uereaeoaes, Merion ~ samp ‘CHILLED, FROZEN, PRECOOKED, ‘OR PREPARED FOODS, AND NUT MEATS. sr201 ‘AOAC Official Method 96.23 Microbiological Methods Firat Aoon 198 (Por the determizaon of arbi ite cout ot potable ‘umber of eoliform tacts, tad fscherichie oll tnd ‘Sapacoecusinpodscs has vo cooked ou, ad egotbe predess cooked andor bend Sao: bakery oft sls oe sumed; nd ingredients fd ota Eemplercllaced dng saab inspects of a pens ‘Sttblstent, uals specie decease ger for Sat rohet) ‘A. Mat and Reagents Ingres dreges sed pepe owing edie te prods of tay mace! i erp et sw at Sustiowry reused Usps crobatessubleor oop re, ACS reget pad eran chorea nd he ‘Sredy te Biologa! Sas Comms Toe mai Tor coats dchydned meta of ny band exalt forma may bused Tes echt fet or ey ad Bomib-romotng ales of matale opis (eg noel Pui cetanig tese with calor acer, Supiocces ‘Beda Sphyboces ‘Deteine pl tlre aocaving witht meter aed ssn mandir bof, 96424 (oe A100. Ada pl en econ y alg IM NWOH or [MHC 0 ste al pL Tenis ser caving ‘ses las rai 100 15mm, Pe is, (a) Plt cout par—Ser 936A) (e172, (8) Lawylnue pase rk Dasoie200 epee ype unceatiget of csi), $0 g NaCl 0 g nso, 2S gKJaP0, 275 2400, and 0 gstdun ay alae LHEO wi eae etfaersry, Dispense 10m ports 20» 150 a test tubes conning tered 10 7S ea ‘enon bs. aoc Saat 121°C: Fong 680.1 (© Bion gon lace (BCL bth Disole 100g peplne and 100 g cone ca S00 mi 0 Ad sole (70-73) of 2g deny egal or exile 20 0. Dintet97SLandasinpiio?¢ AMIS S mL Ola Sie gon anette Lib ibordough ets end ipene 0 porn 20» 150 men et ksi iaveed 10» 75cm emewtion bea Auocee 15 aot 12°C Fi pH. 72201 (@) Bosn mtn be agar (Levin) —So 940.3688) (ae ry (e) Bat Pater mab (tlie hiner sr ETGP4){i) Brame Spend 10D gtype 50g ek ext, 10 gyn etc 10 soon av, 10g ves, 0p LCI, 294200 pean 95 ml LO. Hea op {sunt agation to dish tdi comply. Dips 5 mi prio no sew-capped bots, Aosive 13 mn a 120°C Pin pl, 10202 2c. Stresses? = °C enn? AOAC CHPCALMETIORS OF AKANE 202) (2) rchnent—Baste EY wb etches BD Bh (clied Cat No 27910 or ql ac pepe fol Sak febegas ca in incu of need Hgch te(°100— ‘vi Atel nck ps aod wae yo or ies ln {rake pyclgea ne sie, 40.6890 17,39, ‘rs pepe nS To SD eo kere ad {Dat essa 1 psa class ston wy NEC sere 2 1. (3) Conlae main —Adl Sa warn she 8 sn al sal FC: Nw ng ai ‘Sapir 1-18 ta 100» Senn Pe des See a {et apes (O70) OF 39 cays ee we Ne ee dey pu doneeevemopeines Dry perk ‘tte buen mols (aceon con eee SOrC i ie rena an ap mrs Sor (2) ae a aha 4h 35° wi Basco ma tie er (2 ‘oer eth 1616 ba tenga Wi hd a fsoyaneal 50g Nac? $ KPO, ands p gcse ‘em ioesry Dense 1-20: damier (f5-8 cn, Autolv 5 nt 21°C. Pal Hi, 73.202 ig) EC brrh—Disave200 3 Type or typo pe doe fene) 13 ac ie alo er be ms ere 0 loam 40g KPO, 13 g KE\PO, nd 30 g NOC) Dips ra 1 10 sm nt coming eed me. 3 (G) Desiecated coagutare plarma (rabbit) ws EDL Recs ocedago nautica ects ‘rie ema elected congle plana (ai [NaIMEDTAU fin onceaton ferent sepia lol pans, (hy Bure pcs ot madtun)—Se 4 ue 100 ( (om) Burfi bufered phosphate dient) Se seionDiane 4 DAKH,FO,0.00mL Odi fren 175 IN Naan deen 1. Se (cn ie 135 net soe ww| AOC OFFIC METHCOS OF ANAL 201) earn Bln Wit i salto, desing eng 0 ‘ow felons dung aelving Autcae Ts int 2G. 8. Propwatin of Tet Setpensions ‘Prepare al inate wih 90 mist da pias 10 mt previ on es ober specie. Shae ll ant 25 tes i 30 cm ae. Pp tac clver ‘eoured vole Do et wet iver ot tl oa er empl deliver I dao se pipet 10 to ler | mt donate pet >t (0) Peemanorprepare foods —Uaebslncewithcpasity _ of 2 kp and renstiliy 0f 01 gv aepielly weigh 50 8 _eaved i bone) estporton tone igh spend lent _BrAa34S0mLluse A(m)) andolend2n (Pnecoary __ einer zen tet spe to remove 30 poten, old 18a 1I"°C) Nets la ston elapse om me et ap it edad un all taton ae in apropiate medi. Wet tet snpl com of weigh penn eee _ tetaanlendadh lune of umtuoqced race 10 = iin Tou lame Mederma opty aver ses ©) Tremaine aver an ger press Avge weigh 40 get poion into dee oA 30 aa, A, a Sine igen (Des twugh 30 oobi sn, _Letsand 35 nan ke ut before ming Sr tons | clone (0 nrmeal—Aspealy weigh 10 at penn nee, oO ew, (m2), eigen (Oe en “em ex) tin 10 son Let mnt 3-5 ln dae pt ee ling sel aoa an cone 6 Aerobie Pate Count Seep Pet die sione of 10, 108 1100, _ sig ple cut au, A) Only, 1100 acagh 1.1000 a cresctoaeat Mema08 ‘Chogerp sist: Pace 1 pope duo in ach pte and at ‘woten ap ole 42™4°0 wis Sin om ine eforga itn. este 48 = 2 35°C ead cous Geen ‘uitable ange 0-300 colonies) If pater do aot cota ‘St Sedan ro lon cman eer ofan Found. Avenge ots otabadand pertains 7202 ‘AOAC Official Method 966.24 Coliform Group and Escherichia cou "Merebseical MPH ethos "Prt ation 88 Firat acon 171 Seod.abemosybble number (MPN) sein me epee rth 9662840) Gee 17.20) wing esi 10, 70am 100 aoe wi tiple ete (Pt ut oat es an ls ie bp MPN deteaiaton wi Wf econ, bese wh 10 an ae 225 {35°C fr gs emo ar evidenced diplomat of bg itoertbeary ngs rescence whens hake oh nani ier forge fain 2 as ira Tee, si 3 mm op for sig tes fo BOLB, 9662340 ae 1720 omits ser re ma, mC wt IOC (Gee 1130} ast pe ein oe Tacabate BOLD froth 48 42 bat 35°C. Using MPN ‘Thle96624A,copese MPNontasxofmamrof tabs of BCE ‘rethpeceing psy codon ma Repent ‘lore ti Tact EC oth 5-278 455° 00°C covert Sobmeretrsern ba otto oatohightee Tole £00244. Moet probable numbers (IPN) pe get portion, wing’ tubes with ath of. 00, and 00% g partons