PRACTICAS DE BIOINFORMATICA E.P.
BIOLOGIA UNSA
UNIVERSIDAD NACIONAL DE SAN AGUSTÍN
FACULTAD DE CIENCIAS BIOLÓGICAS
ESCUELA PROFESIONAL DE BIOLOGÍA
PRÁCTICAS DE BIOINFORMÁTICA
TEMA 1: BASES DE DATOS
GRUPO: viernes 11:00 / 14:20 pm
INTEGRANTES:
AGUILAR CAHUANA MIGUEL
ALVAREZ GONGORA MALHU
PALMA VILCA FERNANDA
SANCHEZ CASTRO FLOR LEONOR MAYTE
AREQUIPA 2021
PRACTICAS DE BIOINFORMATICA E.P. BIOLOGIA UNSA
PRACTICA 6: ALINEAMIENTO MULTIPLE
Objetivo:
Usar software BioEdit y Mega para alineamiento multiple
Hacer el alineamiento de la secuencias del gen CT099
de Lycopersicon pimpinellifolium
Introducción:
Hablamos de alineamientos multiples (MSA) cuando tratamos con mas de dos secuencias.
Este tipo de alineamientos requiere un abordaje más complejo que el de pares de
secuencias ya que el uso directo de los estadísticos de alineamiento global y local requiere
una gran capacidad de cálculo y solo es aplicable a problemas con muy pocas secuencias.
Los algoritmos de construcción progresiva consiste en primero realizar alineamientos de
dos en dos. A partir de estos alineamientos se construye una matriz de distancias entre las
secuencias y un árbol guía basado en estas distancias. Mediante este árbol podemos
encontrar las parejas de secuencias más similares Los programas más utilizados se basan
en este tipo de estrategias. No nos garantizan que el alineamiento obtenido sea el mejor
posible, pero son capaces de encontrar una solución óptima de forma muy eficaz. Ejemplos
de programas basados en estos métodos heurísticos: ClustalW y T-coffe.
Actividades:
1. Instalar el software BioEdit
Caracterizar el software:
Este es un software editor de alineación de secuencias biológicas que se
creó específicamente para el sistema operativo de Windows. Es muy fácil
hacer la alineación y manipulación de secuencias. Esta es la aplicación
definitiva que pueden utilizar aquellos que están interesados en
secuencia biológica y demostró ser muy precisa a diferencia de otros
programas de software similares.
Este programa presta un diseño muy interactivo, mentor que puede
moverse y encontrar su camino fácilmente sin la ayuda de nadie. Es a la
vez intuitivo y con un diseño excelente, no le resultada difícil encaminar
su secuencia con esta increíble aplicación. Alguna de sus características
interesantes incluye varios modos de dibujo de plásmidos, alineación
manual, mapeo de restricción.
PRACTICAS DE BIOINFORMATICA E.P. BIOLOGIA UNSA
Todas estas características y más en las cosas que debe esperar de ese
maravilloso software, ya que hace que la secuenciación se mucho más
fácil.
2. Caracterizar la secuencias obtenidas del fichero fichero seq_dna.fasta
3. Copiar las 10 secuencias en formato Fasta
>gi|73808525|gb|DQ103386.1| Lycopersicon pimpinellifolium CT099 (CT099) gene, CT099-7272a allele,
partial cds
CTTAACCCTTCTGTTGATTACAACACTTGGTCTCAGCATATGAGATTTATAATCAACGACTCTGTTCGTAAGTCTTAACATGT
CTCTCTTTATTTATTATTGCTTGAAGAGTTTGAACAATATAATTGGTAAACGAGTTTTTTCTATAGTGTTGATACTTTTTTTTT
TGTGTGTGTGCGCTTTTTTTAGTGTTTAAGTATAAGCAAGGAGCAGATTCTGTGTTGGAGGTGAGTAAGGATGATTATGA
TAAGTGCAATACTGGAAATCCAATTAAGAAAATGGAGGATGGAAACTCGATTTTCACGTTGGATCGGTCTGGTCCTTTTTA
CTTTATCAGTGGGAACAAAGATAACTGTGATAAAGGCCAAAAGTTGCAAATTGTTGTGATTTCTGCTAGGAATCAAGGTA
AACCACCACAAACACCGGCACCGGCAGTTGCTCCACCTAGTAATGGATCAACTACACCATCTACTCCTTCTGGAGGTACGC
GGAATCAGGATTTTTATTTTATTCCCGTTCAATTTTACGTTGACATCTCCTTATGTGGTTTGCGAGTTATTGTATCTAAGGAT
AAAAATTACATGATTCGATTGAGAACTTACGTGATTAATGCAGGCTCAACTCCGGCAGCTGCTCCGTCTAAAGGATCGGG
TACACCGTCGGCACCTTCAGCTAACGCTCCGGCGGGATCATCTAAACCGGGTGCATCGTCACCTAATGGGGCACCGGTAT
CTACTCCTGCAGGTAAATGTAGAATTTCGATTTGATGGATTCAACGTTTAATTTCGTTCGATTTTTAACGTATTGAACGTAA
ATTGAATCATATAGTTTATCTGTTTGTTCAGGTAAATCACCAACCTCATCACCTACACCTTCCGGTTCAACCGCATCTCCACC
ATCTCCTGCGACCGTAACTCCGGCAATGTCTCCGGTAGCAAATGGACCGTCAACTAGTACACCAGGTTCTTCTTCACCTGT
AGCAGGCGGACCATCGTCAGGAAGCGGTATTGCACCGTCGGCAAGCGGACCATCTGGAAGCGCTATTGCACCGGCGGCA
GACGGACCAACAGTAAGTATGTCACCGGGACCATCGGCAGGAGGACCATTGGCAGGCGGACCATCGGAGGGAGCACCG
GGAAGTAGTGCTTTAGGTCCCGGCGGCAGCAATACTCCGGCGGACATCAATACGCCAGCTGGCGCACCAGAAAACCCAA
ATTCATTTGCAGTGAAGGCATTTACTCCATCAGTTGTACTTGTG
>gi|73808527|gb|DQ103387.1| Lycopersicon pimpinellifolium CT099 (CT099) gene, CT099-7272b allele,
partial cds
CAACACTTGGTCTCAGCATATGAGATTTATAATCAACGACTCTGTTCGTAAGTCTTAACATGTCTCTCTTTATTTATTATTGC
TTGAAGAGATCGAACAATATAATTGGTAAACGAGTTTTTTCTATATGTTGATACTTTTTTTTTTGTGTGTGCGCGCTTTTTTT
AGTGTTTAAGTATAAGCAAGGAGCAGATTCTGTGTTGGAGGTGAGTAAGGATGATTATGATAAGTGCAATACTGGAAAT
CCAATTAAGAAAATGGAGGATGGAAACTCGATTTTCACGTTGGATCGGTCTGGTCCTTTTTACTTTATCAGTGGGAACAAA
GATAACTGTGATAAAGGCCAAAAGTTGCAAATTGTTGTGATTTCTGCTAGGAATCAAGGTAAACCACCACAAACACCGGC
ACCGGCAGTTGCTCCACCTAGTAATGGATCAACTACACCATCTACTCCTTCTGGAGGTACGCGGAATCAGGATTTTTATTTT
ATTCCCGTTCAATTTTACGTTGACATCTCCTTATGTGGTTTGCGAGTTATTGTATCTAAGGATAAAAATTACATGATTCGATT
GAGAACTTACGTGATTAATGCAGGCTCAACTCCGGCAGCTGCTCCGTCTAAAGGATCGGGTACACCGTCGGCACCTTCAG
CTAACGCTCCGGCGGGATCATCTAAACCGGGTGCATCGTCACCTAATGGGGCACCGGTATCTACTCCTGCAGGTAAATGT
AGAATTTCGATTTGATGGATTCAACGTTTAATTTCGTTCGATTTTTAACGTATTGAACGTAAATTGAATCATATAGTTTATCT
GTTTGTTCAGGTAAATCACCAACCTCATCACCTACACCTTCCGGTTCAACCGCATCTCCACCATCTCCTGCGACCGTAACTCC
GGCAATGTCTCCGGTAGCAAATGGACCGTCAACTAGTACACCAGGTTCTTCTTCACCTGTAGCAGGCGGACCATCGTCAG
GAAGCGGTATTGCACCGGCGGCAAGCGGACCATCTGGAAGCGCTATTGCACCGGCGGCAGACGGACCAACAGTAAGTAT
PRACTICAS DE BIOINFORMATICA E.P. BIOLOGIA UNSA
GTCACCGGGACCATCGGCAGGAGGACCATTGGCAGGCGGACCATCGGAGGGAGCACCGGGAAGTAGTGCTTTAGGTCC
CGGCGGCAGCAATACTCCGGCGGACATCAATACGCCAGCTGGCGCACCAGAAAACCCAAATTCATTTGCAGTGAAGGCAT
TTACTCCATCAGTTGTACTTGTG
>gi|73808529|gb|DQ103388.1| Lycopersicon pimpinellifolium CT099 (CT099) gene, CT099-7274a allele,
partial cds
ACCCTTCTGTTGATTACAACACTTGGTCTCAGCATATGAGATTTATAATCAACGACTCTGTTCGTAAGTCTTAACATGTCTCT
CTTTATTTATTATTGCTTGAAGAGTTTGAACAATATAATTGGTAAACGAGTTTTTTCTATATGTTGATACTTTTTTTTTTGTGT
GTGCGCGCTTTTTTTAGTGTTTAAGTATAAGCAAGGAGCAGATTCTGTGTTGGAGGTGAGTAAGGATGATTATGATAAGT
GCAATACTGGAAATCCAATTAAGAAAATGGAGGATGGAAACTCGATTTTCACGTTGGATCGGTCTGGTCCTTTTTACTTTA
TCAGTGGGAACAAAGATAACTGTGATAAAGGCCAAAAGTTGCAAATTGTTGTGATTTCTGCTAGGAATCAAGGTAAACCA
CCACAAACACCGGCACCGGCAGTTGCTCCACCTAGTAATGGATCAACTACACCATCTACTCCTTCTGGAGGTACGCGGAAT
CAGGATTTTTATTTTATTCCCGTTCAATTTTACGTTGACATCTCCTTATGTGGTTTGCGAGTTATTGTATCTAAGGATAAAAA
TTACATGATTCGATTGAGAACTTACGTGATTAATGCAGGCTCAACTCCGGCAGCTGCTCCGTCTAAAGGATCGGGTACACC
GTCGGCACCTTCAGCTAACGCTCCGGCGGGATCATCTAAACCGGGTGCATCGTCACCTAATGGGGCACCGGTATCTACTC
CTGCAGGTAAATGTAGAATTTCGATTTGATGGATTCAACGTTTAATTTCGTTCGATTTTTAACGTATTGAACGTAAATTGAA
TCATATAGTTTATCTGTTTGTTCAGGTAAATCACCAACCTCATCACCTACACCTTCCGGTTCAACCGCATCTCCACCATCTCC
TGCGACCGTAACTCCGGCAATGTCTCCGGTAGCAAATGGACCGTCAACTAGTACACCAGGTTCTTCTTCACCTGTAGCAGG
CGGACCATCGTCAGGAAGCGGTATTGCACCGTCGGCAGGCGGACCATCTGGAAGCGCTATTGCACCGGCGGCAGACGGA
CCAACAGTAAGTATGTCACCGGGACCATCGGCAGGAGGACCATTGGCAGGCGGACCATCGGAGGGAGCACCGGGAAGT
AGTGCTTTAGGTCCCGGCGGCAGCAATACTCCGGCGGACATCAATACGCCAGCTGGCGCACCAGAAAACCCAAATTCATT
TGCAGTGAAGGCATTTACTCCATCAGTTGTACTTGTG
>gi|73808531|gb|DQ103389.1| Lycopersicon pimpinellifolium CT099 (CT099) gene, CT099-7274b allele,
partial cds
CACTTGGTCTCAGCATATGAGATTTATAATCAACGACTCTGTTCGTAAGTCTTAACATGTCTCTCTTTATTTATTATTGCTTG
AAGAGTTTGAACAATATAATTGGTAAACGAGTTTTTTCTATATGTTGATACTTTTTTTTTTGTGTGTGCGCGCTTTTTTTAGT
GTTTAAGTATAAGCAAGGAGCAGATTCTGTGTTGGAGGTGAGTAAGGATGATTATGATAAGTGCAATACTGGAAATCCA
ATTAAGAAAATGGAGGATGGAAACTCGATTTTCACGTTGGATCGGTCTGGTCCTTTTTACTTTATCAGTGGGAACAAAGAT
AACTGTGATAAAGGCCAAAAGTTGCAAATTGTTGTGATTTCTGCTAGGAATCAAGGTAAACCACCACAAACACCGGCACC
GGCAGTTGCTCCACCTAGTAATGGATCAACTACACCATCTACTCCTTCTGGAGGTACGCGGAATCAGGATTTTTATTTTATT
CCCGTTCAATTTTACGTTGACATCTCCTTATGTGGTTTGCGAGTTATTGTATCTAAGGATAAAAATTACATGATTCGATTGA
GAACTTACGTGATTAATGCAGGCTCAACTCCGGCAGCTGCTCCGTCTAAAGGATCGGGTACACCGTCGGCACCTTCAGCT
AACGCTCCGGCGGGATCATCTAAACCGGGTGCATCGTCACCTAATGGGGCACCGGTATCTACTCCTGCAGGTAAATGTAG
AATTTCGATTTGATGGATTCAACGTTTAATTTCGTTCGATTTTTAACGTATTGAACGTAAATTGAATCATATAGTTTATCTGT
TTGTTCAGGTAAATCACCAACCTCATCACCTACACCTTCCGGTTCAACCGCATCTCCACCATCTCCTGCGACCGTAACTCCG
GCAATGTCTCCGGTAGCAAATGGACCGTCAACTAGTACACCAGGTTCTTCTTCACCTGTAGCAGGCGGACCATCGTCAGG
AAGCGGTATTGCACCGTCGGCAGGCGGACCATCTGGAAGCGCTATTGCACCGGCGGCAGACGGACCAACAGTAAGTATG
TCACCGGGACCATCGGCAGGAGGACCATTGGCAGGCGGACCATCGGAGGGAGCACCGGGAAGTAGTGCTTTAGGTCCC
GGCGGCAGCAATACTCCGGCGGACATCAATACGCCAGCTGGCGCACCAGAAAACCCAAATTCATTTGCAGTG
>gi|73808543|gb|DQ103395.1| Lycopersicon pimpinellifolium CT099 (CT099) gene, CT099-7277b allele,
partial cds
ACCCTTCTGTTGATTACAACACTTGGTCTCAGCATATGAGATTTATAATCAACGACTCTGTTCGTAAGTCTTAACATGTCTCT
CTTTATTTATTATTGCTTGAAGAGTTTGAACAATATAATTGGTAAACGAGTTTTTTCTATATGTTGATACTTTTTTTTTTGTGT
GTGTGCGCTTTTTTTAGTGTTTAAGTATAAGCAAGGAGCAGATTCTGTGTGGAGGTGAGTAAGGATGATTATGATAAGTG
CAATACTGGAAATCCAATTAAGAAAATGGAGGATGGAAACTCGATTTTCACGTTGGATCGGTCTGGTCCTTTTTACTTTAT
CAGTGGGAACAAAGATAACTGTGATAAAGGCCAAAAGTTGCAAATTGTTGTGATTTCTGCTAGGAATCAAGGTAAACCAC
CACAAACACCGGCACCGGCAGTTGCTCCACCTAGTAATGGATCAACTACACCATCTACTCCTTCTGGAGGTACGCGGAATC
AGGATTTTTATTTTATTCCCGTTGCAATTTTACGTTGACATCTCCTTACGTAGTTTGCGAGTTATTGTATCTAAGGATTGAGA
ACTTACGTGATTAATGCAGGCTCAACTCCGGCAGCTGCTCCGTCTAAAGGATCGGGTACACCGTCGGCACCTTCAGCTAAC
GCTCCGGCGGGATCATCTAAACCGGGTGCATCGTCACCTAATGGGGCACCGGTATCTACTCCTGCAGGTAAATGTAGAAT
TTCGATTTGATGGATTCAACGTTTAATTTCGTTCGATTTTTAACGTATTGAACGTAAATTGAATCATATAGTTTATCTGTTTG
TTCAGGTAAATCACCAACCTCATCACCTACACCTTCCGGTTCAACCGCATCTCCACCATCTCCTGCGACCGTAACTCCGGCA
ATGTCTCCGGTAGCAAATGGACCGTCAACTAGTACACCAGGTTCTTCTTCACCTGTAGCAGGCGGACCATCGTCAGGAAG
CGGTATTGCACCGTCGGCAAGCGGACCATCTGGAAGCGCTATTGCACCGGCGGCAGACGGACCAACAGTAAGTATGTCA
CCGGGACCATCGGCAGGAGGACCATTGGCAGGCGGACCATCGGAGGGAGCACCGGGAAGTAGTGCTTTAGGTCCCGGC
PRACTICAS DE BIOINFORMATICA E.P. BIOLOGIA UNSA
GGCAGCAATACTCCGGCGGACATCAATACGCCAGCTGGCGCACCAGAAAACCCAAATTCATTTGCAGTGAAGGCATTTAC
TCCATCAGTTGTGCTTGTG
>gi|73808541|gb|DQ103394.1| Lycopersicon pimpinellifolium CT099 (CT099) gene, CT099-7277a allele,
partial cds
GGTCTCAGCATATGAGATTTATAATCAACGACTCTGTTCGTAAGTCTTAACATGTCTCTCTTTATTTATTATTGCTTGAAGAG
TTTGAACAATATAATTGGTAAACGAGTTTTTTCTATATGTTGATACTTTTTTTTTTGTGTGTGTGCGCTTTTTTTAGTGTTTAA
GTATAAGCAAGGAGCAGATTCTGTGTTGGAGGTGAGTAAGGATGATTATGATAAGTGCAATACTGGAAATCCAATTAAG
AAAATGGAGGATGGAAACTCGATTTTCACGTTGGATCGGTCTGGTCCTTTTTACTTTATCAGTGGGAACAAAGATAACTGT
GATAAAGGCCAAAAGTTGCAAATTGTTGTGATTTCTGCTAGGAATCAAGGTAAACCACCACAAACACCGGCACCGGCAGT
TGCTCCACCTAGTAATGGATCAACTACACCATCTACTCCTTCTGGAGGTACGCGGAATCAGGATTTTTATTTTATTCCCGTT
CAATTTTACGTTGACATCTCCTTACGTAGTTTGCGAGTTATTGTATCTAAGGATTGAGAACTTACGTGATTAATGCAGGCTC
AACTCCGGCAGCTGCTCCGTCTAAAGGATCGGGTACACCGTCGGCACCTTCAGCTAACGCTCCGGCGGGATCATCTAAAC
CGGGTGCATCGTCACCTAATGGGGCACCGGTATCTACTCCTGCAGGTAAATGTAGAATTTCGATTTGATGGATTCAACGTT
TAATTTCGTTCGATTTTTAACGTATTGAACGTAAATTGAATCATATAGTTTATCTGTTTGTTCAGGTAAATCACCAACCTCAT
CACCTACACCTTCCGGTTCAACCGCATCTCCACCATCTCCTGCGACCGTAACTCCGGCAATGTCTCCGGTAGCAAATGGACC
GTCAACTAGTACACCAGGTTCTTCTTCACCTGTAGCAGGCGGACCATCGTCAGGAAGCGGTATTGCACCGTCGGCAAGCG
GACCATCTGGAAGCGCTATTGCACCGGCGGCAGACGGACCAACAGTAAGTATGTCACCGGGACCATCGGCAGGAGGACC
ATTGGCAGGCGGACCATCGGAGGGAGCACCGGGAAGTAGTGCTTTAGGTCCCGGCGGCAGCAATACTCCGGCGGACAT
CAATACGCCAGCTGGCGCACCAGAAAACCCAAATTCATTTGCAGTGAAGGCATTTACTCCATCAGTTGTGCTTGTG
>gi|73808539|gb|DQ103393.1| Lycopersicon pimpinellifolium CT099 (CT099) gene, CT099-7276b allele,
partial cds
CTTAACCCTTCTGTTGATTACAACACTTGGTCTCAGCATATGAGATTTATAATCAACGACTCTGTTCGTAAGTCTTAACATGT
CTCTCTTTATTTATTATTGCTTGAAGAGTTTGAACAATATAATTGGTAAACGAGTTTTTTCTATATGTTGATACTTTTTTTTTT
GTGTGTGTGCGCTTTTTTTAGTGTTTAAGTATAAGCAAGGAGCAGATTCTGTGTTGGAGGTGAGTAAGGATGATTATGAT
AAGTGCAATACTGGAAATCCAATTAAGAAAATGGAGGATGGAAACTCGATTTTCACGTTGGATCGGTCTGGTCCTTTTTAC
TTTATCAGTGGGAACAAAGATAACTGTGATAAAGGCCAAAAGTTGCAAATTGTTGTGATTTCTGCTAGGAATCAAGGTAA
ACCACCACAAACACCGGCACCGGCAGTTGCTCCACCTAGTAATGGATCAACTACACCATCTACTCCTTCTGGAGGTACGCG
GAATCAGGATTTTTATTTTATTCCCGTTCAATTTTACGTTGACATCTCCTTACGTAGTTTGCGAGTTATTGTATCTAAGCATT
GAGAACTTACGTGATTAATGCAGGCTCAACTCCGGCAGCTGCTCCGTCTAAAGGATCGGGTACACCGTCGGCACCTTCAG
CTAACGCTCCGGCGGGATCATCTAAACCGGGTGCATCGTCACCTAATGGGGCACCGGTATCTACTCCTGCAGGTAAATGT
AGAATTTCGATTTGATGGATTCAACGTTTAATTTCGTTCGATTTTTAACGTATTGAACGTAAATTGAATCATATAGTTTATCT
GTTTGTTCAGGTAAATCACCAACCTCATCACCTACACCTTCCGGTTCAACCGCATCTCCACCATCTCCTGCGACCGTAACTCC
GGCAATGTCTCCGGTAGCAAATGGACCGTCAACTAGTACACCAGGTTCTTCTTCACCTGTAGCAGGCGGACCATCGTCAG
GAAGCGGTATTGCACCGTCGGCAAGCGGACCATCTGGAAGCGCTATTGCACCGGCGGCAGACGGACCAACAGTAAGTAT
GTCACCGGGACCATCGGCAGGAGGACCATTGGCAGGCGGACCATCGGAGGGAGCACCGGGAAGTAGTGCTTTAGGTCC
CGGCGGCAGCAATACTCCGGCGGACATCAATACGCCAGCTGGCGCACCAGAAAAC
>gi|73808537|gb|DQ103392.1| Lycopersicon pimpinellifolium CT099 (CT099) gene, CT099-7276a allele,
partial cds
CTCAGCATATGAGATTTATAATCAACGACTCTGTTCGTAAGTCTTAACATGTCTCTCTTTATTTATTATTGCTTGAAGAGTTT
GAACAATATAATTGGTAAACGAGTTTTTTCTATATGTTGATACTTTTTTTTTTGTGTGTGTGCGCTTTTTTTAGTGTTTAAGT
ATAAGCAAGGAGCAGATTCTGTGTTGGAGGTGAGTAAGGATGATTATGATAAGTGCAATACTGGAAATCCAATTAAGAA
AATGGAGGATGGAAACTCGATTTTCACGTTGGATCGGTCTGGTCCTTTTTACTTTATCAGTGGGAACAAAGATAACTGTGA
TAAAGGCCAAAAGTTGCAAATTGTTGTGATTTCTGCTAGGAATCAAGGTAAACCACCACAAACACCGGCACCGGCAGTTG
CTCCACCTAGTAATGGATCAACTACACCATCTACTCCTTCTGGAGGTACGCGGAATCAGGATTTTTATTTTATTCCCGTTCA
ATTTTACGTTGACATCTCCTTACGTAGTTTGCGAGTTATTGTATCTAAGCATTGAGAACTTACGTGATTAATGCAGGCTCAA
CTCCGGCAGCTGCTCCGTCTAAAGGATCGGGTACACCGTCGGCACCTTCAGCTAACGCTCCGGCGGGATCATCTAAACCG
GGTGCATCGTCACCTAATGGGGCACCGGTATCTACTCCTGCAGGTAAATGTAGAATTTCGATTTGATGGATTCAACGTTTA
ATTTCGTTCGATTTTTAACGTATTGAACGTAAATATGAATCATATAGTTTATCTGTTTGTTCAGGTAAATCACCAACCTCATC
ACCTACACCTTCCGGTTCAACCGCATCTCCACCATCTCCTGCGACCGTAACTCCGGCAATGTCTCCGGTAGCAAATGGACC
GTCAACTAGTACACCAGGTTCTTCTTCACCTGTAGCAGGCGGACCATCGTCAGGAAGCGGTATTGCACCGTCGGCAAGCG
GACCATCTGGAAGCGCTATTGCACCGGCGGCAGACGGACCAACAGTAAGTATGTCACCGGGACCATCGGCAGGAGGACC
ATTGGCAGGCGGACCATCGGAGGGAGCACCGGGAAGTAGTGCTTTAGGTCCCGGCGGCAGCAATACTCCGGCGGACAT
CAATACGCCAGCTGGCGCACCAGAAAACCCAAATTCATTTGCAGTGAAGGCATT
PRACTICAS DE BIOINFORMATICA E.P. BIOLOGIA UNSA
>gi|73808535|gb|DQ103391.1| Lycopersicon pimpinellifolium CT099 (CT099) gene, CT099-7275b allele,
partial cds
TACAACACTTGGTCTCAGCATATGAGATTTATAATCAACGACTCTGTTCGTAAGTCTTAACATGTCTCTCTTTATTTATTATT
GCTTGAAGAGTTTGAACAATATAATTGGTAAACGAGTTTTTTCTATATGTTGATACTTTTTTTTTTGTGTGTGTGCGCTTTTT
TTAGTGTTTAAGTATAAGCAAGGAGCAGATTCTGTGTTGGAGGTGAGTAAGGATGATTATGATAAGTGCAATACTGGAAA
TCCAATTAAGAAAATGGAGGATGGAAACTCGATTTTCACGTTGGATCGGTCTGGTCCTTTTTACTTTATCAGTGGGAACAA
AGATAACTGTGATAAAGGCCAAAAGTTGCAAATTGTTGTGATTTCTGCTAGGAATCAAGGTAAACCACCACAAACACCGG
CACCGGCAGTTGCTCCACCTAGTAATGGATCAACTACACCATCTACTCCTTCTGGAGGTACGCGGAATCAGGATTTTTATTT
TATTCCCGTTCAATTTTACGTTGACATCTCCTTACGTAGTTTGCGAGTTATTGTATCTAAGGATAAAAATTACATGATTCGAT
TGAGAACTTACGTGATTAATGCAGGCTCAACTCCGGCAGCTGCTCCGTCTAAAGGATCGGGTACACCGTCGGCACCTTCA
GCTAACGCTCCGGCGGGATCATCTAAACCGGGTGCATCGTCACCTAATGGGGCACCGGTATCTACTCCTGCAGGTAAATG
TAGAATTTCGATTTGATGGATTCAACGTTTAATTTCGTTCGATTTTTAACGTATTGAACGTAAATTGAATCATATAGTTTATC
TGTTTGTTCAGGTAAATCACCAACCTCATCACCTACACCTTCCGGTTCAACCGCATCTCCACCATCTCCTGCGACCGTAACTC
CGGCAATGTCTCCGGTAGCAAATGGACCGTCAACTAGTACACCAGGTTCTTCTTCACCTGTAGCAGGCGGACCATCGTCA
GGAAGCGGTATTGCACCGTCGGCAGGCGGACCATCTGGAAGCGCTATTGCACCGGCGGCAGACGGACCAACAGTAAGT
ATGTCACCGGGACCATCGGCAGGAGGACCATTGGCAGGCGGACCATCGGAGGGAGCACCGGGAAGTAGTGCTTTAGGT
CCCGGCGGCAGCAATACTCCGGCGGACATCAATACGCCAGCTGGCGCACCAGAAAACCCAAATTCATTTGCAGTGAAG
>gi|73808533|gb|DQ103390.1| Lycopersicon pimpinellifolium CT099 (CT099) gene, CT099-7275a allele,
partial cds
CTTAACCCTTCTGTTGATTACAACACTTGGTCTCAGCATATGAGATTTATAATCAACGACTCTGTTCGTAAGTCTTAACATGT
CTCTCTTTATTTATTATTGCTTGAAGAGTTTGAACAATATAATTGGTAAACGAGTTTTTTCTATATGTTGATACTTTTTTTTTT
GTGTGTGCGCGCTTTTTTTAGTGTTTAAGTATAAGCAAGGAGCAGATTCTGTGTTGGAGGTGAGTAAGGATGATTATGAT
AAGTGCAATACTGGAAATCCAATTAAGAAAATGGAGGATGGAAACTCGATTTTCACGTTGGATCGGTCTGGTCCTTTTTAC
TTTATCAGTGGGAACAAAGATAACTGTGATAAAGGCCAAAAGTTGCAAATTGTTGTGATTTCTGCTAGGAATCAAGGTAA
ACCACCACAAACACCGGCACCGGCAGTTGCTCCACCTAGTAATGGATCAACTACACCATCTACTCCTTCTGGAGGTACGCG
GAATCAGGATTTTTATTTTATTCCCGTTCAATTTTACGTTGACATCTCCTTATGTGGTTTGCGAGTTATTGTATCTAAGCATT
GAGAACTTACGTGATTAATGCAGGCTCAACTCCGGCAGCTGCTCCGTCTAAAGGATCGGGTACACCGTCGGCACCTTCAG
CTAACGCTCCGGCGGGATCATCTAAACCGGGTGCATCGTCACCTAATGGGGCACCGGTATCTACTCCTGCAGGTAAATGT
AGAATTTCGATTTGATGGATTCAACGTTTAATTTCGTTCGATTTTTAACGTATTGAACGTAAATTGAATCATATAGTTTATCT
GTTTGTTCAGGTAAATCACCAACCTCATCACCTACACCTTCCGGTTCAACCGCATCTCCACCATCTCCTGCGACCGTAACTCC
GGCAATGTCTCCGGTAGCAAATGGACCGTCAACTAGTACACCAGGTTCTTCTTCACCTGTAGCAGGCGGACCATCGTCAG
GAAGCGGTATTGCACCGTCGGCAAGCGGACCATCTGGAAGCGCTATTGCACCGGCGGCAGACGGACCAACAGTAAGTAT
GTCACCGGGACCATCGGCAGGAGGACCATTGGCAGGCGGACCATCGGAGGGAGCACCGGGAAGTAGTGCTTTAGGTCC
CGGCGGCAGCAATACTCCGGCGGACATCAATACGCCAGCTGGCGCACCAGAAAACCCAAATTCATTTGCAGTGAAGGCAT
TTACTCCATCAGTTGTGCTTGTG
4. Hacer el alineamiento múltiple de la secuencias del gen CT09
de Lycopersicon pimpinellifolium (10 muestras) , utilizando BioEdit, el la opción
“accessory Apliccation” escoger “ClustalW Multiple Aligment”
LOCUS gi|73808543|gb|DQ103395.1| Lycopersicon pimpinellifolium CT099
(CT099) gene, CT099-7277b allele, partial cds 1260 bp
DEFINITION gi|73808543|gb|DQ103395.1| Lycopersicon pimpinellifolium CT099
(CT099) gene, CT099-7277b allele, partial cds 1260 bp
TITLE gi|73808543|gb|DQ103395.1| Lycopersicon pimpinellifolium CT099
(CT099) gene, CT099-7277b allele, partial cds
ORIGIN
1 ----ACCCTT CTGTTGATTA CAACACTTGG TCTCAGCATA TGAGATTTAT AATCAACGAC
61 TCTGTTCGTA AGTCTTAACA TGTCTCTCTT TATTTATTAT TGCTTGAAGA GTTTGAACAA
121 TATAATTGGT AAACGAGTTT TTTCTATA-T GTTGATACTT TTTTTTTTGT GTGTGTGCGC
181 TTTTTTTAGT GTTTAAGTAT AAGCAAGGAG CAGATTCTGT GTTGGAGGTG AGTAAGGATG
PRACTICAS DE BIOINFORMATICA E.P. BIOLOGIA UNSA
241 ATTATGATAA GTGCAATACT GGAAATCCAA TTAAGAAAAT GGAGGATGGA AACTCGATTT
301 TCACGTTGGA TCGGTCTGGT CCTTTTTACT TTATCAGTGG GAACAAAGAT AACTGTGATA
361 AAGGCCAAAA GTTGCAAATT GTTGTGATTT CTGCTAGGAA TCAAGGTAAA CCACCACAAA
421 CACCGGCACC GGCAGTTGCT CCACCTAGTA ATGGATCAAC TACACCATCT ACTCCTTCTG
481 GAGGTACGCG GAATCAGGAT TTTTATTTTA TTCCCGTTGC AATTTTACGT TGACATCTCC
541 TTACGTAGTT TGCGAGTTAT TGTATCTAAG GAT------- ---------- --TGAGAACT
601 TACGTGATTA ATGCAGGCTC AACTCCGGCA GCTGCTCCGT CTAAAGGATC GGGTACACCG
661 TCGGCACCTT CAGCTAACGC TCCGGCGGGA TCATCTAAAC CGGGTGCATC GTCACCTAAT
721 GGGGCACCGG TATCTACTCC TGCAGGTAAA TGTAGAATTT CGATTTGATG GATTCAACGT
781 TTAATTTCGT TCGATTTTTA ACGTATTGAA CGTAAAT-TG AATCATATAG TTTATCTGTT
841 TGTTCAGGTA AATCACCAAC CTCATCACCT ACACCTTCCG GTTCAACCGC ATCTCCACCA
901 TCTCCTGCGA CCGTAACTCC GGCAATGTCT CCGGTAGCAA ATGGACCGTC AACTAGTACA
961 CCAGGTTCTT CTTCACCTGT AGCAGGCGGA CCATCGTCAG GAAGCGGTAT TGCACCGTCG
1021 GCAAGCGGAC CATCTGGAAG CGCTATTGCA CCGGCGGCAG ACGGACCAAC
AGTAAGTATG
1081 TCACCGGGAC CATCGGCAGG AGGACCATTG GCAGGCGGAC CATCGGAGGG
AGCACCGGGA
1141 AGTAGTGCTT TAGGTCCCGG CGGCAGCAAT ACTCCGGCGG ACATCAATAC
GCCAGCTGGC
1201 GCACCAGAAA ACCCAAATTC ATTTGCAGTG AAGGCATTTA CTCCATCAGT
TGTGCTTGTG
//
LOCUS gi|73808541|gb|DQ103394.1| Lycopersicon pimpinellifolium CT099
(CT099) gene, CT099-7277a allele, partial cds 1260 bp
DEFINITION gi|73808541|gb|DQ103394.1| Lycopersicon pimpinellifolium CT099
(CT099) gene, CT099-7277a allele, partial cds 1260 bp
TITLE gi|73808541|gb|DQ103394.1| Lycopersicon pimpinellifolium CT099
(CT099) gene, CT099-7277a allele, partial cds
ORIGIN
1 ---------- ---------- --------GG TCTCAGCATA TGAGATTTAT AATCAACGAC
61 TCTGTTCGTA AGTCTTAACA TGTCTCTCTT TATTTATTAT TGCTTGAAGA GTTTGAACAA
121 TATAATTGGT AAACGAGTTT TTTCTATA-T GTTGATACTT TTTTTTTTGT GTGTGTGCGC
181 TTTTTTTAGT GTTTAAGTAT AAGCAAGGAG CAGATTCTGT GTTGGAGGTG AGTAAGGATG
241 ATTATGATAA GTGCAATACT GGAAATCCAA TTAAGAAAAT GGAGGATGGA AACTCGATTT
301 TCACGTTGGA TCGGTCTGGT CCTTTTTACT TTATCAGTGG GAACAAAGAT AACTGTGATA
361 AAGGCCAAAA GTTGCAAATT GTTGTGATTT CTGCTAGGAA TCAAGGTAAA CCACCACAAA
421 CACCGGCACC GGCAGTTGCT CCACCTAGTA ATGGATCAAC TACACCATCT ACTCCTTCTG
481 GAGGTACGCG GAATCAGGAT TTTTATTTTA TTCCCGTT-C AATTTTACGT TGACATCTCC
541 TTACGTAGTT TGCGAGTTAT TGTATCTAAG GAT------- ---------- --TGAGAACT
PRACTICAS DE BIOINFORMATICA E.P. BIOLOGIA UNSA
601 TACGTGATTA ATGCAGGCTC AACTCCGGCA GCTGCTCCGT CTAAAGGATC GGGTACACCG
661 TCGGCACCTT CAGCTAACGC TCCGGCGGGA TCATCTAAAC CGGGTGCATC GTCACCTAAT
721 GGGGCACCGG TATCTACTCC TGCAGGTAAA TGTAGAATTT CGATTTGATG GATTCAACGT
781 TTAATTTCGT TCGATTTTTA ACGTATTGAA CGTAAAT-TG AATCATATAG TTTATCTGTT
841 TGTTCAGGTA AATCACCAAC CTCATCACCT ACACCTTCCG GTTCAACCGC ATCTCCACCA
901 TCTCCTGCGA CCGTAACTCC GGCAATGTCT CCGGTAGCAA ATGGACCGTC AACTAGTACA
961 CCAGGTTCTT CTTCACCTGT AGCAGGCGGA CCATCGTCAG GAAGCGGTAT TGCACCGTCG
1021 GCAAGCGGAC CATCTGGAAG CGCTATTGCA CCGGCGGCAG ACGGACCAAC
AGTAAGTATG
1081 TCACCGGGAC CATCGGCAGG AGGACCATTG GCAGGCGGAC CATCGGAGGG
AGCACCGGGA
1141 AGTAGTGCTT TAGGTCCCGG CGGCAGCAAT ACTCCGGCGG ACATCAATAC
GCCAGCTGGC
1201 GCACCAGAAA ACCCAAATTC ATTTGCAGTG AAGGCATTTA CTCCATCAGT
TGTGCTTGTG
//
LOCUS gi|73808539|gb|DQ103393.1| Lycopersicon pimpinellifolium CT099
(CT099) gene, CT099-7276b allele, partial cds 1260 bp
DEFINITION gi|73808539|gb|DQ103393.1| Lycopersicon pimpinellifolium CT099
(CT099) gene, CT099-7276b allele, partial cds 1260 bp
TITLE gi|73808539|gb|DQ103393.1| Lycopersicon pimpinellifolium CT099
(CT099) gene, CT099-7276b allele, partial cds
ORIGIN
1 CTTAACCCTT CTGTTGATTA CAACACTTGG TCTCAGCATA TGAGATTTAT AATCAACGAC
61 TCTGTTCGTA AGTCTTAACA TGTCTCTCTT TATTTATTAT TGCTTGAAGA GTTTGAACAA
121 TATAATTGGT AAACGAGTTT TTTCTATA-T GTTGATACTT TTTTTTTTGT GTGTGTGCGC
181 TTTTTTTAGT GTTTAAGTAT AAGCAAGGAG CAGATTCTGT GTTGGAGGTG AGTAAGGATG
241 ATTATGATAA GTGCAATACT GGAAATCCAA TTAAGAAAAT GGAGGATGGA AACTCGATTT
301 TCACGTTGGA TCGGTCTGGT CCTTTTTACT TTATCAGTGG GAACAAAGAT AACTGTGATA
361 AAGGCCAAAA GTTGCAAATT GTTGTGATTT CTGCTAGGAA TCAAGGTAAA CCACCACAAA
421 CACCGGCACC GGCAGTTGCT CCACCTAGTA ATGGATCAAC TACACCATCT ACTCCTTCTG
481 GAGGTACGCG GAATCAGGAT TTTTATTTTA TTCCCGTT-C AATTTTACGT TGACATCTCC
541 TTACGTAGTT TGCGAGTTAT TGTATCTAAG CAT------- ---------- --TGAGAACT
601 TACGTGATTA ATGCAGGCTC AACTCCGGCA GCTGCTCCGT CTAAAGGATC GGGTACACCG
661 TCGGCACCTT CAGCTAACGC TCCGGCGGGA TCATCTAAAC CGGGTGCATC GTCACCTAAT
721 GGGGCACCGG TATCTACTCC TGCAGGTAAA TGTAGAATTT CGATTTGATG GATTCAACGT
781 TTAATTTCGT TCGATTTTTA ACGTATTGAA CGTAAAT-TG AATCATATAG TTTATCTGTT
841 TGTTCAGGTA AATCACCAAC CTCATCACCT ACACCTTCCG GTTCAACCGC ATCTCCACCA
901 TCTCCTGCGA CCGTAACTCC GGCAATGTCT CCGGTAGCAA ATGGACCGTC AACTAGTACA
PRACTICAS DE BIOINFORMATICA E.P. BIOLOGIA UNSA
961 CCAGGTTCTT CTTCACCTGT AGCAGGCGGA CCATCGTCAG GAAGCGGTAT TGCACCGTCG
1021 GCAAGCGGAC CATCTGGAAG CGCTATTGCA CCGGCGGCAG ACGGACCAAC
AGTAAGTATG
1081 TCACCGGGAC CATCGGCAGG AGGACCATTG GCAGGCGGAC CATCGGAGGG
AGCACCGGGA
1141 AGTAGTGCTT TAGGTCCCGG CGGCAGCAAT ACTCCGGCGG ACATCAATAC
GCCAGCTGGC
1201 GCACCAGAAA AC-------- ---------- ---------- ---------- --------
--
//
LOCUS gi|73808537|gb|DQ103392.1| Lycopersicon pimpinellifolium CT099
(CT099) gene, CT099-7276a allele, partial cds 1260 bp
DEFINITION gi|73808537|gb|DQ103392.1| Lycopersicon pimpinellifolium CT099
(CT099) gene, CT099-7276a allele, partial cds 1260 bp
TITLE gi|73808537|gb|DQ103392.1| Lycopersicon pimpinellifolium CT099
(CT099) gene, CT099-7276a allele, partial cds
ORIGIN
1 ---------- ---------- ---------- -CTCAGCATA TGAGATTTAT AATCAACGAC
61 TCTGTTCGTA AGTCTTAACA TGTCTCTCTT TATTTATTAT TGCTTGAAGA GTTTGAACAA
121 TATAATTGGT AAACGAGTTT TTTCTATA-T GTTGATACTT TTTTTTTTGT GTGTGTGCGC
181 TTTTTTTAGT GTTTAAGTAT AAGCAAGGAG CAGATTCTGT GTTGGAGGTG AGTAAGGATG
241 ATTATGATAA GTGCAATACT GGAAATCCAA TTAAGAAAAT GGAGGATGGA AACTCGATTT
301 TCACGTTGGA TCGGTCTGGT CCTTTTTACT TTATCAGTGG GAACAAAGAT AACTGTGATA
361 AAGGCCAAAA GTTGCAAATT GTTGTGATTT CTGCTAGGAA TCAAGGTAAA CCACCACAAA
421 CACCGGCACC GGCAGTTGCT CCACCTAGTA ATGGATCAAC TACACCATCT ACTCCTTCTG
481 GAGGTACGCG GAATCAGGAT TTTTATTTTA TTCCCGTT-C AATTTTACGT TGACATCTCC
541 TTACGTAGTT TGCGAGTTAT TGTATCTAAG CAT------- ---------- --TGAGAACT
601 TACGTGATTA ATGCAGGCTC AACTCCGGCA GCTGCTCCGT CTAAAGGATC GGGTACACCG
661 TCGGCACCTT CAGCTAACGC TCCGGCGGGA TCATCTAAAC CGGGTGCATC GTCACCTAAT
721 GGGGCACCGG TATCTACTCC TGCAGGTAAA TGTAGAATTT CGATTTGATG GATTCAACGT
781 TTAATTTCGT TCGATTTTTA ACGTATTGAA CGTAAATATG AATCATATAG TTTATCTGTT
841 TGTTCAGGTA AATCACCAAC CTCATCACCT ACACCTTCCG GTTCAACCGC ATCTCCACCA
901 TCTCCTGCGA CCGTAACTCC GGCAATGTCT CCGGTAGCAA ATGGACCGTC AACTAGTACA
961 CCAGGTTCTT CTTCACCTGT AGCAGGCGGA CCATCGTCAG GAAGCGGTAT TGCACCGTCG
1021 GCAAGCGGAC CATCTGGAAG CGCTATTGCA CCGGCGGCAG ACGGACCAAC
AGTAAGTATG
1081 TCACCGGGAC CATCGGCAGG AGGACCATTG GCAGGCGGAC CATCGGAGGG
AGCACCGGGA
1141 AGTAGTGCTT TAGGTCCCGG CGGCAGCAAT ACTCCGGCGG ACATCAATAC
GCCAGCTGGC
PRACTICAS DE BIOINFORMATICA E.P. BIOLOGIA UNSA
1201 GCACCAGAAA ACCCAAATTC ATTTGCAGTG AAGGCATT-- ---------- --------
--
//
LOCUS gi|73808535|gb|DQ103391.1| Lycopersicon pimpinellifolium CT099
(CT099) gene, CT099-7275b allele, partial cds 1260 bp
DEFINITION gi|73808535|gb|DQ103391.1| Lycopersicon pimpinellifolium CT099
(CT099) gene, CT099-7275b allele, partial cds 1260 bp
TITLE gi|73808535|gb|DQ103391.1| Lycopersicon pimpinellifolium CT099
(CT099) gene, CT099-7275b allele, partial cds
ORIGIN
1 ---------- --------TA CAACACTTGG TCTCAGCATA TGAGATTTAT AATCAACGAC
61 TCTGTTCGTA AGTCTTAACA TGTCTCTCTT TATTTATTAT TGCTTGAAGA GTTTGAACAA
121 TATAATTGGT AAACGAGTTT TTTCTATA-T GTTGATACTT TTTTTTTTGT GTGTGTGCGC
181 TTTTTTTAGT GTTTAAGTAT AAGCAAGGAG CAGATTCTGT GTTGGAGGTG AGTAAGGATG
241 ATTATGATAA GTGCAATACT GGAAATCCAA TTAAGAAAAT GGAGGATGGA AACTCGATTT
301 TCACGTTGGA TCGGTCTGGT CCTTTTTACT TTATCAGTGG GAACAAAGAT AACTGTGATA
361 AAGGCCAAAA GTTGCAAATT GTTGTGATTT CTGCTAGGAA TCAAGGTAAA CCACCACAAA
421 CACCGGCACC GGCAGTTGCT CCACCTAGTA ATGGATCAAC TACACCATCT ACTCCTTCTG
481 GAGGTACGCG GAATCAGGAT TTTTATTTTA TTCCCGTT-C AATTTTACGT TGACATCTCC
541 TTACGTAGTT TGCGAGTTAT TGTATCTAAG GATAAAAATT ACATGATTCG ATTGAGAACT
601 TACGTGATTA ATGCAGGCTC AACTCCGGCA GCTGCTCCGT CTAAAGGATC GGGTACACCG
661 TCGGCACCTT CAGCTAACGC TCCGGCGGGA TCATCTAAAC CGGGTGCATC GTCACCTAAT
721 GGGGCACCGG TATCTACTCC TGCAGGTAAA TGTAGAATTT CGATTTGATG GATTCAACGT
781 TTAATTTCGT TCGATTTTTA ACGTATTGAA CGTAAAT-TG AATCATATAG TTTATCTGTT
841 TGTTCAGGTA AATCACCAAC CTCATCACCT ACACCTTCCG GTTCAACCGC ATCTCCACCA
901 TCTCCTGCGA CCGTAACTCC GGCAATGTCT CCGGTAGCAA ATGGACCGTC AACTAGTACA
961 CCAGGTTCTT CTTCACCTGT AGCAGGCGGA CCATCGTCAG GAAGCGGTAT TGCACCGTCG
1021 GCAGGCGGAC CATCTGGAAG CGCTATTGCA CCGGCGGCAG ACGGACCAAC
AGTAAGTATG
1081 TCACCGGGAC CATCGGCAGG AGGACCATTG GCAGGCGGAC CATCGGAGGG
AGCACCGGGA
1141 AGTAGTGCTT TAGGTCCCGG CGGCAGCAAT ACTCCGGCGG ACATCAATAC
GCCAGCTGGC
1201 GCACCAGAAA ACCCAAATTC ATTTGCAGTG AAG------- ---------- --------
--
//
LOCUS gi|73808533|gb|DQ103390.1| Lycopersicon pimpinellifolium CT099
(CT099) gene, CT099-7275a allele, partial cds 1260 bp
DEFINITION gi|73808533|gb|DQ103390.1| Lycopersicon pimpinellifolium CT099
(CT099) gene, CT099-7275a allele, partial cds 1260 bp
PRACTICAS DE BIOINFORMATICA E.P. BIOLOGIA UNSA
TITLE gi|73808533|gb|DQ103390.1| Lycopersicon pimpinellifolium CT099
(CT099) gene, CT099-7275a allele, partial cds
ORIGIN
1 CTTAACCCTT CTGTTGATTA CAACACTTGG TCTCAGCATA TGAGATTTAT AATCAACGAC
61 TCTGTTCGTA AGTCTTAACA TGTCTCTCTT TATTTATTAT TGCTTGAAGA GTTTGAACAA
121 TATAATTGGT AAACGAGTTT TTTCTATA-T GTTGATACTT TTTTTTTTGT GTGTGCGCGC
181 TTTTTTTAGT GTTTAAGTAT AAGCAAGGAG CAGATTCTGT GTTGGAGGTG AGTAAGGATG
241 ATTATGATAA GTGCAATACT GGAAATCCAA TTAAGAAAAT GGAGGATGGA AACTCGATTT
301 TCACGTTGGA TCGGTCTGGT CCTTTTTACT TTATCAGTGG GAACAAAGAT AACTGTGATA
361 AAGGCCAAAA GTTGCAAATT GTTGTGATTT CTGCTAGGAA TCAAGGTAAA CCACCACAAA
421 CACCGGCACC GGCAGTTGCT CCACCTAGTA ATGGATCAAC TACACCATCT ACTCCTTCTG
481 GAGGTACGCG GAATCAGGAT TTTTATTTTA TTCCCGTT-C AATTTTACGT TGACATCTCC
541 TTATGTGGTT TGCGAGTTAT TGTATCTAAG CAT------- ---------- --TGAGAACT
601 TACGTGATTA ATGCAGGCTC AACTCCGGCA GCTGCTCCGT CTAAAGGATC GGGTACACCG
661 TCGGCACCTT CAGCTAACGC TCCGGCGGGA TCATCTAAAC CGGGTGCATC GTCACCTAAT
721 GGGGCACCGG TATCTACTCC TGCAGGTAAA TGTAGAATTT CGATTTGATG GATTCAACGT
781 TTAATTTCGT TCGATTTTTA ACGTATTGAA CGTAAAT-TG AATCATATAG TTTATCTGTT
841 TGTTCAGGTA AATCACCAAC CTCATCACCT ACACCTTCCG GTTCAACCGC ATCTCCACCA
901 TCTCCTGCGA CCGTAACTCC GGCAATGTCT CCGGTAGCAA ATGGACCGTC AACTAGTACA
961 CCAGGTTCTT CTTCACCTGT AGCAGGCGGA CCATCGTCAG GAAGCGGTAT TGCACCGTCG
1021 GCAAGCGGAC CATCTGGAAG CGCTATTGCA CCGGCGGCAG ACGGACCAAC
AGTAAGTATG
1081 TCACCGGGAC CATCGGCAGG AGGACCATTG GCAGGCGGAC CATCGGAGGG
AGCACCGGGA
1141 AGTAGTGCTT TAGGTCCCGG CGGCAGCAAT ACTCCGGCGG ACATCAATAC
GCCAGCTGGC
1201 GCACCAGAAA ACCCAAATTC ATTTGCAGTG AAGGCATTTA CTCCATCAGT
TGTGCTTGTG
//
LOCUS gi|73808531|gb|DQ103389.1| Lycopersicon pimpinellifolium CT099
(CT099) gene, CT099-7274b allele, partial cds 1260 bp
DEFINITION gi|73808531|gb|DQ103389.1| Lycopersicon pimpinellifolium CT099
(CT099) gene, CT099-7274b allele, partial cds 1260 bp
TITLE gi|73808531|gb|DQ103389.1| Lycopersicon pimpinellifolium CT099
(CT099) gene, CT099-7274b allele, partial cds
ORIGIN
1 ---------- ---------- ---CACTTGG TCTCAGCATA TGAGATTTAT AATCAACGAC
61 TCTGTTCGTA AGTCTTAACA TGTCTCTCTT TATTTATTAT TGCTTGAAGA GTTTGAACAA
PRACTICAS DE BIOINFORMATICA E.P. BIOLOGIA UNSA
121 TATAATTGGT AAACGAGTTT TTTCTATA-T GTTGATACTT TTTTTTTTGT GTGTGCGCGC
181 TTTTTTTAGT GTTTAAGTAT AAGCAAGGAG CAGATTCTGT GTTGGAGGTG AGTAAGGATG
241 ATTATGATAA GTGCAATACT GGAAATCCAA TTAAGAAAAT GGAGGATGGA AACTCGATTT
301 TCACGTTGGA TCGGTCTGGT CCTTTTTACT TTATCAGTGG GAACAAAGAT AACTGTGATA
361 AAGGCCAAAA GTTGCAAATT GTTGTGATTT CTGCTAGGAA TCAAGGTAAA CCACCACAAA
421 CACCGGCACC GGCAGTTGCT CCACCTAGTA ATGGATCAAC TACACCATCT ACTCCTTCTG
481 GAGGTACGCG GAATCAGGAT TTTTATTTTA TTCCCGTT-C AATTTTACGT TGACATCTCC
541 TTATGTGGTT TGCGAGTTAT TGTATCTAAG GATAAAAATT ACATGATTCG ATTGAGAACT
601 TACGTGATTA ATGCAGGCTC AACTCCGGCA GCTGCTCCGT CTAAAGGATC GGGTACACCG
661 TCGGCACCTT CAGCTAACGC TCCGGCGGGA TCATCTAAAC CGGGTGCATC GTCACCTAAT
721 GGGGCACCGG TATCTACTCC TGCAGGTAAA TGTAGAATTT CGATTTGATG GATTCAACGT
781 TTAATTTCGT TCGATTTTTA ACGTATTGAA CGTAAAT-TG AATCATATAG TTTATCTGTT
841 TGTTCAGGTA AATCACCAAC CTCATCACCT ACACCTTCCG GTTCAACCGC ATCTCCACCA
901 TCTCCTGCGA CCGTAACTCC GGCAATGTCT CCGGTAGCAA ATGGACCGTC AACTAGTACA
961 CCAGGTTCTT CTTCACCTGT AGCAGGCGGA CCATCGTCAG GAAGCGGTAT TGCACCGTCG
1021 GCAGGCGGAC CATCTGGAAG CGCTATTGCA CCGGCGGCAG ACGGACCAAC
AGTAAGTATG
1081 TCACCGGGAC CATCGGCAGG AGGACCATTG GCAGGCGGAC CATCGGAGGG
AGCACCGGGA
1141 AGTAGTGCTT TAGGTCCCGG CGGCAGCAAT ACTCCGGCGG ACATCAATAC
GCCAGCTGGC
1201 GCACCAGAAA ACCCAAATTC ATTTGCAGTG ---------- ---------- --------
--
//
LOCUS gi|73808529|gb|DQ103388.1| Lycopersicon pimpinellifolium CT099
(CT099) gene, CT099-7274a allele, partial cds 1260 bp
DEFINITION gi|73808529|gb|DQ103388.1| Lycopersicon pimpinellifolium CT099
(CT099) gene, CT099-7274a allele, partial cds 1260 bp
TITLE gi|73808529|gb|DQ103388.1| Lycopersicon pimpinellifolium CT099
(CT099) gene, CT099-7274a allele, partial cds
ORIGIN
1 ----ACCCTT CTGTTGATTA CAACACTTGG TCTCAGCATA TGAGATTTAT AATCAACGAC
61 TCTGTTCGTA AGTCTTAACA TGTCTCTCTT TATTTATTAT TGCTTGAAGA GTTTGAACAA
121 TATAATTGGT AAACGAGTTT TTTCTATA-T GTTGATACTT TTTTTTTTGT GTGTGCGCGC
181 TTTTTTTAGT GTTTAAGTAT AAGCAAGGAG CAGATTCTGT GTTGGAGGTG AGTAAGGATG
241 ATTATGATAA GTGCAATACT GGAAATCCAA TTAAGAAAAT GGAGGATGGA AACTCGATTT
301 TCACGTTGGA TCGGTCTGGT CCTTTTTACT TTATCAGTGG GAACAAAGAT AACTGTGATA
361 AAGGCCAAAA GTTGCAAATT GTTGTGATTT CTGCTAGGAA TCAAGGTAAA CCACCACAAA
PRACTICAS DE BIOINFORMATICA E.P. BIOLOGIA UNSA
421 CACCGGCACC GGCAGTTGCT CCACCTAGTA ATGGATCAAC TACACCATCT ACTCCTTCTG
481 GAGGTACGCG GAATCAGGAT TTTTATTTTA TTCCCGTT-C AATTTTACGT TGACATCTCC
541 TTATGTGGTT TGCGAGTTAT TGTATCTAAG GATAAAAATT ACATGATTCG ATTGAGAACT
601 TACGTGATTA ATGCAGGCTC AACTCCGGCA GCTGCTCCGT CTAAAGGATC GGGTACACCG
661 TCGGCACCTT CAGCTAACGC TCCGGCGGGA TCATCTAAAC CGGGTGCATC GTCACCTAAT
721 GGGGCACCGG TATCTACTCC TGCAGGTAAA TGTAGAATTT CGATTTGATG GATTCAACGT
781 TTAATTTCGT TCGATTTTTA ACGTATTGAA CGTAAAT-TG AATCATATAG TTTATCTGTT
841 TGTTCAGGTA AATCACCAAC CTCATCACCT ACACCTTCCG GTTCAACCGC ATCTCCACCA
901 TCTCCTGCGA CCGTAACTCC GGCAATGTCT CCGGTAGCAA ATGGACCGTC AACTAGTACA
961 CCAGGTTCTT CTTCACCTGT AGCAGGCGGA CCATCGTCAG GAAGCGGTAT TGCACCGTCG
1021 GCAGGCGGAC CATCTGGAAG CGCTATTGCA CCGGCGGCAG ACGGACCAAC
AGTAAGTATG
1081 TCACCGGGAC CATCGGCAGG AGGACCATTG GCAGGCGGAC CATCGGAGGG
AGCACCGGGA
1141 AGTAGTGCTT TAGGTCCCGG CGGCAGCAAT ACTCCGGCGG ACATCAATAC
GCCAGCTGGC
1201 GCACCAGAAA ACCCAAATTC ATTTGCAGTG AAGGCATTTA CTCCATCAGT
TGTACTTGTG
//
LOCUS gi|73808527|gb|DQ103387.1| Lycopersicon pimpinellifolium CT099
(CT099) gene, CT099-7272b allele, partial cds 1260 bp
DEFINITION gi|73808527|gb|DQ103387.1| Lycopersicon pimpinellifolium CT099
(CT099) gene, CT099-7272b allele, partial cds 1260 bp
TITLE gi|73808527|gb|DQ103387.1| Lycopersicon pimpinellifolium CT099
(CT099) gene, CT099-7272b allele, partial cds
ORIGIN
1 ---------- ---------- CAACACTTGG TCTCAGCATA TGAGATTTAT AATCAACGAC
61 TCTGTTCGTA AGTCTTAACA TGTCTCTCTT TATTTATTAT TGCTTGAAGA GATCGAACAA
121 TATAATTGGT AAACGAGTTT TTTCTATA-T GTTGATACTT TTTTTTTTGT GTGTGCGCGC
181 TTTTTTTAGT GTTTAAGTAT AAGCAAGGAG CAGATTCTGT GTTGGAGGTG AGTAAGGATG
241 ATTATGATAA GTGCAATACT GGAAATCCAA TTAAGAAAAT GGAGGATGGA AACTCGATTT
301 TCACGTTGGA TCGGTCTGGT CCTTTTTACT TTATCAGTGG GAACAAAGAT AACTGTGATA
361 AAGGCCAAAA GTTGCAAATT GTTGTGATTT CTGCTAGGAA TCAAGGTAAA CCACCACAAA
421 CACCGGCACC GGCAGTTGCT CCACCTAGTA ATGGATCAAC TACACCATCT ACTCCTTCTG
481 GAGGTACGCG GAATCAGGAT TTTTATTTTA TTCCCGTT-C AATTTTACGT TGACATCTCC
541 TTATGTGGTT TGCGAGTTAT TGTATCTAAG GATAAAAATT ACATGATTCG ATTGAGAACT
601 TACGTGATTA ATGCAGGCTC AACTCCGGCA GCTGCTCCGT CTAAAGGATC GGGTACACCG
661 TCGGCACCTT CAGCTAACGC TCCGGCGGGA TCATCTAAAC CGGGTGCATC GTCACCTAAT
721 GGGGCACCGG TATCTACTCC TGCAGGTAAA TGTAGAATTT CGATTTGATG GATTCAACGT
PRACTICAS DE BIOINFORMATICA E.P. BIOLOGIA UNSA
781 TTAATTTCGT TCGATTTTTA ACGTATTGAA CGTAAAT-TG AATCATATAG TTTATCTGTT
841 TGTTCAGGTA AATCACCAAC CTCATCACCT ACACCTTCCG GTTCAACCGC ATCTCCACCA
901 TCTCCTGCGA CCGTAACTCC GGCAATGTCT CCGGTAGCAA ATGGACCGTC AACTAGTACA
961 CCAGGTTCTT CTTCACCTGT AGCAGGCGGA CCATCGTCAG GAAGCGGTAT TGCACCGGCG
1021 GCAAGCGGAC CATCTGGAAG CGCTATTGCA CCGGCGGCAG ACGGACCAAC
AGTAAGTATG
1081 TCACCGGGAC CATCGGCAGG AGGACCATTG GCAGGCGGAC CATCGGAGGG
AGCACCGGGA
1141 AGTAGTGCTT TAGGTCCCGG CGGCAGCAAT ACTCCGGCGG ACATCAATAC
GCCAGCTGGC
1201 GCACCAGAAA ACCCAAATTC ATTTGCAGTG AAGGCATTTA CTCCATCAGT
TGTACTTGTG
//
LOCUS gi|73808525|gb|DQ103386.1| Lycopersicon pimpinellifolium CT099
(CT099) gene, CT099-7272a allele, partial cds 1260 bp
DEFINITION gi|73808525|gb|DQ103386.1| Lycopersicon pimpinellifolium CT099
(CT099) gene, CT099-7272a allele, partial cds 1260 bp
TITLE gi|73808525|gb|DQ103386.1| Lycopersicon pimpinellifolium CT099
(CT099) gene, CT099-7272a allele, partial cds
ORIGIN
1 CTTAACCCTT CTGTTGATTA CAACACTTGG TCTCAGCATA TGAGATTTAT AATCAACGAC
61 TCTGTTCGTA AGTCTTAACA TGTCTCTCTT TATTTATTAT TGCTTGAAGA GTTTGAACAA
121 TATAATTGGT AAACGAGTTT TTTCTATAGT GTTGATACTT TTTTTTTTGT GTGTGTGCGC
181 TTTTTTTAGT GTTTAAGTAT AAGCAAGGAG CAGATTCTGT GTTGGAGGTG AGTAAGGATG
241 ATTATGATAA GTGCAATACT GGAAATCCAA TTAAGAAAAT GGAGGATGGA AACTCGATTT
301 TCACGTTGGA TCGGTCTGGT CCTTTTTACT TTATCAGTGG GAACAAAGAT AACTGTGATA
361 AAGGCCAAAA GTTGCAAATT GTTGTGATTT CTGCTAGGAA TCAAGGTAAA CCACCACAAA
421 CACCGGCACC GGCAGTTGCT CCACCTAGTA ATGGATCAAC TACACCATCT ACTCCTTCTG
481 GAGGTACGCG GAATCAGGAT TTTTATTTTA TTCCCGTT-C AATTTTACGT TGACATCTCC
541 TTATGTGGTT TGCGAGTTAT TGTATCTAAG GATAAAAATT ACATGATTCG ATTGAGAACT
601 TACGTGATTA ATGCAGGCTC AACTCCGGCA GCTGCTCCGT CTAAAGGATC GGGTACACCG
661 TCGGCACCTT CAGCTAACGC TCCGGCGGGA TCATCTAAAC CGGGTGCATC GTCACCTAAT
721 GGGGCACCGG TATCTACTCC TGCAGGTAAA TGTAGAATTT CGATTTGATG GATTCAACGT
781 TTAATTTCGT TCGATTTTTA ACGTATTGAA CGTAAAT-TG AATCATATAG TTTATCTGTT
841 TGTTCAGGTA AATCACCAAC CTCATCACCT ACACCTTCCG GTTCAACCGC ATCTCCACCA
901 TCTCCTGCGA CCGTAACTCC GGCAATGTCT CCGGTAGCAA ATGGACCGTC AACTAGTACA
961 CCAGGTTCTT CTTCACCTGT AGCAGGCGGA CCATCGTCAG GAAGCGGTAT TGCACCGTCG
1021 GCAAGCGGAC CATCTGGAAG CGCTATTGCA CCGGCGGCAG ACGGACCAAC AGTAAGTATG
1081 TCACCGGGAC CATCGGCAGG AGGACCATTG GCAGGCGGAC CATCGGAGGG AGCACCGGGA
PRACTICAS DE BIOINFORMATICA E.P. BIOLOGIA UNSA
1141 AGTAGTGCTT TAGGTCCCGG CGGCAGCAAT ACTCCGGCGG ACATCAATAC GCCAGCTGGC
1201 GCACCAGAAA ACCCAAATTC ATTTGCAGTG AAGGCATTTA CTCCATCAGT TGTACTTGTG
//
5. identificar los cambios presentes en las secuencias.(buscar mutaciones)
6. Aplicación del alineamiento multiple: con el archivo de alineamiento multiple,
construir un árbol filogenético usando MEGA.
6. Aplicación del alineamiento múltiple: con el archivo de alineamiento múltiple,
construir un árbol filogenético usando MEGA.
PRACTICAS DE BIOINFORMATICA E.P. BIOLOGIA UNSA
BIBLIGRAFIA:
[Link]
[Link]
Alineamiento de secuencias ([Link])
[Link] ›
