The aim of this study was to characterize and quantify metabolic acidosis that was caused by init... more The aim of this study was to characterize and quantify metabolic acidosis that was caused by initial volume expansion during the reanimation of patients with severe sepsis and septic shock. A blood sample was drawn for physicochemical characterization of the patient's acid-base equilibrium both before and after volume expansion using 30 mL/kg 0.9% saline solution. The diagnosis and quantification of metabolic acidosis were based on the standard base excess (SBE). Eight patients with a mean age of 58 ± 13 years and mean APACHE II scores of 20 ± 4 were expanded using 2,000 ± 370 mL of 0.9% saline solution. Blood pH dropped from 7.404 ± 0.080 to 7.367 ± 0.086 (p=0.018), and PC O2 increased from 30 ± 5 to 32 ± 2 mmHg (p=0.215); SBE dropped from -4.4 ± 5.6 to -6.0 ± 5.7 mEq/L (p=0.039). The drop in SBE was associated with the acidifying power of two factors, namely, a significant increase in the strong ion gap (SIG) from 6.1 ± 3.4 to 7.7 ± 4.0 mEq/L (p = 0.134) and a non-significant ...
Jornal brasileiro de pneumologia : publicaça̋o oficial da Sociedade Brasileira de Pneumologia e Tisilogia, 2008
Silicosis, the most prevalent of the pneumoconioses, is caused by inhalation of crystalline silic... more Silicosis, the most prevalent of the pneumoconioses, is caused by inhalation of crystalline silica particles. Silica-exposed workers, with or without silicosis, are at increased risk for tuberculosis and nontuberculous mycobacteria-related diseases. The risk of a patient with silicosis developing tuberculosis is higher (2.8 to 39 times higher, depending on the severity of the silicosis) than that found for healthy controls. Various regimens for tuberculosis chemoprophylaxis in patients with silicosis have been studied, all of which present similar efficacy and overall risk reduction to about one half of that obtained with placebo. Long-term regimens have potential side effects (particularly hepatotoxicity). In addition, the use of such regimens can jeopardize adherence to treatment. The current guidelines recommend that tuberculin skin tests be performed, and, if positive, that chemoprophylaxis be instituted. There are several possible regimens, varying in terms of the drugs prescri...
The International journal of periodontics & restorative dentistry
Twenty-four teeth were extracted en bloc with the marginal periodontal structures and processed f... more Twenty-four teeth were extracted en bloc with the marginal periodontal structures and processed for histologic purposes. Measurements were taken from the top of the gingival margin to the apical limit of the junctional epithelium (GM-JE), from the apical limit of the junctional epithelium to the top of the alveolar bone crest (JE-AB), and from the top of the gingival margin to the top of the alveolar bone crest (GM-AB). Data were histometrically analyzed. This first clinical human study demonstrated mean measurements of 1.58 ± 0.41 mm for GM-JE, 1.18 ± 0.42 mm for JE-AB, and 2.75 ± 0.59 mm for GM-AB.
A variety of gingival enlargements can occur in children, but they are rarely observed in the inf... more A variety of gingival enlargements can occur in children, but they are rarely observed in the infant. The aim of this study is to present a case of a ten-month old male patient, with an anterior mandibular hamartoma associated to natal teeth, and to describe the clinical and histological characteristics of this anomaly and the treatment performed.
Jornal brasileiro de pneumologia : publicaça̋o oficial da Sociedade Brasileira de Pneumologia e Tisilogia, 2008
Pulmonary veno-occlusive disease (PVOD) is a rare cause of pulmonary hypertension. Surgical biops... more Pulmonary veno-occlusive disease (PVOD) is a rare cause of pulmonary hypertension. Surgical biopsy was usually required for diagnostic confirmation. However, the morbidity, mortality and limited benefit of this procedure have generated discussion regarding noninvasive diagnostic techniques. We present the case of a female patient with progressive dyspnea, hypoxemia and pulmonary hypertension, the last diagnosed via catheterization. Computed tomography revealed septal thickening and diffuse micronodules. Bronchoalveolar lavage revealed occult alveolar hemorrhage. Treatment with an endothelin antagonist was started, resulting in symptomatic and functional improvement. Occult alveolar hemorrhage differentiates PVOD from idiopathic pulmonary hypertension. We believe that this finding, in combination with characteristic tomographic findings, is sufficient to establish a diagnosis of PVOD.
A deficiência de alfa-1 antitripsina é um distúrbio genético de descoberta recente e que ocorre c... more A deficiência de alfa-1 antitripsina é um distúrbio genético de descoberta recente e que ocorre com freqüência comparável à da fibrose cística. Resulta de diferentes mutações no gene SERPINA1 e tem diversas implicações clínicas. A alfa-1 antitripsina é produzida principalmente no fígado e atua como uma antiprotease. Tem como principal função inativar a elastase neutrofílica, impedindo a ocorrência de dano tecidual. A mutação mais freqüentemente relacionada à doença clínica é o alelo Z, que determina polimerização e acúmulo dentro dos hepatócitos. O acúmulo e a conseqüente redução dos níveis séricos de alfa-1 antitripsina determinam, respectivamente, doença hepática e pulmonar, sendo que esta se manifesta principalmente sob a forma de enfisema de aparecimento precoce, habitualmente com predomínio basal. O diagnóstico envolve a detecção de níveis séricos reduzidos de alfa-1 antitripsina e a confirmação fenotípica. Além do tratamento usual para doença pulmonar obstrutiva crônica, existe atualmente uma terapia específica com infusão de concentrados de alfa-1 antitripsina. Essa terapia de reposição, aparentemente segura, ainda não teve a eficácia clínica definitivamente comprovada, e o custo-efetividade também é um tema controverso e ainda pouco abordado. Apesar da sua importância, não existem dados epidemiológicos brasileiros a respeito da prevalência da doença ou da freqüência de ocorrência dos alelos deficientes. O subdiagnóstico também tem sido uma importante limitação tanto para o estudo da doença quanto para o tratamento adequado dos pacientes. Espera-se que a criação do Registro Internacional de Alfa-1 venha a resolver essas e outras importantes questões.
ABSTRACT The isolation of Antarctica from South America during the Oligocene (~35 mya) formed the... more ABSTRACT The isolation of Antarctica from South America during the Oligocene (~35 mya) formed the Southern Ocean, generated the northward flow of the Atlantic Antarctic Bottom Water, and numerous unique geological and oceanic processes (e.g. an active spreading centre in the Bransfield Strait, ridge trench collision, gas hydrates on modern sediments, and complex circulation) along the northern end of the Antarctic Peninsula in particular (Barker and Burrell 1982; Pearse et al. 2001; Barker and Thomas 2004; Thomson 2004; Turner et al. 2009).
... Carlos Eduardo Galvão Barboza1, Carlos Viana Poyares Jardim2, André Luís Dressler Hovnanian3,... more ... Carlos Eduardo Galvão Barboza1, Carlos Viana Poyares Jardim2, André Luís Dressler Hovnanian3, Bruno Arantes Dias3, Rogério Souza4 ... Chest. 2002;122(3):1096-8. 12. Palmer SM, Robinson LJ, Wang A, Gossage JR, Bashore T, Tapson VF. ...
A number of evidences show the influence of the growth of injured nerve fibers in peripheral nerv... more A number of evidences show the influence of the growth of injured nerve fibers in peripheral nervous system as well as potential implant stem cells (SCs). The SCs implementation in the clinical field is promising and the understanding of proliferation and differentiation is essential. This study aimed to evaluate the plasticity of mesenchymal SCs from bone marrow of mice in the presence of culture medium conditioned with facial nerve explants and fibroblast growth factor-2 (FGF-2). The growth and morphology were assessed for over 72 hours. Quantitative phenotypic analysis was taken from the immunocytochemistry for glial fibrillary acidic protein (GFAP), protein OX-42 (OX-42), protein associated with microtubule MAP-2 (MAP-2), protein β-tubulin III (β-tubulin III), neuronal nuclear protein (NeuN), and neurofilament 200 (NF-200). Cells cultured with conditioned medium alone or combined with FGF-2 showed morphological features apparently similar at certain times to neurons and glia and...
A silicose, a mais prevalente das pneumoconioses, é provocada pela inalação de partículas de síli... more A silicose, a mais prevalente das pneumoconioses, é provocada pela inalação de partículas de sílica cristalina. Indivíduos expostos à sílica, com ou sem silicose, apresentam risco aumentado de tuberculose e de micobacterioses não-tuberculosas. O risco de silicóticos desenvolverem tuberculose em relação a controles sadios varia de 2,8 a 39 vezes, em conformidade com a gravidade da doença de base. Têm sido estudados diferentes esquemas de quimioprofilaxia para tuberculose em silicóticos, todos com eficácia semelhante e com redução final de risco para cerca da metade em relação ao uso de placebo. São, no entanto, esquemas de longa duração, o que, acrescido dos possíveis efeitos colaterais (particularmente hepatotoxicidade), podem prejudicar a aderência. As diretrizes atuais recomendam a realização de prova tuberculínica e, se positiva, a instituição de quimioprofilaxia. São vários os esquemas possíveis, tanto em termos de drogas quanto de duração. Nossa recomendação é de que se use iso...
The International Journal of Periodontics and Restorative Dentistry, 2014
This study analyzed the length of the root trunk (RT) as well as the width and depth of the prefu... more This study analyzed the length of the root trunk (RT) as well as the width and depth of the prefurcation area (PFA) of human mandibular first molars. Ninety-six human mandibular first molars that were extracted after being observed to be in poor condition both clinically and radiographically were used in this study. A contour-measuring instrument was used, and profiles of the buccal and lingual root surfaces of these teeth were traced on a millimeter-scaled paper. The profiles were recorded from the cementoenamel junction, millimeter by millimeter, up to the entrance of the furcation of each tooth. The width and depth of the PFA as well as the length of the RT were studied. After statistical analysis it was concluded that the mean widths of the PFA on the buccal and lingual surfaces were 3.68 ± 0.66 and 3.48 ± 0.51 mm, respectively; mean depths of the PFA on the buccal and lingual surfaces were 0.89 ± 0.42 and 0.82 ± 0.28 mm, respectively; and mean lengths of the RT on the buccal and lingual surfaces were 3.07 ± 0.82 and 3.54 ± 0.73 mm. Of the samples, the lengths of the RT and the PFA were coincident in 39.58% on the buccal surface and in 46.87% on the lingual surface. There is a concavity (PFA) in the RT region of all mandibular first molars, showing larger width and depth on the buccal surface. The RT was longer on the lingual surface in comparison with the buccal surface.
Page 1. Oecol. Aust., 15(1): 86-110, 2011 Oecologia Australis 15(1): 86-110, Março 2011 doi:10.42... more Page 1. Oecol. Aust., 15(1): 86-110, 2011 Oecologia Australis 15(1): 86-110, Março 2011 doi:10.4257/oeco.2011.1501.08 ECHINODERMS AS CLUES TO ANTARCTIC ~ SOUTH AMERICAN CONNECTIVITY Carlos Alberto ...
Peripheral nerve trauma results in functional loss in the innervated organ, and recovery without ... more Peripheral nerve trauma results in functional loss in the innervated organ, and recovery without surgical intervention is rare. Many surgical techniques can be used for repair in experimental models. The authors investigated the source and delivery method of stem cells in experimental outcomes, seeking to clarify whether stem cells must be differentiated in the injured facial nerve and improve the regenerative process. The following key terms were used: nervous regeneration, nerve regeneration, facial nerve regeneration, stem cells, embryonic stem cells, fetal stem cells, adult stem cells, facial nerve, facial nerve trauma, and facial nerve traumatism. The search was restricted to experimental studies that applied stem cell therapy and tissue engineering for nerve repair. Eight studies meeting the inclusion criteria were reviewed. Different sources of stem and precursor cells were explored (bone marrow mesenchymal stem cells, adipose-derived stem cells, dental pulp cells, and neural stem cells) for their potential application in the scenario of facial nerve injuries. Different material conduits (vases, collagen, and polyglycolic acid) were used as bridges. Immunochemistry and electrophysiology are the principal methods for analyzing regenerative effects. Although recent studies have shown that stem cells can act as a promising bridge for nerve repair, considerable optimization of these therapies will be required for their potential to be realized in a clinical setting. Based on these studies, the use of stem cells derived from different sources presents promising results related to facial nerve regeneration and produces effective functional results. The use of tubes also optimizes nerve repair, thus promoting greater myelination and axonal growth of peripheral nerves.
The aim of this study was to characterize and quantify metabolic acidosis that was caused by init... more The aim of this study was to characterize and quantify metabolic acidosis that was caused by initial volume expansion during the reanimation of patients with severe sepsis and septic shock. A blood sample was drawn for physicochemical characterization of the patient's acid-base equilibrium both before and after volume expansion using 30 mL/kg 0.9% saline solution. The diagnosis and quantification of metabolic acidosis were based on the standard base excess (SBE). Eight patients with a mean age of 58 ± 13 years and mean APACHE II scores of 20 ± 4 were expanded using 2,000 ± 370 mL of 0.9% saline solution. Blood pH dropped from 7.404 ± 0.080 to 7.367 ± 0.086 (p=0.018), and PC O2 increased from 30 ± 5 to 32 ± 2 mmHg (p=0.215); SBE dropped from -4.4 ± 5.6 to -6.0 ± 5.7 mEq/L (p=0.039). The drop in SBE was associated with the acidifying power of two factors, namely, a significant increase in the strong ion gap (SIG) from 6.1 ± 3.4 to 7.7 ± 4.0 mEq/L (p = 0.134) and a non-significant ...
Jornal brasileiro de pneumologia : publicaça̋o oficial da Sociedade Brasileira de Pneumologia e Tisilogia, 2008
Silicosis, the most prevalent of the pneumoconioses, is caused by inhalation of crystalline silic... more Silicosis, the most prevalent of the pneumoconioses, is caused by inhalation of crystalline silica particles. Silica-exposed workers, with or without silicosis, are at increased risk for tuberculosis and nontuberculous mycobacteria-related diseases. The risk of a patient with silicosis developing tuberculosis is higher (2.8 to 39 times higher, depending on the severity of the silicosis) than that found for healthy controls. Various regimens for tuberculosis chemoprophylaxis in patients with silicosis have been studied, all of which present similar efficacy and overall risk reduction to about one half of that obtained with placebo. Long-term regimens have potential side effects (particularly hepatotoxicity). In addition, the use of such regimens can jeopardize adherence to treatment. The current guidelines recommend that tuberculin skin tests be performed, and, if positive, that chemoprophylaxis be instituted. There are several possible regimens, varying in terms of the drugs prescri...
The International journal of periodontics & restorative dentistry
Twenty-four teeth were extracted en bloc with the marginal periodontal structures and processed f... more Twenty-four teeth were extracted en bloc with the marginal periodontal structures and processed for histologic purposes. Measurements were taken from the top of the gingival margin to the apical limit of the junctional epithelium (GM-JE), from the apical limit of the junctional epithelium to the top of the alveolar bone crest (JE-AB), and from the top of the gingival margin to the top of the alveolar bone crest (GM-AB). Data were histometrically analyzed. This first clinical human study demonstrated mean measurements of 1.58 ± 0.41 mm for GM-JE, 1.18 ± 0.42 mm for JE-AB, and 2.75 ± 0.59 mm for GM-AB.
A variety of gingival enlargements can occur in children, but they are rarely observed in the inf... more A variety of gingival enlargements can occur in children, but they are rarely observed in the infant. The aim of this study is to present a case of a ten-month old male patient, with an anterior mandibular hamartoma associated to natal teeth, and to describe the clinical and histological characteristics of this anomaly and the treatment performed.
Jornal brasileiro de pneumologia : publicaça̋o oficial da Sociedade Brasileira de Pneumologia e Tisilogia, 2008
Pulmonary veno-occlusive disease (PVOD) is a rare cause of pulmonary hypertension. Surgical biops... more Pulmonary veno-occlusive disease (PVOD) is a rare cause of pulmonary hypertension. Surgical biopsy was usually required for diagnostic confirmation. However, the morbidity, mortality and limited benefit of this procedure have generated discussion regarding noninvasive diagnostic techniques. We present the case of a female patient with progressive dyspnea, hypoxemia and pulmonary hypertension, the last diagnosed via catheterization. Computed tomography revealed septal thickening and diffuse micronodules. Bronchoalveolar lavage revealed occult alveolar hemorrhage. Treatment with an endothelin antagonist was started, resulting in symptomatic and functional improvement. Occult alveolar hemorrhage differentiates PVOD from idiopathic pulmonary hypertension. We believe that this finding, in combination with characteristic tomographic findings, is sufficient to establish a diagnosis of PVOD.
A deficiência de alfa-1 antitripsina é um distúrbio genético de descoberta recente e que ocorre c... more A deficiência de alfa-1 antitripsina é um distúrbio genético de descoberta recente e que ocorre com freqüência comparável à da fibrose cística. Resulta de diferentes mutações no gene SERPINA1 e tem diversas implicações clínicas. A alfa-1 antitripsina é produzida principalmente no fígado e atua como uma antiprotease. Tem como principal função inativar a elastase neutrofílica, impedindo a ocorrência de dano tecidual. A mutação mais freqüentemente relacionada à doença clínica é o alelo Z, que determina polimerização e acúmulo dentro dos hepatócitos. O acúmulo e a conseqüente redução dos níveis séricos de alfa-1 antitripsina determinam, respectivamente, doença hepática e pulmonar, sendo que esta se manifesta principalmente sob a forma de enfisema de aparecimento precoce, habitualmente com predomínio basal. O diagnóstico envolve a detecção de níveis séricos reduzidos de alfa-1 antitripsina e a confirmação fenotípica. Além do tratamento usual para doença pulmonar obstrutiva crônica, existe atualmente uma terapia específica com infusão de concentrados de alfa-1 antitripsina. Essa terapia de reposição, aparentemente segura, ainda não teve a eficácia clínica definitivamente comprovada, e o custo-efetividade também é um tema controverso e ainda pouco abordado. Apesar da sua importância, não existem dados epidemiológicos brasileiros a respeito da prevalência da doença ou da freqüência de ocorrência dos alelos deficientes. O subdiagnóstico também tem sido uma importante limitação tanto para o estudo da doença quanto para o tratamento adequado dos pacientes. Espera-se que a criação do Registro Internacional de Alfa-1 venha a resolver essas e outras importantes questões.
ABSTRACT The isolation of Antarctica from South America during the Oligocene (~35 mya) formed the... more ABSTRACT The isolation of Antarctica from South America during the Oligocene (~35 mya) formed the Southern Ocean, generated the northward flow of the Atlantic Antarctic Bottom Water, and numerous unique geological and oceanic processes (e.g. an active spreading centre in the Bransfield Strait, ridge trench collision, gas hydrates on modern sediments, and complex circulation) along the northern end of the Antarctic Peninsula in particular (Barker and Burrell 1982; Pearse et al. 2001; Barker and Thomas 2004; Thomson 2004; Turner et al. 2009).
... Carlos Eduardo Galvão Barboza1, Carlos Viana Poyares Jardim2, André Luís Dressler Hovnanian3,... more ... Carlos Eduardo Galvão Barboza1, Carlos Viana Poyares Jardim2, André Luís Dressler Hovnanian3, Bruno Arantes Dias3, Rogério Souza4 ... Chest. 2002;122(3):1096-8. 12. Palmer SM, Robinson LJ, Wang A, Gossage JR, Bashore T, Tapson VF. ...
A number of evidences show the influence of the growth of injured nerve fibers in peripheral nerv... more A number of evidences show the influence of the growth of injured nerve fibers in peripheral nervous system as well as potential implant stem cells (SCs). The SCs implementation in the clinical field is promising and the understanding of proliferation and differentiation is essential. This study aimed to evaluate the plasticity of mesenchymal SCs from bone marrow of mice in the presence of culture medium conditioned with facial nerve explants and fibroblast growth factor-2 (FGF-2). The growth and morphology were assessed for over 72 hours. Quantitative phenotypic analysis was taken from the immunocytochemistry for glial fibrillary acidic protein (GFAP), protein OX-42 (OX-42), protein associated with microtubule MAP-2 (MAP-2), protein β-tubulin III (β-tubulin III), neuronal nuclear protein (NeuN), and neurofilament 200 (NF-200). Cells cultured with conditioned medium alone or combined with FGF-2 showed morphological features apparently similar at certain times to neurons and glia and...
A silicose, a mais prevalente das pneumoconioses, é provocada pela inalação de partículas de síli... more A silicose, a mais prevalente das pneumoconioses, é provocada pela inalação de partículas de sílica cristalina. Indivíduos expostos à sílica, com ou sem silicose, apresentam risco aumentado de tuberculose e de micobacterioses não-tuberculosas. O risco de silicóticos desenvolverem tuberculose em relação a controles sadios varia de 2,8 a 39 vezes, em conformidade com a gravidade da doença de base. Têm sido estudados diferentes esquemas de quimioprofilaxia para tuberculose em silicóticos, todos com eficácia semelhante e com redução final de risco para cerca da metade em relação ao uso de placebo. São, no entanto, esquemas de longa duração, o que, acrescido dos possíveis efeitos colaterais (particularmente hepatotoxicidade), podem prejudicar a aderência. As diretrizes atuais recomendam a realização de prova tuberculínica e, se positiva, a instituição de quimioprofilaxia. São vários os esquemas possíveis, tanto em termos de drogas quanto de duração. Nossa recomendação é de que se use iso...
The International Journal of Periodontics and Restorative Dentistry, 2014
This study analyzed the length of the root trunk (RT) as well as the width and depth of the prefu... more This study analyzed the length of the root trunk (RT) as well as the width and depth of the prefurcation area (PFA) of human mandibular first molars. Ninety-six human mandibular first molars that were extracted after being observed to be in poor condition both clinically and radiographically were used in this study. A contour-measuring instrument was used, and profiles of the buccal and lingual root surfaces of these teeth were traced on a millimeter-scaled paper. The profiles were recorded from the cementoenamel junction, millimeter by millimeter, up to the entrance of the furcation of each tooth. The width and depth of the PFA as well as the length of the RT were studied. After statistical analysis it was concluded that the mean widths of the PFA on the buccal and lingual surfaces were 3.68 ± 0.66 and 3.48 ± 0.51 mm, respectively; mean depths of the PFA on the buccal and lingual surfaces were 0.89 ± 0.42 and 0.82 ± 0.28 mm, respectively; and mean lengths of the RT on the buccal and lingual surfaces were 3.07 ± 0.82 and 3.54 ± 0.73 mm. Of the samples, the lengths of the RT and the PFA were coincident in 39.58% on the buccal surface and in 46.87% on the lingual surface. There is a concavity (PFA) in the RT region of all mandibular first molars, showing larger width and depth on the buccal surface. The RT was longer on the lingual surface in comparison with the buccal surface.
Page 1. Oecol. Aust., 15(1): 86-110, 2011 Oecologia Australis 15(1): 86-110, Março 2011 doi:10.42... more Page 1. Oecol. Aust., 15(1): 86-110, 2011 Oecologia Australis 15(1): 86-110, Março 2011 doi:10.4257/oeco.2011.1501.08 ECHINODERMS AS CLUES TO ANTARCTIC ~ SOUTH AMERICAN CONNECTIVITY Carlos Alberto ...
Peripheral nerve trauma results in functional loss in the innervated organ, and recovery without ... more Peripheral nerve trauma results in functional loss in the innervated organ, and recovery without surgical intervention is rare. Many surgical techniques can be used for repair in experimental models. The authors investigated the source and delivery method of stem cells in experimental outcomes, seeking to clarify whether stem cells must be differentiated in the injured facial nerve and improve the regenerative process. The following key terms were used: nervous regeneration, nerve regeneration, facial nerve regeneration, stem cells, embryonic stem cells, fetal stem cells, adult stem cells, facial nerve, facial nerve trauma, and facial nerve traumatism. The search was restricted to experimental studies that applied stem cell therapy and tissue engineering for nerve repair. Eight studies meeting the inclusion criteria were reviewed. Different sources of stem and precursor cells were explored (bone marrow mesenchymal stem cells, adipose-derived stem cells, dental pulp cells, and neural stem cells) for their potential application in the scenario of facial nerve injuries. Different material conduits (vases, collagen, and polyglycolic acid) were used as bridges. Immunochemistry and electrophysiology are the principal methods for analyzing regenerative effects. Although recent studies have shown that stem cells can act as a promising bridge for nerve repair, considerable optimization of these therapies will be required for their potential to be realized in a clinical setting. Based on these studies, the use of stem cells derived from different sources presents promising results related to facial nerve regeneration and produces effective functional results. The use of tubes also optimizes nerve repair, thus promoting greater myelination and axonal growth of peripheral nerves.
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