Background: EndoActivator, a sonically-driven canal irrigation system (Dentsply Tulsa Dental Spec... more Background: EndoActivator, a sonically-driven canal irrigation system (Dentsply Tulsa Dental Specialties, Tulsa, OK), has been developed for activating root canal irrigants, and has recently been released onto the market. Purpose: To obtain an initial understanding of bubbles generation of electrical EndoActivator tips during activation of the irrigant in a transparent root canal model and a modified extracted tooth. Methods: A modified extracted tooth and a straight glass model were filled with a solution containing 17% EDTA or 3% NaOCl. A medium activator tip 22-mm polymer noncutting #25, 0.04 file driven by an electrical sonic hand-piece at 190 Hz (highest level) induced pressure waves that produced macro- and micro-bubbles. The physical mechanisms involved were visualized using a Miro 320S high-speed imaging system (Phantom, Wayne, NJ, USA) with high temporal and spatial resolutions. The imaging system acquired images at 25,000 frames per second with 320×x240 pixels per image, a...
This article was migrated. The article was not marked as recommended. Purpose: Research-informed ... more This article was migrated. The article was not marked as recommended. Purpose: Research-informed teaching is a crucial component of university education, and exposure to lectures by world scientists is one way to motivate undergraduate students to develop the future of dentistry. This study aimed to examine dental students' perceptions of international videoconferencing lectures on basic/clinical research, as well as Japanese dental students' perceptions of the use of videoconferencing lectures in large classes. Methods: We administered a questionnaire survey to senior undergraduate students in dental schools that participated in videoconferencing lectures in 2015/2016 in Japan (n=92) and Indonesia (n=175) and analyzed the data quantitatively and qualitatively. Results: Half of the Japanese and Indonesian students reported being motivated by the presence of foreign dental students in the videoconferencing lectures although there were significant differences between the Japan...
Background: Tissue engineering using Stem cell from Human Exfoliated Deciduous Teeth (SHED) and a... more Background: Tissue engineering using Stem cell from Human Exfoliated Deciduous Teeth (SHED) and a natural biomaterials biomaterial scaffold has become a promising therapy for the alveolar bone defect. The aim of this study was to analyze the Osteoprotegerin (OPG) and Receptor Activator of NF-Κb ligand (RANKL) expression after the application of Hydroxyapatite scaffold and SHED. Methods: A laboratory experimental research with a post test-only control group. 14 male Wistar rats weighing from 260 to 280 g were used as the animal study. The animals were randomly assigned to an experimental group Hydroxyapatite scaffold (group I) and Hydroxyapatite scaffold combined with SHED (group II). The alveolar bone defect in the animal study model was affected by extracting anterior mandible teeth. Immunostaining was performed after 8 weeks in order to facilitate the examination of OPG and RANKL expression. Data were analyzed by independent t test. The correlation between OPG and RANKL expression...
tujuan dari pengobatan regeneratif jaringan pulpa adalah untuk meregenerasi jaringan normal pada ... more tujuan dari pengobatan regeneratif jaringan pulpa adalah untuk meregenerasi jaringan normal pada daerah pertemuan antara pulpa dan dentin. jaringan ini merupakan jaringan yang meregulasi pembentukan dentin tersier. jaringan pulpa terdirivdari sel progenitor yang berpoliferasic dan berdiferensiasi menjadi odontoblas. odontoblas yang rusak dapat diganti oleh cell yang menyerupai odontoblas yang berassal dari sel fibroblast pulpa. dalam proses ini beberapa pembentukan dentin reparatif. exegenous FGF-2 akan berikatan dengan reseptornya pada permukaan sel fibroblas yang akan akan menginduksi cell signaling pada tulisan ini akan dibahas mengenai peran FGF-2 dalam proliferasi sel fibroblast yang akan menginduksi cell signaling. pada tulisan ini akan dibahas mengenai peran FGF-2 dalam proliferasi sel fibroblas pulpa. peran FGF-2 sangat pending pada proses pembentukan dentin reparatif. FGF-2 meningkatkan proliferasi dan diferensiasi sel fibroblas pulpa. maj. ked.gi; desember 2008 ;15(@): 191...
Background: Alveolar bone defects in children still have a high incidence. Conventional bone graf... more Background: Alveolar bone defects in children still have a high incidence. Conventional bone graft technique that has been used as a defect therapy is still not effective, so new techniques with tissue engineering approach are needed. Bone Morphogenetic Protein 4 (BMP4) as one of the indicators of osteogenic differentiation has not been widely studied, especially in the transplantation with combination of Stem Cells from Human Exfoliated Deciduous (SHED) and carbonate apatite. Aim and Objectives: This research aimed to determine the expression of BMP4 after SHED and carbonate apatite transplantation on Rattus norvegicus. Material and Methods: The combinations of SHED and carbonate apatite were transplanted on alveolar bone defects of 4 rats (Rattus norvegicus) as the treatment groups and another 4 rats were transplanted with carbonate apatite as the control groups. After 21 days, staining with Hematoxylin Eosin (HE) and Immunohistochemistry (IHC) BMP4 was performed. Results: BMP4 ex...
Stem cells can give a new hope to accelerate wound healing process and can also be used for the t... more Stem cells can give a new hope to accelerate wound healing process and can also be used for the treatment of various diseases, including diseases on periodontal tissues (periodontitis) since there is still not any materials known to be able to attach wobbly teeth perfectly. Adult stem cells derived from bone marrow have widely been used nowadays. However, the number of stem cells is limited, consequently, growth factors are necessary to increase the proliferation of stem cells. Unfortunately, growth factors that have been used are still expensive and difficult to obtain. Therefore, an alternative to the use of growth factors from natural materials that can potentially accelerate wound healing process is necessary to be developed. Purpose: Golden Sea Cucumbers (Stichopus hermanii) is rich in both growth factors that can repair damaged cells and protein that reaches up to 82% of all components of the golden sea cucumbers, 80% of which is collagen. Therefore, this research aims to prov...
Periodontal regeneration, especially regeneration of alveolar bone, remains difficult to achieve ... more Periodontal regeneration, especially regeneration of alveolar bone, remains difficult to achieve with the result that alternative therapies for achieving periodontal regeneration are still lacking. Tissue engineering using stem cells and biomaterial scaffold represents an alternative for solving this problem. Stem cells from human exfoliated deciduous teeth (SHED) are one source of adult stem cells which can proliferate and differentiate into many types of specific cells. Hydroxyapatite (HA) constitutes a bioactive scaffold possessing osseointegration, osteoconduction and osteogenesis properties when used as bone graft. The aim of this study was to analyze osteocalcin (OCN) and osteopontin (OPN) expression after the application of a combination of HA and SHED to alveolar bone defects. Eight experimental animals were divided into two groups. The first group was transplanted with HA only, while the second was transplanted with HA/SHED. The expression of OCN and OPN was examined 21 day...
The pulp chamber is mostly infected by anaerob gram negative bacteria and one of them is Porphyro... more The pulp chamber is mostly infected by anaerob gram negative bacteria and one of them is Porphyromonas gingivalis. The cell wall ofthis bacteria containing Lipopolysaccharide (LPS). LPS can penetrate to the periradicular tissue, act as endotoxin in host and causeperiradicular inflammation that leading to bone destruction. Bone destruction occur is due to the inflammation process that mediatedby immune system. This inflammation is mediated by immune system that can be found the cytokin pro-inflammatory infiltrated in thecell has an important role in bone destruction is known as osteoclast. Osteoclasts are multinucleated, terminally differentiated cellswhich play an essential role in bone resorption. Binding RANK with RANKL are increasing during the osteoclastogenesis process. Themain purpose of this study was to determine the increasing number of osteoclast in periapical tissue that induced by LPS. This studyused laboratory experimental with the post test only control group design. A...
Stem cells gave a new way to accelerate healing progress because the stem cells can be utilized f... more Stem cells gave a new way to accelerate healing progress because the stem cells can be utilized for the treatment of various degenerative diseases including periodontitis. The number of stem cells is limited; therefore, a growth factor is needed to increase stem cell proliferation. Growth factor that has been used is still expensive and difficult to obtain so it is necessary to develop an alternative use of growth factors from natural materials that potentially accelerate wound healing. Mangosteen (Gracinia mangostana L) contains xanthones which are flavonoid compounds that can activate kinase protein, as growth factor stem cells. To discover the potential effect of mangosteen skin extract (Gracinia mangostana L) as growth factor of stem cells. Proliferative ability test of mangosteen skin (Gracinia mangostana L) as a growth factor for mesenchymal stem cell (MSC) using MTT assay with FGF comparator. Test of osteogenic differentiation using Alizarin Red coloring. Viability cells in M...
Background. Chlorhexidine gluconate is one of endodontic irrigants that has excellence capability... more Background. Chlorhexidine gluconate is one of endodontic irrigants that has excellence capability to penetrate into dentin tubules and kill the pathogenic bacteria there. On the other hand, chlorhexidine gluconate has side effects to cause allergic reactions of the tissue and discoloration of the teeth. Xanthone from mangosteen pericarp can be considered as a natural alternative irrigant that usually has a good tolerance to the body. Purpose. The aim of this study compared the cytotoxicity between 0,78% xanthone from mangosteen pericarp and 0,2% chlorhexidine gluconate toward BHK-21 fibroblast cells. Methods. This study used experimental post-test only control group design. Xanthone from mangosteen pericarp preliminary cytotoxicity tested in various concentrations. Xanthone from mangosteen pericarp classified as a non-toxic concentration at 0,78%. Cytotoxicity of 0,78% xanthone from mangosteen pericarp compare with cytotoxicity of 0,2% chlorhexidine gluconate using MTT assay method...
Background: Stem cells from human exfoliated deciduous teeth (SHED) seeded in carbonate apatite s... more Background: Stem cells from human exfoliated deciduous teeth (SHED) seeded in carbonate apatite scaffold (CAS) may have multiple functions that could be used to regenerate the alveolar bone defects. The purpose of this study is to examine the ability of SHED and CAS in alveolar bone defects using an immunohistochemical analysis. Methods: ten three-month-old healthy male Wistar rats (R. novergicus) that weighed between 150–250 grams (g) were used as animal models. A simple blind random sampling method was used to select the sample that was assigned to the study group for CAS and SHED seeded in CAS (n=5). The animal study model of the alveolar bone was established by extracting the anterior mandible teeth. Rodent anesthesia was applied to relieve the pain during the procedure for all test animals. Immunohistochemistry was performed after seven days to facilitate the examination of the receptor activator of NF-κβ ligand (RANKL), osteoprotegrin (OPG), transforming growth factor-β (TGF-β...
Background: Enterococcus faecalis is one of the most common bacteria in root canal after endodont... more Background: Enterococcus faecalis is one of the most common bacteria in root canal after endodontic treatment. This species is found in about 77% of the cases that resistant to treatment. It is necessary to develop an alternative for intracanal dressing, one of the potential substance is natural ingredients. The stem of ambonese banana (Musa paradisiaca var. sapientum) extract contains active substances such as tannin, flavonoid, alkaloid, saponin, and antraquinone, which have been known for their antibacterial potency. Purpose: The aim of this study was to determine the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of ambonese banana stem (Musa paradisiaca var. sapientum) extract against Enterococcus faecalis. Method: This research was a laboratory experimental study. Ambonese banana stem extract was made by maceration method with ethanol 96% and certain dilution was performed to obtain various concentration. Value of MIC and MBC of ambonese b...
Background: Cleanliness of cavity is considered important for a restoration. Smear layer formed a... more Background: Cleanliness of cavity is considered important for a restoration. Smear layer formed after cavity preparation should be removed in order not to disrupt the bond adhesion between restorative materials and dental cavities. Saponins contained in mangosteen pericarp (Garcinia mangostana L.) have surfactant properties that can eliminate the smear layer assessed. 6% citric acid is a chelating agent which can eliminate the inorganic particles of the smear layer. Until now, the research on the differences of 0,78% saponin from mangosteen pericarp extract and 6% citric acid for cleanliness of cavity has never been done. Purpose: To see the differences between 0,78% saponin from mangosteen pericarp extract and 6% citric acid as cavity cleanser. Method: Eighteen human teeth with complete crown, no caries, and no fractures were randomized in 3 groups (n≥6), in this experiment use (n=6). The cavity was prepared using wheels bur for hand use instrument. After instrumentation, each cav...
Background:Enterococcus faecalis (E. faecalis) is a microorganism that is commonly found in endod... more Background:Enterococcus faecalis (E. faecalis) is a microorganism that is commonly found in endodontic failure treatment, this due to several characteristics of E.faecalis which has the capabillity to living in environments with high salt levels, high temperature, and pH broad spectrum. Bacteria in biofilms form is one of the adaptive process that allows bacteria to survive in an environment with low nutrients in the root canals. Bacteria in biofilms form have different characteristics from planktonic form, resistance to phagocytic cells and drugs, which can effect to persistent infection. Mangosteen (Garcinia mangostana) has many benefits, especially on the pericarp of the fruit contains alkaloids, tannins, phenolics, flavonoids, and triterpenoids. Flavonoids are the largest group of phenolic compounds that have a nature effectively inhibit the growth of viruses, bacteria, and fungi. Purpose:Purpose of this study wasto find out the role of the antibiofilm of the flavonoid in garcin...
Background : Dentinal hypersensitivity (DH) is a painful clinical condition and is characterized ... more Background : Dentinal hypersensitivity (DH) is a painful clinical condition and is characterized by pain arising from exposed dentin in response to various stimuli. Various treatment modalities are available to treat dentinal hypersensitivity which include at-home and in-office treatment. At home treatment generally consists of a variety of dentrifices containing different constituents like strontium chloride and bioactive glass (Novamin). These agents cause occlusion of dentinal tubules thereby reducing hypersensitivity.Purpose: This study was to evaluate the effects of different desensitizing dentifrices on dentinal tubule occlusion by scanning electron microscopy (SEM) of strontium chloride and bioactive glass (Novamin) pastes in the treatment of dentinehypersensitivity (DH) Methods: sixteen extractedspecimens from bovine incisors teeth with randomized into 2 groups (n=8). The crowns were removed from the root and the crown were sectioned longitudinally into two parts (in a me...
Back Ground: Adherence of Stretococcus mutans biofilm (S. mutans) to the surface of dental resto... more Back Ground: Adherence of Stretococcus mutans biofilm (S. mutans) to the surface of dental restorative materials is considered an important step in the development of secondary caries and periodontal disease. There are two type of nanofil composite: universal restorative and flowable restorative. That have different characteristic to induce S.mutans biofilm adherent in it surface. Purpose: The aim of this study was to compare the adherence of S. mutans biofilm to two types of nanofil restorative materials, flowable restorative and universal restorative.. Materials and Methode: 32 disc-shaped specimens (∅ = 5.0 mm / thickness = 2.0 mm) of two types composite were divided to 4 groups (n = 8): group 1; universal restorative were immersed in pH cycling solution for 14 days ,group 2; Universal restorative were immersed in water for 14 day,group 3: flowable restorative were immersed in pH cycling solution,group 4: flowable restorative were immersed for 14 days in water . in day 15...
Background. The main etiology of endodontic treatment failure is caused by bacteries that stay i... more Background. The main etiology of endodontic treatment failure is caused by bacteries that stay in the root canal. E.faecalis is a bactery that is found as an etiology of endodontic treatment failure. Cell wall of this bacteria is containing Lipoteichoic acid (LTA). LTA can penetrate into the periradicular tissue, act as endotoxin in host and cause periradicular inflammation and destruction. It occurs due to the capability of TGF-ß to enhance the proliferation collagen and MMP-1 to stop the collagen formation. The ability of enterococcus faecalis in enhancing inflamation process cause host can not reach the homeostasis phase and performing an even bigger tissue damage. Purpose. The aim of this study is to know about the expression of of TGF-ß and MMP-1 during the periapical tissue damage due to induction of E.faecalis. Method. This study used laboratory experimental with the post test only control group design. A total of 27 male rats were randomly divided into 3 main groups. Group ...
Journal of Dentomaxillofacial Science, Feb 28, 2014
Stem cells provide new hope to fasten the healing process of various oral diseases including peri... more Stem cells provide new hope to fasten the healing process of various oral diseases including periodontal tissue (periodontitis). At present time, there is not yet found a material that can fix the teeth perfectly. Adult stem cells from bone marrow widely used for treatment. Numbers of stem cell are limited so growth factor is necessary to promote proliferation of stem cells. Growth factor that has been used is expensive and difficult to get. Because of these constraints, it is necessary to develop alternative uses of medicinal plants as growth factor that accelerates wound healing process. This study is aimed to proven what extent the potential Pegagan (Centella asiatica L) as a natural growth factors to stem cells. The ability of proliferation and osteogenic differentiation in mesenchymal stem cells (MSC) was analyzed by adding terpenoid fraction of Centella asiatica. MTT assay data showed that the addition of Pegagan on cultured MSCs can increase stem cell proliferation. Pegagan increases the ability of MSC proliferation and differentiate into osteoblasts It is concluded that pegagan increase the ability of MSC proliferation and differentiate into osteoblasts.
Catfish (Clarias batrachus) oil contain the highest amount of omega-3-PUFA among other freshwater... more Catfish (Clarias batrachus) oil contain the highest amount of omega-3-PUFA among other freshwater fish. The omega-3-PUFA in fish oil produced eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA). It is known that EPA and DHA in essentials fatty acid (EFA) could improve BMP-2 expression. Bone morphogenetic protein-2 (BMP-2) is bone stimulator which capable of inducing differentiation of mesenchymal cells into osteoblast, stimulating bone formation in wound healing process of dental extraction. Purpose: To prove the increasing expression of BMP-2 after catfish (Clarias batrachus) oil application. Methods: We have used the post test only design in this research. There have been 21 Rattus novergicus as research samples, and those were divided into 3 groups, group KK as control, group KP1 was given catfish (Clarias batrachus) oil in 5% concentration, and group KP2 was given catfish (Clarias batrachus) oil in 10% concentration. Catfish (Clarias batrachus) oil were applied into the s...
Clinical, Cosmetic and Investigational Dentistry, 2020
Background: Post-tooth extraction socket preservation is necessary due to alveolar bone resorptiv... more Background: Post-tooth extraction socket preservation is necessary due to alveolar bone resorptive patterns through regenerative dentistry approaches that involve the use of stem cells, scaffold and growth factor. Stem cells derived from human exfoliated deciduous teeth (SHED) are known to potentially possess the osteogenic ability. Meanwhile, carbonate apatite scaffold (CAS) can act as a biocompatible scaffold capable of supporting mesenchymal stem cells (MSCs) to proliferate and differentiate optimally. The aim of this study is to investigate the expression of bone morphogenic protein-2 and 7 (BMP2, BMP7) and Matrix Metalloproteinase-8 (MMP-8) after the transplantation of SHED-incorporated CAS during in vivo bone remodeling. Material and Methods: A total of 14 healthy, male, Wistar rats, whose mandible anterior teeth were extracted by means of sterile needle holder clamps, constituted the subjects of this study of alveolar bone defects. Two research groups were created: a control group (CAS) as group I and an experimental group (CAS + SHED) as group II. SHED with a density of 10 6 cells were incorporated into CAS before being transplanted into the experimental group. After 7 days, all the animals were sacrificed and their mandible anterior region extracted. The BMP2, BMP7 and MMP-8 expression were subsequently analyzed by means of immunostaining. An unpaired t-test was conducted to analyze the treatment and control group (p<0.01) data. Results: The expression of BMP-2 and BMP-7 was higher in group II compared to group I. Meanwhile, the level of MMP-8 was lower in group II than group I. There was greater significant increased expression of BMP-2 and BMP-7 expression in Group II compared to Group I. There was significant decreased expression of MMP-8 between group II than group I (p<0.01). Conclusion: SHED-incorporated CAS can enhance BMP-2 and BMP-7 expression while attenuating MMP-8 expression during in vivo alveolar bone remodeling.
Background: EndoActivator, a sonically-driven canal irrigation system (Dentsply Tulsa Dental Spec... more Background: EndoActivator, a sonically-driven canal irrigation system (Dentsply Tulsa Dental Specialties, Tulsa, OK), has been developed for activating root canal irrigants, and has recently been released onto the market. Purpose: To obtain an initial understanding of bubbles generation of electrical EndoActivator tips during activation of the irrigant in a transparent root canal model and a modified extracted tooth. Methods: A modified extracted tooth and a straight glass model were filled with a solution containing 17% EDTA or 3% NaOCl. A medium activator tip 22-mm polymer noncutting #25, 0.04 file driven by an electrical sonic hand-piece at 190 Hz (highest level) induced pressure waves that produced macro- and micro-bubbles. The physical mechanisms involved were visualized using a Miro 320S high-speed imaging system (Phantom, Wayne, NJ, USA) with high temporal and spatial resolutions. The imaging system acquired images at 25,000 frames per second with 320×x240 pixels per image, a...
This article was migrated. The article was not marked as recommended. Purpose: Research-informed ... more This article was migrated. The article was not marked as recommended. Purpose: Research-informed teaching is a crucial component of university education, and exposure to lectures by world scientists is one way to motivate undergraduate students to develop the future of dentistry. This study aimed to examine dental students' perceptions of international videoconferencing lectures on basic/clinical research, as well as Japanese dental students' perceptions of the use of videoconferencing lectures in large classes. Methods: We administered a questionnaire survey to senior undergraduate students in dental schools that participated in videoconferencing lectures in 2015/2016 in Japan (n=92) and Indonesia (n=175) and analyzed the data quantitatively and qualitatively. Results: Half of the Japanese and Indonesian students reported being motivated by the presence of foreign dental students in the videoconferencing lectures although there were significant differences between the Japan...
Background: Tissue engineering using Stem cell from Human Exfoliated Deciduous Teeth (SHED) and a... more Background: Tissue engineering using Stem cell from Human Exfoliated Deciduous Teeth (SHED) and a natural biomaterials biomaterial scaffold has become a promising therapy for the alveolar bone defect. The aim of this study was to analyze the Osteoprotegerin (OPG) and Receptor Activator of NF-Κb ligand (RANKL) expression after the application of Hydroxyapatite scaffold and SHED. Methods: A laboratory experimental research with a post test-only control group. 14 male Wistar rats weighing from 260 to 280 g were used as the animal study. The animals were randomly assigned to an experimental group Hydroxyapatite scaffold (group I) and Hydroxyapatite scaffold combined with SHED (group II). The alveolar bone defect in the animal study model was affected by extracting anterior mandible teeth. Immunostaining was performed after 8 weeks in order to facilitate the examination of OPG and RANKL expression. Data were analyzed by independent t test. The correlation between OPG and RANKL expression...
tujuan dari pengobatan regeneratif jaringan pulpa adalah untuk meregenerasi jaringan normal pada ... more tujuan dari pengobatan regeneratif jaringan pulpa adalah untuk meregenerasi jaringan normal pada daerah pertemuan antara pulpa dan dentin. jaringan ini merupakan jaringan yang meregulasi pembentukan dentin tersier. jaringan pulpa terdirivdari sel progenitor yang berpoliferasic dan berdiferensiasi menjadi odontoblas. odontoblas yang rusak dapat diganti oleh cell yang menyerupai odontoblas yang berassal dari sel fibroblast pulpa. dalam proses ini beberapa pembentukan dentin reparatif. exegenous FGF-2 akan berikatan dengan reseptornya pada permukaan sel fibroblas yang akan akan menginduksi cell signaling pada tulisan ini akan dibahas mengenai peran FGF-2 dalam proliferasi sel fibroblast yang akan menginduksi cell signaling. pada tulisan ini akan dibahas mengenai peran FGF-2 dalam proliferasi sel fibroblas pulpa. peran FGF-2 sangat pending pada proses pembentukan dentin reparatif. FGF-2 meningkatkan proliferasi dan diferensiasi sel fibroblas pulpa. maj. ked.gi; desember 2008 ;15(@): 191...
Background: Alveolar bone defects in children still have a high incidence. Conventional bone graf... more Background: Alveolar bone defects in children still have a high incidence. Conventional bone graft technique that has been used as a defect therapy is still not effective, so new techniques with tissue engineering approach are needed. Bone Morphogenetic Protein 4 (BMP4) as one of the indicators of osteogenic differentiation has not been widely studied, especially in the transplantation with combination of Stem Cells from Human Exfoliated Deciduous (SHED) and carbonate apatite. Aim and Objectives: This research aimed to determine the expression of BMP4 after SHED and carbonate apatite transplantation on Rattus norvegicus. Material and Methods: The combinations of SHED and carbonate apatite were transplanted on alveolar bone defects of 4 rats (Rattus norvegicus) as the treatment groups and another 4 rats were transplanted with carbonate apatite as the control groups. After 21 days, staining with Hematoxylin Eosin (HE) and Immunohistochemistry (IHC) BMP4 was performed. Results: BMP4 ex...
Stem cells can give a new hope to accelerate wound healing process and can also be used for the t... more Stem cells can give a new hope to accelerate wound healing process and can also be used for the treatment of various diseases, including diseases on periodontal tissues (periodontitis) since there is still not any materials known to be able to attach wobbly teeth perfectly. Adult stem cells derived from bone marrow have widely been used nowadays. However, the number of stem cells is limited, consequently, growth factors are necessary to increase the proliferation of stem cells. Unfortunately, growth factors that have been used are still expensive and difficult to obtain. Therefore, an alternative to the use of growth factors from natural materials that can potentially accelerate wound healing process is necessary to be developed. Purpose: Golden Sea Cucumbers (Stichopus hermanii) is rich in both growth factors that can repair damaged cells and protein that reaches up to 82% of all components of the golden sea cucumbers, 80% of which is collagen. Therefore, this research aims to prov...
Periodontal regeneration, especially regeneration of alveolar bone, remains difficult to achieve ... more Periodontal regeneration, especially regeneration of alveolar bone, remains difficult to achieve with the result that alternative therapies for achieving periodontal regeneration are still lacking. Tissue engineering using stem cells and biomaterial scaffold represents an alternative for solving this problem. Stem cells from human exfoliated deciduous teeth (SHED) are one source of adult stem cells which can proliferate and differentiate into many types of specific cells. Hydroxyapatite (HA) constitutes a bioactive scaffold possessing osseointegration, osteoconduction and osteogenesis properties when used as bone graft. The aim of this study was to analyze osteocalcin (OCN) and osteopontin (OPN) expression after the application of a combination of HA and SHED to alveolar bone defects. Eight experimental animals were divided into two groups. The first group was transplanted with HA only, while the second was transplanted with HA/SHED. The expression of OCN and OPN was examined 21 day...
The pulp chamber is mostly infected by anaerob gram negative bacteria and one of them is Porphyro... more The pulp chamber is mostly infected by anaerob gram negative bacteria and one of them is Porphyromonas gingivalis. The cell wall ofthis bacteria containing Lipopolysaccharide (LPS). LPS can penetrate to the periradicular tissue, act as endotoxin in host and causeperiradicular inflammation that leading to bone destruction. Bone destruction occur is due to the inflammation process that mediatedby immune system. This inflammation is mediated by immune system that can be found the cytokin pro-inflammatory infiltrated in thecell has an important role in bone destruction is known as osteoclast. Osteoclasts are multinucleated, terminally differentiated cellswhich play an essential role in bone resorption. Binding RANK with RANKL are increasing during the osteoclastogenesis process. Themain purpose of this study was to determine the increasing number of osteoclast in periapical tissue that induced by LPS. This studyused laboratory experimental with the post test only control group design. A...
Stem cells gave a new way to accelerate healing progress because the stem cells can be utilized f... more Stem cells gave a new way to accelerate healing progress because the stem cells can be utilized for the treatment of various degenerative diseases including periodontitis. The number of stem cells is limited; therefore, a growth factor is needed to increase stem cell proliferation. Growth factor that has been used is still expensive and difficult to obtain so it is necessary to develop an alternative use of growth factors from natural materials that potentially accelerate wound healing. Mangosteen (Gracinia mangostana L) contains xanthones which are flavonoid compounds that can activate kinase protein, as growth factor stem cells. To discover the potential effect of mangosteen skin extract (Gracinia mangostana L) as growth factor of stem cells. Proliferative ability test of mangosteen skin (Gracinia mangostana L) as a growth factor for mesenchymal stem cell (MSC) using MTT assay with FGF comparator. Test of osteogenic differentiation using Alizarin Red coloring. Viability cells in M...
Background. Chlorhexidine gluconate is one of endodontic irrigants that has excellence capability... more Background. Chlorhexidine gluconate is one of endodontic irrigants that has excellence capability to penetrate into dentin tubules and kill the pathogenic bacteria there. On the other hand, chlorhexidine gluconate has side effects to cause allergic reactions of the tissue and discoloration of the teeth. Xanthone from mangosteen pericarp can be considered as a natural alternative irrigant that usually has a good tolerance to the body. Purpose. The aim of this study compared the cytotoxicity between 0,78% xanthone from mangosteen pericarp and 0,2% chlorhexidine gluconate toward BHK-21 fibroblast cells. Methods. This study used experimental post-test only control group design. Xanthone from mangosteen pericarp preliminary cytotoxicity tested in various concentrations. Xanthone from mangosteen pericarp classified as a non-toxic concentration at 0,78%. Cytotoxicity of 0,78% xanthone from mangosteen pericarp compare with cytotoxicity of 0,2% chlorhexidine gluconate using MTT assay method...
Background: Stem cells from human exfoliated deciduous teeth (SHED) seeded in carbonate apatite s... more Background: Stem cells from human exfoliated deciduous teeth (SHED) seeded in carbonate apatite scaffold (CAS) may have multiple functions that could be used to regenerate the alveolar bone defects. The purpose of this study is to examine the ability of SHED and CAS in alveolar bone defects using an immunohistochemical analysis. Methods: ten three-month-old healthy male Wistar rats (R. novergicus) that weighed between 150–250 grams (g) were used as animal models. A simple blind random sampling method was used to select the sample that was assigned to the study group for CAS and SHED seeded in CAS (n=5). The animal study model of the alveolar bone was established by extracting the anterior mandible teeth. Rodent anesthesia was applied to relieve the pain during the procedure for all test animals. Immunohistochemistry was performed after seven days to facilitate the examination of the receptor activator of NF-κβ ligand (RANKL), osteoprotegrin (OPG), transforming growth factor-β (TGF-β...
Background: Enterococcus faecalis is one of the most common bacteria in root canal after endodont... more Background: Enterococcus faecalis is one of the most common bacteria in root canal after endodontic treatment. This species is found in about 77% of the cases that resistant to treatment. It is necessary to develop an alternative for intracanal dressing, one of the potential substance is natural ingredients. The stem of ambonese banana (Musa paradisiaca var. sapientum) extract contains active substances such as tannin, flavonoid, alkaloid, saponin, and antraquinone, which have been known for their antibacterial potency. Purpose: The aim of this study was to determine the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of ambonese banana stem (Musa paradisiaca var. sapientum) extract against Enterococcus faecalis. Method: This research was a laboratory experimental study. Ambonese banana stem extract was made by maceration method with ethanol 96% and certain dilution was performed to obtain various concentration. Value of MIC and MBC of ambonese b...
Background: Cleanliness of cavity is considered important for a restoration. Smear layer formed a... more Background: Cleanliness of cavity is considered important for a restoration. Smear layer formed after cavity preparation should be removed in order not to disrupt the bond adhesion between restorative materials and dental cavities. Saponins contained in mangosteen pericarp (Garcinia mangostana L.) have surfactant properties that can eliminate the smear layer assessed. 6% citric acid is a chelating agent which can eliminate the inorganic particles of the smear layer. Until now, the research on the differences of 0,78% saponin from mangosteen pericarp extract and 6% citric acid for cleanliness of cavity has never been done. Purpose: To see the differences between 0,78% saponin from mangosteen pericarp extract and 6% citric acid as cavity cleanser. Method: Eighteen human teeth with complete crown, no caries, and no fractures were randomized in 3 groups (n≥6), in this experiment use (n=6). The cavity was prepared using wheels bur for hand use instrument. After instrumentation, each cav...
Background:Enterococcus faecalis (E. faecalis) is a microorganism that is commonly found in endod... more Background:Enterococcus faecalis (E. faecalis) is a microorganism that is commonly found in endodontic failure treatment, this due to several characteristics of E.faecalis which has the capabillity to living in environments with high salt levels, high temperature, and pH broad spectrum. Bacteria in biofilms form is one of the adaptive process that allows bacteria to survive in an environment with low nutrients in the root canals. Bacteria in biofilms form have different characteristics from planktonic form, resistance to phagocytic cells and drugs, which can effect to persistent infection. Mangosteen (Garcinia mangostana) has many benefits, especially on the pericarp of the fruit contains alkaloids, tannins, phenolics, flavonoids, and triterpenoids. Flavonoids are the largest group of phenolic compounds that have a nature effectively inhibit the growth of viruses, bacteria, and fungi. Purpose:Purpose of this study wasto find out the role of the antibiofilm of the flavonoid in garcin...
Background : Dentinal hypersensitivity (DH) is a painful clinical condition and is characterized ... more Background : Dentinal hypersensitivity (DH) is a painful clinical condition and is characterized by pain arising from exposed dentin in response to various stimuli. Various treatment modalities are available to treat dentinal hypersensitivity which include at-home and in-office treatment. At home treatment generally consists of a variety of dentrifices containing different constituents like strontium chloride and bioactive glass (Novamin). These agents cause occlusion of dentinal tubules thereby reducing hypersensitivity.Purpose: This study was to evaluate the effects of different desensitizing dentifrices on dentinal tubule occlusion by scanning electron microscopy (SEM) of strontium chloride and bioactive glass (Novamin) pastes in the treatment of dentinehypersensitivity (DH) Methods: sixteen extractedspecimens from bovine incisors teeth with randomized into 2 groups (n=8). The crowns were removed from the root and the crown were sectioned longitudinally into two parts (in a me...
Back Ground: Adherence of Stretococcus mutans biofilm (S. mutans) to the surface of dental resto... more Back Ground: Adherence of Stretococcus mutans biofilm (S. mutans) to the surface of dental restorative materials is considered an important step in the development of secondary caries and periodontal disease. There are two type of nanofil composite: universal restorative and flowable restorative. That have different characteristic to induce S.mutans biofilm adherent in it surface. Purpose: The aim of this study was to compare the adherence of S. mutans biofilm to two types of nanofil restorative materials, flowable restorative and universal restorative.. Materials and Methode: 32 disc-shaped specimens (∅ = 5.0 mm / thickness = 2.0 mm) of two types composite were divided to 4 groups (n = 8): group 1; universal restorative were immersed in pH cycling solution for 14 days ,group 2; Universal restorative were immersed in water for 14 day,group 3: flowable restorative were immersed in pH cycling solution,group 4: flowable restorative were immersed for 14 days in water . in day 15...
Background. The main etiology of endodontic treatment failure is caused by bacteries that stay i... more Background. The main etiology of endodontic treatment failure is caused by bacteries that stay in the root canal. E.faecalis is a bactery that is found as an etiology of endodontic treatment failure. Cell wall of this bacteria is containing Lipoteichoic acid (LTA). LTA can penetrate into the periradicular tissue, act as endotoxin in host and cause periradicular inflammation and destruction. It occurs due to the capability of TGF-ß to enhance the proliferation collagen and MMP-1 to stop the collagen formation. The ability of enterococcus faecalis in enhancing inflamation process cause host can not reach the homeostasis phase and performing an even bigger tissue damage. Purpose. The aim of this study is to know about the expression of of TGF-ß and MMP-1 during the periapical tissue damage due to induction of E.faecalis. Method. This study used laboratory experimental with the post test only control group design. A total of 27 male rats were randomly divided into 3 main groups. Group ...
Journal of Dentomaxillofacial Science, Feb 28, 2014
Stem cells provide new hope to fasten the healing process of various oral diseases including peri... more Stem cells provide new hope to fasten the healing process of various oral diseases including periodontal tissue (periodontitis). At present time, there is not yet found a material that can fix the teeth perfectly. Adult stem cells from bone marrow widely used for treatment. Numbers of stem cell are limited so growth factor is necessary to promote proliferation of stem cells. Growth factor that has been used is expensive and difficult to get. Because of these constraints, it is necessary to develop alternative uses of medicinal plants as growth factor that accelerates wound healing process. This study is aimed to proven what extent the potential Pegagan (Centella asiatica L) as a natural growth factors to stem cells. The ability of proliferation and osteogenic differentiation in mesenchymal stem cells (MSC) was analyzed by adding terpenoid fraction of Centella asiatica. MTT assay data showed that the addition of Pegagan on cultured MSCs can increase stem cell proliferation. Pegagan increases the ability of MSC proliferation and differentiate into osteoblasts It is concluded that pegagan increase the ability of MSC proliferation and differentiate into osteoblasts.
Catfish (Clarias batrachus) oil contain the highest amount of omega-3-PUFA among other freshwater... more Catfish (Clarias batrachus) oil contain the highest amount of omega-3-PUFA among other freshwater fish. The omega-3-PUFA in fish oil produced eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA). It is known that EPA and DHA in essentials fatty acid (EFA) could improve BMP-2 expression. Bone morphogenetic protein-2 (BMP-2) is bone stimulator which capable of inducing differentiation of mesenchymal cells into osteoblast, stimulating bone formation in wound healing process of dental extraction. Purpose: To prove the increasing expression of BMP-2 after catfish (Clarias batrachus) oil application. Methods: We have used the post test only design in this research. There have been 21 Rattus novergicus as research samples, and those were divided into 3 groups, group KK as control, group KP1 was given catfish (Clarias batrachus) oil in 5% concentration, and group KP2 was given catfish (Clarias batrachus) oil in 10% concentration. Catfish (Clarias batrachus) oil were applied into the s...
Clinical, Cosmetic and Investigational Dentistry, 2020
Background: Post-tooth extraction socket preservation is necessary due to alveolar bone resorptiv... more Background: Post-tooth extraction socket preservation is necessary due to alveolar bone resorptive patterns through regenerative dentistry approaches that involve the use of stem cells, scaffold and growth factor. Stem cells derived from human exfoliated deciduous teeth (SHED) are known to potentially possess the osteogenic ability. Meanwhile, carbonate apatite scaffold (CAS) can act as a biocompatible scaffold capable of supporting mesenchymal stem cells (MSCs) to proliferate and differentiate optimally. The aim of this study is to investigate the expression of bone morphogenic protein-2 and 7 (BMP2, BMP7) and Matrix Metalloproteinase-8 (MMP-8) after the transplantation of SHED-incorporated CAS during in vivo bone remodeling. Material and Methods: A total of 14 healthy, male, Wistar rats, whose mandible anterior teeth were extracted by means of sterile needle holder clamps, constituted the subjects of this study of alveolar bone defects. Two research groups were created: a control group (CAS) as group I and an experimental group (CAS + SHED) as group II. SHED with a density of 10 6 cells were incorporated into CAS before being transplanted into the experimental group. After 7 days, all the animals were sacrificed and their mandible anterior region extracted. The BMP2, BMP7 and MMP-8 expression were subsequently analyzed by means of immunostaining. An unpaired t-test was conducted to analyze the treatment and control group (p<0.01) data. Results: The expression of BMP-2 and BMP-7 was higher in group II compared to group I. Meanwhile, the level of MMP-8 was lower in group II than group I. There was greater significant increased expression of BMP-2 and BMP-7 expression in Group II compared to Group I. There was significant decreased expression of MMP-8 between group II than group I (p<0.01). Conclusion: SHED-incorporated CAS can enhance BMP-2 and BMP-7 expression while attenuating MMP-8 expression during in vivo alveolar bone remodeling.
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