a) Historiales térmicos b) 3 zonas del proceso térmico

Tiempo (min) T1 (°C) T2 (°C) T3 (°C) T4 (°C) T5 (°C) calentamiento

0 30.7 27.5 25 28.6 25.2
0.5 31.8 30.4 27.5 29.7 26.4
1 36.7 41.2 28.8 34.7 28.7 T1 (°C)
1.5 44 54.4 32.1 42 29.8 140
2 52.5 63.9 36.5 50.5 31.6 120
2.5 59.6 69.8 40.9 57.7 33.7 100
3 66.4 74.3 47.1 64.5 36.3 80 f(x) = 0.0132073544433094 x + 94.67460674
R² = 4.81937690600676E-05
3.5 71.6 78.3 53.4 69.7 38.6 60
4 76.4 81.9 59 74.5 43.4 40
4.5 80.3 84.7 63.6 78.5 48.3 20
5 83.7 87.1 67.9 81.9 53.1
0
5.5 86.7 89.7 72.5 84.9 57.7 0 5 10 15 20 25 30
6 89.5 91.9 76.3 87.7 62.1
6.5 92.3 93.3 79.1 90.5 65.6
7 94 95.3 82.6 92.2 70 T2 (°C)
7.5 96.3 96.7 85.5 94.5 73.5 120
8 98 98 87.6 96.2 76.8 100
8.5 99.6 99.4 90.1 97.8 80 f(x) = 0.0121552604698674 x + 94.81910112
80 R² = 5.1214892475282E-05
9 101.3 100.7 92.7 99.6 83
60
9.5 102.7 101.8 94.2 101 85.7
10 103.7 102.7 96 102 88.2 40
10.5 104.6 103.6 97.7 102.9 90.6 20
11 105.5 104.2 98.7 103.8 92.3 0
11.5 106.3 104.7 99.9 104.6 94.1 0 5 10 15 20 25 30

12 107.1 105.4 101 105.4 95.9

12.5 107.9 106.1 102.1 106.2 97.6
13 108.5 106.5 102.9 106.8 98.6 T3 (°C)
13.5 108.9 106.7 103.7 107.2 99.7 140
14 109.4 107.1 104.4 107.7 101 120
14.5 109.9 107.5 105.1 108.2 102.1 100
15 110.5 107.9 105.9 108.8 103.1 80 f(x) = 0.0167381681988425 x + 86.5092883
15.5 110.9 108.3 106.5 109.2 104.2 R² = 6.0411006225225E-05
60
16 111.3 108.6 107.1 109.6 105.1 40
16.5 111.7 108.9 107.7 110 105.9 20
17 112.1 109.4 108.4 110.4 106.7 0
17.5 112.6 109.7 108.8 110.9 107.5 0 5 10 15 20 25 3
18 112.9 110.1 109.3 111.2 108.2
18.5 113.2 110.3 109.6 111.5 108.7
19 113.6 110.6 110.1 111.9 109.3 T4 (°C)
19.5 113.9 110.9 110.5 112.2 109.8 140
20 114.2 111.2 110.8 112.5 110.3 120
20.5 114.5 111.4 111.1 112.8 110.8 100
21 114.8 111.6 111.5 113.1 111.3 80 f(x) = 0.0132073544433096 x + 92.89820224
R² = 4.77575499491234E-05
60
40
20
0
 140
120
100
80 f(x) = 0.0132073544433096 x + 92.89820224
R² = 4.77575499491234E-05
21.5 115 111.9 111.9 113.3 111.7 60
22 115.2 112.1 112.1 113.5 112.1 40
22.5 115.4 112.3 112.4 113.7 112.5 20
23 115.6 112.5 112.6 113.9 112.8
0
23.5 115.8 112.7 112.8 114.1 113.1 0 5 10 15 20 25 30
24 116 112.8 113 114.3 113.3
24.5 116.1 113 113.3 114.4 113.6
25 116.3 113.2 113.5 114.6 113.9 T5 (°C)
25.5 116.4 113.3 113.7 114.7 114.2 140
26 116.6 113.4 113.9 114.9 114.4 120
26.5 116.8 113.6 114 115.1 114.6 100
27 116.9 113.7 114.2 115.2 114.8 80
f(x) = 0.022737487231869 x + 80.732359550
27.5 117 113.8 114.4 115.3 115 60 R² = 9.49192680324007E-05
28 117.1 113.9 114.6 115.4 115.2 40
28.5 117.2 114.1 114.7 115.5 115.4 20
29 110.5 107.9 105.9 108.8 103 0
29.5 109.9 107.5 105.1 108.2 102.1 0 5 10 15 20 25 30

30 109.4 107.1 104.4 107.7 101

30.5 108.9 106.7 103.7 107.2 99.7
31 108.5 106.5 102.9 106.8 98.6
31.5 107.9 106.1 102.1 106.2 97.6
32 107.1 105.4 101 105.4 95.9
32.5 106.3 104.7 99.9 104.6 94.1
33 105.5 104.2 98.7 103.8 92.3
33.5 104.6 103.6 97.7 102.9 90.6
34 103.7 102.7 96 102 88.2
34.5 102.7 101.8 94.2 101 85.7
35 101.3 100.7 92.7 99.6 83
35.5 99.6 99.4 90.1 97.8 80
36 98 98 87.6 96.2 76.8
36.5 96.3 96.7 85.5 94.5 73.5
37 94 95.3 82.6 92.2 70
37.5 92.3 93.3 79.1 90.5 65.6
38 89.5 91.9 76.3 87.7 62.1
38.5 86.7 89.7 72.5 84.9 57.7
39 83.7 87.1 67.9 81.9 53.1
39.5 80.3 84.7 63.6 78.5 48.3
40 76.4 81.9 59 74.5 43.4
40.5 71.6 78.3 53.4 69.7 38.6
41 66.4 74.3 47.1 64.5 36.3
41.5 59.6 69.8 40.9 57.7 33.7
42 52.5 63.9 36.5 50.5 31.6
42.5 44 54.4 32.1 42 29.8
43 36.7 41.2 28.8 34.7 28.7
43.5 31.8 30.4 27.5 29.7 26.4
44 30.7 27.5 25 28.6 25.2
 b) 3 zonas del proceso térmico c) velocidad de Tabla 1. Contenido residual de com
mantenimiento enfriamiento calentamiento
T más alta Tratamiento
T1 (°C/min) 0.0132 Ácido
T1 (°C) Ascórbico
(μg/mg)
Clorofila
(mg/g)
x) = 0.0132073544433094 x + 94.6746067415731 Firmeza (N)
² = 4.81937690600676E-05

a) Velocid
T (°C)
117.2
5 10 15 20 25 30 35 40 45 50 114.1
114.7
T2 (°C/min) 0.0122 115.5
T2 (°C) 115.4

Para cálculo de Ea - ácido

(x) = 0.0121552604698674 x + 94.8191011235955 T (K)
² = 5.1214892475282E-05
390.35
387.25
387.85
388.65
388.55
5 10 15 20 25 30 35 40 45 50

ácido a
T3 (°C) 0.0167 0
T3 (°C) 0.00256
-0.5
0.002565 0.002

-1
-1.5
-2
ln K

f(x) = 0.0167381681988425 x + 86.5092883895131 -2.5

R² = 6.0411006225225E-05 -3
f(x) = − 41650.300571
-3.5 R² = 0.6464500100150
-4
-4.5
5 10 15 20 25 30 35 40 45 50

T4 (°C) 0.0132
T4 (°C)

x) = 0.0132073544433096 x + 92.898202247191
² = 4.77575499491234E-05
x) = 0.0132073544433096 x + 92.898202247191
² = 4.77575499491234E-05

5 10 15 20 25 30 35 40 45 50

T5 (°C) 0.0227
T5 (°C)

x) = 0.022737487231869 x + 80.7323595505618
² = 9.49192680324007E-05

5 10 15 20 25 30 35 40 45 50
bla 1. Contenido residual de componentes termolébiles y firmeza antes y después del proceso
de esterilización
Control (ST) T1 T2 T3 T4 T5
m= 41650
112.1 85.8 101.4 97.5 90.3 95.6

m= 34809
256.45 201.3 231 222.2 205.7 213.6

45.34 37.4 42.5 41 38.7 40.1 m= 45671

a) Velocidad de reacción y energía de activación

K ácido K clorofila K firmeza Ea ácido Ea clorofila Ea firmeza
0.0411342036 0.0372519048 0.0296182986 J/mol J/mol J/mol R (J/mol)
0.0154335752 0.0160793851 0.009951626 346278.1 289402.026 379708.694 8.314
0.0279077904 0.0286711673 0.0201235157
0.0332698261 0.0339253719 0.0243615454 kJ/mol kJ/mol kJ/mol
0.0398046275 0.0457071517 0.0307033277 346.2781 289.402026 379.708694

Para cálculo de Ea - ácido Para cálculo de Ea - clorofila Para cálculo de Ea - firmeza

1/T ln K T (K) 1/T ln K T (K) 1/T ln K
0.0025618035 -3.190915299 390.35 0.0025618 -3.2900522 390.35 0.0025618 -3.51936291
0.0025823112 -4.171209934 387.25 0.00258231 -4.13021725 387.25 0.00258231 -4.61001932
0.0025783164 -3.578849403 387.85 0.00257832 -3.55186328 387.85 0.00257832 -3.90586621
0.0025730091 -3.403104416 388.65 0.00257301 -3.38359211 388.65 0.00257301 -3.7147494
0.0025736713 -3.223772104 388.55 0.00257367 -3.0855005 388.55 0.00257367 -3.48338424

ácido ascórbico Clorofila

0 0
0.00256 0.002565 0.00257 0.002575 0.00258 0.002585 0.00256 0.002565 0.00257 0.002575 0.00258 0.002585
-0.5 -0.5
-1 -1
-1.5 -1.5
-2 -2
ln K

ln K

-2.5 -2.5
-3 -3
f(x) = − 41650.3005712924 x + 103.6869022971 f(x) = − 34809.2169251258 x + 86.1044937941828
-3.5 R² = 0.646450010015058 -3.5
R² = 0.457587133512744
-4 -4
-4.5 -4.5
1/T 1/T
 b) Constantes cinéticas c) cál
T (°C) D ácido D clorofila D firmeza tiempo (min)
117.2 55.9874702 61.822342 77.7559857 0
114.1 149.220123 143.22687 231.419468 0.5
114.7 82.5217606 80.3245984 114.443223 1
115.5 69.2218827 67.8842964 94.5342327 1.5
115.4 57.8575946 50.3859881 75.0081562 2
2.5
Z Q10 3
Ácido 8.42533736 15.3854415 3.5
Clorofila 10.0811658 9.82036223 4
Firmeza 7.68354757 20.0316473 4.5
5
5.5
6
6.5
7
7.5
8
8.5
9
9.5
10
10.5
11
11.5
Firmeza 12
0 12.5
2575 0.00258 0.002585 0.00256 0.002565 0.00257 0.002575 0.00258 0.002585
-0.5 13
-1 13.5
-1.5 14
-2
14.5
ln K

-2.5
15
-3
-3.5 15.5
86.1044937941828 f(x) = − 45670.9403932028 x + 113.702208556439
-4 R² = 0.587579012991192 16
-4.5 16.5
-5 17
1/T 17.5
18
18.5
19
19.5
20
20.5
21
21.5
22
22.5
23
23.5
24
24.5
25
25.5
26
26.5
27
27.5
28
28.5
29
29.5
30
30.5
31
31.5
32
32.5
33
33.5
34
34.5
35
35.5
36
36.5
37
37.5
38
38.5
39
39.5
40
40.5
41
41.5
42
42.5
43
43.5
44
 c) cálculo de letalidad
T1 T2 T3 T4 T5 tiempo referencia:
9.33254301E-10 4.46683592E-10 2.51188643E-10 5.7544E-10 2.63026799E-10 121
1.20226443E-09 8.7096359E-10 4.46683592E-10 7.4131E-10 3.4673685E-10 z= 10
3.71535229E-09 1.04712855E-08 6.02559586E-10 2.34423E-09 5.88843655E-10
1.99526231E-08 2.18776162E-07 1.28824955E-09 1.25893E-08 7.58577575E-10
1.41253754E-07 1.9498446E-06 3.54813389E-09 8.91251E-08 1.14815362E-09
7.2443596E-07 7.58577575E-06 9.77237221E-09 4.67735E-07 1.86208714E-09
3.4673685E-06 2.13796209E-05 4.07380278E-08 2.23872E-06 3.38844156E-09
1.14815362E-05 5.37031796E-05 1.73780083E-07 7.4131E-06 5.75439937E-09
3.4673685E-05 0.00012302688 6.30957344E-07 2.23872E-05 1.73780083E-08
8.51138038E-05 0.00023442288 1.81970086E-06 5.62341E-05 5.37031796E-08
0.00018620871 0.00040738028 4.89778819E-06 0.00012303 1.6218101E-07
0.00037153523 0.00074131024 1.41253754E-05 0.00024547 4.67735141E-07
0.00070794578 0.00123026877 3.38844156E-05 0.00046774 1.28824955E-06
0.00134896288 0.00169824365 6.45654229E-05 0.00089125 2.8840315E-06
0.00199526231 0.0026915348 0.00014454398 0.00131826 7.94328235E-06
0.00338844156 0.00371535229 0.00028183829 0.00223872 1.77827941E-05
0.00501187234 0.00501187234 0.00045708819 0.00331131 3.80189396E-05
0.0072443596 0.00691830971 0.00081283052 0.0047863 7.94328235E-05
0.01071519305 0.00933254301 0.00147910839 0.00724436 0.00015848932
0.01479108388 0.01202264435 0.00208929613 0.01 0.00029512092
0.01862087137 0.01479108388 0.00316227766 0.01258925 0.00052480746
0.02290867653 0.01819700859 0.00467735141 0.01548817 0.00091201084
0.02818382931 0.02089296131 0.00588843655 0.01905461 0.00134896288
0.03388441561 0.02344228815 0.00776247117 0.02290868 0.00204173794
0.04073802778 0.02754228703 0.01 0.02754229 0.00309029543
0.04897788194 0.03235936569 0.01288249552 0.03311311 0.0045708819
0.05623413252 0.03548133892 0.01548816619 0.03801894 0.00575439937
0.06165950019 0.03715352291 0.01862087137 0.04168694 0.00741310241
0.06918309709 0.04073802778 0.02187761624 0.04677351 0.01
0.07762471166 0.04466835922 0.02570395783 0.05248075 0.01288249552
0.08912509381 0.04897788194 0.03090295433 0.06025596 0.01621810097
0.0977237221 0.05370317964 0.03548133892 0.06606934 0.02089296131
0.10715193052 0.05754399373 0.04073802778 0.0724436 0.02570395783
0.11748975549 0.06165950019 0.04677351413 0.07943282 0.03090295433
0.12882495517 0.06918309709 0.05495408739 0.08709636 0.03715352291
0.14454397707 0.07413102413 0.06025595861 0.09772372 0.04466835922
0.15488166189 0.08128305162 0.06760829754 0.10471285 0.05248074602
0.16595869074 0.08511380382 0.07244359601 0.11220185 0.05888436554
0.18197008586 0.09120108394 0.08128305162 0.12302688 0.06760829754
0.19498445998 0.0977237221 0.08912509381 0.13182567 0.0758577575
0.20892961309 0.10471285481 0.0954992586 0.14125375 0.08511380382
0.22387211386 0.10964781961 0.10232929923 0.15135612 0.0954992586
0.2398832919 0.11481536215 0.11220184543 0.16218101 0.10715193052
0.25118864315 0.12302687708 0.12302687708 0.16982437 0.11748975549
0.26302679919 0.12882495517 0.12882495517 0.17782794 0.12882495517
0.27542287033 0.13489628826 0.13803842646 0.18620871 0.14125375446
0.28840315031 0.14125375446 0.14454397707 0.19498446 0.15135612484
0.30199517204 0.14791083882 0.15135612484 0.20417379 0.16218100974
0.31622776602 0.15135612484 0.15848931925 0.21379621 0.16982436525
0.32359365693 0.15848931925 0.16982436525 0.21877616 0.18197008586
0.33884415614 0.16595869074 0.177827941 0.22908677 0.19498445998
0.34673685045 0.16982436525 0.18620871367 0.23442288 0.20892961309
0.36307805477 0.17378008287 0.19498445998 0.24547089 0.21877616239
0.38018939632 0.18197008586 0.1995262315 0.25703958 0.22908676528
0.38904514499 0.18620871367 0.20892961309 0.2630268 0.2398832919
0.39810717055 0.1905460718 0.21877616239 0.26915348 0.25118864315
0.4073802778 0.19498445998 0.22908676528 0.27542287 0.26302679919
0.41686938347 0.20417379447 0.23442288153 0.28183829 0.27542287033
0.08912509381 0.04897788194 0.03090295433 0.06025596 0.01584893192
0.07762471166 0.04466835922 0.02570395783 0.05248075 0.01288249552
0.06918309709 0.04073802778 0.02187761624 0.04677351 0.01
0.06165950019 0.03715352291 0.01862087137 0.04168694 0.00741310241
0.05623413252 0.03548133892 0.01548816619 0.03801894 0.00575439937
0.04897788194 0.03235936569 0.01288249552 0.03311311 0.0045708819
0.04073802778 0.02754228703 0.01 0.02754229 0.00309029543
0.03388441561 0.02344228815 0.00776247117 0.02290868 0.00204173794
0.02818382931 0.02089296131 0.00588843655 0.01905461 0.00134896288
0.02290867653 0.01819700859 0.00467735141 0.01548817 0.00091201084
0.01862087137 0.01479108388 0.00316227766 0.01258925 0.00052480746
0.01479108388 0.01202264435 0.00208929613 0.01 0.00029512092
0.01071519305 0.00933254301 0.00147910839 0.00724436 0.00015848932
0.0072443596 0.00691830971 0.00081283052 0.0047863 7.94328235E-05
0.00501187234 0.00501187234 0.00045708819 0.00331131 3.80189396E-05
0.00338844156 0.00371535229 0.00028183829 0.00223872 1.77827941E-05
0.00199526231 0.0026915348 0.00014454398 0.00131826 7.94328235E-06
0.00134896288 0.00169824365 6.45654229E-05 0.00089125 2.8840315E-06
0.00070794578 0.00123026877 3.38844156E-05 0.00046774 1.28824955E-06
0.00037153523 0.00074131024 1.41253754E-05 0.00024547 4.67735141E-07
0.00018620871 0.00040738028 4.89778819E-06 0.00012303 1.6218101E-07
8.51138038E-05 0.00023442288 1.81970086E-06 5.62341E-05 5.37031796E-08
3.4673685E-05 0.00012302688 6.30957344E-07 2.23872E-05 1.73780083E-08
1.14815362E-05 5.37031796E-05 1.73780083E-07 7.4131E-06 5.75439937E-09
3.4673685E-06 2.13796209E-05 4.07380278E-08 2.23872E-06 3.38844156E-09
7.2443596E-07 7.58577575E-06 9.77237221E-09 4.67735E-07 1.86208714E-09
1.41253754E-07 1.9498446E-06 3.54813389E-09 8.91251E-08 1.14815362E-09
1.99526231E-08 2.18776162E-07 1.28824955E-09 1.25893E-08 7.58577575E-10
3.71535229E-09 1.04712855E-08 6.02559586E-10 2.34423E-09 5.88843655E-10
1.20226443E-09 8.7096359E-10 4.46683592E-10 7.4131E-10 3.4673685E-10
9.33254301E-10 4.46683592E-10 2.51188643E-10 5.7544E-10 2.63026799E-10
 c) cálculo de F0
T1 T2 T3 T4 T5
0 0 0 0 0
6.01132217E-10 4.35481795E-10 2.23341796E-10 3.70655121E-10 1.73368425E-10
1.85767615E-09 5.23564274E-09 3.01279793E-10 1.17211441E-09 2.94421828E-10
9.97631157E-09 1.09388081E-07 6.44124776E-10 6.29462706E-09 3.79288788E-10
7.06268772E-08 9.749223E-07 1.77406695E-09 4.45625469E-08 5.74076811E-10
3.6221798E-07 3.79288788E-06 4.8861861E-09 2.33867571E-07 9.31043568E-10
1.73368425E-06 1.06898104E-05 2.03690139E-08 1.11936057E-06 1.69422078E-09
5.74076811E-06 2.68515898E-05 8.68900414E-08 3.70655121E-06 2.87719969E-09
1.73368425E-05 6.15134385E-05 3.15478672E-07 1.11936057E-05 8.68900414E-09
4.25569019E-05 0.00011721144 9.09850429E-07 2.81170663E-05 2.68515898E-08
9.31043568E-05 0.00020369014 2.4488941E-06 6.15134385E-05 8.10905049E-08
0.00018576761 0.00037065512 7.06268772E-06 0.00012273545 2.33867571E-07
0.00035397289 0.00061513439 1.69422078E-05 0.00023386757 6.44124776E-07
0.00067448144 0.00084912183 3.22827115E-05 0.00044562547 1.44201575E-06
0.00099763116 0.0013457674 7.22719885E-05 0.00065912837 3.97164117E-06
0.00169422078 0.00185767615 0.00014091915 0.00111936057 8.89139705E-06
0.00250593617 0.00250593617 0.00022854409 0.00165565561 1.90094698E-05
0.0036221798 0.00345915485 0.00040641526 0.00239315046 3.97164117E-05
0.00535759653 0.0046662715 0.00073955419 0.0036221798 7.92446596E-05
0.00739554194 0.00601132217 0.00104464807 0.005 0.00014756046
0.00931043568 0.00739554194 0.00158113883 0.00629462706 0.00026240373
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