Cemal Gurkan

Eastern Mediterranean University, Medicine, Faculty Member

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Dr. Gurkan is a Berkeley (A.B.) and Cambridge (M.Phil. & Ph.D.) trained biochemist & molecular biologist. He is the director of the Turkish Cypriot DNA Laboratory in North Cyprus since 2009 and a scientific advisor to the Turkish Cypriot Member Office of the Committee on Missing Persons since 2006. His publication record largely comprises of proteomics, structural biology and genomics studies on human health and disease, such as on cystic fibrosis, breast cancer and forensic population genetics.

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Quantitative Proteomic Profiling of Matched Normal and Tumor Breast Tissues

by Nitin Rustogi, Andreas Hadjisavvas, and Cemal Gurkan

Journal of Proteome Research, 2010

Proteomic analysis of breast cancer tissue has proven difficult due to its inherent histological ... more Proteomic analysis of breast cancer tissue has proven difficult due to its inherent histological complexity. This pilot study presents preliminary evidence for the ability to differentiate adenoma and invasive carcinoma by measuring changes in proteomic profile of matched normal and disease tissues. A dual lysis buffer method was used to maximize protein extraction from each biopsy, proteins digested with trypsin, and the resulting peptides iTRAQ labeled. After combining, the peptide mixtures they were separated using preparative IEF followed by RP nanoHPLC. Following MALDI MS/MS and database searching, identified proteins were combined into a nonredundant list of 481 proteins with associated normal/tumor iTRAQ ratios for each patient. Proteins were categorized by location as blood, extracellular, and cellular, and the iTRAQ ratios were normalized to enable comparison between patients. Of those proteins significantly changed (upper or lower quartile) between matched normal and disease tissues, those from two invasive carcinoma patients had &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;gt;50% in common with each other but &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt;22% in common with an adenoma patient. In invasive carcinoma patients, several cellular and extracellular proteins that were significantly increased (Periostin, Small breast epithelial mucin) or decreased (Kinectin) have previously been associated with breast cancer, thereby supporting this approach for a larger disease-stage characterization effort.

Design and construction of membrane-acting immunotoxins for intracellular and secreted protein expression in Pichia pastoris

This work explored the design and construction of membrane-acting immunotoxins based on the activ... more This work explored the design and construction of membrane-acting immunotoxins based on the active toxin core of Cyt2Aa1 and single-chain antibody fragments C6.5 and C6ML3-9. Intracellular and secreted expression of these recombinant proteins were investigated using the methylotrophic yeast Pichia pastoris. Cyt2Aa1 is a cytolytic, haemolytic and diptericidal delta-endotoxin from Bacillus thuringiensis subspecies kyushuensis. C6.5 and C6ML3-9 are of human origin and the target is ErbB-2/HER-2, a surface glycoprotein which is overexpressed in approximately 30% of breast and ovarian cancers. C6ML3-9 is a mutant derivative of C6.5 with a six-fold increased affinity for the target antigen. An overlap extension PCR strategy was used successfully to create two immunolysin constructs based on the Cyt2Aa1 active toxin core and either C6.5 or C6ML3-9. The immunolysin design incorporated a flexible, soluble polypeptide linker between the toxin and the antibody domain, as well as a C-terminal h...

Kuzey Kıbrıs'ta ve Dünyada Adli Açıdan DNA Testleri

(6) Recombinant Production in Baculovirus-Infected Insect Cells and Purification of the Mammalian Sec13/Sec31 Complex

Meth Enzymology, 2005

Expression of the Bacillus thuringiensis Cyt2AaI toxin in Pichia pastoris using a synthetic gene construct

Biotechnology and Applied Biochemistry, 2003

Microsome-Based Assay for Analysis of ER to Golgi Transport in Mammalian Cells

Cemal Gurkan

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