The ability to analyze electrical-circuit graphs and hydraulics diagrams, and to understand the related terminolo; . . © ‘The ability to skillfully operate this analyzer . . ° 1.2 Symbols and Legends Symbols used in the manual: Symbol It means The operator should follow the instruction below each symbol to WARNING | 20H personal injury ‘The operator should follow the instructions below each symbol to CAUTION | P*vent instrument failure, damage, or disrupted test results. Copyright © SHENZHEN DYMIND BIOTECHNOLOGY CO.,LTD, 11 Manual Overview Symbol It means NOTE The operator should follow the instructions below each symbol and pay special attention to any pertinent information when following the procedures ® ‘The operator should follow the instructions below each symbol to prevent any danger of infection The following symbols can be found on the analyzer, reagents, QC materials, or calibrator: Symbol Tt means A Refer to the operator's manual shipped with the product. A Biohazard Protective grounding ~ Alternating current (AC) IVD For in vitro diagnosis only R ‘Type B applied part LOT Lot No. Exp. date Serial No. Date of manufacture “Manufacturer Storage temperature hal s| 2: (g]/© Consult the operator's manual Copyright © SHENZHEN DYMIND BIOTECHNOLOGY CO.,LTD,1 Manual Overview 1.3 Safety Instructions Device maintenance done in accordance with the following instructions guarantees the safety of both patients and operators, A WARNING Any failure by hospitals or organizations responsible for using this instrument to implement competent repairimaintenance plan is likely to result in abnormal instrument failure or even a health azar. © Toprevent risk of explosion, do not use combustible gases (e.g. anesthetics) of liquids (e.g, ethanol) near this product © The machine should be powered off while troubleshooting. Any maintenance operation while the ‘power is on can lead to electric shock or damage to its electrical components. © Please connect the device to the socket using a separate fuse and surge protection switch If the device shares a fuse and surge protection switch with other equipment, e.g. life support equipment, any ‘malfunction may cause an electric surge to occur when the instrument is powered on, which can trip the circuit breaker © Maintenance personnel must keep their clothes, har, and hands away from moving parts such as the sample probe, jaws, and puncture needle to avoid being stabbed or pinched during maintenance. © Parts with special warnings may be subject to mechanical movements and thus lead to injuries from pinching or stabbing during normal operation or disassembly and repair. © Operators are obligated to comply with local and national regulations with regard to the disposal and emission of expired reagents, waste, waste samples, consumables, and so on. © The reagents are irvitating to eyes, skin and mucosa. When handling reagents and their related items in the laboratory, the operator should comply with laboratory safety regulations and wear personal. protective equipment (such as a protective lab suit, gloves, etc.) © Ifthe reagent accidentally comes in contact with your skin, wash it off immediately with plenty of water and see a doctor if [Link] the same if you accidentally get any of the reagent in your eyes A CAUTION © Improper maintenance may damage the analyzer. Maintenance personnel should maintain the device in accordance with the instructions contained in the service manual, and inspect the device properly after each maintenance. © Ifyou encounter a problem not specified in the service manual, please contact Dymind's aftersales service department. A professional will be assigned to offer you maintenance advice © When repairing the electronic components of the device, please take off any metal accessories you ‘might be wearing to prevent personal injury or damage to the device. © Electrostatic discharge may cause damage to the device's electronic components If the repair process can result in any electrostatic damage, please wear an antistatic wrist strap or maintain the device on an antistatic workstation. Copyright © SHENZHEN DYMIND BIOTECHNOLOGY CO.,LTD, 31 Manual Overview This analyzer must be operated by trained medical testing professionals, doctors, nurses, or laboratory technicians. & © Samples, controls, calibrators, and waste may pose a potential risk of bio-contamination, When handling reagents and related items in the laboratory, the operator should comply with laboratory safety regulations and wear personal protective equipment (such as a protective lab suit, gloves, etc.) © Both the parts and surface of the analyzer are potentially infectious, so please take safety precautions during operation and maintenance © The pointed sample probe, any blood stains left on it, QC materials, and calibrators may be potentially bio-infectious, so please avoid contact with the sample probe, Copyright © SHENZHEN DYMIND BIOTECHNOLOGY CO.,LTD, 42 Instrument Configuration Instrument Configuration 2.1 Mechanical Components ‘The DH36 3-part differential hematology analyzer consists of the main unit and its accessories Figure 2-1 Front view of the analyzer 5 3 o z 4 a 1 LJ T 1 - Sample aspiration touch panel 2- Sample probe 3 - Status indicator light 4 Thermal printer 5 - Display sereen Copyright © SHENZHEN DYMIND BIOTECHNOLOGY CO.,LTD, 52 Instrument Configuration jure 2-2 Analyzer Profile HTH w Ww J LAN network interface 2- USB interface 3 - Cam lock of the right side door 4- Sliding door lock of the small left side door Figure 2-3 Back view of the analyzer & 6 3 ie qe T 67 82 1 - Power switeh 2.- Power supply input connector 3 - Cooling fan 4-- Waste outlet connector 5 - Diluent inlet connector 6- BNC socket for the diluent sensor 7-BNC socket for the waste sensor 8 - Grounding pole Copyright © SHENZHEN DYMIND BIOTECHNOLOGY CO.,LTD,2 Instrument Configuration jure 2-4 Front view of the analyzer (panel cover, rear shell removed) Sampling assembly 2- Main control panel Figure 2-5 Right-side view of the analyzer (right-side door removed) oO EH er Oy * eRe 3 1 - Power assembly 2.- Sampling assembly 3 - Counting bath assembly 4 - Electromagnetic valve assembly 5 - Negative-pressure chamber 6 - Liquid pump assembly assembly Copyright © SHENZHEN DYMIND BIOTECHNOLOGY CO.,LTD,2 Instrument Configuration Figure 2-6 Left-side view of the analyzer (Left-side door removed) Power assembly 2 Reagent testing panel 3 - Flectromagnetic valve assembly 4- Sample syringe 2.2 Hydraulics System The hydraulics system consists of the WBC/HGB measurement module, the RBC/PLT measurement module, the sampling and blood dispensing module, te power supply and waste discharge module, and the status monitoring module. Detailed description: © WBCHGB measurement module Consists of the dosing syringe, WBC/HGB counting bath module, tubing, valve, HGB emission light, and HGB receiving tube. © RBC/PLT measurement module Consists of the dosing syringe, RBC/PLT counting bath assembly, tubing, and valve © Sampling and blood dispensing module: Consists of the sample probe, sample syringe, swabs, and sampling assembly. © Power supply and waste discharge module: Consists of the vacuum chamber, liquid pump, valve, and tubing, © Status monitoring module: Consists of the optocoupler and sensor. Copyright © SHENZHEN DYMIND BIOTECHNOLOGY CO.,LTD, 82 Instrument Configuration 2.2.1 Schematic Diagram of the Hydraulic System Figure 2-7 Schematic diagram of the hydraulic system WHole-blood CBC Mode Sampling ¥ [Dispensing] [DIL-E}—-+} Diluted sample Diluted sample LYE-1 WBCHGB measurement module 2.2.2 Hydraulics Diagram for the Overall U: Figure 2-8 Hydraulics diagram for the overall unit Hydraulles Diagram Copyright © SHENZHEN DYMIND BIOTECHNOLOGY CO.,LTD,2 Instrument Configuration 2.2.3 WBC/HGB Measurement Channel Channel Principles and a Description of Their Functions © Measurement principles: Impedance method and colorimetric method © Measurement parameters: WBC and HGB © Graph information: WBC histogram © Reagents for use > LYE-1: Lyses the RBC and combines with hemoglobin, > Diluent: This ditut and cleaning agent can provide an electrically conductive environment and process cells and so on in bulk. © Function description > The impedance method is used during WBC measurement: Afier the addition of LYE-I lyse, the red blood cells are lysed. Through the negative-pressure chamber (with @ negative pressure of -30Kpa), the sample is aspirated out of the WBC bath through the aperture. The WBC particles {generate electric pulses when traveling through the aperture, allowing the WBC cells to be counted according to the number of pulses emitted. > HGB is measured by colorimetry: Prior to the addition of the blood sample, the baseline voltage of the diluent is first measured. Then the blood sample and lyse are mixed well fora complete reaction so thatthe parameter voltage of the sample can be measured, HGB can then be calculated ‘based on the local voltage and parameter voltage according to Lambert-Beer’s Law. Measurement Process The measurement process for the WBC/HGB measurement module is as follows: 1. Dosing: The diluent syringe is first applied to add the diluent into the WBC bath, and then the sample probe is used to add the blood sample into the WBC bath, where they are mixed evenly. After aspirating the diluted sample for the first time, LYE-1 lyse is added into the WBC bath for incubation. 2. Mixing: Open Valve LV10 to generate air bubbles through the intermittent valve opening to mix the sample well. 3. Measurement: Open Valve LVO7 to aspirate the sample out of the WBC bath through the aperture by ‘means of the negative-pressure chamber (with a negative pressure of -30Kpa). The WBC particles generate electric pulses when traveling through the aperture, allowing the WBC calls to be counted according to the number of pulses emitted. 4. Cleaning: To clean, open LV02 and LV06, and add the diluent into the WBC bath using the dituent syringe. 5. Waste discharge: Waste is discharged by opening Valve LV09, LV10 and Pump Pl. Copyright © SHENZHEN DYMIND BIOTECHNOLOGY CO.,LTD, 102 Instrument Configuration 2.2.4 RBC/PLT Measurement Module Channel Principles and a Description of Their Functions © Measurement principles: Impedance method © Measurement parameters: RBC and PLT © Graph information: RBC histogram and PLT histogram. © Reagents for use Diluent: This diluting and cleaning agent can provide an electrically conductive environment and process cells and so on in bulk. © Function description RBC and PLT measurement makes use of the impedance method: After the addition of LYA-I lyse, the red blood cells are dissolved. Through the negative-pressure chamber (with a negative pressure of -30Kpa), the sample is aspirated out of the WBC bath through the aperture. The RBC and PLT particles generate electric pulses of various amplitudes when traveling through the aperture, allowing them to be counted according to the number of pulses emitted. Measurement Process Tie measurement process for the RBC/PLT measurement module is as follows 1. Dosing: The diluent syringe is first applied to add the diluent into the RBC/PLT bath as the base liquid, before using the sample probe to aspirate the sample diluted for the first time from the WBC bath into the RBC/PLT bath. 2. Mixing: Open Valve LV11 to generate air bubbles through the intermittent valve opening to mix the sample well ‘Measurement: Open Valve LVO7 to aspirate the sample out of the WBC bath through the aperture by ‘means of the negative-pressure chamber (with @ negative pressure of -30Kpa). The RBC and PLT particles generate electric pulses of various amplitudes when traveling through the aperture, allowing the RBC and PLT cells to be counted according to the number of pulses emitted. 4. Cleaning: To clean, open Valve LV02 and add the diluent into the RBC/PLT bath using the diluent syringe 5. Waste discharge: Waste is discharged by opening Valve LV09, LV11 and Pump PL 2.2.5 Sampling and Blood-dispensing Module This module consists of the sample probe, the sample injection tube, the sampling tube, the swab, the horizontal motor, and the vertical motor. This is how the sampling and blood-dispensing module works: Open LV04 and aspirate the sample from the testing tube using the sample syringe—Clean the outer wall of the sample probe ....unti it reaches the WBC bath—Put aside a fixed amount of blood in the WBC bath—-Clean the outer and inner wall of the sample probe—-Aspirate out of the WBC bath a fixed amount of the sample that has been diluted once—-Clean the outer wall of the sample probe and move the sample probe to the RBC bath—Add into the RBC bath the sample that has been diluted once—Clean the outer ‘wall of the sample probe to complete the entire process of sampling and blood dispensing. Copyright © SHENZHEN DYMIND BIOTECHNOLOGY CO.,LTD, "2 Instrument Configuration In summary, this module works by aspirating the sample and dispensing a fixed amount of the sample into the corresponding reaction baths, while cleaning the inner and outer walls of the sample probe to prevent cross-contamination, 2.2.6 Power Supply and Waste Discharge Module © Power supply module By opening LV09 and Pump PI to establish a negative pressure of -30Kppa, this module provides propulsion for the counting of the impedance channel. © Waste disposal module This module consists of the following five parts: > Discharge of waste resulting from swab cleansing LVO8 and Pump 09 are opened to discharge any waste resulting from using the swab to clean the sample probe. > Discharge of waste from the WBC bath ‘Valve LV09, LV10 and Pump Pi ate opened to discharge any waste from the RBC/PLT bath, > Discharge of waste from the RBC/PLT bath Valve LV09, LVI and Pump P1 are opened to discharge any waste from the RBC/PLT bath. > Discharge of waste fom the impedance counting bath After any waste from impedance counting (WBC counting and RBC/PLT counting) flows through the aperture, and after any waste that results from cleaning the rear section of the WBC and RBC/PLT baths flows into the negative-pressure chamber, Valve LV09 and Pump PI are opened for waste discharge, 2.2.7 Status Monitoring Module This module involves: © Monitoring of the diluent Detection is done by way ofa floating sensor; the float moves downwards the diluent level falls, and ‘an alarm is activated when it reaches empty. © Monitoring of LYE-1 An optocoupler is used to monitor the LYE-1 level: as it gets lower, the optocoupler’s status changes until an alarm is activated once there is no LYE-1 left. © Monitoring of waste overflow ‘Waste overflow is done by way of a floating sensor; as waste levels rise, the float moves upward until it eventually activates an alarm indicating that it is full. Copyright © SHENZHEN DYMIND BIOTECHNOLOGY CO.,LTD, 122 Instrument Configuration 2.2.8 Hydraulic Components List of Hydraulic Components No. | Component | Mlustration Material | Function Description. Remarks No. No. 1 | Sample probe 560101634 | Collects blood samples inthe | None testing tube and dispenses a certain portion of the sample to bbe placed in the corresponding counting bath 2 | swab 560100634 | Cleans the outer and inner walls. | None of the sample probe 3 | Two-way 241300104. | On-off switch for fuid control | None valve (L) 4 | Three-way 241300094 | Directional hydraulic valve | None valve (L) 3 | Two-way 241300024 | On-off'switeh for fuid control | None valve (S) 6 | Three-way 241300034 | Directional hydraulic valve | None valve (S) 7 | Liquid pump 240100014 | Discharges waste and creates a | None vacuum 8 | Sample 2001004 | Quantitatively divide the blood, | None syringe-ASP sand add with diluent and lyse Copyright © SHENZHEN DYMIND BIOTECHNOLOGY CO.,LTD. 132 Instrument Configuration No. ‘Component No. Mlustration Material No. Function Description Remarks: Isolation chamber 600100124 ‘This chamber insulates the liguid from the electric circuits to prevent any electrical interference. None 10 WBC/HGB, bath 200100074 1. This consists ofthe front bath and the rear bath, with an aperture in between: the sample flows from the front bath ‘through the aperture 10 generate electric pulses that facilitate WBC counting 2. The counting bath is accompanied by a HGB ‘emission light and a HBC signal receiving device for HGB ‘measurement. ‘None u RBCPLT bath 200100084 This consists of the front bath, and the rear bath, with an aperture in between; the sample ‘lows from the front bath through the aperture to generate electric pulses that facilitate RBC and PLT counting. ‘Negative-pres sure chamber 200100044 1. This chamber builds the negative pressure needed to drive impedance-channe! counting: [Link] chamber builds positive pressure needed to drive the mixing, 3. This chamber builds negative pressure needed to flush the rear bath of the impedance counting bath and to discharge the waste liquid in the counting bath. Copyright © SHENZHEN DYMIND BIOTECHNOLOGY CO.,LTD, 142 Instrument Configuration List of Tube Types No. [Tube Illustration Material [Inner |Outer __| Features Name No. Diameter | Diameter (mm) (mm) 1 |DoubleLa 163010006 |2.0 35 Double-layered with an yered Tube ecosion-resistant inner layer 2 |EVATube }63010007A |1.0 3 Has good flexibility ‘and resistance to bending and fatigue 3 [Thin so [63010008 ]1.6 32 Soft Tube Tube 4 |Thick 50 153010009 |2.4 4 Soft Tube Tube 5 [3603 Tube 63010010 |3.2 64 |A sof tube used for the external inflow of diluent and waste discharge 6 |MPF Tube je3o10011a |2 38 Acbard tube, resistant to pressure and ‘deformation 7 /15mm_ [63010013 |1.3 25 This tube is hard and ‘Teflon transparent, and is very Tube resistant to erosion 8 /63010004A |1.6 48 ‘This tube is soft and ‘ean be connected t0 hard tubes 9 [63010005a ]3.2 64 ‘This tube is soft and id) ‘can be connected to Silicone hard tubes; it can also Tube be used as a protective shell 10 |TPUTube [63010016A |24 48 A hard tube, resistant to pressure and ‘deformation ‘Copyright © SHENZHEN DYMIND BIOTECHNOLOGY CO., LTD. 182 Instrument Configuration No. [Tube Illustration Material [Inner |Outer__| Features Name No. Diameter | Diameter (mm) (mm) M1 |2.0mm 163010020 |2 4 This tube is hard, has id.) good resistance to ‘Teflon erosion, and can be Tube used as an aspiration abe inside the bottle of lyse List of Connector Types No. | Connector | Illustration ‘Material | Material Features Name No. 1 | T4201 53050003 | White nylon | T-type Connector for Connector 1.6-2.4mm (i.d.) Tubing 2 | 420-6005 53050005 | PP 10-32 serew-thread Connector ‘connector for 1.6-2.4mm (id) tubing 3 | 220-6005 53050008 | PP, natural — | 1/4-28 screw-thread Connector color connector for 1.6-2.4mm id.) tubing 4 | L401 53050010A | White nylon | L-type Connector for Connector 1.6-2.4mm (i.d.) Tubing 3 | ¥230-1 53050011A | White nylon | Yype Connector for Connector 24-3.2mm (i.d.) Tubing 6 | ¥a20-1 53050012A | White nylon | ¥-type Connector for Connector 1.6-2.4mm (i.d.) Tubing 7 | Na30/420-1 53050013A | White nylon | Thick-to-thin tube Connector connector for 1.6-2.4mm configuration 8 | N420-6005 53050014 | PP,natural | Barrel connector for Connector color 16-2.4mm (i.d.) bing Copyright © SHENZHEN DYMIND BIOTECHNOLOGY CO.,LTD, 162 Instrument Configuration 9 | MTLL230-11 tegrated locking ring 530S0016A | White nylon | Integrated locking ring 10 | MTLLo13-3 Integrated locking rit 53050044A | Red nylon | Integrated locking ring | LNs Locking bolt for panel installation 53050023 | White nylon | Locking bolt for white panel installation 12 | LNS3 Locking bolt for panel installation 530500254 | Red nylon —_| Locking bolt for red panel installation B | ccrR4 Color-coded locking ring 53050028A | White nylon | White-coded locking ring 4 | CCIRS Color-coded. locking ring 53050030A | Red nylon —_| Red-coded locking ring 2.2.9 Main Measurement Modes There are two measurement modes including whole-blood mode and predilute mode. [Link] Dilution Procedure in Whole-blood Mode Dilution procedure in whole-blood mode: Copyright © SHENZHEN DYMIND BIOTECHNOLOGY CO.,LTD, 72 Instrumant Configuration ion Procedure in Whole-blood Mode Whole-blood Mode ‘Sampling , Dispensing | DIL-E |}-——+| Diluted sample Diluted sample LYE-1 [pice | “RBC/PLT WBC/HGB measurement module Measurement module [Link] Dilution Procedure in Predilute Mode Dilution procedure in predilute mode: Figure 2-10 Dilution Procedure in Predilute Mode Predilute mode sampling ¥ [Dispensing] [DIL-E}-—+| y Diluted sample Diluted sample U r= RBCPLT WBCHGB measurement module | measurement module, Copyright © SHENZHEN DYMIND BIOTECHNOLOGY CO.,LTD, 182 Instrument Configuration [Link] Measurement Procedure in Whole-blood Mode In whole-blood mode, a total of S time slots for sample measurement are set at 0~4s, 5~175, 18~30s, 31-ds, 45-605, 32s, 45~485, 49-54, and 45~60s, respectively, during each of which different steps are taken. Detailed description: © Steps taken between 0~4s (Figure 2-11) 4. Aspirate the whole blood using the sample syringe. b, Set the sample probe in the upper position for outer-wall cleaning. Figure 2-11 Measurement Flowchart A Hydraulics Diagram © Steps taken between S~17s (Figure 2-12) Insert the sample probe into the WBC/HGB bath. b. The contaminated sample is removed from the tip of the sample probe from inserting it into the WBC bath, Drain the blood sample and diluent from the WBC/HGB bath, Add the diluent into the WBC/HGB bath. Add the blood sample into the WBC/HGB bath and mix it through with air bubbles. Drain the RBC/PLT bath. Add the base solution into the RBC/PLT bath. Copyright © SHENZHEN DYMIND BIOTECHNOLOGY CO.,LTD, 192 Instrument Configuration jure 2-42 Measurement Flowchart B Hydraulics Diagram © Steps taken between 1830s (Figure 2-13) a, Move the sample probe to the upper position and clean its inner and outer walls. ». Insert the sample probe into the WEC/HGB bath to aspirate the diluted sample. €. Move the sample probe to the upper position and above the RBC/PLT bath, and then into the RBC/PLT bath, d. Add the diluted sample into the RBC/PLT bath once with the sample probe, and mix well using the air bubbles. Add the LYE-1 lyse into the WBC/HGB bath and mix through with air bubbles. ‘Move the sample probe upward into the upper position and clean its outer wall, A pressure of -30Kpa is created in the negative-pressure chamber. Copyright © SHENZHEN DYMIND BIOTECHNOLOGY CO.,LTD, 202 Instrument Configuration jure 2-13 Measurement Flowchart © Hydraulics Diagram =o foes © steps taken between 31~445, Data of WBC channel and RBC channel is under measurement. © Steps taken between 45~60s (Figure 2-14) ‘Measurement of WBC channel and RBC channel has completed. Drain the WBC/HGB bath. Add the diluent into the WBC/HGB bath for cleansing. |. Zap the WBC/HGB bath. Zap the RBC/PLT bath. Drain the WBC/HGB bath before adding the diluent as the base solution, Drain the RBC/PLT bath before adding the diluent as the base solution. Clean the rear baths of the WBC/HGB and RBC/PLT baths. Restore the sample assembly to measurement preparation status, j. Ataround the 56s mark, the screen will show the current counting results. k. At around the 605 mark, the sample probe is set to the blood-sampling position, which indicates that it is ready to take the next sample. Copyright © SHENZHEN DYMIND BIOTECHNOLOGY CO.,LTD, a2 Instrument Configuration jure 2-14 Measurement Flowchart D Hydraulics Diagram [Link] Measurement Procedure in Predilute Mode The measurement principle in predilute mode is generally the same with that in whole-blood mode, while, the difference lies in that the whole-blood measured shall be quantitatively prediluted prior to the measurement in predilute mode. 2.2.10 Hydraulics Maintenance Swab Cleaning and Maintenance In the case of serious device contamination, our service team can provide on-site service either regularly or by request. The swab requires cleaning and maintenance to get rd of any contamination found on its lower surface, The operating procedure as follows: Shut down the device and disassemble the swab, A certain amount of probe cleanser is diluted with the diluent into a 1:3 (probe cleanser to diluent) solution. Repeatedly scrub the bottom end ofthe swab and the inside ofits lower aperture with a [Link] dampened with the diluted probe cleanser. After serubbing, use another clean Q-tip dampened with pure diluent to clean away the probe cleanser residue atthe bottom end of the swab and the inside of its lower aperture. See Figure 2-15 Copyright © SHENZHEN DYMIND BIOTECHNOLOGY CO.,LTD, 22 Instrument Configuration igure 2-15 Cleaning the swab After cleaning and reassembling the swab (and ensuring that the inflow/outflow interface tubing is connected correctly), turn on the device and go to Services > Clean. Double-click the Clean Sample Probe burton to perform the operation before performing cleaning and maintenance on the swab. Cleaning and Maintenance of the WBC Bath Log into the analyzer software interface and go to Services > Clean. Double click Clean WBC Bath. See Figure 2-16. Figure 2-16 Cleaning and maintenance interface ‘Clean gel Fe = © Clean WBC Bath Clean RBC Bath Clean Sample Probe Cleaning and Maintenance of the RBC Bath Log into the analyzer software interface and go to Services > Clean. Double click Clean RBC Bath. 2.2.11 Troubleshooting Common Hydraulics Problems [Link] Commonly Used Equipment and Tools Name Tllustration Usage Remarks a. 1. Used to contain liquid after a mbe is, palled out Small sample cup 2. Used as a dilvent container None 3. The ratio of probe cleanser to diluent is 13, 2 Copyright © SHENZHEN DYMIND BIOTECHNOLOGY CO.,LTD,2 Instrument Configuration Name Illustration Usage Remarks plastic syringe 1. Used to manually unclog the WBC and (disposal syringe RBC channels; None with no pillow) cs 2. Used to inject other liquids, Barrel connector tubing connection None Used to connect tbing and the plastic Silicone tube vis None 1. Used to serub and clean the swab. tasks. [Link] Inspection and Troubleshooting of Valve Clog: To prevent the possibility of liquid flowing onto the base panel when the tube connected to the valve is pulled out, the tube pulled out needs to be placed into the small sample cup, and tissue paper should be used as a pad to prop up any affected components. The sample cup should be removed once the liquid has stopped flowing. Disassemble the valve. Open the valve cap to identify any clogging caused by impurities After the impurities have been removed, place the components in theit original positions (note that the parts with instructions are kept on the same side during installation, as shown in Figure 2-17~Figure 2-21. Then remove the tissue paper and scrub and clean the bottom.) Figure 2-17 3-way valve (L) jure 2-18 2-way valve (L) Copyright © SHENZHEN DYMIND BIOTECHNOLOGY CO.,LTD, 242 Instrument Configuration Figure 2-19 Installation of the large valve Figure 2-20 Installation of the 3-way _ Figure 2-21 Installation of the 2-way valve (S) valve (S) = [Link] Liquid Pump Clogging: Inspection and Troubleshooting 1. Pull out the tubing and place it ito the small sample cup. Then prop up any relevant parts with tissue paper and disassemble the pump. Use te Tons screwdriver to open the pump cap to observe any clogging resulting from impurities, as shown in Figure 2-22. Figure 2-22 Pump cap 2, Remove the impurities and place the components in their original positions. Make sure to align the ‘two marked lines correctly while installing (as shown in Figure 2-23). Copyright © SHENZHEN DYMIND BIOTECHNOLOGY CO.,LTD, 22 Instrument Configuration Figure 2-23 Installing the marked lines 3. When reassembling the pump, make sure the Liquid inlet and outlet are connected to the correct tubing (eg. Figure 2-24), Figure 2-24 Symbols for inflow and outflow direction [Link] Aperture Clogging of the WBC Channel Aperture clogging can be resolved by implementing the procedure for cleaning the WBC bath and usin the aperture backflush. 1. Go to the Service» Maintenance > Clean screen in the analyzer software interface, and double-click the WBC bath button. 2. Goto the Service > Maintenance > Maintain screen, and double-click the Flush Apertures and then the zap Apertures button. After you are done, press the one-click troubleshooting button to check if the aperture has been unclogged. If clogging persists, the probe cleanser needs to be manually pushed through to clean the WBC-channel aperture, and the following steps should be taken 1. After obtaining permission from qualified service personnel, frst drain the WBC bath (the liquid can also be aspirated dry manually using a plastic syringe or other tools): Go to the Service > ‘Maintenance > Maintain screen, double-click the Empty WBC bath button (note that a prompt box will pop up for confirmation after draining: do not press the OK button, otherwise the WBC bath will be refilled with liquid), 2. Pull out the tubing connected to the WBC outflow tube and connect the plastic syringe filled with the Copyright © SHENZHEN DYMIND BIOTECHNOLOGY CO.,LTD, 262 Instrumant Configuration diluted probe cleanser (with a ratio of probe cleanser to diluent of 1:3) to the WBC outflow tube (as shown in Figure 2-25), Pushing the plastic syringe back and forth will facilitate the repeated flushing of the aperture with the probe cleanser. Apply proper force while pushing to prevent the tube from “Maintenance > Maintain screen, double-click the Empty WBC bath button (note that a prompt box will pop up for confirmation after draining: do not press the OK button, otherwise the WBC bath will be refilled with liquid), Disassemble the isolation chamber below the WBC bath and pull out the corresponding tubing. Use the thick No. 50 tube or 1.6mim (id,) silicone tube to attach the two connectors to the side of the Copyright © SHENZHEN DYMIND BIOTECHNOLOGY CO.,LTD, 282 Instrument Configuration isolation chamber, plugging both apertures. Then attach the plastic syringe to the connector above the isolation chamber using a 1.6mm (i.d.) silicone tube. Push the plastic syringe to pressurize the isolation chamber, and hold the syringe steady for 30 seconds to check for any appearance of air bubbles. If any air bubbles occur, the isolation chamber needs to be replaced, See the picture below Figure 2-27 Checking whether or not the isolation chamber is airtight Sealing tube used for closure 5, After inspection, the components and tubing need to be restored to their original locations. Click the ‘OK button in the confirmation box that pops up after the counting bath is drained by opening the user interface. Ifthe Liquid level in the WBC bath is found to be too high, immediately shut down the device to prevent any further overflowing. [Link] Overflowing of the RBC Bath The troubleshooting procedure is the same as that in [Link] Overflowing of the WBC Batt; the only difference is that valve to be inspected is Valve 11. The tubing for inspection is shown in Figure 2-28, and the isolation chamber to be inspected is the one below the RBC bath, Copyright © SHENZHEN DYMIND BIOTECHNOLOGY CO.,LTD, 292 Instrument Configuration Figure 2-28 RBC tubing of liquid discharge Hydraulics Diagram ayes [Link] Leakage from the Swab First wipe off any liquid with tissue paper, and then perform the following troubleshooting steps: 1. Check if Pump 1 is clogged. Follow the steps specified in [Link] Liguid Pump Clogging: Inspection and Troubleshooting t0 inspect and resolve any clogging of the liquid pump. After the troubleshooting, run the whole-blood counting procedures to see if the problem as been resolved. 2. Check if Valve 8 is working normally. Follow the steps specified in [Link] Inspection and Troubleshooting of Valve Clogging to inspect. and resolve any clogging of Valve 8. After the troubleshooting, run the counting procedure to see if the problem has been resolved. Tf the problem has been resolved, end the procedure; ifthe problem persists, continue to perform the following steps: 3. Check if there are any kinks or damage along the tubing, Check if there are any kinks or damage along the tubing, as highlighted in red in Figure 2-29, and replace the corresponding tubing if the problem persists. After the troubleshooting, run the ‘whole-blood counting to see ifthe problem has been resolved. Copyright © SHENZHEN DYMIND BIOTECHNOLOGY CO.,LTD, 302 Instrument Configuration Figure 2-29 Swab tubing for fluid aspiration Hydraulics Diagram eos Ifthe problem has been resolved, end the procedure: if the problem persists, continue to perform the following steps: 4, Check if there is perforation at the bottom of the sample probe Set the small sample cup below the probe. When pulling out the tube above the probe (push the tube end outward to pull it out; otherwise there will be crimping in the sampling tube connected to the end of the probe), disassemble the probe, and connect it to the plastic syringe filled with the diluent using 4 1.6mm (i.d.) silicone tube. Manually push the syringe to see if any liquid flows from the bottom end of the probe. Ifsuch a flow is detected, this indicates that perforation exists at the bottom of the probe (the liquid normally flows from the sides. Be sure to collect the waste when pushing the liquid to the correct location, as shown in Figure 2-30). Copyright © SHENZHEN DYMIND BIOTECHNOLOGY CO.,LTD, a2 Instrument Configuration Figure 2-30 Check the liquid pushing of the sample probe Barrel connector. 1.0mm(d) silicone tube The liquid is spitted laterally under nermal conditions Liquid is pushed upward Restore the tubing to its original status (cut off the crimped part at the front end of the sampling tube, and readjust the tubing for installation. Make sure to check if the sampling tubing gets into the way of other assemblies whenever the sampling assembly moves up and down, or if it feels too tight. If this is the case, continue to adjust the sampling tubing until it looks like it fits properly.) 5. Che for any wear and tear in the top aperture of the swab ‘To disassemble, take off the swab’s fixed jump ring. Pull out the swabs inflow and outflow tubes. ‘Measure the upper-section aperture with a caliper; an aperture with a diameter of mote than 1.7Lmm indicates that the swab needs to be replaced. ‘When reinstalling or replacing the swab, fist put the inflow tube and outflow tube in place (Note: The inflow tube is the Thin 50 tube, connected to the bottom connector; the outflow tube iste Thick 50 ‘be, connected to the top connector), then put on the swab (insert the probe into the swab aperture) and tap the swab’s fixed jump ring to complete the procedure. [Link] Problems with Creating Positive Pressure The troubleshooting steps are 1. Check ifthe pressure chamber is broken: Disassemble the pressure chamber if necessary and seal the ‘openings with rubber tubes. Then place the pressure chamber into the water tank. Use a syringe or other equipment to pressurize the pressure chamber. Any air bubbles detected indicate that the pressure chamber needs to be replaced. 2. Check if there are any kinks or damage along the tubing to the pressure chamber. Any replacements should be made using tubing of the proper length and type Copyright © SHENZHEN DYMIND BIOTECHNOLOGY CO.,LTD, 322 Instrument Configuration Figure 2-34 Tubing of the positive-pressure chamber Hydraulics Diagram me 3. Check if the pump is working: if not, replace the pump. 4. Check if the positive-pressure sensor and the corresponding electrical circuit are in normal working order. First replace a reagent testing panel and reconnect its tubing, then check if the positive pressure normal in the status interface: if itis normal after the replacement, this means that the positive-pressure sensor and the corresponding electrical citcuit are causing a problem with building positive pressure (After the reagent testing panel is replaced, note that the tubing needs to be connected correctly. First keep a record of where it was by marking it before disassembling the tubing). 5. Check the valve directly connected to the tubing of the positive-pressure chamber to see 1) whether the on-off switeh is normally: and 2) whether there is any clogging 6. After troubleshooting, complete the steps shown in the one-click troubleshooting wizard. [Link] Problems in Creat 9 the Negative Pressure The troubleshooting steps are: 1. Check if the negative-pressure chamber is broken: Disassemble the negative-pressure chamber if necessary and seal the openings with rubber tubes. Then put the negative-pressure chamber into the water tank. Use a syringe or other equipment to pressurize the negative-pressure chamber. Any air bubbles that occur indicate that the negative-pressure chamber needs to be replaced. 2. Check if there are any kinks or damage along the tubing connected to the negative-pressure chamber, Any replacements should be made using tubing of the proper length and type. Copyright © SHENZHEN DYMIND BIOTECHNOLOGY CO.,LTD, 332 Instrument Configuration Figure 2-32 Tubing of the negative-pressure chamber Hydraulics Diagram 3. Check if Liquid Pump 2 is working: if not, replace the pump. 4. Check if the negative pressure sensor and the corresponding electrical circuit are in normal working order. First replace a reagent testing panel and reconnect its tubing: then check ifthe negative pressure is normal in status interface. If is normal after the replacement, this means thatthe negative-pressure sensor and the corresponding electrical circuit have caused a problem with building negative pressure (Affer the reagent testing panel is replaced, note thatthe tubing needs to be connected correctly. First keep a record of where it was by marking it before disassembling the tubing). 5. Check the valve directly connected to tubing of the negative-pressure chamber to see 1) whether the on-off switch is normally: and 2) whether there is any clogging 6. After troubleshooting, complete the steps shown in the one-click troubleshooting wizard. 2.3 Hardware System The hardware system of the Auto Hematology Analyzer consists of the main control panel, reagent testing panel, front panel, reagent sealing panel, etc. and its block diagram is shown in Figure 2-33, Copyright © SHENZHEN DYMIND BIOTECHNOLOGY CO.,LTD, 342 Instrument Configuration Figure 2-33 Block Diagram of Hardware System Display screen || Front panel (LED, driver panel buzzer) an . pipe Becket Closing Panel (—”) Main Control Panel Testing Panel it Other external connecting unit (valve, pump, motor, sensor) Hardware maintenance principles apply to all the panels. Al the panels have passed hardware tests before Jeaving the factory, and any hardware problem found Icater may be mainly caused by power problems. Thus, hardware maintenance primarily involves the power supply. Hardware problems and maintenance methods for each panel are described in the subsequent sections. 2.3.1 Main Control Panel The main control panel is the core panel of the DH hematology analyzer; the device cannot work properly with a problematic main control panel. This section introduces the main control panel from the perspectives of composition, problem identification, and maintenance. Copyright © SHENZHEN DYMIND BIOTECHNOLOGY CO.,LTD, 362 Instrument Configuration [Link] Composition of the Main Control Panel Figure 2-34 Block Diagram of Hardware System Functions ‘Network imerface, LCD screen (user External analog interface USB interface interface) jj oF 2 External digital interface z t ey TOL m8 & comatatcton| M aa ARMunit |(———)>) ‘| A data acquisition part PFGA unit | |__» Digital power » ==) Analog control signal supply Motor — dl Valve, 2 9g ave, pamp K—=| © [isierene =) g a z| [eg [2 Pressure signal | ae 8] [2 g : Reagent testing -—} 2 KX) BF g| \eel | [Micoawiek -—) * 2| BBE | Temperature control 5 ~ The block diagram above gives a brief overview of the composition of main control panel and the function of each module. Further description can be found below based on the pictures for each panel. Picture of main control panel is shown in Figure 2-35, Copyright © SHENZHEN DYMIND BIOTECHNOLOGY CO.,LTD,2 Instrument Configuration jure 2-35 Main control panel 2 8 3 7 4 5 6 1 - Extemal wiring interface 2-Network interface and USB interface 3 - Digital circuit module 4~ Debugging interface 5 - Motor, pump valve and 6 Analog impedance channel module temperature-control module 7 - Voliage-multiplying circuit module | 8 - Analog HGB channel module The main control panel can be clearly divided into digital part, analog part and driver part. Among them: © The left half of the panel is the digital part, including ARM unit, FPGA unit, extemal interface, debugging interface, power supply, etc. © The right half of the panel is the analog par, including impedance channel, hardware monitoring channel, external interface, power supply, etc. © The lower left comer of the panel is the driver par, including motor driver, valve & pump driver, ‘temperature contro, ete © Inthe middle of the panel there are AD and interface chips, where AD is responsible for analog-to-digital conversion and the interface chip is responsible for on-off control for relevant functions of the analog part [Link] Peripheral Interface of the Main Control Panel There are several interfaces on the main control panel, and the detailed interface description is shown in Table 2-1 Copyright © SHENZHEN DYMIND BIOTECHNOLOGY CO.,LTD, Ea2 Instrument Configuration 1 R RBC analog signal interface 2 mu ‘Motor driver interface 3 22 ‘Motor driver interface 4 23 ‘Valve driver interface 5 pa Valve driver interface 6 sy Power supply input interface 7 BT Pump driver interface 8 B9 ‘Heating rod control interface 9 340 Heating rod control interface 10 a ‘MPU_ITAG interface i 2 FPGA_ITAG interface 12 3 FPGA_AS interface 13 BL Pressure sensor signal interface 4 BS Front display panel interface 15 43 ‘Network interface 16 346 USB interface 7 7 USB interface 18 m4 ‘Back-light panel interface 1 44 Display sereen data communication interface 20 3 Touch screen data interface 21 a Serial port 2 is Serial port B 8 Printer interface 24 326 ‘Reagent testing signal interface 2s D7 ‘Microswitch signal interface 26 28 Motor position optocoupler signal interface 27 m9 ‘Motor position optocoupler signal interface 28 BO ‘Temperature sensor interface 29 8 SV power inlet 30 ng 3V power inlet 31 320 SV power outlet Copyright © SHENZHEN DYMIND BIOTECHNOLOGY CO.,LTD,2 Instrument Configuration No. TagNo. | Description 32 49 SV power outlet 3 350 12V power outlet 34 mt GB signal interface 35 H10 #12V power inlet 36 s16 110V burning power input interface 35 is the socket for the lead wire of the RBC bath, while J6 is the socket for the lead wire of the WBC bath. The troubleshooting interfaces designated for the miain control panel are serial interface 17 (RS232 signals), FPGA JTAG interface J3, FPGA AS interface J4, and SD interface for programming before shipping. Use of these troubleshooting interfaces is restricted to developers only. [Link] Power input and indicator lights on the Main Control Panel Digital Power Input and Indicator Lights Power input of the digital partis SV, and it can be tested via testing point TP1S (nuultimeter shall be adopted for testing, and during the testing, the red probe shall be connected to TPIS, while the black probe shall be connected to the metal part on the housing). The SV power input is accompanied by the fuse F2, Normally the fuse will not be blown since the power supply of the device is equipped with surge protection. Ifa short circuit occurs under the input of SV of power, the power supply will fail to work properly and a buzzing sound can be heard. All digital power supplies of the main control panel are equipped with an indicator light and testing point and corresponding relationships between the digital power supply, indicator light, and testing point is, shown in the table below. Position Code of | Meaning ‘Normal Status Indicator Light pai SV digital power supply on DIT 3.3V digital power supply on Dis 2.5V digital power supply on Dis L SV digital power supply on Dis TPS65910 3.3V working status! on NOTE: 1- D1 usually indicates the wouking status ofthe ARM power management chip, TPS6S910A31AL, but this indicator light oaly indicates the working status of oue LDO signal channel in TPS65910; the multimeter can be used to test the couresponding testing polats for other LDO and DC-DC channels. Fr details, see "Digital Testing Points" in the section of [Link] Testing Points on the Main Control Panel Copyright © SHENZHEN DYMIND BIOTECHNOLOGY CO.,LTD, 392 Instrument Configuration Analog Power Input and Indicator Lights The analog power input of +12V and -12V on the main control panel can be tested for actual voltage values via the testing points TP21 and TP22, The +12V power input is accompanied by the fuse F4, while -12V is accompanied by the fuse FS. Like the SV digital power input, +12V and -12V derive power from the device's power supply: if both are subject to overload, they will receive surge protection from the power source and the corresponding indicator lights will not be activated. Tie following table ists the analog power indicator lights Position Code of Meaning, Normal Status Indicator Light Dis +12V power indicator light on pis / power indicator light on 23 -5V power indicator light on D2s +5V power indicator light On 'V and -SV originate from the linear stabilizer. The corresponding lights will not be activated by the 'V or -SV overload, and the linear stabilizer will be very hot. If either the +5V or the -SV power indicator light is not on, immediately power off the device and troubleshoot the corresponding electrical citeuits. We recommend replacing the main control panel Other Indicator Lights on the Main Control Panel ‘The ARM and FPGA units are each assigned a single indicator light to show their working status on the main control panel, In addition, a USBO power indicator light is designated for the ARM unit. The following table lists the indicator lights Position Code of | Meaning ‘Normal Status Indicator Light D79 It indicates the ARM working status and flashes | Flashing after proper system loading, 90 It indicates the FPGA working status and flashes | Flashing after proper system loading. Ds2 It indicates the MPU working status and flashes | Flashing after proper system loading. Indicator lights are very useful in practice: hardware problems can be identified by the status ofthe indicator lights. For details, see [Link] Identification of Main Control Panel Problems. [Link] Testing Points on the Main Control Panel There are two types of testing points on the main control panel: Digital and analog. | Testing Points The digital testing points on the main control pane! listed here are commonly used ones, in particular for Copyright © SHENZHEN DYMIND BIOTECHNOLOGY CO.,LTD, 402 Instrument Configuration power supply and key signals: Position Code of | Description Testing Points TPIS the testing point for SV digital input, with the expected voltage of SV TP9 ‘TPS65910 VRC output, with the expected voltage of 1.8V TP6L DDR3 reference voltage, with the expected value of 0.75V P7 ‘TPS65910 VDIG1 output, with the expected value of 1.8V TPS ‘TPS6S910 VDIG2 output, with the expected value of 1.8V TP6 ‘TPS65910 VAUX33 output, with the expected value of 33V TRS ‘TPS65910 VMMC output, with the expected value of 3.3V TPS ‘TPS65910 VAUX2 output, with the expected value of $3V Tra ‘TPS65910 VAUXI output, with the expected value of 1.8V TPL ‘TPS65910 VDAC output, with the expected value of 1.8V ‘TP2 ‘TPS6S910 VPLL output, with the expected value of L8V TP20 the testing point for 3.3V digital power supply, with the expected voltage of 33V TPI9 the testing point for 2.5V digital power supply. with the expected voltage of 2.5V TPIT the testing point for 1.2V digital power supply, with the expected voltage of 12V TPIS the testing point for 1.8V digital power supply, with the expected voltage of 1.8V Ls CLK DIFF low-angle AD clock 4MHz clock signal MS_CLK DIFF medium-angle AD clock, 4MH? clock signal HS_CLK DIFF high-angle AD clock, 4MHz clock signal WBC_CLK WBC-channel AD clock, IMFz clock signal RBC_CLK RBC-channel AD clock, IMHz clock signal Analog Testing Points The following table lists the analog testing points; Position Code | Description of Testing, Points Tes AVCC_+5V testing point, with the expected voltage of SV TP2L AVCC_+12V testing point, with the expected voltage of +12V Copyright © SHENZHEN DYMIND BIOTECHNOLOGY CO.,LTD, 42 Instrument Configuration Position Code | Description of Testing Points P22 AVCC_-12V testing point, with the expected voltage of -12V. ‘TP2s AVCC_-SV testing point, with the expected voltage of -SV TRss HGB_LED_CTL testing point, HGB light-driven control switch: O for on and 1 for off P47 WBC channel first-level OPAMP output testing point TPas WBC channel thitd-level OPAMP output testing point TP4s WBC channel fourth-level OPAMP output testing point TRad WBC channel sixth-level OPAMP output testing point ‘TPas WBC channel seventh-level OPAMP output testing point WBC_AD WBC-channel AD front-end testing point TP39 RBC channel first-level OPAMP output testing point ‘TP4o RBC channel third-level OPAMP output testing point ‘TP38 RBC channel fourth-level OPAMP output testing point TPaL RBC channel sixth-level OPAMP output testing point ‘TPa2 RBC channel eighth-level OPAMP output testing point RBC_AD RBC-channel AD front-end testing point TP31 SELECT_WBC_CTL testing point, the switch control signal for WBC bath zap and CC source: 0 for zap and 1 for CC source TP29 SELECT_RBC_CTL testing point, the switch control signal for RBC batit zap aiid CC source: 0 for zap and 1 for CC source TPs3 VCONST_MON_AD testing point, 1.36V+0.2V TP2s RH_MON testing point, 1.9V20.2V TP30 WH _MON testing point, .720.2V ‘P33 VCONST_CTL testing point, the switch control signal for CC source: 0 for on and | for off LSIN the testing point for DIFF low-angle signal input Is_AD DIFF low-angle AD front-end testing point MSIN the testing point for DIFF medium-angle signal input Ms_AD DIFF medium-angle AD front-end testing point HSIN the testing point for DIFF high-angle signal input Hs_AD DIFF high-angle AD front-end testing point HGB_AD HGB-channel AD front-end testing point Copyright © SHENZHEN DYMIND BIOTECHNOLOGY CO.,LTD, 422 Instrument Configuration Position Code | Description of Testing Points P37 LASER_MON_AD testing point, reflecting the laser current TP36 AVCC_+12VMON_AD testing point, reflecting the voltage of AVCC_+12V power source ‘TP3s AVCC_-12VMON_AD testing point, reflecting the voltage of AVCC_+12V power source [Link] Identification of Main Control Panel Problems Problems with the main control panel can be categorized as power problems, connection problems, and functional problems. Power problems can be identified using the status of power indicator lights, and connection problems can identified by way of direct observation; however, functional problems area little tricky, and can be indirectly shown using other indicators. Power Problems of the Main Control Panel and their Indicators ‘The power supply of the main control panel is clearly divided between digital and analog. Among them: © A digital power input of SV panel, as shown in Figure 2-36. Figure 2-36 Digital Power Input, Testing Point and Indicator Light nabled by the upper middle J18, J19 interfaces on the main control 4 freee | T Le | 3 2 1 - SV power inlet 2 - testing point for SV power supply 3 - SV power indicator light © Ananalog power input of +12V and -12V is enabled by the upper right J10 interface on the main control panel, as shown in Figure 2-37. Copyright © SHENZHEN DYMIND BIOTECHNOLOGY CO.,LTD, 432 Instrument Configuration 1 - 412V power inlet 3 12V testing point © A power input of 12V and 24V is enabled by the lower left J17 interface on the main control panel. as shown in the figure below. 1- 12V and 24V power input interface 24V testing point 3- 12V testing point All power inputs on the main control panel derive from the power input of the device. If either of the power inputs on the main control panel receives a surge from a grounded or any other input power source, the device's power supply will activate its automatic protection mechanism and produce a buzzing sound. For the normal status of power indicator lights shown in the figures above, please refer to the descriptions of the power supply and indicator lights of the main control panel in [Link] Composition of the Main Control Panel. Ifthe power indicator light is in a status different from normal, this means that the power supply is not working properly. ULI deserves further description as follows. ULI is the ARM power management chip in the digital Copyright © SHENZHEN DYMIND BIOTECHNOLOGY CO.,LTD, “42 Instrumant Configuration section of the main control panel. The working status of this chip determines the working status of the ‘main control panel. As shown in Figure 2-38, D14 tumed on indicates the proper working of ULL. Figure 38 ARM power management chip in the main control panel 1 - Power management chip UI | 2- UI working indicator Functional Problems with the Main Control Panel and their Indicators Functional problems with the main control panel are typically related to one of two causes. One cause may be that the counting function of the main control panel cannot be realized, For example, the digital ppart’s operating system on the main control panel is not operational, the FPGA program does not load, or there is abnormal counting: the other cause refers to control failure of the motor, valve-pump, temperature control, ete © ARMOS on the main control panel fails t0 boot ‘The ARM on the main control panel boots from NAND using its default factory setting, so the booting media selection switch $6 should be turned to NAND (ie. OFF state for DIP switches), as shown in Figure 2-39 Figure 2-39 ARM booting and running L-DIP switch status 2- ARM normal working indicator light D79 ‘When the ARM OS is booted properly, the working-status indicator light D79will flash on the main Copyright © SHENZHEN DYMIND BIOTECHNOLOGY CO.,LTD, 452 Instrument Configuration control panel. If D79 is not lit up it means that the ARM OS has failed to boot. © MPU program loading fails ‘When the MPU program is booted properly, the working-status indicator light D52 will flash on the ‘main control panel. If DS2 is not lit up, it means that the MPU program has failed to boot. The location of DS2 is shown below. Aa © Loading problems with the FPGA program ‘The FPGA program is loaded from the serial port FLASH. successful loading of the FPGA will cause the indicator light D90 to flash. If D90 is not Lit up, it means that FPGA program has not loaded. ‘The location of D90 is shown in Figure 2-40. Figure 2-40 Indicator of FPGA working status ue 1 - FPGA status indicator light © Counting abnormalities, ‘There are many causes that lead to counting abnormalities, and they can include problems with main control panel problem. Counting abnormalities caused by main control panel problems usually involve counting results of zero or overly high counting results on multiple occasions. A counting result of zero usually results from a failure to apply the CC source to the counting bath, while an overly high counting result is usually caused by too much noise in the analog-channel circuit. Copyright © SHENZHEN DYMIND BIOTECHNOLOGY CO.,LTD, 46